Amino acid dipepetide frequency for Bosea sp. Root381

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.749AlaAla: 19.749 ± 0.162	1.106AlaCys: 1.106 ± 0.03	6.628AlaAsp: 6.628 ± 0.075	8.099AlaGlu: 8.099 ± 0.087	4.836AlaPhe: 4.836 ± 0.061	12.479AlaGly: 12.479 ± 0.145	2.182AlaHis: 2.182 ± 0.041	7.012AlaIle: 7.012 ± 0.082	4.232AlaLys: 4.232 ± 0.064	15.172AlaLeu: 15.172 ± 0.136	3.698AlaMet: 3.698 ± 0.052	2.828AlaAsn: 2.828 ± 0.047	6.204AlaPro: 6.204 ± 0.079	4.38AlaGln: 4.38 ± 0.058	9.588AlaArg: 9.588 ± 0.099	6.851AlaSer: 6.851 ± 0.09	6.563AlaThr: 6.563 ± 0.089	9.235AlaVal: 9.235 ± 0.078	1.499AlaTrp: 1.499 ± 0.03	2.638AlaTyr: 2.638 ± 0.043	0.001AlaXaa: 0.001 ± 0.001
Cys	0.956CysAla: 0.956 ± 0.026	0.102CysCys: 0.102 ± 0.009	0.468CysAsp: 0.468 ± 0.019	0.43CysGlu: 0.43 ± 0.015	0.294CysPhe: 0.294 ± 0.013	0.923CysGly: 0.923 ± 0.028	0.199CysHis: 0.199 ± 0.012	0.412CysIle: 0.412 ± 0.017	0.159CysLys: 0.159 ± 0.01	0.789CysLeu: 0.789 ± 0.025	0.133CysMet: 0.133 ± 0.01	0.156CysAsn: 0.156 ± 0.01	0.369CysPro: 0.369 ± 0.015	0.19CysGln: 0.19 ± 0.009	0.609CysArg: 0.609 ± 0.022	0.404CysSer: 0.404 ± 0.017	0.358CysThr: 0.358 ± 0.018	0.586CysVal: 0.586 ± 0.022	0.115CysTrp: 0.115 ± 0.008	0.161CysTyr: 0.161 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.931AspAla: 6.931 ± 0.075	0.431AspCys: 0.431 ± 0.017	2.846AspAsp: 2.846 ± 0.073	3.386AspGlu: 3.386 ± 0.05	2.022AspPhe: 2.022 ± 0.039	5.292AspGly: 5.292 ± 0.086	1.087AspHis: 1.087 ± 0.027	2.811AspIle: 2.811 ± 0.039	1.607AspLys: 1.607 ± 0.04	5.675AspLeu: 5.675 ± 0.058	1.121AspMet: 1.121 ± 0.027	1.085AspAsn: 1.085 ± 0.031	3.357AspPro: 3.357 ± 0.051	1.465AspGln: 1.465 ± 0.035	3.971AspArg: 3.971 ± 0.065	2.026AspSer: 2.026 ± 0.036	2.343AspThr: 2.343 ± 0.045	3.952AspVal: 3.952 ± 0.049	0.896AspTrp: 0.896 ± 0.022	1.306AspTyr: 1.306 ± 0.03	0.002AspXaa: 0.002 ± 0.002
Glu	8.403GluAla: 8.403 ± 0.094	0.32GluCys: 0.32 ± 0.014	2.372GluAsp: 2.372 ± 0.042	2.85GluGlu: 2.85 ± 0.047	1.643GluPhe: 1.643 ± 0.033	4.377GluGly: 4.377 ± 0.054	1.112GluHis: 1.112 ± 0.03	3.548GluIle: 3.548 ± 0.049	1.979GluLys: 1.979 ± 0.04	5.295GluLeu: 5.295 ± 0.064	1.409GluMet: 1.409 ± 0.032	1.32GluAsn: 1.32 ± 0.031	3.081GluPro: 3.081 ± 0.055	1.921GluGln: 1.921 ± 0.039	5.225GluArg: 5.225 ± 0.068	2.29GluSer: 2.29 ± 0.04	3.339GluThr: 3.339 ± 0.046	3.643GluVal: 3.643 ± 0.048	0.633GluTrp: 0.633 ± 0.019	0.772GluTyr: 0.772 ± 0.023	0.001GluXaa: 0.001 ± 0.001
Phe	4.983PheAla: 4.983 ± 0.059	0.37PheCys: 0.37 ± 0.016	2.51PheAsp: 2.51 ± 0.039	2.034PheGlu: 2.034 ± 0.035	1.344PhePhe: 1.344 ± 0.032	3.857PheGly: 3.857 ± 0.054	0.733PheHis: 0.733 ± 0.021	1.635PheIle: 1.635 ± 0.033	1.011PheLys: 1.011 ± 0.025	3.281PheLeu: 3.281 ± 0.049	0.825PheMet: 0.825 ± 0.028	0.947PheAsn: 0.947 ± 0.028	1.632PhePro: 1.632 ± 0.037	0.973PheGln: 0.973 ± 0.025	2.257PheArg: 2.257 ± 0.038	2.115PheSer: 2.115 ± 0.04	1.882PheThr: 1.882 ± 0.037	2.962PheVal: 2.962 ± 0.047	0.543PheTrp: 0.543 ± 0.021	0.83PheTyr: 0.83 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	10.457GlyAla: 10.457 ± 0.138	0.825GlyCys: 0.825 ± 0.026	4.398GlyAsp: 4.398 ± 0.082	5.145GlyGlu: 5.145 ± 0.065	3.849GlyPhe: 3.849 ± 0.056	8.384GlyGly: 8.384 ± 0.223	1.897GlyHis: 1.897 ± 0.04	4.67GlyIle: 4.67 ± 0.059	3.143GlyLys: 3.143 ± 0.056	9.839GlyLeu: 9.839 ± 0.09	2.223GlyMet: 2.223 ± 0.04	2.219GlyAsn: 2.219 ± 0.074	3.81GlyPro: 3.81 ± 0.052	2.978GlyGln: 2.978 ± 0.042	6.813GlyArg: 6.813 ± 0.081	5.096GlySer: 5.096 ± 0.095	4.906GlyThr: 4.906 ± 0.107	6.319GlyVal: 6.319 ± 0.068	1.414GlyTrp: 1.414 ± 0.03	2.252GlyTyr: 2.252 ± 0.041	0.001GlyXaa: 0.001 ± 0.001
His	2.356HisAla: 2.356 ± 0.039	0.203HisCys: 0.203 ± 0.012	1.178HisAsp: 1.178 ± 0.027	0.996HisGlu: 0.996 ± 0.027	0.773HisPhe: 0.773 ± 0.021	1.953HisGly: 1.953 ± 0.04	0.517HisHis: 0.517 ± 0.024	0.84HisIle: 0.84 ± 0.028	0.447HisLys: 0.447 ± 0.016	1.964HisLeu: 1.964 ± 0.044	0.403HisMet: 0.403 ± 0.015	0.412HisAsn: 0.412 ± 0.016	1.271HisPro: 1.271 ± 0.033	0.497HisGln: 0.497 ± 0.018	1.366HisArg: 1.366 ± 0.032	0.856HisSer: 0.856 ± 0.024	0.659HisThr: 0.659 ± 0.021	1.505HisVal: 1.505 ± 0.028	0.302HisTrp: 0.302 ± 0.013	0.465HisTyr: 0.465 ± 0.019	0.001HisXaa: 0.001 ± 0.001
Ile	7.877IleAla: 7.877 ± 0.077	0.495IleCys: 0.495 ± 0.019	3.451IleAsp: 3.451 ± 0.044	3.475IleGlu: 3.475 ± 0.051	1.629IlePhe: 1.629 ± 0.033	5.334IleGly: 5.334 ± 0.071	0.838IleHis: 0.838 ± 0.025	2.033IleIle: 2.033 ± 0.041	1.378IleLys: 1.378 ± 0.033	4.427IleLeu: 4.427 ± 0.056	0.908IleMet: 0.908 ± 0.023	1.199IleAsn: 1.199 ± 0.031	2.318IlePro: 2.318 ± 0.043	1.136IleGln: 1.136 ± 0.028	3.183IleArg: 3.183 ± 0.048	2.588IleSer: 2.588 ± 0.043	2.535IleThr: 2.535 ± 0.04	4.547IleVal: 4.547 ± 0.057	0.605IleTrp: 0.605 ± 0.022	1.005IleTyr: 1.005 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.536LysAla: 4.536 ± 0.063	0.119LysCys: 0.119 ± 0.009	1.572LysAsp: 1.572 ± 0.04	1.408LysGlu: 1.408 ± 0.032	0.794LysPhe: 0.794 ± 0.023	2.652LysGly: 2.652 ± 0.049	0.54LysHis: 0.54 ± 0.02	1.497LysIle: 1.497 ± 0.034	1.004LysLys: 1.004 ± 0.031	3.405LysLeu: 3.405 ± 0.047	0.608LysMet: 0.608 ± 0.021	0.715LysAsn: 0.715 ± 0.025	2.162LysPro: 2.162 ± 0.042	0.937LysGln: 0.937 ± 0.025	2.301LysArg: 2.301 ± 0.044	1.597LysSer: 1.597 ± 0.034	1.732LysThr: 1.732 ± 0.039	2.33LysVal: 2.33 ± 0.05	0.299LysTrp: 0.299 ± 0.015	0.482LysTyr: 0.482 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	15.607LeuAla: 15.607 ± 0.142	0.84LeuCys: 0.84 ± 0.022	6.167LeuAsp: 6.167 ± 0.073	4.866LeuGlu: 4.866 ± 0.059	3.651LeuPhe: 3.651 ± 0.058	9.157LeuGly: 9.157 ± 0.094	1.757LeuHis: 1.757 ± 0.033	5.046LeuIle: 5.046 ± 0.062	3.528LeuLys: 3.528 ± 0.053	10.053LeuLeu: 10.053 ± 0.103	2.283LeuMet: 2.283 ± 0.044	2.355LeuAsn: 2.355 ± 0.042	5.953LeuPro: 5.953 ± 0.063	2.654LeuGln: 2.654 ± 0.045	7.225LeuArg: 7.225 ± 0.077	6.447LeuSer: 6.447 ± 0.066	5.632LeuThr: 5.632 ± 0.084	8.006LeuVal: 8.006 ± 0.077	1.203LeuTrp: 1.203 ± 0.029	1.877LeuTyr: 1.877 ± 0.037	0.001LeuXaa: 0.001 ± 0.001
Met	3.114MetAla: 3.114 ± 0.048	0.139MetCys: 0.139 ± 0.009	0.954MetAsp: 0.954 ± 0.027	0.922MetGlu: 0.922 ± 0.027	0.652MetPhe: 0.652 ± 0.024	1.663MetGly: 1.663 ± 0.035	0.38MetHis: 0.38 ± 0.015	1.232MetIle: 1.232 ± 0.034	0.894MetLys: 0.894 ± 0.023	2.64MetLeu: 2.64 ± 0.045	0.57MetMet: 0.57 ± 0.023	0.686MetAsn: 0.686 ± 0.02	1.541MetPro: 1.541 ± 0.033	0.764MetGln: 0.764 ± 0.021	1.793MetArg: 1.793 ± 0.036	1.573MetSer: 1.573 ± 0.029	1.815MetThr: 1.815 ± 0.037	1.645MetVal: 1.645 ± 0.033	0.174MetTrp: 0.174 ± 0.011	0.251MetTyr: 0.251 ± 0.014	0.001MetXaa: 0.001 ± 0.001
Asn	3.09AsnAla: 3.09 ± 0.058	0.205AsnCys: 0.205 ± 0.012	1.234AsnAsp: 1.234 ± 0.045	1.079AsnGlu: 1.079 ± 0.029	0.837AsnPhe: 0.837 ± 0.022	2.294AsnGly: 2.294 ± 0.056	0.439AsnHis: 0.439 ± 0.017	1.185AsnIle: 1.185 ± 0.03	0.589AsnLys: 0.589 ± 0.021	2.471AsnLeu: 2.471 ± 0.041	0.518AsnMet: 0.518 ± 0.017	0.656AsnAsn: 0.656 ± 0.034	1.683AsnPro: 1.683 ± 0.034	0.659AsnGln: 0.659 ± 0.021	1.608AsnArg: 1.608 ± 0.031	1.087AsnSer: 1.087 ± 0.035	1.117AsnThr: 1.117 ± 0.031	1.788AsnVal: 1.788 ± 0.041	0.407AsnTrp: 0.407 ± 0.016	0.591AsnTyr: 0.591 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.356ProAla: 7.356 ± 0.087	0.285ProCys: 0.285 ± 0.014	3.36ProAsp: 3.36 ± 0.052	3.721ProGlu: 3.721 ± 0.058	2.045ProPhe: 2.045 ± 0.038	5.073ProGly: 5.073 ± 0.053	1.068ProHis: 1.068 ± 0.025	2.293ProIle: 2.293 ± 0.041	1.614ProLys: 1.614 ± 0.039	5.026ProLeu: 5.026 ± 0.064	1.182ProMet: 1.182 ± 0.025	1.273ProAsn: 1.273 ± 0.026	2.78ProPro: 2.78 ± 0.059	1.75ProGln: 1.75 ± 0.036	3.297ProArg: 3.297 ± 0.054	2.798ProSer: 2.798 ± 0.04	2.495ProThr: 2.495 ± 0.039	4.281ProVal: 4.281 ± 0.055	0.685ProTrp: 0.685 ± 0.021	1.136ProTyr: 1.136 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.561GlnAla: 4.561 ± 0.058	0.165GlnCys: 0.165 ± 0.009	1.419GlnAsp: 1.419 ± 0.031	1.376GlnGlu: 1.376 ± 0.032	0.952GlnPhe: 0.952 ± 0.028	2.474GlnGly: 2.474 ± 0.04	0.582GlnHis: 0.582 ± 0.021	1.763GlnIle: 1.763 ± 0.038	0.913GlnLys: 0.913 ± 0.025	2.79GlnLeu: 2.79 ± 0.047	0.753GlnMet: 0.753 ± 0.024	0.794GlnAsn: 0.794 ± 0.024	1.848GlnPro: 1.848 ± 0.037	1.159GlnGln: 1.159 ± 0.035	2.527GlnArg: 2.527 ± 0.047	1.616GlnSer: 1.616 ± 0.04	1.526GlnThr: 1.526 ± 0.035	2.183GlnVal: 2.183 ± 0.035	0.333GlnTrp: 0.333 ± 0.014	0.52GlnTyr: 0.52 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	8.235ArgAla: 8.235 ± 0.102	0.502ArgCys: 0.502 ± 0.018	4.094ArgAsp: 4.094 ± 0.051	4.323ArgGlu: 4.323 ± 0.068	3.055ArgPhe: 3.055 ± 0.04	5.056ArgGly: 5.056 ± 0.061	1.704ArgHis: 1.704 ± 0.035	4.199ArgIle: 4.199 ± 0.054	2.184ArgLys: 2.184 ± 0.045	8.359ArgLeu: 8.359 ± 0.093	1.879ArgMet: 1.879 ± 0.036	1.755ArgAsn: 1.755 ± 0.034	3.852ArgPro: 3.852 ± 0.059	2.597ArgGln: 2.597 ± 0.045	5.952ArgArg: 5.952 ± 0.08	3.961ArgSer: 3.961 ± 0.058	3.224ArgThr: 3.224 ± 0.047	4.763ArgVal: 4.763 ± 0.062	0.974ArgTrp: 0.974 ± 0.026	1.654ArgTyr: 1.654 ± 0.035	0.002ArgXaa: 0.002 ± 0.001
Ser	6.35SerAla: 6.35 ± 0.081	0.432SerCys: 0.432 ± 0.018	2.672SerAsp: 2.672 ± 0.036	2.705SerGlu: 2.705 ± 0.048	2.422SerPhe: 2.422 ± 0.039	5.718SerGly: 5.718 ± 0.106	1.035SerHis: 1.035 ± 0.025	2.594SerIle: 2.594 ± 0.048	1.36SerLys: 1.36 ± 0.036	5.647SerLeu: 5.647 ± 0.069	1.197SerMet: 1.197 ± 0.026	1.207SerAsn: 1.207 ± 0.036	2.795SerPro: 2.795 ± 0.042	1.607SerGln: 1.607 ± 0.032	3.71SerArg: 3.71 ± 0.048	2.828SerSer: 2.828 ± 0.046	2.614SerThr: 2.614 ± 0.051	3.953SerVal: 3.953 ± 0.05	0.843SerTrp: 0.843 ± 0.022	1.254SerTyr: 1.254 ± 0.033	0.001SerXaa: 0.001 ± 0.001
Thr	6.515ThrAla: 6.515 ± 0.086	0.377ThrCys: 0.377 ± 0.016	2.496ThrAsp: 2.496 ± 0.045	2.501ThrGlu: 2.501 ± 0.046	1.881ThrPhe: 1.881 ± 0.036	5.199ThrGly: 5.199 ± 0.09	0.909ThrHis: 0.909 ± 0.025	2.985ThrIle: 2.985 ± 0.044	1.381ThrLys: 1.381 ± 0.033	6.048ThrLeu: 6.048 ± 0.084	1.165ThrMet: 1.165 ± 0.028	1.189ThrAsn: 1.189 ± 0.039	3.16ThrPro: 3.16 ± 0.047	1.412ThrGln: 1.412 ± 0.029	3.301ThrArg: 3.301 ± 0.052	2.636ThrSer: 2.636 ± 0.056	2.829ThrThr: 2.829 ± 0.056	4.286ThrVal: 4.286 ± 0.064	0.593ThrTrp: 0.593 ± 0.018	1.069ThrTyr: 1.069 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	10.159ValAla: 10.159 ± 0.099	0.571ValCys: 0.571 ± 0.019	3.886ValAsp: 3.886 ± 0.058	4.58ValGlu: 4.58 ± 0.055	2.764ValPhe: 2.764 ± 0.043	5.725ValGly: 5.725 ± 0.061	1.282ValHis: 1.282 ± 0.033	3.869ValIle: 3.869 ± 0.051	2.318ValLys: 2.318 ± 0.041	7.638ValLeu: 7.638 ± 0.078	1.864ValMet: 1.864 ± 0.037	1.807ValAsn: 1.807 ± 0.039	3.903ValPro: 3.903 ± 0.047	1.975ValGln: 1.975 ± 0.036	4.667ValArg: 4.667 ± 0.054	4.332ValSer: 4.332 ± 0.05	4.629ValThr: 4.629 ± 0.06	6.279ValVal: 6.279 ± 0.071	0.851ValTrp: 0.851 ± 0.024	1.384ValTyr: 1.384 ± 0.033	0.001ValXaa: 0.001 ± 0.001
Trp	1.242TrpAla: 1.242 ± 0.031	0.129TrpCys: 0.129 ± 0.01	0.581TrpAsp: 0.581 ± 0.02	0.526TrpGlu: 0.526 ± 0.018	0.509TrpPhe: 0.509 ± 0.016	0.935TrpGly: 0.935 ± 0.03	0.303TrpHis: 0.303 ± 0.013	0.59TrpIle: 0.59 ± 0.02	0.424TrpLys: 0.424 ± 0.017	1.708TrpLeu: 1.708 ± 0.039	0.308TrpMet: 0.308 ± 0.016	0.423TrpAsn: 0.423 ± 0.017	0.712TrpPro: 0.712 ± 0.024	0.555TrpGln: 0.555 ± 0.019	1.19TrpArg: 1.19 ± 0.029	0.846TrpSer: 0.846 ± 0.02	0.766TrpThr: 0.766 ± 0.024	0.716TrpVal: 0.716 ± 0.019	0.227TrpTrp: 0.227 ± 0.012	0.255TrpTyr: 0.255 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.501TyrAla: 2.501 ± 0.044	0.197TyrCys: 0.197 ± 0.011	1.325TyrAsp: 1.325 ± 0.033	1.112TyrGlu: 1.112 ± 0.027	0.771TyrPhe: 0.771 ± 0.025	2.093TyrGly: 2.093 ± 0.042	0.4TyrHis: 0.4 ± 0.017	0.749TyrIle: 0.749 ± 0.023	0.523TyrLys: 0.523 ± 0.02	2.077TyrLeu: 2.077 ± 0.034	0.357TyrMet: 0.357 ± 0.015	0.51TyrAsn: 0.51 ± 0.02	1.09TyrPro: 1.09 ± 0.028	0.642TyrGln: 0.642 ± 0.02	1.738TyrArg: 1.738 ± 0.037	0.997TyrSer: 0.997 ± 0.025	0.964TyrThr: 0.964 ± 0.026	1.506TyrVal: 1.506 ± 0.035	0.351TyrTrp: 0.351 ± 0.016	0.512TyrTyr: 0.512 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5096 proteins (1611244 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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