Jeotgalibacillus soli
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3938 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2W091|A0A0C2W091_9BACL Dehydrogenase OS=Jeotgalibacillus soli OX=889306 GN=KP78_14890 PE=4 SV=1
MM1 pKa = 7.39 INKK4 pKa = 9.85 AILVLCIVLTGFVVFWGQHH23 pKa = 3.96 SWSKK27 pKa = 8.52 QQEE30 pKa = 4.57 SIHH33 pKa = 5.57 THH35 pKa = 4.63 TATPVSNKK43 pKa = 9.27 EE44 pKa = 4.28 SEE46 pKa = 4.63 DD47 pKa = 3.59 KK48 pKa = 11.26 AADD51 pKa = 3.66 EE52 pKa = 4.49 PKK54 pKa = 10.61 SVNTNPADD62 pKa = 4.23 SEE64 pKa = 4.55 TLSNLISNQPEE75 pKa = 4.06 NVQKK79 pKa = 10.05 MWLEE83 pKa = 3.91 HH84 pKa = 4.91 QQNDD88 pKa = 3.74 RR89 pKa = 11.84 PVDD92 pKa = 3.36 ITFVASGFALLSEE105 pKa = 4.79 EE106 pKa = 3.96 NWTTLLEE113 pKa = 4.64 DD114 pKa = 3.57 EE115 pKa = 4.42 MTAVYY120 pKa = 10.29 EE121 pKa = 5.43 DD122 pKa = 5.0 EE123 pKa = 5.32 DD124 pKa = 3.87 LQLNFTVVEE133 pKa = 4.17 WEE135 pKa = 4.35 GNSVDD140 pKa = 3.21 WLTSIKK146 pKa = 10.65 NSSTDD151 pKa = 3.43 FAEE154 pKa = 4.64 ADD156 pKa = 3.61 LVVYY160 pKa = 7.44 EE161 pKa = 5.1 IPTIYY166 pKa = 10.73 DD167 pKa = 3.29 NGEE170 pKa = 4.02 VEE172 pKa = 5.96 PEE174 pKa = 3.73 DD175 pKa = 3.38 QQINMEE181 pKa = 4.21 EE182 pKa = 4.3 FISFLLEE189 pKa = 4.21 NYY191 pKa = 8.92 PDD193 pKa = 3.17 THH195 pKa = 8.31 LFVQPSQPLYY205 pKa = 10.14 NAAIYY210 pKa = 8.51 PEE212 pKa = 4.44 HH213 pKa = 6.08 VAGVRR218 pKa = 11.84 LIAEE222 pKa = 4.24 QLNIPYY228 pKa = 9.84 LDD230 pKa = 4.61 HH231 pKa = 5.93 WTDD234 pKa = 3.39 WPDD237 pKa = 3.29 SDD239 pKa = 6.0 DD240 pKa = 4.71 PEE242 pKa = 5.5 LEE244 pKa = 4.15 NYY246 pKa = 8.19 LTEE249 pKa = 5.62 DD250 pKa = 3.33 SDD252 pKa = 4.35 PNEE255 pKa = 4.9 LGNSTWGTYY264 pKa = 9.69 LSNYY268 pKa = 6.99 FTGNN272 pKa = 3.0
Molecular weight: 31.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.643
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.656
Grimsley 3.554
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.668
EMBOSS 3.706
Sillero 3.948
Patrickios 1.202
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A0C2W1B7|A0A0C2W1B7_9BACL Methyltransferase FkbM OS=Jeotgalibacillus soli OX=889306 GN=KP78_13910 PE=4 SV=1
MM1 pKa = 7.37 LTTIVLNIRR10 pKa = 11.84 FPRR13 pKa = 11.84 VVLAALVGASLAIAGAAFQGLLKK36 pKa = 10.93 NPLADD41 pKa = 4.52 PYY43 pKa = 10.61 TLGVSSGAAVGAVMTIFLGITIPGLGGFTLPVVSIIAALVTLILVLGFARR93 pKa = 11.84 LVEE96 pKa = 4.24 QSMKK100 pKa = 9.96 MEE102 pKa = 4.74 TIILTGIIFSSFLGSILSLMIALTGEE128 pKa = 4.4 EE129 pKa = 4.2 LRR131 pKa = 11.84 QIITWLLGSVSMRR144 pKa = 11.84 GWEE147 pKa = 4.5 YY148 pKa = 10.69 IRR150 pKa = 11.84 LIAPFFIFGSAILMWNARR168 pKa = 11.84 EE169 pKa = 3.98 LNAMAFGEE177 pKa = 4.42 EE178 pKa = 4.23 RR179 pKa = 11.84 AHH181 pKa = 6.35 HH182 pKa = 6.32 LGVNVQARR190 pKa = 11.84 KK191 pKa = 9.44 YY192 pKa = 8.17 WVLASGSLLTGAAVAVSGTIGFIGLVVPHH221 pKa = 5.94 MVRR224 pKa = 11.84 RR225 pKa = 11.84 AWGSDD230 pKa = 2.9 HH231 pKa = 6.88 HH232 pKa = 6.98 HH233 pKa = 6.44 VLPLSMINGASLLIICDD250 pKa = 3.94 LVARR254 pKa = 11.84 TIVSPTEE261 pKa = 3.77 LPIGVITALLGAPVFGLILMRR282 pKa = 11.84 QRR284 pKa = 11.84 RR285 pKa = 11.84 EE286 pKa = 3.64 RR287 pKa = 11.84 RR288 pKa = 11.84 GG289 pKa = 3.23
Molecular weight: 30.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.545
IPC_protein 10.628
Toseland 10.57
ProMoST 10.452
Dawson 10.687
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.672
Grimsley 10.76
Solomon 10.862
Lehninger 10.818
Nozaki 10.57
DTASelect 10.482
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.628
Patrickios 10.423
IPC_peptide 10.862
IPC2_peptide 9.648
IPC2.peptide.svr19 8.641
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3938
0
3938
1042058
37
1546
264.6
29.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.282 ± 0.044
0.66 ± 0.011
5.123 ± 0.032
7.65 ± 0.04
4.469 ± 0.032
6.978 ± 0.035
2.193 ± 0.021
7.856 ± 0.041
6.262 ± 0.036
9.744 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.922 ± 0.019
4.204 ± 0.028
3.679 ± 0.021
3.866 ± 0.028
4.25 ± 0.03
6.193 ± 0.03
5.338 ± 0.026
7.015 ± 0.035
1.038 ± 0.014
3.28 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here