Enterococcus sp. HSIEG1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; unclassified Enterococcus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3882 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0VKL5|T0VKL5_9ENTE Oligopeptide ABC transporter ATP-bindingprotein OS=Enterococcus sp. HSIEG1 OX=1316414 GN=HSIEG1_1345 PE=4 SV=1
MM1 pKa = 7.65DD2 pKa = 3.91QFAAEE7 pKa = 4.29NDD9 pKa = 3.37FTYY12 pKa = 10.62QIVDD16 pKa = 3.18IASFAARR23 pKa = 11.84SEE25 pKa = 4.25ALLSDD30 pKa = 3.73QVDD33 pKa = 3.64GAVYY37 pKa = 9.61TEE39 pKa = 4.62PQASMLAAQGAIVLGSSKK57 pKa = 10.47EE58 pKa = 3.91AGIKK62 pKa = 10.54GGTLQFMTDD71 pKa = 3.27VVKK74 pKa = 10.79DD75 pKa = 3.97RR76 pKa = 11.84PQDD79 pKa = 3.33VAAFYY84 pKa = 10.1QAYY87 pKa = 9.58DD88 pKa = 3.41QAIDD92 pKa = 3.45YY93 pKa = 7.29MNSHH97 pKa = 6.95KK98 pKa = 10.75ASEE101 pKa = 4.36YY102 pKa = 11.13ADD104 pKa = 3.36ILADD108 pKa = 3.78YY109 pKa = 8.85QFPDD113 pKa = 3.74AMSTYY118 pKa = 10.73LDD120 pKa = 3.97SQKK123 pKa = 10.97EE124 pKa = 4.22DD125 pKa = 3.3YY126 pKa = 10.29PYY128 pKa = 10.86AAKK131 pKa = 10.55VPQDD135 pKa = 3.49QLDD138 pKa = 4.43KK139 pKa = 10.86IVAWTKK145 pKa = 11.17DD146 pKa = 2.9KK147 pKa = 11.55GQIDD151 pKa = 3.36QAYY154 pKa = 8.13TYY156 pKa = 11.23DD157 pKa = 3.81EE158 pKa = 4.27LTDD161 pKa = 4.26FSFLANN167 pKa = 3.54

Molecular weight: 18.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0TXQ2|T0TXQ2_9ENTE Na+/H+ antiporter OS=Enterococcus sp. HSIEG1 OX=1316414 GN=HSIEG1_324 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.33GRR39 pKa = 11.84KK40 pKa = 9.06VISAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3882

0

3882

911084

37

2458

234.7

26.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.599 ± 0.047

0.679 ± 0.013

5.161 ± 0.036

7.12 ± 0.048

4.675 ± 0.039

6.516 ± 0.041

1.859 ± 0.021

7.521 ± 0.044

6.274 ± 0.038

10.044 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.023

4.3 ± 0.03

3.555 ± 0.024

4.643 ± 0.037

4.041 ± 0.031

6.061 ± 0.08

5.854 ± 0.038

6.71 ± 0.037

0.993 ± 0.015

3.653 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski