Gordonia phage Ohgeesy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; Baxtervirus; unclassified Baxtervirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A7G8LG86|A0A7G8LG86_9CAUD Uncharacterized protein OS=Gordonia phage Ohgeesy OX=2762412 GN=29 PE=4 SV=1
MM1 pKa = 7.17STPLVPDD8 pKa = 3.18WTDD11 pKa = 2.91SDD13 pKa = 4.06IEE15 pKa = 4.34IEE17 pKa = 4.33VPDD20 pKa = 4.07NQVQVIDD27 pKa = 4.08APAVEE32 pKa = 4.43DD33 pKa = 3.94VEE35 pKa = 4.62VLVIQGRR42 pKa = 11.84DD43 pKa = 3.71GQGLQPDD50 pKa = 4.17AAVDD54 pKa = 4.21TYY56 pKa = 12.04ADD58 pKa = 4.33LPDD61 pKa = 4.88DD62 pKa = 5.43LGPNDD67 pKa = 4.05SGQVVYY73 pKa = 10.71VWSDD77 pKa = 2.91KK78 pKa = 11.07LIYY81 pKa = 9.62IWSGTDD87 pKa = 3.02WPAEE91 pKa = 3.95GAGKK95 pKa = 9.87DD96 pKa = 3.4IRR98 pKa = 11.84GMQGDD103 pKa = 4.39PGRR106 pKa = 11.84GIDD109 pKa = 3.62DD110 pKa = 3.73VFVVGTSLVFSMSDD124 pKa = 3.14NTSDD128 pKa = 4.28DD129 pKa = 3.82VPVPAIQQAIDD140 pKa = 3.28SAAAASGSATAADD153 pKa = 4.08TARR156 pKa = 11.84LAAEE160 pKa = 4.66AAASTAGTAASTATTEE176 pKa = 3.77RR177 pKa = 11.84TAAQTARR184 pKa = 11.84TAAEE188 pKa = 4.14AARR191 pKa = 11.84DD192 pKa = 3.65TATNRR197 pKa = 11.84ATAASNSADD206 pKa = 3.11AAATSEE212 pKa = 4.26ANAEE216 pKa = 4.11TSEE219 pKa = 4.54DD220 pKa = 3.61NAATSAAAAATSAGQAANRR239 pKa = 11.84AADD242 pKa = 3.78ADD244 pKa = 4.11TARR247 pKa = 11.84AAAVLARR254 pKa = 11.84DD255 pKa = 3.72AAAGSATDD263 pKa = 3.64AATSAGSAQTSADD276 pKa = 3.72DD277 pKa = 4.36AGTSATAAAASAEE290 pKa = 4.12EE291 pKa = 4.16AADD294 pKa = 3.74VVAAGVPDD302 pKa = 3.69ATDD305 pKa = 3.36TTKK308 pKa = 11.19GGIILAGDD316 pKa = 4.33LGGTWDD322 pKa = 4.35EE323 pKa = 4.25PTVPALAMKK332 pKa = 10.57ADD334 pKa = 4.18LDD336 pKa = 4.64SNGKK340 pKa = 9.07LLSSQMPAQATHH352 pKa = 6.33EE353 pKa = 4.64SVVVTSTAEE362 pKa = 3.9RR363 pKa = 11.84LALTPAQVQPGDD375 pKa = 3.47TAIQLGNPGRR385 pKa = 11.84GTYY388 pKa = 10.03SLQGADD394 pKa = 3.84PSDD397 pKa = 3.83PGSWVLQVAPTDD409 pKa = 3.58AVSSVNGYY417 pKa = 9.61QGIVVLGKK425 pKa = 10.87GDD427 pKa = 3.53VGLGNVDD434 pKa = 3.46NTSDD438 pKa = 3.59LAKK441 pKa = 10.4PISNDD446 pKa = 2.68VAAALAGKK454 pKa = 9.89VDD456 pKa = 4.16EE457 pKa = 4.59VSTANVAYY465 pKa = 7.43GTKK468 pKa = 9.61TGGVQGTWPVTSAATATTLALRR490 pKa = 11.84GTGGTVAVGTATGPTHH506 pKa = 7.37APTKK510 pKa = 9.82QQLDD514 pKa = 3.72DD515 pKa = 4.24GLSGKK520 pKa = 10.37SDD522 pKa = 3.2TGHH525 pKa = 6.61AHH527 pKa = 6.98DD528 pKa = 5.03AAAIATGTLDD538 pKa = 3.78AARR541 pKa = 11.84LPVGTGSTQVAAGNDD556 pKa = 3.49SRR558 pKa = 11.84IVNAVPNSRR567 pKa = 11.84TVTAGTGLSGGGTLDD582 pKa = 3.23VNRR585 pKa = 11.84TLSVLYY591 pKa = 10.92GNTANTAAQGNDD603 pKa = 2.96LRR605 pKa = 11.84LSDD608 pKa = 4.05TRR610 pKa = 11.84TPTDD614 pKa = 3.41NTVSTAKK621 pKa = 10.49LQDD624 pKa = 3.56GAVTLAKK631 pKa = 10.32LATAVSVSIQQMIDD645 pKa = 3.32VSVLAAQLVTINAQTGAYY663 pKa = 8.53TLVATDD669 pKa = 3.4ANKK672 pKa = 10.44AVEE675 pKa = 4.47VTSGSAVNVTIPTNASVAFPIGTVIEE701 pKa = 4.13VDD703 pKa = 3.11QMGAGKK709 pKa = 8.3VTIVGASGVTVQSAVATPTSRR730 pKa = 11.84AQYY733 pKa = 9.71SALVLRR739 pKa = 11.84KK740 pKa = 9.36RR741 pKa = 11.84AADD744 pKa = 3.35LWLVTGDD751 pKa = 3.86LAA753 pKa = 6.13

Molecular weight:
74.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A7G8LGE2|A0A7G8LGE2_9CAUD HNH endonuclease OS=Gordonia phage Ohgeesy OX=2762412 GN=85 PE=4 SV=1
MM1 pKa = 7.25PTAKK5 pKa = 9.64QRR7 pKa = 11.84NTTQRR12 pKa = 11.84GLGWSHH18 pKa = 5.24QKK20 pKa = 9.88QRR22 pKa = 11.84VRR24 pKa = 11.84LLGKK28 pKa = 8.53HH29 pKa = 6.03TDD31 pKa = 3.51TTPCWWCGEE40 pKa = 3.85PMYY43 pKa = 10.47RR44 pKa = 11.84DD45 pKa = 3.77PKK47 pKa = 10.58RR48 pKa = 11.84NPDD51 pKa = 3.58YY52 pKa = 11.28DD53 pKa = 3.84PASNDD58 pKa = 3.67PASGALAADD67 pKa = 3.98HH68 pKa = 6.03TRR70 pKa = 11.84SRR72 pKa = 11.84ATGGTVADD80 pKa = 4.41RR81 pKa = 11.84LLHH84 pKa = 5.56GLCNKK89 pKa = 9.44QRR91 pKa = 11.84GDD93 pKa = 3.34GSRR96 pKa = 11.84DD97 pKa = 3.25HH98 pKa = 6.57QRR100 pKa = 11.84PALLKK105 pKa = 10.54RR106 pKa = 11.84RR107 pKa = 11.84GGHH110 pKa = 6.49PANSLNWGG118 pKa = 3.56

Molecular weight:
13.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17374

41

2139

204.4

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.127 ± 0.624

0.863 ± 0.16

7.218 ± 0.42

5.836 ± 0.264

2.59 ± 0.178

8.196 ± 0.359

1.928 ± 0.199

4.461 ± 0.153

3.269 ± 0.216

7.851 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.951 ± 0.186

3.016 ± 0.191

5.6 ± 0.251

3.465 ± 0.289

7.931 ± 0.481

5.583 ± 0.22

6.849 ± 0.322

7.166 ± 0.208

2.13 ± 0.198

1.968 ± 0.124

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski