Arcobacter nitrofigilis (strain ATCC 33309 / DSM 7299 / LMG 7604 / NCTC 12251 / CI) (Campylobacter nitrofigilis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Arcobacter group; Arcobacter; Arcobacter nitrofigilis

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5V1H6|D5V1H6_ARCNC Type VI secretion system effector Hcp1 family OS=Arcobacter nitrofigilis (strain ATCC 33309 / DSM 7299 / LMG 7604 / NCTC 12251 / CI) OX=572480 GN=Arnit_1756 PE=4 SV=1
MM1 pKa = 7.41TLLLNKK7 pKa = 10.17LSLYY11 pKa = 9.75STFGVDD17 pKa = 5.38DD18 pKa = 4.5FEE20 pKa = 6.32SLDD23 pKa = 3.64KK24 pKa = 11.17VINNMAPSMVEE35 pKa = 3.88YY36 pKa = 10.58YY37 pKa = 10.92LSDD40 pKa = 3.67LSDD43 pKa = 3.5GFEE46 pKa = 3.54NVYY49 pKa = 10.63LNKK52 pKa = 10.35RR53 pKa = 11.84NIQNSLTIGDD63 pKa = 3.76FAIYY67 pKa = 10.24LDD69 pKa = 4.28YY70 pKa = 11.29SDD72 pKa = 5.71EE73 pKa = 4.47IYY75 pKa = 11.27LEE77 pKa = 4.48IDD79 pKa = 3.52DD80 pKa = 4.76NKK82 pKa = 11.0EE83 pKa = 3.87DD84 pKa = 5.22LEE86 pKa = 4.59TSSLWW91 pKa = 3.4

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5V191|D5V191_ARCNC Bifunctional purine biosynthesis protein PurH OS=Arcobacter nitrofigilis (strain ATCC 33309 / DSM 7299 / LMG 7604 / NCTC 12251 / CI) OX=572480 GN=purH PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.09QPHH8 pKa = 5.34NTPKK12 pKa = 10.47KK13 pKa = 7.58RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84VRR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84KK29 pKa = 8.95VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3125

0

3125

987378

30

6971

316.0

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.794 ± 0.052

0.898 ± 0.016

5.684 ± 0.05

6.936 ± 0.049

5.158 ± 0.045

5.58 ± 0.06

1.558 ± 0.021

9.598 ± 0.046

9.459 ± 0.068

9.712 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.026

6.388 ± 0.048

2.77 ± 0.027

2.682 ± 0.025

2.8 ± 0.031

6.746 ± 0.041

5.258 ± 0.053

5.861 ± 0.037

0.741 ± 0.015

4.05 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski