Bosea sp. Root381

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5096 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q9IME3|A0A0Q9IME3_9BRAD Peptidase M16 OS=Bosea sp. Root381 OX=1736524 GN=ASE63_02205 PE=3 SV=1
MM1 pKa = 7.47TNLTIYY7 pKa = 10.23AHH9 pKa = 7.33RR10 pKa = 11.84GTNPYY15 pKa = 9.12PDD17 pKa = 4.25HH18 pKa = 7.4SRR20 pKa = 11.84DD21 pKa = 3.54AYY23 pKa = 10.05VWAVNYY29 pKa = 10.01GADD32 pKa = 4.56FIEE35 pKa = 5.21PDD37 pKa = 4.04LFLTKK42 pKa = 10.54DD43 pKa = 3.49GVLVASHH50 pKa = 7.49DD51 pKa = 3.64NHH53 pKa = 6.56NYY55 pKa = 10.82ANLTYY60 pKa = 10.62AEE62 pKa = 4.38AKK64 pKa = 10.32AIEE67 pKa = 4.33PALMTFGEE75 pKa = 4.56VIEE78 pKa = 4.7IAKK81 pKa = 10.0QMSIEE86 pKa = 4.14TGRR89 pKa = 11.84QIGVIPEE96 pKa = 4.24TKK98 pKa = 9.4SANYY102 pKa = 7.54ATSEE106 pKa = 3.93AVIRR110 pKa = 11.84DD111 pKa = 4.54LIAHH115 pKa = 7.39DD116 pKa = 3.95FTDD119 pKa = 4.04PNLVVIQSFQSSNLKK134 pKa = 8.86MLHH137 pKa = 5.04EE138 pKa = 5.04TIMPQYY144 pKa = 10.95GVDD147 pKa = 3.83LPLAFLGYY155 pKa = 10.6NMSAATIADD164 pKa = 3.73TATYY168 pKa = 11.04ADD170 pKa = 4.57IIAPNQAALTAAGIEE185 pKa = 4.1AAHH188 pKa = 6.27AAGLKK193 pKa = 9.28VVTWTVLGTEE203 pKa = 4.12AQIQRR208 pKa = 11.84LVDD211 pKa = 4.08LGVDD215 pKa = 3.85GVFVDD220 pKa = 4.02ATNTARR226 pKa = 11.84EE227 pKa = 4.29ALSKK231 pKa = 10.88INGVTVGYY239 pKa = 8.46GTEE242 pKa = 4.34GDD244 pKa = 4.28DD245 pKa = 5.06EE246 pKa = 4.46IAGTDD251 pKa = 3.96GDD253 pKa = 4.09DD254 pKa = 5.34LIYY257 pKa = 11.13GMAGDD262 pKa = 5.05DD263 pKa = 4.49EE264 pKa = 5.21ISAGDD269 pKa = 3.91GNDD272 pKa = 3.2VVYY275 pKa = 11.03GDD277 pKa = 4.76AGDD280 pKa = 5.1DD281 pKa = 3.86IIEE284 pKa = 4.54GGAGNDD290 pKa = 3.55VLVGGAGDD298 pKa = 4.29DD299 pKa = 3.88EE300 pKa = 5.22LFGGAGDD307 pKa = 4.45DD308 pKa = 3.75VLKK311 pKa = 11.2GGVGDD316 pKa = 4.78DD317 pKa = 4.53LLDD320 pKa = 4.34GGDD323 pKa = 4.15GVDD326 pKa = 3.22TADD329 pKa = 4.67YY330 pKa = 11.13SDD332 pKa = 4.09DD333 pKa = 3.88TAGVTVDD340 pKa = 4.58LSAGTASGDD349 pKa = 3.53EE350 pKa = 4.41AGDD353 pKa = 4.2DD354 pKa = 3.87EE355 pKa = 6.22LISS358 pKa = 3.69

Molecular weight: 37.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q9I4U9|A0A0Q9I4U9_9BRAD Chaperone protein DnaK OS=Bosea sp. Root381 OX=1736524 GN=dnaK PE=2 SV=1
MM1 pKa = 7.69LSPIRR6 pKa = 11.84AAGCKK11 pKa = 9.13RR12 pKa = 11.84WPCGAEE18 pKa = 3.32PAARR22 pKa = 11.84HH23 pKa = 4.95TKK25 pKa = 9.1RR26 pKa = 11.84TFSDD30 pKa = 2.96NAADD34 pKa = 3.5KK35 pKa = 10.92RR36 pKa = 11.84VGQEE40 pKa = 3.57ALRR43 pKa = 11.84AMIQIKK49 pKa = 10.04GPRR52 pKa = 11.84DD53 pKa = 3.32FAAGCTFILIALFYY67 pKa = 10.67LWFGRR72 pKa = 11.84NLSIGSSSFMEE83 pKa = 4.14AGYY86 pKa = 9.8FPRR89 pKa = 11.84LAAVALALFGAIIAVRR105 pKa = 11.84AVAIDD110 pKa = 4.07GPGLEE115 pKa = 4.12PWAWRR120 pKa = 11.84PLLILTGCVVLFGLIITRR138 pKa = 11.84AGLVLTTVLVVVAMSFAGGRR158 pKa = 11.84LSWTRR163 pKa = 11.84LAGFVAALVVFMVGLFHH180 pKa = 7.51FGLGLAIPVWPQQ192 pKa = 2.47

Molecular weight: 20.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5096

0

5096

1611244

41

5166

316.2

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.498 ± 0.045

0.781 ± 0.011

5.361 ± 0.03

5.553 ± 0.033

3.672 ± 0.023

9.064 ± 0.06

1.896 ± 0.016

5.096 ± 0.024

3.069 ± 0.03

10.431 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.02

2.354 ± 0.028

5.352 ± 0.034

3.025 ± 0.016

7.232 ± 0.04

5.294 ± 0.033

5.193 ± 0.037

7.448 ± 0.031

1.295 ± 0.017

2.041 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski