Amino acid dipepetide frequency for Escherichia phage haarsle

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.735AlaAla: 6.735 ± 0.983
1.052AlaCys: 1.052 ± 0.284
4.28AlaAsp: 4.28 ± 0.648
5.402AlaGlu: 5.402 ± 0.65
3.017AlaPhe: 3.017 ± 0.588
5.683AlaGly: 5.683 ± 0.691
1.052AlaHis: 1.052 ± 0.283
5.753AlaIle: 5.753 ± 0.648
7.297AlaLys: 7.297 ± 0.922
6.595AlaLeu: 6.595 ± 0.648
3.157AlaMet: 3.157 ± 0.419
3.227AlaAsn: 3.227 ± 0.538
1.964AlaPro: 1.964 ± 0.343
3.438AlaGln: 3.438 ± 0.501
3.929AlaArg: 3.929 ± 0.611
4.771AlaSer: 4.771 ± 0.517
4.49AlaThr: 4.49 ± 0.788
6.034AlaVal: 6.034 ± 0.682
1.403AlaTrp: 1.403 ± 0.264
2.526AlaTyr: 2.526 ± 0.435
0.0AlaXaa: 0.0 ± 0.0
Cys
0.982CysAla: 0.982 ± 0.269
0.281CysCys: 0.281 ± 0.222
1.684CysAsp: 1.684 ± 0.3
0.982CysGlu: 0.982 ± 0.274
0.351CysPhe: 0.351 ± 0.164
1.684CysGly: 1.684 ± 0.422
0.351CysHis: 0.351 ± 0.171
0.702CysIle: 0.702 ± 0.222
0.842CysLys: 0.842 ± 0.287
0.772CysLeu: 0.772 ± 0.244
0.491CysMet: 0.491 ± 0.178
0.561CysAsn: 0.561 ± 0.21
0.351CysPro: 0.351 ± 0.161
0.21CysGln: 0.21 ± 0.126
0.702CysArg: 0.702 ± 0.299
0.421CysSer: 0.421 ± 0.172
0.631CysThr: 0.631 ± 0.218
0.702CysVal: 0.702 ± 0.208
0.421CysTrp: 0.421 ± 0.162
0.351CysTyr: 0.351 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
5.192AspAla: 5.192 ± 0.609
0.561AspCys: 0.561 ± 0.228
4.069AspAsp: 4.069 ± 0.707
4.069AspGlu: 4.069 ± 0.541
2.806AspPhe: 2.806 ± 0.363
6.034AspGly: 6.034 ± 0.676
0.912AspHis: 0.912 ± 0.245
3.227AspIle: 3.227 ± 0.493
4.21AspLys: 4.21 ± 0.559
3.929AspLeu: 3.929 ± 0.435
1.894AspMet: 1.894 ± 0.357
2.596AspAsn: 2.596 ± 0.405
2.666AspPro: 2.666 ± 0.402
2.035AspGln: 2.035 ± 0.373
2.736AspArg: 2.736 ± 0.461
3.298AspSer: 3.298 ± 0.48
3.438AspThr: 3.438 ± 0.482
3.578AspVal: 3.578 ± 0.46
1.263AspTrp: 1.263 ± 0.35
2.806AspTyr: 2.806 ± 0.407
0.0AspXaa: 0.0 ± 0.0
Glu
6.104GluAla: 6.104 ± 0.792
0.772GluCys: 0.772 ± 0.253
3.298GluAsp: 3.298 ± 0.435
3.999GluGlu: 3.999 ± 0.542
3.508GluPhe: 3.508 ± 0.571
3.157GluGly: 3.157 ± 0.472
1.263GluHis: 1.263 ± 0.39
5.332GluIle: 5.332 ± 0.578
4.56GluLys: 4.56 ± 0.567
5.052GluLeu: 5.052 ± 0.683
2.806GluMet: 2.806 ± 0.437
3.719GluAsn: 3.719 ± 0.559
1.754GluPro: 1.754 ± 0.348
2.806GluGln: 2.806 ± 0.477
3.017GluArg: 3.017 ± 0.669
4.701GluSer: 4.701 ± 0.545
4.28GluThr: 4.28 ± 0.605
4.701GluVal: 4.701 ± 0.557
0.702GluTrp: 0.702 ± 0.222
3.227GluTyr: 3.227 ± 0.514
0.0GluXaa: 0.0 ± 0.0
Phe
2.596PheAla: 2.596 ± 0.406
0.21PheCys: 0.21 ± 0.138
3.017PheAsp: 3.017 ± 0.509
2.245PheGlu: 2.245 ± 0.374
1.403PhePhe: 1.403 ± 0.345
3.087PheGly: 3.087 ± 0.433
0.842PheHis: 0.842 ± 0.216
2.666PheIle: 2.666 ± 0.467
3.157PheLys: 3.157 ± 0.393
1.894PheLeu: 1.894 ± 0.395
1.333PheMet: 1.333 ± 0.347
2.596PheAsn: 2.596 ± 0.366
1.333PhePro: 1.333 ± 0.353
1.333PheGln: 1.333 ± 0.304
2.035PheArg: 2.035 ± 0.32
2.105PheSer: 2.105 ± 0.368
2.806PheThr: 2.806 ± 0.457
2.526PheVal: 2.526 ± 0.439
0.631PheTrp: 0.631 ± 0.191
1.052PheTyr: 1.052 ± 0.331
0.0PheXaa: 0.0 ± 0.0
Gly
4.841GlyAla: 4.841 ± 0.701
1.333GlyCys: 1.333 ± 0.291
5.192GlyAsp: 5.192 ± 0.869
5.332GlyGlu: 5.332 ± 0.635
3.368GlyPhe: 3.368 ± 0.513
7.297GlyGly: 7.297 ± 1.036
1.263GlyHis: 1.263 ± 0.315
4.28GlyIle: 4.28 ± 0.495
6.595GlyLys: 6.595 ± 0.604
5.543GlyLeu: 5.543 ± 0.507
2.736GlyMet: 2.736 ± 0.386
3.929GlyAsn: 3.929 ± 0.451
0.421GlyPro: 0.421 ± 0.171
2.105GlyGln: 2.105 ± 0.309
2.806GlyArg: 2.806 ± 0.365
5.473GlySer: 5.473 ± 0.485
2.806GlyThr: 2.806 ± 0.477
5.964GlyVal: 5.964 ± 0.647
1.403GlyTrp: 1.403 ± 0.279
3.648GlyTyr: 3.648 ± 0.525
0.0GlyXaa: 0.0 ± 0.0
His
0.702HisAla: 0.702 ± 0.258
0.351HisCys: 0.351 ± 0.17
1.263HisAsp: 1.263 ± 0.309
1.193HisGlu: 1.193 ± 0.324
0.561HisPhe: 0.561 ± 0.182
1.754HisGly: 1.754 ± 0.381
0.421HisHis: 0.421 ± 0.249
1.052HisIle: 1.052 ± 0.288
1.614HisLys: 1.614 ± 0.41
1.123HisLeu: 1.123 ± 0.279
0.281HisMet: 0.281 ± 0.189
0.772HisAsn: 0.772 ± 0.249
0.561HisPro: 0.561 ± 0.245
0.702HisGln: 0.702 ± 0.262
0.772HisArg: 0.772 ± 0.266
0.982HisSer: 0.982 ± 0.295
0.912HisThr: 0.912 ± 0.278
1.333HisVal: 1.333 ± 0.311
0.21HisTrp: 0.21 ± 0.118
1.263HisTyr: 1.263 ± 0.294
0.0HisXaa: 0.0 ± 0.0
Ile
6.385IleAla: 6.385 ± 0.646
1.193IleCys: 1.193 ± 0.307
4.841IleAsp: 4.841 ± 0.542
5.192IleGlu: 5.192 ± 0.584
2.035IlePhe: 2.035 ± 0.372
4.56IleGly: 4.56 ± 0.501
1.403IleHis: 1.403 ± 0.339
3.929IleIle: 3.929 ± 0.522
4.701IleLys: 4.701 ± 0.589
2.736IleLeu: 2.736 ± 0.351
1.964IleMet: 1.964 ± 0.379
3.648IleAsn: 3.648 ± 0.601
2.245IlePro: 2.245 ± 0.376
2.666IleGln: 2.666 ± 0.605
3.017IleArg: 3.017 ± 0.467
3.929IleSer: 3.929 ± 0.528
4.28IleThr: 4.28 ± 0.539
3.719IleVal: 3.719 ± 0.48
0.842IleTrp: 0.842 ± 0.319
2.526IleTyr: 2.526 ± 0.435
0.0IleXaa: 0.0 ± 0.0
Lys
6.595LysAla: 6.595 ± 1.074
0.842LysCys: 0.842 ± 0.285
4.49LysAsp: 4.49 ± 0.559
6.104LysGlu: 6.104 ± 0.666
2.947LysPhe: 2.947 ± 0.398
4.56LysGly: 4.56 ± 0.601
1.193LysHis: 1.193 ± 0.294
4.139LysIle: 4.139 ± 0.538
4.42LysLys: 4.42 ± 0.607
5.332LysLeu: 5.332 ± 0.618
3.368LysMet: 3.368 ± 0.53
3.298LysAsn: 3.298 ± 0.645
3.298LysPro: 3.298 ± 0.478
3.859LysGln: 3.859 ± 0.655
4.139LysArg: 4.139 ± 0.731
3.719LysSer: 3.719 ± 0.458
4.21LysThr: 4.21 ± 0.514
5.192LysVal: 5.192 ± 0.521
0.982LysTrp: 0.982 ± 0.309
1.473LysTyr: 1.473 ± 0.324
0.0LysXaa: 0.0 ± 0.0
Leu
5.613LeuAla: 5.613 ± 0.764
0.772LeuCys: 0.772 ± 0.246
3.227LeuAsp: 3.227 ± 0.474
3.929LeuGlu: 3.929 ± 0.528
1.544LeuPhe: 1.544 ± 0.34
3.859LeuGly: 3.859 ± 0.672
1.263LeuHis: 1.263 ± 0.323
5.122LeuIle: 5.122 ± 0.587
5.753LeuLys: 5.753 ± 0.592
3.929LeuLeu: 3.929 ± 0.543
2.245LeuMet: 2.245 ± 0.467
3.648LeuAsn: 3.648 ± 0.429
3.298LeuPro: 3.298 ± 0.547
2.035LeuGln: 2.035 ± 0.363
3.648LeuArg: 3.648 ± 0.428
4.28LeuSer: 4.28 ± 0.511
4.42LeuThr: 4.42 ± 0.514
3.227LeuVal: 3.227 ± 0.344
0.982LeuTrp: 0.982 ± 0.28
2.245LeuTyr: 2.245 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
3.227MetAla: 3.227 ± 0.411
0.351MetCys: 0.351 ± 0.143
1.544MetAsp: 1.544 ± 0.283
1.894MetGlu: 1.894 ± 0.451
0.631MetPhe: 0.631 ± 0.189
1.333MetGly: 1.333 ± 0.331
0.702MetHis: 0.702 ± 0.256
2.596MetIle: 2.596 ± 0.428
2.666MetLys: 2.666 ± 0.498
2.035MetLeu: 2.035 ± 0.43
1.052MetMet: 1.052 ± 0.271
1.263MetAsn: 1.263 ± 0.296
1.263MetPro: 1.263 ± 0.253
1.123MetGln: 1.123 ± 0.265
1.824MetArg: 1.824 ± 0.318
2.596MetSer: 2.596 ± 0.439
1.824MetThr: 1.824 ± 0.37
2.175MetVal: 2.175 ± 0.43
0.21MetTrp: 0.21 ± 0.121
1.052MetTyr: 1.052 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
4.35AsnAla: 4.35 ± 0.516
0.702AsnCys: 0.702 ± 0.274
3.298AsnAsp: 3.298 ± 0.505
3.578AsnGlu: 3.578 ± 0.425
1.894AsnPhe: 1.894 ± 0.246
5.613AsnGly: 5.613 ± 0.614
0.631AsnHis: 0.631 ± 0.207
2.806AsnIle: 2.806 ± 0.386
3.087AsnLys: 3.087 ± 0.527
3.017AsnLeu: 3.017 ± 0.442
0.912AsnMet: 0.912 ± 0.241
2.526AsnAsn: 2.526 ± 0.394
1.544AsnPro: 1.544 ± 0.291
2.035AsnGln: 2.035 ± 0.418
1.754AsnArg: 1.754 ± 0.358
2.596AsnSer: 2.596 ± 0.446
1.473AsnThr: 1.473 ± 0.204
3.438AsnVal: 3.438 ± 0.345
0.842AsnTrp: 0.842 ± 0.164
1.614AsnTyr: 1.614 ± 0.404
0.0AsnXaa: 0.0 ± 0.0
Pro
2.385ProAla: 2.385 ± 0.497
0.631ProCys: 0.631 ± 0.242
2.596ProAsp: 2.596 ± 0.571
3.648ProGlu: 3.648 ± 0.681
1.263ProPhe: 1.263 ± 0.25
2.806ProGly: 2.806 ± 0.453
0.561ProHis: 0.561 ± 0.183
1.894ProIle: 1.894 ± 0.339
1.333ProLys: 1.333 ± 0.224
1.754ProLeu: 1.754 ± 0.367
0.912ProMet: 0.912 ± 0.241
1.824ProAsn: 1.824 ± 0.382
1.403ProPro: 1.403 ± 0.345
0.982ProGln: 0.982 ± 0.237
1.614ProArg: 1.614 ± 0.389
1.473ProSer: 1.473 ± 0.335
1.754ProThr: 1.754 ± 0.337
3.017ProVal: 3.017 ± 0.48
0.561ProTrp: 0.561 ± 0.186
1.544ProTyr: 1.544 ± 0.344
0.0ProXaa: 0.0 ± 0.0
Gln
3.087GlnAla: 3.087 ± 0.487
0.561GlnCys: 0.561 ± 0.172
1.824GlnAsp: 1.824 ± 0.314
2.456GlnGlu: 2.456 ± 0.353
1.403GlnPhe: 1.403 ± 0.376
2.245GlnGly: 2.245 ± 0.37
0.631GlnHis: 0.631 ± 0.196
2.877GlnIle: 2.877 ± 0.47
2.385GlnLys: 2.385 ± 0.396
3.087GlnLeu: 3.087 ± 0.571
0.982GlnMet: 0.982 ± 0.34
1.684GlnAsn: 1.684 ± 0.379
1.263GlnPro: 1.263 ± 0.362
3.087GlnGln: 3.087 ± 0.772
2.315GlnArg: 2.315 ± 0.418
2.385GlnSer: 2.385 ± 0.404
1.754GlnThr: 1.754 ± 0.329
3.298GlnVal: 3.298 ± 0.402
0.421GlnTrp: 0.421 ± 0.194
1.754GlnTyr: 1.754 ± 0.356
0.0GlnXaa: 0.0 ± 0.0
Arg
3.578ArgAla: 3.578 ± 0.547
0.772ArgCys: 0.772 ± 0.273
2.245ArgAsp: 2.245 ± 0.413
3.438ArgGlu: 3.438 ± 0.418
1.964ArgPhe: 1.964 ± 0.339
3.157ArgGly: 3.157 ± 0.391
0.702ArgHis: 0.702 ± 0.22
3.929ArgIle: 3.929 ± 0.506
3.859ArgLys: 3.859 ± 0.554
2.596ArgLeu: 2.596 ± 0.432
1.614ArgMet: 1.614 ± 0.327
1.894ArgAsn: 1.894 ± 0.487
1.964ArgPro: 1.964 ± 0.413
2.035ArgGln: 2.035 ± 0.508
3.438ArgArg: 3.438 ± 0.527
2.736ArgSer: 2.736 ± 0.44
1.473ArgThr: 1.473 ± 0.296
4.841ArgVal: 4.841 ± 0.693
0.631ArgTrp: 0.631 ± 0.224
2.315ArgTyr: 2.315 ± 0.438
0.0ArgXaa: 0.0 ± 0.0
Ser
5.473SerAla: 5.473 ± 0.586
0.631SerCys: 0.631 ± 0.198
3.719SerAsp: 3.719 ± 0.506
4.771SerGlu: 4.771 ± 0.508
2.877SerPhe: 2.877 ± 0.39
5.332SerGly: 5.332 ± 0.44
1.193SerHis: 1.193 ± 0.293
4.701SerIle: 4.701 ± 0.658
4.28SerLys: 4.28 ± 0.489
4.139SerLeu: 4.139 ± 0.452
1.123SerMet: 1.123 ± 0.264
2.175SerAsn: 2.175 ± 0.511
2.105SerPro: 2.105 ± 0.289
2.456SerGln: 2.456 ± 0.397
2.666SerArg: 2.666 ± 0.419
3.298SerSer: 3.298 ± 0.472
2.315SerThr: 2.315 ± 0.383
4.49SerVal: 4.49 ± 0.471
0.912SerTrp: 0.912 ± 0.299
2.456SerTyr: 2.456 ± 0.432
0.0SerXaa: 0.0 ± 0.0
Thr
4.911ThrAla: 4.911 ± 0.851
0.561ThrCys: 0.561 ± 0.215
2.666ThrAsp: 2.666 ± 0.365
2.666ThrGlu: 2.666 ± 0.474
2.385ThrPhe: 2.385 ± 0.421
6.314ThrGly: 6.314 ± 0.723
0.351ThrHis: 0.351 ± 0.162
3.087ThrIle: 3.087 ± 0.508
3.508ThrLys: 3.508 ± 0.754
4.139ThrLeu: 4.139 ± 0.593
0.912ThrMet: 0.912 ± 0.259
2.947ThrAsn: 2.947 ± 0.422
2.315ThrPro: 2.315 ± 0.439
2.175ThrGln: 2.175 ± 0.284
1.684ThrArg: 1.684 ± 0.374
3.789ThrSer: 3.789 ± 0.596
2.035ThrThr: 2.035 ± 0.347
3.789ThrVal: 3.789 ± 0.452
0.772ThrTrp: 0.772 ± 0.221
1.824ThrTyr: 1.824 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
5.473ValAla: 5.473 ± 0.712
1.123ValCys: 1.123 ± 0.286
4.911ValAsp: 4.911 ± 0.425
4.49ValGlu: 4.49 ± 0.633
2.877ValPhe: 2.877 ± 0.508
3.999ValGly: 3.999 ± 0.49
1.544ValHis: 1.544 ± 0.432
4.56ValIle: 4.56 ± 0.493
5.893ValLys: 5.893 ± 0.625
4.21ValLeu: 4.21 ± 0.513
2.035ValMet: 2.035 ± 0.361
3.298ValAsn: 3.298 ± 0.491
1.964ValPro: 1.964 ± 0.533
2.035ValGln: 2.035 ± 0.36
3.508ValArg: 3.508 ± 0.512
4.981ValSer: 4.981 ± 0.695
4.841ValThr: 4.841 ± 0.615
4.139ValVal: 4.139 ± 0.451
0.842ValTrp: 0.842 ± 0.219
2.035ValTyr: 2.035 ± 0.342
0.0ValXaa: 0.0 ± 0.0
Trp
1.052TrpAla: 1.052 ± 0.34
0.14TrpCys: 0.14 ± 0.098
0.982TrpAsp: 0.982 ± 0.222
0.772TrpGlu: 0.772 ± 0.224
0.702TrpPhe: 0.702 ± 0.265
1.333TrpGly: 1.333 ± 0.28
0.772TrpHis: 0.772 ± 0.202
1.123TrpIle: 1.123 ± 0.261
0.912TrpLys: 0.912 ± 0.226
1.052TrpLeu: 1.052 ± 0.229
0.351TrpMet: 0.351 ± 0.127
0.491TrpAsn: 0.491 ± 0.206
0.351TrpPro: 0.351 ± 0.191
0.491TrpGln: 0.491 ± 0.161
1.403TrpArg: 1.403 ± 0.282
0.912TrpSer: 0.912 ± 0.263
0.631TrpThr: 0.631 ± 0.206
0.351TrpVal: 0.351 ± 0.129
0.21TrpTrp: 0.21 ± 0.115
0.561TrpTyr: 0.561 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.526TyrAla: 2.526 ± 0.533
0.631TyrCys: 0.631 ± 0.196
2.175TyrAsp: 2.175 ± 0.373
2.596TyrGlu: 2.596 ± 0.397
1.333TyrPhe: 1.333 ± 0.305
2.526TyrGly: 2.526 ± 0.403
0.772TyrHis: 0.772 ± 0.223
2.035TyrIle: 2.035 ± 0.338
3.087TyrLys: 3.087 ± 0.452
2.105TyrLeu: 2.105 ± 0.391
1.123TyrMet: 1.123 ± 0.258
1.614TyrAsn: 1.614 ± 0.33
1.894TyrPro: 1.894 ± 0.343
1.964TyrGln: 1.964 ± 0.302
2.175TyrArg: 2.175 ± 0.422
2.666TyrSer: 2.666 ± 0.53
2.456TyrThr: 2.456 ± 0.354
2.245TyrVal: 2.245 ± 0.301
0.351TyrTrp: 0.351 ± 0.17
1.333TyrTyr: 1.333 ± 0.345
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14254 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski