Pseudaestuariivita atlantica
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3978 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L1JJ78|A0A0L1JJ78_9RHOB Antirestriction protein OS=Pseudaestuariivita atlantica OX=1317121 GN=ATO11_20670 PE=4 SV=1
MM1 pKa = 7.79 AFRR4 pKa = 11.84 VWFFVLVGLVFWVPRR19 pKa = 11.84 AMAFDD24 pKa = 3.7 VTPTFTPTLLQPTQTTRR41 pKa = 11.84 LRR43 pKa = 11.84 ISFLNSVTSPVTGLTLTDD61 pKa = 3.6 TLPAGMFVATPASAVSSCGGSVSTSNGATEE91 pKa = 4.2 GTITLTGGTIPAQVGDD107 pKa = 4.01 TAGTCYY113 pKa = 10.31 IEE115 pKa = 4.3 VSVYY119 pKa = 10.02 AASSGTYY126 pKa = 9.72 INEE129 pKa = 3.82 VPANDD134 pKa = 3.4 ATGTVAGAPTSNPNPGSATFAATIQNISASMSLDD168 pKa = 2.79 IGGNTLQGGEE178 pKa = 4.09 TANRR182 pKa = 11.84 IITLTNPNDD191 pKa = 3.5 VPLTGVSFVHH201 pKa = 7.01 DD202 pKa = 4.49 LNGDD206 pKa = 3.49 SGFNLNANGPVSGTCGGTGTISDD229 pKa = 4.11 TTPATSDD236 pKa = 3.23 FDD238 pKa = 3.94 TTSVITISDD247 pKa = 3.28 ATIPANGQCTIIYY260 pKa = 9.26 QVEE263 pKa = 4.21 PARR266 pKa = 11.84 DD267 pKa = 3.43 MTRR270 pKa = 11.84 TFNDD274 pKa = 2.99 TTMTHH279 pKa = 6.3 QINGGAITTNEE290 pKa = 4.07 GATNPTFNDD299 pKa = 4.53 PIRR302 pKa = 11.84 TVSGIEE308 pKa = 3.66 VDD310 pKa = 3.49 KK311 pKa = 11.31 TFDD314 pKa = 3.38 GVKK317 pKa = 10.21 NAEE320 pKa = 4.47 LNTNTASGADD330 pKa = 3.67 LGIEE334 pKa = 3.74 ITNYY338 pKa = 9.9 NAAPLPGFTLTDD350 pKa = 3.38 TMPAGIVSTNVFRR363 pKa = 11.84 NTCGGTSSINGGAEE377 pKa = 3.93 VTVTGASLGAASGAPIPGLQVQSCNIDD404 pKa = 3.15 ARR406 pKa = 11.84 VEE408 pKa = 3.91 PSVTGNNTYY417 pKa = 10.64 TNVIAAGDD425 pKa = 3.66 INGFSHH431 pKa = 7.0 TATSANLEE439 pKa = 4.12 VTDD442 pKa = 4.45 YY443 pKa = 10.93 IGPEE447 pKa = 4.17 DD448 pKa = 4.09 PLSVTKK454 pKa = 10.47 AFQMGNIYY462 pKa = 10.44 PGDD465 pKa = 3.86 TQTLTFTLTNTYY477 pKa = 10.71 DD478 pKa = 3.64 EE479 pKa = 4.58 NLLNVSLTDD488 pKa = 3.41 HH489 pKa = 6.74 LVNNTSANAGLRR501 pKa = 11.84 IGAGGVTSNTCQGSASATPNGDD523 pKa = 4.16 LISISGVYY531 pKa = 10.16 LPIGASCQVVVEE543 pKa = 4.12 VTMASDD549 pKa = 3.58 AQNSRR554 pKa = 11.84 TIWNTVPQSQITFDD568 pKa = 3.73 TVDD571 pKa = 3.27 QTGLNPVDD579 pKa = 3.62 NAADD583 pKa = 3.65 SIYY586 pKa = 10.34 VRR588 pKa = 11.84 RR589 pKa = 11.84 PIEE592 pKa = 4.1 LYY594 pKa = 10.48 KK595 pKa = 10.87 SYY597 pKa = 11.28 DD598 pKa = 3.48 PASVVSGGEE607 pKa = 3.7 SRR609 pKa = 11.84 LTLRR613 pKa = 11.84 FQRR616 pKa = 11.84 NSRR619 pKa = 11.84 AGSTTSDD626 pKa = 2.36 ITFYY630 pKa = 11.41 DD631 pKa = 4.21 EE632 pKa = 5.22 LPSGHH637 pKa = 6.87 TIADD641 pKa = 3.52 PANIQNSCGGSVTISPDD658 