Altererythrobacter epoxidivorans
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2773 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M5KYF7|A0A0M5KYF7_9SPHN Protein GrpE OS=Altererythrobacter epoxidivorans OX=361183 GN=grpE PE=3 SV=1
MM1 pKa = 7.13 KK2 pKa = 9.56 TLSLALTAVLATGLVACTDD21 pKa = 3.64 EE22 pKa = 5.24 ASVEE26 pKa = 4.11 NTDD29 pKa = 4.87 LDD31 pKa = 3.92 VATTDD36 pKa = 3.5 GEE38 pKa = 4.29 AAAKK42 pKa = 10.18 ARR44 pKa = 11.84 EE45 pKa = 4.14 IQGGDD50 pKa = 3.48 LADD53 pKa = 4.04 LQLGAKK59 pKa = 9.26 IVGPQGEE66 pKa = 4.39 EE67 pKa = 3.66 VKK69 pKa = 10.94 ARR71 pKa = 11.84 LTNEE75 pKa = 3.47 AGAFADD81 pKa = 3.16 ITSYY85 pKa = 10.21 VACPKK90 pKa = 10.67 GVTEE94 pKa = 5.03 CDD96 pKa = 3.23 PATAPNGMIYY106 pKa = 9.73 TYY108 pKa = 11.15 VHH110 pKa = 5.47 TVYY113 pKa = 10.42 PGEE116 pKa = 4.72 DD117 pKa = 3.44 NQDD120 pKa = 3.49 DD121 pKa = 4.23 SGSGTGADD129 pKa = 3.51 SSDD132 pKa = 3.63 VEE134 pKa = 4.28 IATSFMMTQPAYY146 pKa = 10.35 GFTGAVGYY154 pKa = 8.05 STAEE158 pKa = 4.14 VIRR161 pKa = 11.84 AVGNKK166 pKa = 8.26 AQVVITCLDD175 pKa = 3.51 GGIVWTINAGGGGDD189 pKa = 3.01 QWEE192 pKa = 4.19 QAEE195 pKa = 5.29 PITFWWQSTVPPAGPADD212 pKa = 3.94 AYY214 pKa = 10.53 AIKK217 pKa = 10.57 ANYY220 pKa = 7.39 VTATGNGPYY229 pKa = 9.3 PAKK232 pKa = 10.39 SDD234 pKa = 3.71 QAPSACTAPSTDD246 pKa = 2.98 GG247 pKa = 3.49
Molecular weight: 25.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 0.807
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A0M4LTG0|A0A0M4LTG0_9SPHN Propionyl-CoA carboxylase beta chain OS=Altererythrobacter epoxidivorans OX=361183 GN=AMC99_00372 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.06 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.8 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.46 NLCAA44 pKa = 4.54
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2773
0
2773
853711
37
3761
307.9
33.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.111 ± 0.063
0.83 ± 0.017
6.28 ± 0.053
6.561 ± 0.04
3.693 ± 0.032
8.785 ± 0.044
1.936 ± 0.02
5.218 ± 0.031
3.53 ± 0.039
9.61 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.024
2.707 ± 0.028
4.952 ± 0.031
3.153 ± 0.023
6.725 ± 0.047
5.523 ± 0.034
5.051 ± 0.04
6.982 ± 0.032
1.426 ± 0.021
2.262 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here