Amino acid dipepetide frequency for Gordonia phage Ohgeesy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.765AlaAla: 18.765 ± 2.313
1.036AlaCys: 1.036 ± 0.264
8.922AlaAsp: 8.922 ± 0.57
7.771AlaGlu: 7.771 ± 0.81
3.281AlaPhe: 3.281 ± 0.405
8.979AlaGly: 8.979 ± 0.757
1.784AlaHis: 1.784 ± 0.267
5.583AlaIle: 5.583 ± 0.444
4.72AlaLys: 4.72 ± 0.633
9.325AlaLeu: 9.325 ± 0.671
2.763AlaMet: 2.763 ± 0.491
3.396AlaAsn: 3.396 ± 0.363
5.814AlaPro: 5.814 ± 0.535
4.259AlaGln: 4.259 ± 0.754
8.116AlaArg: 8.116 ± 0.781
6.907AlaSer: 6.907 ± 0.938
7.656AlaThr: 7.656 ± 1.27
8.461AlaVal: 8.461 ± 0.832
1.612AlaTrp: 1.612 ± 0.275
2.13AlaTyr: 2.13 ± 0.307
0.0AlaXaa: 0.0 ± 0.0
Cys
0.806CysAla: 0.806 ± 0.285
0.115CysCys: 0.115 ± 0.074
0.806CysAsp: 0.806 ± 0.308
0.576CysGlu: 0.576 ± 0.206
0.173CysPhe: 0.173 ± 0.115
1.209CysGly: 1.209 ± 0.309
0.23CysHis: 0.23 ± 0.11
0.115CysIle: 0.115 ± 0.083
0.288CysLys: 0.288 ± 0.133
0.518CysLeu: 0.518 ± 0.172
0.115CysMet: 0.115 ± 0.086
0.403CysAsn: 0.403 ± 0.157
0.46CysPro: 0.46 ± 0.163
0.058CysGln: 0.058 ± 0.056
0.863CysArg: 0.863 ± 0.3
0.288CysSer: 0.288 ± 0.169
0.46CysThr: 0.46 ± 0.205
0.403CysVal: 0.403 ± 0.146
0.345CysTrp: 0.345 ± 0.14
0.403CysTyr: 0.403 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
7.713AspAla: 7.713 ± 0.84
0.691AspCys: 0.691 ± 0.255
6.447AspAsp: 6.447 ± 0.845
5.641AspGlu: 5.641 ± 0.702
2.015AspPhe: 2.015 ± 0.477
5.065AspGly: 5.065 ± 0.541
1.899AspHis: 1.899 ± 0.368
2.13AspIle: 2.13 ± 0.381
1.957AspLys: 1.957 ± 0.33
7.022AspLeu: 7.022 ± 0.516
1.266AspMet: 1.266 ± 0.328
2.36AspAsn: 2.36 ± 0.382
5.871AspPro: 5.871 ± 0.59
1.784AspGln: 1.784 ± 0.284
5.18AspArg: 5.18 ± 0.77
2.936AspSer: 2.936 ± 0.509
3.914AspThr: 3.914 ± 0.448
5.468AspVal: 5.468 ± 0.547
1.784AspTrp: 1.784 ± 0.321
1.036AspTyr: 1.036 ± 0.221
0.0AspXaa: 0.0 ± 0.0
Glu
7.54GluAla: 7.54 ± 0.829
0.633GluCys: 0.633 ± 0.2
3.051GluAsp: 3.051 ± 0.418
2.878GluGlu: 2.878 ± 0.431
2.36GluPhe: 2.36 ± 0.33
2.82GluGly: 2.82 ± 0.459
1.094GluHis: 1.094 ± 0.22
3.914GluIle: 3.914 ± 0.524
2.302GluLys: 2.302 ± 0.386
5.929GluLeu: 5.929 ± 0.704
0.863GluMet: 0.863 ± 0.223
1.209GluAsn: 1.209 ± 0.313
2.936GluPro: 2.936 ± 0.456
2.475GluGln: 2.475 ± 0.424
5.18GluArg: 5.18 ± 0.575
3.626GluSer: 3.626 ± 0.465
2.936GluThr: 2.936 ± 0.438
3.857GluVal: 3.857 ± 0.495
1.381GluTrp: 1.381 ± 0.27
1.381GluTyr: 1.381 ± 0.233
0.0GluXaa: 0.0 ± 0.0
Phe
2.533PheAla: 2.533 ± 0.382
0.115PheCys: 0.115 ± 0.083
2.705PheAsp: 2.705 ± 0.424
2.072PheGlu: 2.072 ± 0.348
0.46PhePhe: 0.46 ± 0.144
2.533PheGly: 2.533 ± 0.367
0.576PheHis: 0.576 ± 0.202
0.863PheIle: 0.863 ± 0.228
0.633PheLys: 0.633 ± 0.219
1.209PheLeu: 1.209 ± 0.313
0.46PheMet: 0.46 ± 0.134
1.036PheAsn: 1.036 ± 0.232
1.324PhePro: 1.324 ± 0.302
0.46PheGln: 0.46 ± 0.156
2.072PheArg: 2.072 ± 0.419
1.669PheSer: 1.669 ± 0.319
1.612PheThr: 1.612 ± 0.305
1.842PheVal: 1.842 ± 0.381
0.979PheTrp: 0.979 ± 0.208
0.748PheTyr: 0.748 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
7.656GlyAla: 7.656 ± 0.91
0.518GlyCys: 0.518 ± 0.193
5.871GlyAsp: 5.871 ± 0.507
4.202GlyGlu: 4.202 ± 0.583
2.36GlyPhe: 2.36 ± 0.342
8.346GlyGly: 8.346 ± 1.08
1.497GlyHis: 1.497 ± 0.331
3.396GlyIle: 3.396 ± 0.325
3.569GlyLys: 3.569 ± 0.416
7.195GlyLeu: 7.195 ± 0.658
1.957GlyMet: 1.957 ± 0.308
3.108GlyAsn: 3.108 ± 0.469
3.511GlyPro: 3.511 ± 0.494
2.533GlyGln: 2.533 ± 0.446
6.274GlyArg: 6.274 ± 0.644
4.893GlySer: 4.893 ± 0.634
5.641GlyThr: 5.641 ± 1.186
5.929GlyVal: 5.929 ± 0.799
1.612GlyTrp: 1.612 ± 0.342
1.842GlyTyr: 1.842 ± 0.293
0.0GlyXaa: 0.0 ± 0.0
His
2.302HisAla: 2.302 ± 0.324
0.058HisCys: 0.058 ± 0.056
1.497HisAsp: 1.497 ± 0.375
1.439HisGlu: 1.439 ± 0.313
0.46HisPhe: 0.46 ± 0.167
1.036HisGly: 1.036 ± 0.207
0.46HisHis: 0.46 ± 0.168
0.576HisIle: 0.576 ± 0.216
0.288HisLys: 0.288 ± 0.148
1.784HisLeu: 1.784 ± 0.359
0.288HisMet: 0.288 ± 0.123
0.173HisAsn: 0.173 ± 0.098
1.612HisPro: 1.612 ± 0.381
0.748HisGln: 0.748 ± 0.236
1.669HisArg: 1.669 ± 0.319
0.921HisSer: 0.921 ± 0.207
1.439HisThr: 1.439 ± 0.282
1.439HisVal: 1.439 ± 0.31
0.576HisTrp: 0.576 ± 0.166
0.518HisTyr: 0.518 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
4.835IleAla: 4.835 ± 0.479
0.288IleCys: 0.288 ± 0.139
5.468IleAsp: 5.468 ± 0.668
3.626IleGlu: 3.626 ± 0.433
0.46IlePhe: 0.46 ± 0.149
4.087IleGly: 4.087 ± 0.557
0.979IleHis: 0.979 ± 0.232
1.209IleIle: 1.209 ± 0.269
1.266IleLys: 1.266 ± 0.285
2.878IleLeu: 2.878 ± 0.392
0.403IleMet: 0.403 ± 0.134
1.036IleAsn: 1.036 ± 0.262
2.245IlePro: 2.245 ± 0.343
1.324IleGln: 1.324 ± 0.295
3.857IleArg: 3.857 ± 0.467
2.705IleSer: 2.705 ± 0.327
2.418IleThr: 2.418 ± 0.474
4.029IleVal: 4.029 ± 0.574
0.345IleTrp: 0.345 ± 0.131
1.151IleTyr: 1.151 ± 0.278
0.0IleXaa: 0.0 ± 0.0
Lys
4.202LysAla: 4.202 ± 0.781
0.115LysCys: 0.115 ± 0.087
1.266LysAsp: 1.266 ± 0.267
1.842LysGlu: 1.842 ± 0.318
0.979LysPhe: 0.979 ± 0.226
2.13LysGly: 2.13 ± 0.414
0.633LysHis: 0.633 ± 0.182
1.612LysIle: 1.612 ± 0.278
1.209LysLys: 1.209 ± 0.312
2.763LysLeu: 2.763 ± 0.422
0.633LysMet: 0.633 ± 0.233
1.094LysAsn: 1.094 ± 0.194
2.015LysPro: 2.015 ± 0.303
1.094LysGln: 1.094 ± 0.314
2.59LysArg: 2.59 ± 0.409
2.302LysSer: 2.302 ± 0.437
2.13LysThr: 2.13 ± 0.39
2.418LysVal: 2.418 ± 0.283
0.806LysTrp: 0.806 ± 0.264
0.863LysTyr: 0.863 ± 0.174
0.0LysXaa: 0.0 ± 0.0
Leu
10.016LeuAla: 10.016 ± 0.929
0.748LeuCys: 0.748 ± 0.231
6.389LeuAsp: 6.389 ± 0.713
3.972LeuGlu: 3.972 ± 0.461
2.59LeuPhe: 2.59 ± 0.574
6.504LeuGly: 6.504 ± 0.521
1.784LeuHis: 1.784 ± 0.388
4.202LeuIle: 4.202 ± 0.486
2.072LeuLys: 2.072 ± 0.371
5.123LeuLeu: 5.123 ± 0.689
1.381LeuMet: 1.381 ± 0.274
2.245LeuAsn: 2.245 ± 0.388
3.626LeuPro: 3.626 ± 0.503
2.648LeuGln: 2.648 ± 0.348
7.138LeuArg: 7.138 ± 0.811
4.202LeuSer: 4.202 ± 0.521
5.123LeuThr: 5.123 ± 0.567
6.044LeuVal: 6.044 ± 0.735
1.497LeuTrp: 1.497 ± 0.31
1.209LeuTyr: 1.209 ± 0.306
0.0LeuXaa: 0.0 ± 0.0
Met
2.763MetAla: 2.763 ± 0.456
0.345MetCys: 0.345 ± 0.139
0.345MetAsp: 0.345 ± 0.13
0.576MetGlu: 0.576 ± 0.184
0.288MetPhe: 0.288 ± 0.124
1.727MetGly: 1.727 ± 0.391
0.46MetHis: 0.46 ± 0.139
0.748MetIle: 0.748 ± 0.191
0.748MetLys: 0.748 ± 0.233
1.497MetLeu: 1.497 ± 0.238
0.23MetMet: 0.23 ± 0.141
0.46MetAsn: 0.46 ± 0.23
1.266MetPro: 1.266 ± 0.213
0.748MetGln: 0.748 ± 0.214
1.784MetArg: 1.784 ± 0.448
1.151MetSer: 1.151 ± 0.24
2.705MetThr: 2.705 ± 0.382
1.036MetVal: 1.036 ± 0.274
0.403MetTrp: 0.403 ± 0.177
0.23MetTyr: 0.23 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.108AsnAla: 3.108 ± 0.429
0.173AsnCys: 0.173 ± 0.113
2.015AsnAsp: 2.015 ± 0.334
1.439AsnGlu: 1.439 ± 0.283
0.288AsnPhe: 0.288 ± 0.161
2.936AsnGly: 2.936 ± 0.383
0.633AsnHis: 0.633 ± 0.16
1.266AsnIle: 1.266 ± 0.262
1.036AsnLys: 1.036 ± 0.225
2.187AsnLeu: 2.187 ± 0.383
0.46AsnMet: 0.46 ± 0.153
0.748AsnAsn: 0.748 ± 0.217
2.418AsnPro: 2.418 ± 0.346
1.324AsnGln: 1.324 ± 0.305
2.648AsnArg: 2.648 ± 0.394
1.957AsnSer: 1.957 ± 0.281
2.36AsnThr: 2.36 ± 0.54
1.669AsnVal: 1.669 ± 0.31
1.036AsnTrp: 1.036 ± 0.234
0.46AsnTyr: 0.46 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
7.31ProAla: 7.31 ± 0.664
0.576ProCys: 0.576 ± 0.238
4.95ProAsp: 4.95 ± 0.745
3.857ProGlu: 3.857 ± 0.603
1.151ProPhe: 1.151 ± 0.259
5.411ProGly: 5.411 ± 0.618
0.691ProHis: 0.691 ± 0.218
2.015ProIle: 2.015 ± 0.324
2.072ProLys: 2.072 ± 0.316
3.339ProLeu: 3.339 ± 0.358
1.266ProMet: 1.266 ± 0.222
2.015ProAsn: 2.015 ± 0.37
2.59ProPro: 2.59 ± 0.502
1.727ProGln: 1.727 ± 0.315
4.317ProArg: 4.317 ± 0.616
3.166ProSer: 3.166 ± 0.4
3.684ProThr: 3.684 ± 0.476
3.684ProVal: 3.684 ± 0.392
1.266ProTrp: 1.266 ± 0.25
0.921ProTyr: 0.921 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
4.202GlnAla: 4.202 ± 0.525
0.23GlnCys: 0.23 ± 0.113
1.497GlnAsp: 1.497 ± 0.276
1.324GlnGlu: 1.324 ± 0.328
0.806GlnPhe: 0.806 ± 0.195
2.475GlnGly: 2.475 ± 0.648
0.921GlnHis: 0.921 ± 0.24
2.187GlnIle: 2.187 ± 0.402
0.921GlnLys: 0.921 ± 0.27
3.799GlnLeu: 3.799 ± 0.451
1.266GlnMet: 1.266 ± 0.244
1.036GlnAsn: 1.036 ± 0.228
1.554GlnPro: 1.554 ± 0.232
1.899GlnGln: 1.899 ± 0.315
2.763GlnArg: 2.763 ± 0.457
1.612GlnSer: 1.612 ± 0.338
1.842GlnThr: 1.842 ± 0.395
2.763GlnVal: 2.763 ± 0.379
1.036GlnTrp: 1.036 ± 0.242
0.518GlnTyr: 0.518 ± 0.182
0.0GlnXaa: 0.0 ± 0.0
Arg
8.289ArgAla: 8.289 ± 0.773
1.151ArgCys: 1.151 ± 0.448
5.18ArgAsp: 5.18 ± 0.684
4.835ArgGlu: 4.835 ± 0.573
1.669ArgPhe: 1.669 ± 0.255
5.583ArgGly: 5.583 ± 0.569
1.497ArgHis: 1.497 ± 0.411
3.914ArgIle: 3.914 ± 0.518
2.878ArgLys: 2.878 ± 0.343
5.871ArgLeu: 5.871 ± 0.565
1.784ArgMet: 1.784 ± 0.289
2.475ArgAsn: 2.475 ± 0.385
4.605ArgPro: 4.605 ± 0.691
3.051ArgGln: 3.051 ± 0.428
7.31ArgArg: 7.31 ± 0.907
4.087ArgSer: 4.087 ± 0.461
5.583ArgThr: 5.583 ± 0.652
5.986ArgVal: 5.986 ± 0.695
1.842ArgTrp: 1.842 ± 0.32
1.727ArgTyr: 1.727 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
6.332SerAla: 6.332 ± 0.84
0.23SerCys: 0.23 ± 0.108
4.087SerAsp: 4.087 ± 0.534
3.223SerGlu: 3.223 ± 0.322
1.324SerPhe: 1.324 ± 0.246
5.986SerGly: 5.986 ± 0.88
0.691SerHis: 0.691 ± 0.182
2.705SerIle: 2.705 ± 0.422
1.497SerLys: 1.497 ± 0.223
3.799SerLeu: 3.799 ± 0.469
1.439SerMet: 1.439 ± 0.439
2.59SerAsn: 2.59 ± 0.401
2.705SerPro: 2.705 ± 0.404
2.015SerGln: 2.015 ± 0.43
3.511SerArg: 3.511 ± 0.447
2.59SerSer: 2.59 ± 0.35
3.914SerThr: 3.914 ± 0.555
4.317SerVal: 4.317 ± 0.536
1.669SerTrp: 1.669 ± 0.291
1.209SerTyr: 1.209 ± 0.332
0.0SerXaa: 0.0 ± 0.0
Thr
10.188ThrAla: 10.188 ± 1.349
0.46ThrCys: 0.46 ± 0.172
4.144ThrAsp: 4.144 ± 0.49
3.684ThrGlu: 3.684 ± 0.495
1.612ThrPhe: 1.612 ± 0.366
5.986ThrGly: 5.986 ± 0.781
1.094ThrHis: 1.094 ± 0.267
3.223ThrIle: 3.223 ± 0.49
1.612ThrLys: 1.612 ± 0.259
4.432ThrLeu: 4.432 ± 0.466
1.209ThrMet: 1.209 ± 0.254
1.842ThrAsn: 1.842 ± 0.282
4.144ThrPro: 4.144 ± 0.526
1.612ThrGln: 1.612 ± 0.295
4.778ThrArg: 4.778 ± 0.633
4.605ThrSer: 4.605 ± 0.706
6.217ThrThr: 6.217 ± 0.616
5.238ThrVal: 5.238 ± 0.522
1.324ThrTrp: 1.324 ± 0.311
1.094ThrTyr: 1.094 ± 0.282
0.0ThrXaa: 0.0 ± 0.0
Val
8.404ValAla: 8.404 ± 0.75
0.576ValCys: 0.576 ± 0.202
5.468ValAsp: 5.468 ± 0.562
3.741ValGlu: 3.741 ± 0.435
2.302ValPhe: 2.302 ± 0.365
4.95ValGly: 4.95 ± 0.662
1.324ValHis: 1.324 ± 0.235
3.972ValIle: 3.972 ± 0.451
2.245ValLys: 2.245 ± 0.351
6.389ValLeu: 6.389 ± 0.824
0.921ValMet: 0.921 ± 0.2
2.245ValAsn: 2.245 ± 0.394
4.778ValPro: 4.778 ± 0.512
3.166ValGln: 3.166 ± 0.709
5.008ValArg: 5.008 ± 0.478
3.684ValSer: 3.684 ± 0.54
5.756ValThr: 5.756 ± 0.613
4.662ValVal: 4.662 ± 0.622
0.979ValTrp: 0.979 ± 0.227
1.094ValTyr: 1.094 ± 0.188
0.0ValXaa: 0.0 ± 0.0
Trp
1.899TrpAla: 1.899 ± 0.359
0.345TrpCys: 0.345 ± 0.142
0.921TrpAsp: 0.921 ± 0.247
1.036TrpGlu: 1.036 ± 0.256
0.806TrpPhe: 0.806 ± 0.246
1.784TrpGly: 1.784 ± 0.298
0.403TrpHis: 0.403 ± 0.171
0.403TrpIle: 0.403 ± 0.151
0.633TrpLys: 0.633 ± 0.195
1.784TrpLeu: 1.784 ± 0.375
0.518TrpMet: 0.518 ± 0.196
0.46TrpAsn: 0.46 ± 0.147
1.324TrpPro: 1.324 ± 0.298
0.863TrpGln: 0.863 ± 0.268
2.878TrpArg: 2.878 ± 0.485
1.094TrpSer: 1.094 ± 0.22
1.727TrpThr: 1.727 ± 0.302
1.324TrpVal: 1.324 ± 0.269
0.518TrpTrp: 0.518 ± 0.163
0.576TrpTyr: 0.576 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.418TyrAla: 2.418 ± 0.309
0.23TyrCys: 0.23 ± 0.119
1.151TyrAsp: 1.151 ± 0.271
0.633TyrGlu: 0.633 ± 0.239
0.518TyrPhe: 0.518 ± 0.172
2.418TyrGly: 2.418 ± 0.379
0.633TyrHis: 0.633 ± 0.209
0.576TyrIle: 0.576 ± 0.211
0.748TyrLys: 0.748 ± 0.215
1.669TyrLeu: 1.669 ± 0.336
0.23TyrMet: 0.23 ± 0.12
0.23TyrAsn: 0.23 ± 0.106
1.209TyrPro: 1.209 ± 0.29
0.863TyrGln: 0.863 ± 0.214
1.381TyrArg: 1.381 ± 0.323
1.439TyrSer: 1.439 ± 0.24
1.381TyrThr: 1.381 ± 0.274
1.094TyrVal: 1.094 ± 0.249
0.288TyrTrp: 0.288 ± 0.147
0.576TyrTyr: 0.576 ± 0.158
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (17374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski