Escherichia phage haarsle

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Hanrivervirus; Escherichia virus haarsle

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9WV00|A0A6B9WV00_9CAUD Uncharacterized protein OS=Escherichia phage haarsle OX=2696402 GN=haarsle_1 PE=4 SV=1
MM1 pKa = 7.0SQDD4 pKa = 3.09GFFEE8 pKa = 4.19RR9 pKa = 11.84LQMAEE14 pKa = 3.8DD15 pKa = 3.52AGLNKK20 pKa = 9.59EE21 pKa = 4.11AALEE25 pKa = 3.94VAYY28 pKa = 9.67KK29 pKa = 10.29IRR31 pKa = 11.84TLDD34 pKa = 3.72DD35 pKa = 3.96ALGEE39 pKa = 3.83MDD41 pKa = 4.5MDD43 pKa = 4.56HH44 pKa = 6.84EE45 pKa = 5.04CGAAFADD52 pKa = 3.6PTMIVNDD59 pKa = 4.6CGCNFDD65 pKa = 4.9PNCPRR70 pKa = 11.84CFPFF74 pKa = 5.73

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9WY38|A0A6B9WY38_9CAUD Terminase small subunit OS=Escherichia phage haarsle OX=2696402 GN=haarsle_16 PE=4 SV=1
MM1 pKa = 7.72VEE3 pKa = 4.16LNDD6 pKa = 3.4IRR8 pKa = 11.84VGTKK12 pKa = 9.98FRR14 pKa = 11.84VTWADD19 pKa = 3.79EE20 pKa = 4.17YY21 pKa = 11.34CGVSKK26 pKa = 10.82GQVVTVDD33 pKa = 3.67SIYY36 pKa = 10.65RR37 pKa = 11.84GCSKK41 pKa = 10.62DD42 pKa = 3.29FRR44 pKa = 11.84RR45 pKa = 11.84PRR47 pKa = 11.84IKK49 pKa = 10.15NGYY52 pKa = 8.77IITRR56 pKa = 11.84RR57 pKa = 11.84FGFEE61 pKa = 3.49HH62 pKa = 6.91YY63 pKa = 10.56YY64 pKa = 10.73VVATQGVLIEE74 pKa = 4.1LQRR77 pKa = 11.84ISDD80 pKa = 3.56HH81 pKa = 6.57RR82 pKa = 11.84GCHH85 pKa = 4.96VKK87 pKa = 7.63TTKK90 pKa = 10.24IPFQRR95 pKa = 11.84ARR97 pKa = 11.84YY98 pKa = 7.96DD99 pKa = 3.15ARR101 pKa = 11.84RR102 pKa = 11.84MRR104 pKa = 11.84RR105 pKa = 11.84LARR108 pKa = 11.84NAIKK112 pKa = 10.27FKK114 pKa = 10.34KK115 pKa = 9.86HH116 pKa = 5.07GGNFYY121 pKa = 11.16RR122 pKa = 11.84MYY124 pKa = 10.86KK125 pKa = 10.38GIARR129 pKa = 11.84NAGKK133 pKa = 10.28

Molecular weight: 15.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14254

50

1141

192.6

21.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.18 ± 0.427

1.347 ± 0.172

6.132 ± 0.286

6.875 ± 0.248

3.887 ± 0.16

7.633 ± 0.307

1.782 ± 0.216

6.581 ± 0.199

7.415 ± 0.34

6.503 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.136 ± 0.219

4.771 ± 0.226

3.396 ± 0.198

3.957 ± 0.279

4.918 ± 0.238

6.111 ± 0.242

5.283 ± 0.243

6.875 ± 0.222

1.459 ± 0.118

3.76 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski