Wuhan insect virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKE4|A0A1L3KKE4_9VIRU Helicase OS=Wuhan insect virus 9 OX=1923740 PE=4 SV=1
MM1 pKa = 7.91CYY3 pKa = 10.29FILILYY9 pKa = 7.35LTHH12 pKa = 7.05IAHH15 pKa = 7.28GAPVNTKK22 pKa = 7.17TTTLRR27 pKa = 11.84NLNITFPGIFQHH39 pKa = 6.55NFLDD43 pKa = 4.62TIDD46 pKa = 5.25FYY48 pKa = 11.18PSNKK52 pKa = 7.49YY53 pKa = 6.98TQFIAPKK60 pKa = 8.55FQSKK64 pKa = 10.08CMYY67 pKa = 10.64ASVEE71 pKa = 4.04NFLTEE76 pKa = 4.05SNYY79 pKa = 10.19VMPQEE84 pKa = 4.89CGLVATMTIHH94 pKa = 8.0DD95 pKa = 4.17SMFTKK100 pKa = 10.53SIVDD104 pKa = 3.45VGTFACTQYY113 pKa = 10.78HH114 pKa = 6.16RR115 pKa = 11.84NKK117 pKa = 10.03CNPPMHH123 pKa = 7.39LYY125 pKa = 8.62TLNYY129 pKa = 9.61TEE131 pKa = 5.13DD132 pKa = 3.7AFVNDD137 pKa = 4.12IITYY141 pKa = 8.16YY142 pKa = 10.64SKK144 pKa = 9.87EE145 pKa = 4.44TITFRR150 pKa = 11.84YY151 pKa = 7.84TLDD154 pKa = 3.29TTLINYY160 pKa = 9.63IIVLKK165 pKa = 10.82DD166 pKa = 3.17EE167 pKa = 4.38YY168 pKa = 11.03TGMFYY173 pKa = 10.17WITSYY178 pKa = 10.62CLKK181 pKa = 10.73YY182 pKa = 8.34MTVTSDD188 pKa = 2.87NKK190 pKa = 10.0FVHH193 pKa = 6.82LGPGIHH199 pKa = 7.01HH200 pKa = 6.93YY201 pKa = 10.6DD202 pKa = 3.19AATKK206 pKa = 8.94QFCITDD212 pKa = 3.63YY213 pKa = 11.73SNMCTPVGPFKK224 pKa = 10.78TKK226 pKa = 9.78IDD228 pKa = 4.57FILSLPLEE236 pKa = 4.25ITISSDD242 pKa = 3.2RR243 pKa = 11.84PYY245 pKa = 10.59HH246 pKa = 6.79KK247 pKa = 10.59YY248 pKa = 10.34FEE250 pKa = 4.22KK251 pKa = 9.63HH252 pKa = 5.74TYY254 pKa = 10.73DD255 pKa = 3.42DD256 pKa = 4.38TVKK259 pKa = 9.48HH260 pKa = 6.22TIPYY264 pKa = 9.87DD265 pKa = 3.59DD266 pKa = 3.83NNRR269 pKa = 11.84FEE271 pKa = 4.54FDD273 pKa = 3.55KK274 pKa = 11.04NDD276 pKa = 3.26HH277 pKa = 5.8FVYY280 pKa = 10.52VAGNIRR286 pKa = 11.84SMQPNYY292 pKa = 10.5QCVKK296 pKa = 7.79YY297 pKa = 9.74HH298 pKa = 5.35YY299 pKa = 10.38VPMSSIFHH307 pKa = 6.75IDD309 pKa = 3.53IIIDD313 pKa = 3.95SIEE316 pKa = 4.22KK317 pKa = 10.66KK318 pKa = 6.87MTQYY322 pKa = 11.06FHH324 pKa = 7.45LLTNFINDD332 pKa = 3.22NVHH335 pKa = 6.73IIVEE339 pKa = 4.43HH340 pKa = 6.57IEE342 pKa = 4.22NIVLVTCSYY351 pKa = 10.02VVKK354 pKa = 10.56KK355 pKa = 10.17ILYY358 pKa = 9.71LITRR362 pKa = 11.84ILAYY366 pKa = 10.13SYY368 pKa = 10.96IMEE371 pKa = 4.65AFAITMYY378 pKa = 10.87MMITNYY384 pKa = 10.51NYY386 pKa = 7.72TTIACVVATFCILKK400 pKa = 9.84KK401 pKa = 9.9WLFMWW406 pKa = 4.73

Molecular weight: 47.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KKB7|A0A1L3KKB7_9VIRU Uncharacterized protein OS=Wuhan insect virus 9 OX=1923740 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.34YY4 pKa = 10.26QKK6 pKa = 9.84TITFVNKK13 pKa = 9.97KK14 pKa = 9.13YY15 pKa = 10.65KK16 pKa = 10.13NRR18 pKa = 11.84NALPMKK24 pKa = 9.47PRR26 pKa = 11.84RR27 pKa = 11.84SRR29 pKa = 11.84SRR31 pKa = 11.84TPSVRR36 pKa = 11.84RR37 pKa = 11.84SRR39 pKa = 11.84SRR41 pKa = 11.84SRR43 pKa = 11.84SRR45 pKa = 11.84TPKK48 pKa = 9.42RR49 pKa = 11.84TTIKK53 pKa = 10.59YY54 pKa = 9.34GVVDD58 pKa = 3.78GFINRR63 pKa = 11.84IMKK66 pKa = 9.93KK67 pKa = 10.06FSSILVDD74 pKa = 4.06PYY76 pKa = 11.2TYY78 pKa = 10.15IGLALMSLLMYY89 pKa = 10.07IHH91 pKa = 6.63YY92 pKa = 9.67FHH94 pKa = 7.68IDD96 pKa = 3.36DD97 pKa = 4.18SAVNSIVKK105 pKa = 8.44TLKK108 pKa = 10.96KK109 pKa = 10.29NDD111 pKa = 3.49STKK114 pKa = 11.11AFADD118 pKa = 3.44WVEE121 pKa = 4.03GNINKK126 pKa = 9.17IFAIVMLLYY135 pKa = 10.5HH136 pKa = 6.7SLQIPIKK143 pKa = 9.33YY144 pKa = 9.87RR145 pKa = 11.84YY146 pKa = 8.07FTILLSMITVFLMPSLSVVTYY167 pKa = 9.13SATIFGVVMYY177 pKa = 10.44HH178 pKa = 6.57KK179 pKa = 9.92MKK181 pKa = 10.63KK182 pKa = 8.88RR183 pKa = 11.84TDD185 pKa = 2.99KK186 pKa = 11.19FIILLFYY193 pKa = 10.19IIIMSWFYY201 pKa = 10.37YY202 pKa = 10.64DD203 pKa = 5.52EE204 pKa = 4.81IIKK207 pKa = 9.88YY208 pKa = 9.48HH209 pKa = 6.28SKK211 pKa = 5.56TTRR214 pKa = 11.84VKK216 pKa = 10.83RR217 pKa = 11.84EE218 pKa = 3.85TEE220 pKa = 3.96EE221 pKa = 3.92QQVV224 pKa = 3.07

Molecular weight: 26.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3048

224

2418

1016.0

117.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.199 ± 0.364

1.739 ± 0.442

7.283 ± 1.287

4.199 ± 0.684

4.79 ± 0.674

3.051 ± 0.399

2.953 ± 0.437

8.071 ± 1.239

8.399 ± 0.742

7.415 ± 0.361

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.707 ± 0.387

5.381 ± 0.407

3.051 ± 0.287

2.329 ± 0.152

3.675 ± 0.899

7.907 ± 0.969

7.283 ± 1.108

7.087 ± 0.376

0.623 ± 0.089

6.857 ± 0.606
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski