Psychroserpens burtonensis
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3458 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C7B7S8|A0A5C7B7S8_9FLAO Sigma-70 family RNA polymerase sigma factor OS=Psychroserpens burtonensis OX=49278 GN=ES692_14790 PE=3 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.49 NLIYY6 pKa = 10.31 IIAILLTVGMFQSCSDD22 pKa = 4.13 DD23 pKa = 4.23 DD24 pKa = 4.88 FPVPPASTVPEE35 pKa = 4.02 FAFVIDD41 pKa = 3.8 NDD43 pKa = 3.62 EE44 pKa = 4.24 FAPATVTFTNNSIVPGNVGTATYY67 pKa = 9.9 YY68 pKa = 10.31 WNFGDD73 pKa = 4.97 GEE75 pKa = 4.6 STSEE79 pKa = 3.98 EE80 pKa = 4.26 NPTYY84 pKa = 10.78 LYY86 pKa = 9.99 TEE88 pKa = 4.21 SGAYY92 pKa = 8.01 TVNLVVTTSEE102 pKa = 3.9 SLEE105 pKa = 3.86 INEE108 pKa = 3.85 ITKK111 pKa = 9.22 TVVIKK116 pKa = 10.64 DD117 pKa = 3.66 PNATGVPIYY126 pKa = 9.34 FTDD129 pKa = 4.62 GAQVYY134 pKa = 9.85 QGLINTQAPIFTTVNGVVAQSSYY157 pKa = 11.54 GMVIDD162 pKa = 4.09 NVNSKK167 pKa = 10.86 LYY169 pKa = 10.35 ISDD172 pKa = 3.62 YY173 pKa = 10.96 GADD176 pKa = 3.3 KK177 pKa = 10.41 IYY179 pKa = 10.65 RR180 pKa = 11.84 SNLDD184 pKa = 2.95 GSNFEE189 pKa = 4.16 EE190 pKa = 4.73 FRR192 pKa = 11.84 TGVDD196 pKa = 3.24 QPNGMTIDD204 pKa = 3.66 YY205 pKa = 10.0 EE206 pKa = 4.28 EE207 pKa = 4.14 NQIYY211 pKa = 9.86 WDD213 pKa = 3.86 TSSGIQRR220 pKa = 11.84 GNIDD224 pKa = 3.89 DD225 pKa = 3.98 TDD227 pKa = 3.83 VNQVEE232 pKa = 4.79 DD233 pKa = 4.32 FVTGQSNDD241 pKa = 3.52 PDD243 pKa = 4.12 GLSIDD248 pKa = 3.72 LVNRR252 pKa = 11.84 KK253 pKa = 9.55 LYY255 pKa = 7.87 WVNYY259 pKa = 9.37 NGGVWVKK266 pKa = 10.6 NLDD269 pKa = 3.65 GTGEE273 pKa = 4.15 SEE275 pKa = 5.04 LIPVIEE281 pKa = 4.75 GGSMLVIGDD290 pKa = 4.3 RR291 pKa = 11.84 IFYY294 pKa = 10.41 DD295 pKa = 3.78 EE296 pKa = 4.53 YY297 pKa = 11.0 VASGDD302 pKa = 3.72 VRR304 pKa = 11.84 LKK306 pKa = 10.96 SADD309 pKa = 3.71 LDD311 pKa = 3.72 GTNISTIAVNIGRR324 pKa = 11.84 VVYY327 pKa = 10.28 GIGYY331 pKa = 8.29 DD332 pKa = 3.4 ANEE335 pKa = 3.95 NKK337 pKa = 10.01 IYY339 pKa = 10.15 WGDD342 pKa = 3.52 RR343 pKa = 11.84 STDD346 pKa = 2.83 VMMRR350 pKa = 11.84 ANLDD354 pKa = 3.46 GSSPEE359 pKa = 3.43 AWFTHH364 pKa = 5.82 SADD367 pKa = 3.06 TRR369 pKa = 11.84 GIVIGEE375 pKa = 4.22 LEE377 pKa = 3.88
Molecular weight: 41.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.605
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.935
Patrickios 1.011
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A5C7BE50|A0A5C7BE50_9FLAO Uncharacterized protein OS=Psychroserpens burtonensis OX=49278 GN=ES692_09620 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.06 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.18 RR22 pKa = 11.84 MASVNGRR29 pKa = 11.84 KK30 pKa = 9.21 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.62 LSVSSEE48 pKa = 4.04 TRR50 pKa = 11.84 HH51 pKa = 5.93 KK52 pKa = 10.8 RR53 pKa = 3.27
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.789
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.544
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.296
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.018
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.08
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3458
0
3458
1151945
22
2740
333.1
37.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.119 ± 0.043
0.773 ± 0.016
5.985 ± 0.039
6.314 ± 0.041
5.27 ± 0.041
6.233 ± 0.045
1.752 ± 0.023
8.289 ± 0.039
7.53 ± 0.065
9.249 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.131 ± 0.021
6.394 ± 0.045
3.172 ± 0.02
3.375 ± 0.023
3.301 ± 0.029
6.76 ± 0.033
6.205 ± 0.053
6.138 ± 0.034
0.99 ± 0.015
4.018 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here