Amino acid dipepetide frequency for Pseudonocardia sp. MH-G8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.113AlaAla: 24.113 ± 0.152	1.078AlaCys: 1.078 ± 0.021	8.634AlaAsp: 8.634 ± 0.059	8.606AlaGlu: 8.606 ± 0.067	3.761AlaPhe: 3.761 ± 0.042	14.673AlaGly: 14.673 ± 0.085	2.928AlaHis: 2.928 ± 0.036	4.113AlaIle: 4.113 ± 0.043	1.68AlaLys: 1.68 ± 0.033	15.541AlaLeu: 15.541 ± 0.075	2.681AlaMet: 2.681 ± 0.035	1.788AlaAsn: 1.788 ± 0.027	7.524AlaPro: 7.524 ± 0.063	3.955AlaGln: 3.955 ± 0.043	11.135AlaArg: 11.135 ± 0.08	5.819AlaSer: 5.819 ± 0.049	7.74AlaThr: 7.74 ± 0.052	14.078AlaVal: 14.078 ± 0.084	1.847AlaTrp: 1.847 ± 0.028	2.198AlaTyr: 2.198 ± 0.028	0.0AlaXaa: 0.0 ± 0.0
Cys	1.107CysAla: 1.107 ± 0.02	0.093CysCys: 0.093 ± 0.007	0.452CysAsp: 0.452 ± 0.013	0.375CysGlu: 0.375 ± 0.01	0.192CysPhe: 0.192 ± 0.008	0.927CysGly: 0.927 ± 0.019	0.187CysHis: 0.187 ± 0.009	0.181CysIle: 0.181 ± 0.007	0.077CysLys: 0.077 ± 0.005	0.64CysLeu: 0.64 ± 0.017	0.11CysMet: 0.11 ± 0.005	0.112CysAsn: 0.112 ± 0.006	0.422CysPro: 0.422 ± 0.012	0.142CysGln: 0.142 ± 0.008	0.627CysArg: 0.627 ± 0.015	0.459CysSer: 0.459 ± 0.015	0.489CysThr: 0.489 ± 0.015	0.618CysVal: 0.618 ± 0.014	0.118CysTrp: 0.118 ± 0.006	0.146CysTyr: 0.146 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	8.641AspAla: 8.641 ± 0.066	0.367AspCys: 0.367 ± 0.013	3.846AspAsp: 3.846 ± 0.044	4.027AspGlu: 4.027 ± 0.046	1.32AspPhe: 1.32 ± 0.028	6.398AspGly: 6.398 ± 0.061	1.373AspHis: 1.373 ± 0.023	1.596AspIle: 1.596 ± 0.025	0.608AspLys: 0.608 ± 0.022	6.475AspLeu: 6.475 ± 0.05	0.768AspMet: 0.768 ± 0.016	0.769AspAsn: 0.769 ± 0.021	4.821AspPro: 4.821 ± 0.048	1.395AspGln: 1.395 ± 0.026	5.302AspArg: 5.302 ± 0.051	2.095AspSer: 2.095 ± 0.038	2.856AspThr: 2.856 ± 0.036	5.804AspVal: 5.804 ± 0.054	0.809AspTrp: 0.809 ± 0.017	0.997AspTyr: 0.997 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	6.667GluAla: 6.667 ± 0.061	0.321GluCys: 0.321 ± 0.01	2.45GluAsp: 2.45 ± 0.035	2.782GluGlu: 2.782 ± 0.028	1.608GluPhe: 1.608 ± 0.022	3.699GluGly: 3.699 ± 0.037	1.714GluHis: 1.714 ± 0.025	2.21GluIle: 2.21 ± 0.031	0.88GluLys: 0.88 ± 0.021	7.038GluLeu: 7.038 ± 0.054	0.872GluMet: 0.872 ± 0.018	0.908GluAsn: 0.908 ± 0.02	3.569GluPro: 3.569 ± 0.041	2.377GluGln: 2.377 ± 0.032	5.701GluArg: 5.701 ± 0.052	2.382GluSer: 2.382 ± 0.037	2.426GluThr: 2.426 ± 0.031	4.884GluVal: 4.884 ± 0.047	0.818GluTrp: 0.818 ± 0.014	0.947GluTyr: 0.947 