Granulicatella elegans ATCC 700633
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1562 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D0BLH7|D0BLH7_9LACT Uncharacterized protein OS=Granulicatella elegans ATCC 700633 OX=626369 GN=HMPREF0446_00812 PE=4 SV=1
MM1 pKa = 6.94 ATALIVYY8 pKa = 10.13 ASLTGNTEE16 pKa = 3.95 EE17 pKa = 4.84 IADD20 pKa = 3.75 ILAEE24 pKa = 4.04 SLEE27 pKa = 4.28 EE28 pKa = 3.88 QGVEE32 pKa = 4.05 VDD34 pKa = 3.87 IKK36 pKa = 10.61 EE37 pKa = 4.49 CTSATADD44 pKa = 3.49 EE45 pKa = 4.25 FHH47 pKa = 7.76 DD48 pKa = 3.84 YY49 pKa = 10.51 DD50 pKa = 3.54 ICVVATYY57 pKa = 9.26 TYY59 pKa = 9.74 GTDD62 pKa = 3.34 GDD64 pKa = 4.46 LPDD67 pKa = 5.25 EE68 pKa = 4.33 IVDD71 pKa = 4.41 FYY73 pKa = 11.84 EE74 pKa = 4.07 EE75 pKa = 4.79 LEE77 pKa = 4.2 EE78 pKa = 4.49 EE79 pKa = 4.33 DD80 pKa = 4.75 LSGKK84 pKa = 10.11 IYY86 pKa = 10.59 GALGSGDD93 pKa = 3.3 TFYY96 pKa = 11.52 DD97 pKa = 3.66 KK98 pKa = 10.96 FCQSVLDD105 pKa = 4.14 FDD107 pKa = 4.05 EE108 pKa = 5.18 QFAKK112 pKa = 10.21 TGATKK117 pKa = 10.33 GAEE120 pKa = 3.94 NVLVDD125 pKa = 4.98 LNAEE129 pKa = 4.08 EE130 pKa = 4.65 EE131 pKa = 4.22 DD132 pKa = 3.87 VKK134 pKa = 11.35 NIEE137 pKa = 4.36 AFAKK141 pKa = 10.62 SLVEE145 pKa = 4.06 KK146 pKa = 10.93 LGG148 pKa = 3.49
Molecular weight: 16.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.452
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.363
Solomon 3.617
Lehninger 3.567
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.77
Patrickios 0.985
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|D0BMB0|D0BMB0_9LACT Beta sliding clamp OS=Granulicatella elegans ATCC 700633 OX=626369 GN=HMPREF0446_00833 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.16 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 QKK14 pKa = 9.38 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.8 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLAAA44 pKa = 4.31
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1562
0
1562
504669
32
2889
323.1
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.795 ± 0.093
0.652 ± 0.02
5.032 ± 0.057
7.917 ± 0.067
4.476 ± 0.065
6.447 ± 0.077
1.956 ± 0.036
7.694 ± 0.092
7.196 ± 0.071
9.411 ± 0.134
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.524 ± 0.043
4.474 ± 0.042
3.479 ± 0.078
4.282 ± 0.056
3.659 ± 0.051
5.928 ± 0.054
6.046 ± 0.134
7.377 ± 0.076
0.884 ± 0.023
3.77 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here