pKa = 2.97 RR659 pKa = 11.84 SRR661 pKa = 11.84 VDD663 pKa = 3.42 LAGGSLGPVGGTTLEE678 pKa = 4.26 CTLVLTVIAPNSVGSSNNTVFAGHH702 pKa = 5.62 TTATDD707 pKa = 3.75 DD708 pKa = 4.14 GQPGAFNQMVTTRR721 pKa = 11.84 NRR723 pKa = 11.84 SATLTRR729 pKa = 11.84 TQGDD733 pKa = 3.61 LTLTKK738 pKa = 10.5 EE739 pKa = 4.25 FLQPTIAGGQPSRR752 pKa = 11.84 VRR754 pKa = 11.84 ITIANTASDD763 pKa = 5.15 AIDD766 pKa = 3.55 LTGVGITDD774 pKa = 3.96 SFAGTEE780 pKa = 3.97 LRR782 pKa = 11.84 LADD785 pKa = 3.89 SVSPTFTNTSGTPNAGGCTGGTFATVGGAGGQITLAGAGVEE826 pKa = 4.23 ADD828 pKa = 3.84 STCHH832 pKa = 6.72 FEE834 pKa = 4.96 FNTTSFFGGTVTNSLPAQALVSDD857 pKa = 3.99 QGTTNPAPVSASLAVEE873 pKa = 4.12 RR874 pKa = 11.84 EE875 pKa = 4.17 FNVSKK880 pKa = 10.64 GFQPAIVGPGEE891 pKa = 3.85 TSQAVISVINASSVNFTGTTGIPMLVDD918 pKa = 3.46 TLPAGLAISGTPTTTCTGATVSANTASSPQEE949 pKa = 4.0 IILSGGSYY957 pKa = 10.11 PADD960 pKa = 3.44 TSCTIFADD968 pKa = 4.05 VSAAAPGAFVNTIPAGSLSTEE989 pKa = 3.96 EE990 pKa = 4.62 SGTNADD996 pKa = 4.0 PASATLAVIAPPTIAKK1012 pKa = 10.26 AFGPASIGNGEE1023 pKa = 4.23 TSTITFTLSNPNAASLLPAGISGATFSDD1051 pKa = 3.42 TMTNMVIAAPPNVGGTCNPQTYY1073 pKa = 9.41 SASAGGSVFSVSDD1086 pKa = 3.43 LSIAPGASCTVQIDD1100 pKa = 3.72 VTSTVPGSHH1109 pKa = 7.51 DD1110 pKa = 3.56 NQATGISSDD1119 pKa = 3.28 QMANPGDD1126 pKa = 3.99 PSNIAALTVGTASVPTIAKK1145 pKa = 9.65 SFSVDD1150 pKa = 2.89 TLLPGEE1156 pKa = 4.27 TATLLFTLQNPNAGAANIDD1175 pKa = 3.37 AAGFSDD1181 pKa = 4.84 TFPTSPGAMVVASPANVYY1199 pKa = 7.44 STCDD1203 pKa = 3.16 GTVADD1208 pKa = 4.91 LGGGSVDD1215 pKa = 4.15 PGDD1218 pKa = 4.4 TGIQLQGGFIAGNGSCYY1235 pKa = 10.5 VSVDD1239 pKa = 3.19 VTVPTDD1245 pKa = 3.36 GTYY1248 pKa = 10.71 TNTSSGLTTEE1258 pKa = 5.17 FGTSGTASDD1267 pKa = 3.77 TLVAEE1272 pKa = 4.41 SAPTILSASEE1282 pKa = 3.82 GTRR1285 pKa = 11.84 TVRR1288 pKa = 11.84 EE1289 pKa = 4.06 SDD1291 pKa = 2.95 NSQVVDD1297 pKa = 4.1 SVLGPNDD1304 pKa = 3.36 TFAGQQAFVGSGAAGNVDD1322 pKa = 3.49 MTITAIDD1329 pKa = 3.42 AALSVNTDD1337 pKa = 2.66 TGLIVVAPGTGAGTYY1352 pKa = 10.07 SVTYY1356 pKa = 8.13 QICQAGNPSNCAPTRR1371 pKa = 11.84 TEE1373 pKa = 3.81 SVEE1376 pKa = 4.21 VVVATILATTDD1387 pKa = 3.64 YY1388 pKa = 11.43 ASGSPLQVEE1397 pKa = 4.28 QADD1400 pKa = 4.1 FAKK1403 pKa = 10.8 EE1404 pKa = 3.78 PGNVLGSSDD1413 pKa = 4.89 LLDD1416 pKa = 3.87 GVQATVAPGTGGNVDD1431 pKa = 4.23 LSILSVTGPSGASTDD1446 pKa = 2.81 ITVNVNTGVISVAGSTPQGIYY1467 pKa = 9.87 EE1468 pKa = 4.19 ISYY1471 pKa = 8.36 QICEE1475 pKa = 4.75 DD1476 pKa = 3.86 DD1477 pKa = 4.85 HH1478 pKa = 9.46 ADD1480 pKa = 3.69 NCDD1483 pKa = 3.34 TASEE1487 pKa = 4.3 FVEE1490 pKa = 4.37 VTIVPIAAGAEE1501 pKa = 4.04 TARR1504 pKa = 11.84 AATGSDD1510 pKa = 2.87 QSVNVGSLLGASDD1523 pKa = 4.08 TLDD1526 pKa = 3.71 GAPATVGPGATGSVDD1541 pKa = 3.55 LSVVSITEE1549 pKa = 4.17 GGSPSGNIAVDD1560 pKa = 4.2 LDD1562 pKa = 3.74 TGDD1565 pKa = 3.64 MVVAAGTPDD1574 pKa = 3.14 GTYY1577 pKa = 10.42 LVTYY1581 pKa = 8.07 RR1582 pKa = 11.84 ICEE1585 pKa = 3.76 EE1586 pKa = 4.27 TNRR1589 pKa = 11.84 SNCATATEE1597 pKa = 4.91 EE1598 pKa = 3.91 ITVTSLAIVAGPEE1611 pKa = 3.55 ADD1613 pKa = 3.5 RR1614 pKa = 11.84 TVVGSDD1620 pKa = 3.29 GAIDD1624 pKa = 4.19 AGPALSSAQDD1634 pKa = 3.33 RR1635 pKa = 11.84 VDD1637 pKa = 3.78 GSAATLGASGNATLSIVPGANTDD1660 pKa = 3.69 PEE1662 pKa = 4.49 LSIDD1666 pKa = 3.8 PVTSRR1671 pKa = 11.84 ITVAAGTAAGTYY1683 pKa = 7.98 TIEE1686 pKa = 4.45 YY1687 pKa = 9.23 RR1688 pKa = 11.84 LCDD1691 pKa = 3.32 STNSDD1696 pKa = 3.13 NCATAIEE1703 pKa = 4.38 TVIVADD1709 pKa = 4.73 RR1710 pKa = 11.84 PIAAAPEE1717 pKa = 4.06 PVQIVYY1723 pKa = 10.33 QSNSAVTAPSIIGASDD1739 pKa = 5.09 LIDD1742 pKa = 3.66 SAQAVPGFGGNVILTTVSGDD1762 pKa = 3.31 PRR1764 pKa = 11.84 LTLDD1768 pKa = 3.56 TEE1770 pKa = 4.23 TGEE1773 pKa = 4.54 IEE1775 pKa = 4.47 VAPNMPLGDD1784 pKa = 3.56 FTLTYY1789 pKa = 10.0 RR1790 pKa = 11.84 ICDD1793 pKa = 3.52 PANLDD1798 pKa = 3.44 NCATATEE1805 pKa = 4.48 TVRR1808 pKa = 11.84 IARR1811 pKa = 11.84 RR1812 pKa = 11.84 PITAASEE1819 pKa = 4.12 ADD1821 pKa = 3.62 RR1822 pKa = 11.84 PLYY1825 pKa = 10.78 ASTAAQTAGSVIGSGDD1841 pKa = 3.64 TLDD1844 pKa = 4.5 GLAAVPGFGGNVVLTLGGGSPEE1866 pKa = 3.84 VSLNVATGDD1875 pKa = 3.7 LGVAAGTPPGTYY1887 pKa = 10.43 VLDD1890 pKa = 4.1 YY1891 pKa = 11.2 NN1892 pKa = 4.57
Molecular weight: 189.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.897
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A0L1JKN8|A0A0L1JKN8_9RHOB Cytochrome B OS=Pseudaestuariivita atlantica OX=1317121 GN=ATO11_17005 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3978
0
3978
1234050
41
2946
310.2
33.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.817 ± 0.05
0.897 ± 0.014
6.561 ± 0.043
5.672 ± 0.038
3.706 ± 0.023
8.857 ± 0.047
2.082 ± 0.02
4.905 ± 0.025
2.817 ± 0.034
9.824 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.753 ± 0.021
2.412 ± 0.024
5.172 ± 0.034
2.969 ± 0.02
6.94 ± 0.039
4.747 ± 0.022
5.69 ± 0.033
7.522 ± 0.034
1.474 ± 0.017
2.182 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here