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	4.102PheAla: 4.102 ± 0.047	0.258PheCys: 0.258 ± 0.01	2.049PheAsp: 2.049 ± 0.03	1.281PheGlu: 1.281 ± 0.024	0.877PhePhe: 0.877 ± 0.021	3.196PheGly: 3.196 ± 0.039	0.576PheHis: 0.576 ± 0.016	0.662PheIle: 0.662 ± 0.018	0.252PheLys: 0.252 ± 0.009	2.745PheLeu: 2.745 ± 0.041	0.351PheMet: 0.351 ± 0.01	0.458PheAsn: 0.458 ± 0.013	1.456PhePro: 1.456 ± 0.025	0.568PheGln: 0.568 ± 0.015	1.878PheArg: 1.878 ± 0.028	1.385PheSer: 1.385 ± 0.023	1.861PheThr: 1.861 ± 0.026	2.594PheVal: 2.594 ± 0.03	0.452PheTrp: 0.452 ± 0.014	0.574PheTyr: 0.574 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	12.063GlyAla: 12.063 ± 0.078	0.897GlyCys: 0.897 ± 0.024	5.011GlyAsp: 5.011 ± 0.046	5.166GlyGlu: 5.166 ± 0.044	2.997GlyPhe: 2.997 ± 0.037	8.897GlyGly: 8.897 ± 0.077	2.197GlyHis: 2.197 ± 0.027	3.671GlyIle: 3.671 ± 0.044	1.546GlyLys: 1.546 ± 0.031	9.904GlyLeu: 9.904 ± 0.064	2.138GlyMet: 2.138 ± 0.029	1.501GlyAsn: 1.501 ± 0.028	5.517GlyPro: 5.517 ± 0.058	2.491GlyGln: 2.491 ± 0.036	8.183GlyArg: 8.183 ± 0.059	5.369GlySer: 5.369 ± 0.06	5.812GlyThr: 5.812 ± 0.047	8.603GlyVal: 8.603 ± 0.054	1.793GlyTrp: 1.793 ± 0.028	2.211GlyTyr: 2.211 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.836HisAla: 2.836 ± 0.031	0.189HisCys: 0.189 ± 0.008	1.466HisAsp: 1.466 ± 0.023	1.248HisGlu: 1.248 ± 0.018	0.546HisPhe: 0.546 ± 0.013	2.382HisGly: 2.382 ± 0.028	0.702HisHis: 0.702 ± 0.017	0.549HisIle: 0.549 ± 0.013	0.198HisLys: 0.198 ± 0.008	2.426HisLeu: 2.426 ± 0.031	0.301HisMet: 0.301 ± 0.011	0.325HisAsn: 0.325 ± 0.011	1.794HisPro: 1.794 ± 0.027	0.567HisGln: 0.567 ± 0.015	2.281HisArg: 2.281 ± 0.03	0.898HisSer: 0.898 ± 0.016	1.08HisThr: 1.08 ± 0.023	2.015HisVal: 2.015 ± 0.026	0.322HisTrp: 0.322 ± 0.011	0.432HisTyr: 0.432 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.251IleAla: 5.251 ± 0.046	0.264IleCys: 0.264 ± 0.009	2.344IleAsp: 2.344 ± 0.029	1.885IleGlu: 1.885 ± 0.027	0.731IlePhe: 0.731 ± 0.019	3.897IleGly: 3.897 ± 0.044	0.545IleHis: 0.545 ± 0.013	0.892IleIle: 0.892 ± 0.023	0.396IleLys: 0.396 ± 0.013	2.265IleLeu: 2.265 ± 0.033	0.444IleMet: 0.444 ± 0.012	0.636IleAsn: 0.636 ± 0.016	1.724IlePro: 1.724 ± 0.027	0.582IleGln: 0.582 ± 0.013	2.183IleArg: 2.183 ± 0.028	1.67IleSer: 1.67 ± 0.026	2.229IleThr: 2.229 ± 0.03	3.138IleVal: 3.138 ± 0.041	0.369IleTrp: 0.369 ± 0.013	0.548IleTyr: 0.548 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	1.697LysAla: 1.697 ± 0.036	0.058LysCys: 0.058 ± 0.004	0.656LysAsp: 0.656 ± 0.019	0.609LysGlu: 0.609 ± 0.018	0.288LysPhe: 0.288 ± 0.01	1.022LysGly: 1.022 ± 0.022	0.303LysHis: 0.303 ± 0.009	0.599LysIle: 0.599 ± 0.016	0.339LysLys: 0.339 ± 0.032	1.238LysLeu: 1.238 ± 0.021	0.215LysMet: 0.215 ± 0.009	0.24LysAsn: 0.24 ± 0.01	0.784LysPro: 0.784 ± 0.018	0.408LysGln: 0.408 ± 0.012	1.013LysArg: 1.013 ± 0.021	0.701LysSer: 0.701 ± 0.025	0.708LysThr: 0.708 ± 0.016	1.143LysVal: 1.143 ± 0.022	0.165LysTrp: 0.165 ± 0.008	0.239LysTyr: 0.239 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	16.795LeuAla: 16.795 ± 0.092	0.705LeuCys: 0.705 ± 0.016	7.124LeuAsp: 7.124 ± 0.062	4.729LeuGlu: 4.729 ± 0.047	2.709LeuPhe: 2.709 ± 0.04	10.013LeuGly: 10.013 ± 0.072	2.356LeuHis: 2.356 ± 0.03	3.021LeuIle: 3.021 ± 0.04	1.012LeuLys: 1.012 ± 0.022	11.894LeuLeu: 11.894 ± 0.095	1.477LeuMet: 1.477 ± 0.023	1.406LeuAsn: 1.406 ± 0.022	6.56LeuPro: 6.56 ± 0.056	2.434LeuGln: 2.434 ± 0.028	8.995LeuArg: 8.995 ± 0.062	5.0LeuSer: 5.0 ± 0.044	6.192LeuThr: 6.192 ± 0.049	10.796LeuVal: 10.796 ± 0.074	1.203LeuTrp: 1.203 ± 0.025	1.552LeuTyr: 1.552 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.263MetAla: 2.263 ± 0.028	0.114MetCys: 0.114 ± 0.006	0.76MetAsp: 0.76 ± 0.017	0.618MetGlu: 0.618 ± 0.014	0.474MetPhe: 0.474 ± 0.012	1.281MetGly: 1.281 ± 0.022	0.349MetHis: 0.349 ± 0.011	0.75MetIle: 0.75 ± 0.017	0.272MetLys: 0.272 ± 0.01	1.871MetLeu: 1.871 ± 0.033	0.275MetMet: 0.275 ± 0.01	0.384MetAsn: 0.384 ± 0.01	1.115MetPro: 1.115 ± 0.019	0.491MetGln: 0.491 ± 0.014	1.483MetArg: 1.483 ± 0.024	1.255MetSer: 1.255 ± 0.021	1.588MetThr: 1.588 ± 0.025	1.381MetVal: 1.381 ± 0.024	0.205MetTrp: 0.205 ± 0.008	0.262MetTyr: 0.262 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	1.982AsnAla: 1.982 ± 0.028	0.125AsnCys: 0.125 ± 0.007	0.782AsnAsp: 0.782 ± 0.02	0.67AsnGlu: 0.67 ± 0.016	0.447AsnPhe: 0.447 ± 0.013	1.543AsnGly: 1.543 ± 0.027	0.329AsnHis: 0.329 ± 0.011	0.546AsnIle: 0.546 ± 0.015	0.217AsnLys: 0.217 ± 0.009	1.571AsnLeu: 1.571 ± 0.022	0.232AsnMet: 0.232 ± 0.009	0.328AsnAsn: 0.328 ± 0.012	1.255AsnPro: 1.255 ± 0.021	0.416AsnGln: 0.416 ± 0.012	1.173AsnArg: 1.173 ± 0.025	0.736AsnSer: 0.736 ± 0.016	0.864AsnThr: 0.864 ± 0.017	1.343AsnVal: 1.343 ± 0.029	0.23AsnTrp: 0.23 ± 0.009	0.344AsnTyr: 0.344 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	9.174ProAla: 9.174 ± 