Streptococcus phage Javan247
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A5Q5|A0A4D6A5Q5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan247 OX=2548069 GN=Javan247_0015 PE=4 SV=1
MM1 pKa = 8.0 DD2 pKa = 4.56 KK3 pKa = 11.2 VKK5 pKa = 10.77 LFQNEE10 pKa = 4.09 VLGSGFDD17 pKa = 3.23 IDD19 pKa = 6.24 GYY21 pKa = 10.9 FGWQCWDD28 pKa = 3.58 GYY30 pKa = 11.61 AKK32 pKa = 10.42 YY33 pKa = 10.01 CLWLGVPFSNCTDD46 pKa = 3.18 SLYY49 pKa = 11.45 VKK51 pKa = 10.14 DD52 pKa = 4.36 LWNQRR57 pKa = 11.84 HH58 pKa = 5.51 EE59 pKa = 4.37 NGILDD64 pKa = 3.85 YY65 pKa = 10.83 FDD67 pKa = 4.26 EE68 pKa = 4.56 VEE70 pKa = 4.04 NLEE73 pKa = 4.22 GGEE76 pKa = 3.9 VCIFVEE82 pKa = 4.97 SPLTPVSHH90 pKa = 5.93 VAIFVGDD97 pKa = 3.46 VDD99 pKa = 4.08 GVNGQFLGQNQGGAPGPFGGGAFDD123 pKa = 3.73 VQIFPYY129 pKa = 10.3 SILYY133 pKa = 6.8 PTAFRR138 pKa = 11.84 PKK140 pKa = 10.53 GEE142 pKa = 4.23 SLPKK146 pKa = 10.07 QEE148 pKa = 4.58 LKK150 pKa = 10.87 EE151 pKa = 4.54 AITEE155 pKa = 4.03 VMDD158 pKa = 3.4 NHH160 pKa = 6.12 EE161 pKa = 4.51 VPFFPEE167 pKa = 4.07 DD168 pKa = 3.14 ATFTVGDD175 pKa = 3.59 SPINVRR181 pKa = 11.84 RR182 pKa = 11.84 EE183 pKa = 3.64 PSLTGEE189 pKa = 4.08 IVAVYY194 pKa = 10.01 QPGDD198 pKa = 3.44 KK199 pKa = 10.36 VHH201 pKa = 6.55 YY202 pKa = 9.59 DD203 pKa = 3.47 SKK205 pKa = 11.33 GSNDD209 pKa = 3.13 GYY211 pKa = 11.06 RR212 pKa = 11.84 WISYY216 pKa = 9.87 VGEE219 pKa = 4.28 SGNRR223 pKa = 11.84 NYY225 pKa = 10.82 LAIGQTDD232 pKa = 3.5 EE233 pKa = 4.12 AGNRR237 pKa = 11.84 IDD239 pKa = 4.83 LWGEE243 pKa = 3.77 LSS245 pKa = 3.33
Molecular weight: 27.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.058
IPC2_protein 4.24
IPC_protein 4.202
Toseland 4.024
ProMoST 4.329
Dawson 4.177
Bjellqvist 4.317
Wikipedia 4.062
Rodwell 4.037
Grimsley 3.935
Solomon 4.164
Lehninger 4.126
Nozaki 4.279
DTASelect 4.469
Thurlkill 4.05
EMBOSS 4.075
Sillero 4.317
Patrickios 2.651
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.22
Protein with the highest isoelectric point:
>tr|A0A4D6A5V8|A0A4D6A5V8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan247 OX=2548069 GN=Javan247_0030 PE=4 SV=1
MM1 pKa = 7.73 TGRR4 pKa = 11.84 KK5 pKa = 9.63 DD6 pKa = 3.38 YY7 pKa = 10.83 FMPFGVPSGFSFALEE22 pKa = 4.14 HH23 pKa = 6.44 GVKK26 pKa = 10.51 LSNSAPRR33 pKa = 11.84 GVKK36 pKa = 9.5 QRR38 pKa = 11.84 SS39 pKa = 3.13
Molecular weight: 4.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.736
IPC_protein 10.218
Toseland 10.716
ProMoST 10.277
Dawson 10.804
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.213
Grimsley 10.833
Solomon 10.877
Lehninger 10.862
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 11.082
IPC_peptide 10.891
IPC2_peptide 9.033
IPC2.peptide.svr19 8.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
10741
38
1268
228.5
25.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.448 ± 1.034
0.419 ± 0.087
6.424 ± 0.498
6.908 ± 0.583
3.957 ± 0.193
6.824 ± 0.49
1.322 ± 0.205
6.601 ± 0.259
8.156 ± 0.517
7.951 ± 0.248
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.812 ± 0.296
5.623 ± 0.371
3.203 ± 0.263
4.394 ± 0.278
3.947 ± 0.301
6.666 ± 0.609
5.642 ± 0.304
7.029 ± 0.432
1.015 ± 0.14
3.659 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here