Hazenella coriacea
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2870 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3L6K5|A0A4R3L6K5_9BACL tRNA (guanine-N(7)-)-methyltransferase OS=Hazenella coriacea OX=1179467 GN=trmB PE=3 SV=1
MM1 pKa = 7.62 LIIFQFQDD9 pKa = 2.69 GCNWYY14 pKa = 10.02 DD15 pKa = 3.37 EE16 pKa = 4.35 YY17 pKa = 11.51 MNDD20 pKa = 5.36 LLPQSIIIGDD30 pKa = 3.7 DD31 pKa = 3.33 SGSGKK36 pKa = 9.82 IVLVVDD42 pKa = 3.83 PEE44 pKa = 4.51 YY45 pKa = 11.09 KK46 pKa = 10.18 GVYY49 pKa = 8.73 YY50 pKa = 9.86 WDD52 pKa = 3.23 HH53 pKa = 6.54 TYY55 pKa = 11.4 DD56 pKa = 4.31 FEE58 pKa = 4.76 QLSDD62 pKa = 3.7 EE63 pKa = 4.28 EE64 pKa = 4.31 NTYY67 pKa = 10.77 KK68 pKa = 10.47 IADD71 pKa = 3.65 SFQRR75 pKa = 11.84 FIDD78 pKa = 3.98 GLKK81 pKa = 10.66 NPP83 pKa = 4.71
Molecular weight: 9.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 0.54
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A4R3L6L7|A0A4R3L6L7_9BACL Proline iminopeptidase OS=Hazenella coriacea OX=1179467 GN=EDD58_10552 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.7 NVHH16 pKa = 5.13 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 9.18 NGRR28 pKa = 11.84 RR29 pKa = 11.84 ILSARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.85 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2870
0
2870
845007
30
5540
294.4
33.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.459 ± 0.042
0.834 ± 0.014
4.899 ± 0.033
7.327 ± 0.056
4.228 ± 0.034
6.78 ± 0.044
2.429 ± 0.026
7.452 ± 0.044
6.247 ± 0.043
10.205 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.732 ± 0.019
3.602 ± 0.031
4.077 ± 0.027
4.958 ± 0.037
4.723 ± 0.038
6.127 ± 0.039
5.209 ± 0.027
7.101 ± 0.036
1.288 ± 0.022
3.323 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here