0.071	0.316ProCys: 0.316 ± 0.011	4.902ProAsp: 4.902 ± 0.047	4.08ProGlu: 4.08 ± 0.045	1.591ProPhe: 1.591 ± 0.024	6.709ProGly: 6.709 ± 0.054	1.347ProHis: 1.347 ± 0.023	1.565ProIle: 1.565 ± 0.026	0.658ProLys: 0.658 ± 0.016	5.445ProLeu: 5.445 ± 0.057	1.068ProMet: 1.068 ± 0.018	0.803ProAsn: 0.803 ± 0.017	3.959ProPro: 3.959 ± 0.05	1.658ProGln: 1.658 ± 0.022	4.227ProArg: 4.227 ± 0.042	2.937ProSer: 2.937 ± 0.033	3.504ProThr: 3.504 ± 0.035	5.953ProVal: 5.953 ± 0.049	0.857ProTrp: 0.857 ± 0.019	1.058ProTyr: 1.058 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	3.524GlnAla: 3.524 ± 0.042	0.147GlnCys: 0.147 ± 0.008	1.275GlnAsp: 1.275 ± 0.024	1.401GlnGlu: 1.401 ± 0.022	0.733GlnPhe: 0.733 ± 0.017	1.936GlnGly: 1.936 ± 0.029	0.669GlnHis: 0.669 ± 0.015	1.052GlnIle: 1.052 ± 0.02	0.374GlnLys: 0.374 ± 0.013	3.201GlnLeu: 3.201 ± 0.033	0.46GlnMet: 0.46 ± 0.014	0.448GlnAsn: 0.448 ± 0.013	1.603GlnPro: 1.603 ± 0.028	1.153GlnGln: 1.153 ± 0.026	2.68GlnArg: 2.68 ± 0.03	1.067GlnSer: 1.067 ± 0.019	1.123GlnThr: 1.123 ± 0.021	2.665GlnVal: 2.665 ± 0.033	0.407GlnTrp: 0.407 ± 0.013	0.491GlnTyr: 0.491 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	10.986ArgAla: 10.986 ± 0.082	0.668ArgCys: 0.668 ± 0.015	4.6ArgAsp: 4.6 ± 0.054	4.749ArgGlu: 4.749 ± 0.042	2.555ArgPhe: 2.555 ± 0.034	6.443ArgGly: 6.443 ± 0.05	2.156ArgHis: 2.156 ± 0.028	3.23ArgIle: 3.23 ± 0.034	1.177ArgLys: 1.177 ± 0.025	8.649ArgLeu: 8.649 ± 0.062	1.869ArgMet: 1.869 ± 0.022	1.288ArgAsn: 1.288 ± 0.024	5.236ArgPro: 5.236 ± 0.054	2.1ArgGln: 2.1 ± 0.027	8.554ArgArg: 8.554 ± 0.071	4.526ArgSer: 4.526 ± 0.048	5.075ArgThr: 5.075 ± 0.041	6.605ArgVal: 6.605 ± 0.057	1.511ArgTrp: 1.511 ± 0.025	1.774ArgTyr: 1.774 ± 0.023	0.0ArgXaa: 0.0 ± 0.0
Ser	6.587SerAla: 6.587 ± 0.064	0.415SerCys: 0.415 ± 0.015	2.676SerAsp: 2.676 ± 0.044	2.239SerGlu: 2.239 ± 0.037	1.455SerPhe: 1.455 ± 0.022	5.71SerGly: 5.71 ± 0.064	0.892SerHis: 0.892 ± 0.016	1.6SerIle: 1.6 ± 0.024	0.623SerLys: 0.623 ± 0.019	4.352SerLeu: 4.352 ± 0.04	1.109SerMet: 1.109 ± 0.022	0.762SerAsn: 0.762 ± 0.015	2.979SerPro: 2.979 ± 0.034	1.088SerGln: 1.088 ± 0.02	3.67SerArg: 3.67 ± 0.047	2.68SerSer: 2.68 ± 0.049	3.093SerThr: 3.093 ± 0.034	4.263SerVal: 4.263 ± 0.041	0.891SerTrp: 0.891 ± 0.017	1.025SerTyr: 1.025 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	8.461ThrAla: 8.461 ± 0.065	0.414ThrCys: 0.414 ± 0.012	3.419ThrAsp: 3.419 ± 0.037	2.868ThrGlu: 2.868 ± 0.031	1.644ThrPhe: 1.644 ± 0.028	6.461ThrGly: 6.461 ± 0.052	1.097ThrHis: 1.097 ± 0.02	1.946ThrIle: 1.946 ± 0.024	0.666ThrLys: 0.666 ± 0.016	5.408ThrLeu: 5.408 ± 0.053	1.0ThrMet: 1.0 ± 0.019	0.856ThrAsn: 0.856 ± 0.017	3.828ThrPro: 3.828 ± 0.037	1.218ThrGln: 1.218 ± 0.023	4.011ThrArg: 4.011 ± 0.04	3.001ThrSer: 3.001 ± 0.034	3.811ThrThr: 3.811 ± 0.038	5.923ThrVal: 5.923 ± 0.043	0.862ThrTrp: 0.862 ± 0.018	1.06ThrTyr: 1.06 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	13.642ValAla: 13.642 ± 0.08	0.713ValCys: 0.713 ± 0.017	5.886ValAsp: 5.886 ± 0.047	5.292ValGlu: 5.292 ± 0.047	2.584ValPhe: 2.584 ± 0.035	7.899ValGly: 7.899 ± 0.053	2.128ValHis: 2.128 ± 0.033	2.868ValIle: 2.868 ± 0.044	1.008ValLys: 1.008 ± 0.019	11.545ValLeu: 11.545 ± 0.078	1.316ValMet: 1.316 ± 0.022	1.463ValAsn: 1.463 ± 0.027	5.852ValPro: 5.852 ± 0.051	2.341ValGln: 2.341 ± 0.033	7.569ValArg: 7.569 ± 0.059	4.326ValSer: 4.326 ± 0.045	5.529ValThr: 5.529 ± 0.043	10.177ValVal: 10.177 ± 0.077	1.118ValTrp: 1.118 ± 0.022	1.402ValTyr: 1.402 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	1.616TrpAla: 1.616 ± 0.026	0.156TrpCys: 0.156 ± 0.007	0.782TrpAsp: 0.782 ± 0.017	0.666TrpGlu: 0.666 ± 0.014	0.517TrpPhe: 0.517 ± 0.013	1.051TrpGly: 1.051 ± 0.021	0.365TrpHis: 0.365 ± 0.011	0.539TrpIle: 0.539 ± 0.013	0.235TrpLys: 0.235 ± 0.009	1.728TrpLeu: 1.728 ± 0.023	0.304TrpMet: 0.304 ± 0.011	0.339TrpAsn: 0.339 ± 0.012	0.804TrpPro: 0.804 ± 0.018	0.524TrpGln: 0.524 ± 0.013	1.345TrpArg: 1.345 ± 0.023	0.941TrpSer: 0.941 ± 0.02	0.962TrpThr: 0.962 ± 0.018	1.132TrpVal: 1.132 ± 0.022	0.371TrpTrp: 0.371 ± 0.011	0.278TrpTyr: 0.278 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.387TyrAla: 2.387 ± 0.029	0.176TyrCys: 0.176 ± 0.008	1.152TyrAsp: 1.152 ± 0.021	0.961TyrGlu: 0.961 ± 0.017	0.547TyrPhe: 0.547 ± 0.015	1.828TyrGly: 1.828 ± 0.029	0.342TyrHis: 0.342 ± 0.013	0.406TyrIle: 0.406 ± 0.013	0.206TyrLys: 0.206 ± 0.008	2.035TyrLeu: 2.035 ± 0.033	0.203TyrMet: 0.203 ± 0.009	0.323TyrAsn: 0.323 ± 0.009	1.01TyrPro: 1.01 ± 0.018	0.498TyrGln: 0.498 ± 0.014	1.739TyrArg: 1.739 ± 0.024	0.86TyrSer: 0.86 ± 0.016	1.014TyrThr: 1.014 ± 0.019	1.543TyrVal: 1.543 ± 0.028	0.308TyrTrp: 0.308 ± 0.01	0.414TyrTyr: 0.414 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 9083 proteins (2994516 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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