Gordonia phage Ohgeesy
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G8LG86|A0A7G8LG86_9CAUD Uncharacterized protein OS=Gordonia phage Ohgeesy OX=2762412 GN=29 PE=4 SV=1
MM1 pKa = 7.17 STPLVPDD8 pKa = 3.18 WTDD11 pKa = 2.91 SDD13 pKa = 4.06 IEE15 pKa = 4.34 IEE17 pKa = 4.33 VPDD20 pKa = 4.07 NQVQVIDD27 pKa = 4.08 APAVEE32 pKa = 4.43 DD33 pKa = 3.94 VEE35 pKa = 4.62 VLVIQGRR42 pKa = 11.84 DD43 pKa = 3.71 GQGLQPDD50 pKa = 4.17 AAVDD54 pKa = 4.21 TYY56 pKa = 12.04 ADD58 pKa = 4.33 LPDD61 pKa = 4.88 DD62 pKa = 5.43 LGPNDD67 pKa = 4.05 SGQVVYY73 pKa = 10.71 VWSDD77 pKa = 2.91 KK78 pKa = 11.07 LIYY81 pKa = 9.62 IWSGTDD87 pKa = 3.02 WPAEE91 pKa = 3.95 GAGKK95 pKa = 9.87 DD96 pKa = 3.4 IRR98 pKa = 11.84 GMQGDD103 pKa = 4.39 PGRR106 pKa = 11.84 GIDD109 pKa = 3.62 DD110 pKa = 3.73 VFVVGTSLVFSMSDD124 pKa = 3.14 NTSDD128 pKa = 4.28 DD129 pKa = 3.82 VPVPAIQQAIDD140 pKa = 3.28 SAAAASGSATAADD153 pKa = 4.08 TARR156 pKa = 11.84 LAAEE160 pKa = 4.66 AAASTAGTAASTATTEE176 pKa = 3.77 RR177 pKa = 11.84 TAAQTARR184 pKa = 11.84 TAAEE188 pKa = 4.14 AARR191 pKa = 11.84 DD192 pKa = 3.65 TATNRR197 pKa = 11.84 ATAASNSADD206 pKa = 3.11 AAATSEE212 pKa = 4.26 ANAEE216 pKa = 4.11 TSEE219 pKa = 4.54 DD220 pKa = 3.61 NAATSAAAAATSAGQAANRR239 pKa = 11.84 AADD242 pKa = 3.78 ADD244 pKa = 4.11 TARR247 pKa = 11.84 AAAVLARR254 pKa = 11.84 DD255 pKa = 3.72 AAAGSATDD263 pKa = 3.64 AATSAGSAQTSADD276 pKa = 3.72 DD277 pKa = 4.36 AGTSATAAAASAEE290 pKa = 4.12 EE291 pKa = 4.16 AADD294 pKa = 3.74 VVAAGVPDD302 pKa = 3.69 ATDD305 pKa = 3.36 TTKK308 pKa = 11.19 GGIILAGDD316 pKa = 4.33 LGGTWDD322 pKa = 4.35 EE323 pKa = 4.25 PTVPALAMKK332 pKa = 10.57 ADD334 pKa = 4.18 LDD336 pKa = 4.64 SNGKK340 pKa = 9.07 LLSSQMPAQATHH352 pKa = 6.33 EE353 pKa = 4.64 SVVVTSTAEE362 pKa = 3.9 RR363 pKa = 11.84 LALTPAQVQPGDD375 pKa = 3.47 TAIQLGNPGRR385 pKa = 11.84 GTYY388 pKa = 10.03 SLQGADD394 pKa = 3.84 PSDD397 pKa = 3.83 PGSWVLQVAPTDD409 pKa = 3.58 AVSSVNGYY417 pKa = 9.61 QGIVVLGKK425 pKa = 10.87 GDD427 pKa = 3.53 VGLGNVDD434 pKa = 3.46 NTSDD438 pKa = 3.59 LAKK441 pKa = 10.4 PISNDD446 pKa = 2.68 VAAALAGKK454 pKa = 9.89 VDD456 pKa = 4.16 EE457 pKa = 4.59 VSTANVAYY465 pKa = 7.43 GTKK468 pKa = 9.61 TGGVQGTWPVTSAATATTLALRR490 pKa = 11.84 GTGGTVAVGTATGPTHH506 pKa = 7.37 APTKK510 pKa = 9.82 QQLDD514 pKa = 3.72 DD515 pKa = 4.24 GLSGKK520 pKa = 10.37 SDD522 pKa = 3.2 TGHH525 pKa = 6.61 AHH527 pKa = 6.98 DD528 pKa = 5.03 AAAIATGTLDD538 pKa = 3.78 AARR541 pKa = 11.84 LPVGTGSTQVAAGNDD556 pKa = 3.49 SRR558 pKa = 11.84 IVNAVPNSRR567 pKa = 11.84 TVTAGTGLSGGGTLDD582 pKa = 3.23 VNRR585 pKa = 11.84 TLSVLYY591 pKa = 10.92 GNTANTAAQGNDD603 pKa = 2.96 LRR605 pKa = 11.84 LSDD608 pKa = 4.05 TRR610 pKa = 11.84 TPTDD614 pKa = 3.41 NTVSTAKK621 pKa = 10.49 LQDD624 pKa = 3.56 GAVTLAKK631 pKa = 10.32 LATAVSVSIQQMIDD645 pKa = 3.32 VSVLAAQLVTINAQTGAYY663 pKa = 8.53 TLVATDD669 pKa = 3.4 ANKK672 pKa = 10.44 AVEE675 pKa = 4.47 VTSGSAVNVTIPTNASVAFPIGTVIEE701 pKa = 4.13 VDD703 pKa = 3.11 QMGAGKK709 pKa = 8.3 VTIVGASGVTVQSAVATPTSRR730 pKa = 11.84 AQYY733 pKa = 9.71 SALVLRR739 pKa = 11.84 KK740 pKa = 9.36 RR741 pKa = 11.84 AADD744 pKa = 3.35 LWLVTGDD751 pKa = 3.86 LAA753 pKa = 6.13
Molecular weight: 74.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.33
IPC2_protein 3.961
IPC_protein 4.012
Toseland 3.77
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.961
Rodwell 3.821
Grimsley 3.668
Solomon 4.012
Lehninger 3.961
Nozaki 4.113
DTASelect 4.418
Thurlkill 3.821
EMBOSS 3.973
Sillero 4.126
Patrickios 3.961
IPC_peptide 3.999
IPC2_peptide 4.101
IPC2.peptide.svr19 4.071
Protein with the highest isoelectric point:
>tr|A0A7G8LGE2|A0A7G8LGE2_9CAUD HNH endonuclease OS=Gordonia phage Ohgeesy OX=2762412 GN=85 PE=4 SV=1
MM1 pKa = 7.25 PTAKK5 pKa = 9.64 QRR7 pKa = 11.84 NTTQRR12 pKa = 11.84 GLGWSHH18 pKa = 5.24 QKK20 pKa = 9.88 QRR22 pKa = 11.84 VRR24 pKa = 11.84 LLGKK28 pKa = 8.53 HH29 pKa = 6.03 TDD31 pKa = 3.51 TTPCWWCGEE40 pKa = 3.85 PMYY43 pKa = 10.47 RR44 pKa = 11.84 DD45 pKa = 3.77 PKK47 pKa = 10.58 RR48 pKa = 11.84 NPDD51 pKa = 3.58 YY52 pKa = 11.28 DD53 pKa = 3.84 PASNDD58 pKa = 3.67 PASGALAADD67 pKa = 3.98 HH68 pKa = 6.03 TRR70 pKa = 11.84 SRR72 pKa = 11.84 ATGGTVADD80 pKa = 4.41 RR81 pKa = 11.84 LLHH84 pKa = 5.56 GLCNKK89 pKa = 9.44 QRR91 pKa = 11.84 GDD93 pKa = 3.34 GSRR96 pKa = 11.84 DD97 pKa = 3.25 HH98 pKa = 6.57 QRR100 pKa = 11.84 PALLKK105 pKa = 10.54 RR106 pKa = 11.84 RR107 pKa = 11.84 GGHH110 pKa = 6.49 PANSLNWGG118 pKa = 3.56
Molecular weight: 13.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.502
IPC_protein 10.116
Toseland 10.628
ProMoST 10.277
Dawson 10.716
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 10.935
Grimsley 10.745
Solomon 10.833
Lehninger 10.804
Nozaki 10.643
DTASelect 10.394
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.643
Patrickios 10.701
IPC_peptide 10.833
IPC2_peptide 9.56
IPC2.peptide.svr19 8.594
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
0
85
17374
41
2139
204.4
22.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.127 ± 0.624
0.863 ± 0.16
7.218 ± 0.42
5.836 ± 0.264
2.59 ± 0.178
8.196 ± 0.359
1.928 ± 0.199
4.461 ± 0.153
3.269 ± 0.216
7.851 ± 0.24
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.951 ± 0.186
3.016 ± 0.191
5.6 ± 0.251
3.465 ± 0.289
7.931 ± 0.481
5.583 ± 0.22
6.849 ± 0.322
7.166 ± 0.208
2.13 ± 0.198
1.968 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here