Amino acid dipepetide frequency for Enterococcus sp. HSIEG1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.072AlaAla: 7.072 ± 0.13
0.563AlaCys: 0.563 ± 0.023
4.024AlaAsp: 4.024 ± 0.068
5.304AlaGlu: 5.304 ± 0.089
3.542AlaPhe: 3.542 ± 0.069
5.226AlaGly: 5.226 ± 0.072
1.266AlaHis: 1.266 ± 0.037
6.237AlaIle: 6.237 ± 0.085
4.878AlaLys: 4.878 ± 0.078
7.58AlaLeu: 7.58 ± 0.103
2.137AlaMet: 2.137 ± 0.05
3.088AlaAsn: 3.088 ± 0.057
2.146AlaPro: 2.146 ± 0.052
3.055AlaGln: 3.055 ± 0.065
2.621AlaArg: 2.621 ± 0.055
4.499AlaSer: 4.499 ± 0.102
4.182AlaThr: 4.182 ± 0.075
5.412AlaVal: 5.412 ± 0.089
0.595AlaTrp: 0.595 ± 0.028
2.562AlaTyr: 2.562 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.393CysAla: 0.393 ± 0.021
0.108CysCys: 0.108 ± 0.011
0.316CysAsp: 0.316 ± 0.019
0.368CysGlu: 0.368 ± 0.021
0.404CysPhe: 0.404 ± 0.022
0.633CysGly: 0.633 ± 0.028
0.183CysHis: 0.183 ± 0.014
0.447CysIle: 0.447 ± 0.022
0.254CysLys: 0.254 ± 0.017
0.78CysLeu: 0.78 ± 0.031
0.227CysMet: 0.227 ± 0.016
0.203CysAsn: 0.203 ± 0.016
0.273CysPro: 0.273 ± 0.015
0.365CysGln: 0.365 ± 0.022
0.281CysArg: 0.281 ± 0.018
0.481CysSer: 0.481 ± 0.024
0.317CysThr: 0.317 ± 0.019
0.362CysVal: 0.362 ± 0.019
0.093CysTrp: 0.093 ± 0.01
0.303CysTyr: 0.303 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.445AspAla: 3.445 ± 0.069
0.369AspCys: 0.369 ± 0.021
2.331AspAsp: 2.331 ± 0.062
3.935AspGlu: 3.935 ± 0.076
2.839AspPhe: 2.839 ± 0.061
3.426AspGly: 3.426 ± 0.066
1.179AspHis: 1.179 ± 0.038
3.485AspIle: 3.485 ± 0.067
2.835AspLys: 2.835 ± 0.054
5.153AspLeu: 5.153 ± 0.073
1.228AspMet: 1.228 ± 0.041
1.834AspAsn: 1.834 ± 0.046
2.262AspPro: 2.262 ± 0.048
2.818AspGln: 2.818 ± 0.059
2.315AspArg: 2.315 ± 0.048
2.876AspSer: 2.876 ± 0.097
2.726AspThr: 2.726 ± 0.06
3.292AspVal: 3.292 ± 0.064
0.695AspTrp: 0.695 ± 0.028
2.565AspTyr: 2.565 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
5.659GluAla: 5.659 ± 0.09
0.328GluCys: 0.328 ± 0.021
3.437GluAsp: 3.437 ± 0.059
6.239GluGlu: 6.239 ± 0.086
2.378GluPhe: 2.378 ± 0.053
3.869GluGly: 3.869 ± 0.073
1.102GluHis: 1.102 ± 0.036
5.385GluIle: 5.385 ± 0.093
6.005GluLys: 6.005 ± 0.083
6.781GluLeu: 6.781 ± 0.089
2.297GluMet: 2.297 ± 0.048
3.746GluAsn: 3.746 ± 0.066
1.994GluPro: 1.994 ± 0.044
3.57GluGln: 3.57 ± 0.072
3.037GluArg: 3.037 ± 0.064
3.743GluSer: 3.743 ± 0.129
4.294GluThr: 4.294 ± 0.077
4.607GluVal: 4.607 ± 0.07
0.698GluTrp: 0.698 ± 0.029
2.029GluTyr: 2.029 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.406PheAla: 3.406 ± 0.065
0.401PheCys: 0.401 ± 0.021
2.809PheAsp: 2.809 ± 0.062
2.771PheGlu: 2.771 ± 0.056
2.623PhePhe: 2.623 ± 0.064
3.288PheGly: 3.288 ± 0.067
0.888PheHis: 0.888 ± 0.031
3.243PheIle: 3.243 ± 0.066
2.056PheLys: 2.056 ± 0.053
5.166PheLeu: 5.166 ± 0.095
1.26PheMet: 1.26 ± 0.039
1.788PheAsn: 1.788 ± 0.042
1.692PhePro: 1.692 ± 0.045
1.927PheGln: 1.927 ± 0.049
1.605PheArg: 1.605 ± 0.043
3.502PheSer: 3.502 ± 0.069
2.571PheThr: 2.571 ± 0.058
3.261PheVal: 3.261 ± 0.063
0.538PheTrp: 0.538 ± 0.025
1.953PheTyr: 1.953 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
4.534GlyAla: 4.534 ± 0.082
0.564GlyCys: 0.564 ± 0.026
3.039GlyAsp: 3.039 ± 0.066
3.993GlyGlu: 3.993 ± 0.066
3.192GlyPhe: 3.192 ± 0.063
4.418GlyGly: 4.418 ± 0.086
1.255GlyHis: 1.255 ± 0.042
5.969GlyIle: 5.969 ± 0.088
4.519GlyLys: 4.519 ± 0.079
6.473GlyLeu: 6.473 ± 0.09
1.994GlyMet: 1.994 ± 0.054
2.765GlyAsn: 2.765 ± 0.052
1.573GlyPro: 1.573 ± 0.043
2.515GlyGln: 2.515 ± 0.06
2.452GlyArg: 2.452 ± 0.054
3.783GlySer: 3.783 ± 0.084
4.101GlyThr: 4.101 ± 0.07
4.554GlyVal: 4.554 ± 0.074
0.712GlyTrp: 0.712 ± 0.029
2.754GlyTyr: 2.754 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.217HisAla: 1.217 ± 0.038
0.169HisCys: 0.169 ± 0.014
0.82HisAsp: 0.82 ± 0.031
1.232HisGlu: 1.232 ± 0.039
1.077HisPhe: 1.077 ± 0.034
1.239HisGly: 1.239 ± 0.043
0.525HisHis: 0.525 ± 0.024
1.105HisIle: 1.105 ± 0.035
0.774HisLys: 0.774 ± 0.032
2.108HisLeu: 2.108 ± 0.048
0.474HisMet: 0.474 ± 0.023
0.615HisAsn: 0.615 ± 0.023
0.952HisPro: 0.952 ± 0.034
1.033HisGln: 1.033 ± 0.032
0.825HisArg: 0.825 ± 0.031
1.159HisSer: 1.159 ± 0.036
0.955HisThr: 0.955 ± 0.033
1.129HisVal: 1.129 ± 0.036
0.252HisTrp: 0.252 ± 0.017
0.927HisTyr: 0.927 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.351IleAla: 6.351 ± 0.082
0.625IleCys: 0.625 ± 0.024
4.692IleAsp: 4.692 ± 0.072
5.101IleGlu: 5.101 ± 0.073
3.292IlePhe: 3.292 ± 0.07
5.454IleGly: 5.454 ± 0.089
1.552IleHis: 1.552 ± 0.041
4.992IleIle: 4.992 ± 0.077
4.214IleLys: 4.214 ± 0.074
7.026IleLeu: 7.026 ± 0.111
1.707IleMet: 1.707 ± 0.045
3.228IleAsn: 3.228 ± 0.061
3.176IlePro: 3.176 ± 0.056
3.197IleGln: 3.197 ± 0.064
3.207IleArg: 3.207 ± 0.061
4.73IleSer: 4.73 ± 0.095
4.269IleThr: 4.269 ± 0.067
5.23IleVal: 5.23 ± 0.077
0.618IleTrp: 0.618 ± 0.025
2.549IleTyr: 2.549 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
4.476LysAla: 4.476 ± 0.07
0.238LysCys: 0.238 ± 0.016
3.373LysAsp: 3.373 ± 0.066
6.446LysGlu: 6.446 ± 0.093
1.786LysPhe: 1.786 ± 0.04
3.531LysGly: 3.531 ± 0.067
0.985LysHis: 0.985 ± 0.035
4.714LysIle: 4.714 ± 0.075
5.499LysLys: 5.499 ± 0.098
4.801LysLeu: 4.801 ± 0.066
2.139LysMet: 2.139 ± 0.047
3.473LysAsn: 3.473 ± 0.063
1.774LysPro: 1.774 ± 0.05
3.261LysGln: 3.261 ± 0.061
3.057LysArg: 3.057 ± 0.063
3.029LysSer: 3.029 ± 0.056
3.617LysThr: 3.617 ± 0.061
4.033LysVal: 4.033 ± 0.077
0.564LysTrp: 0.564 ± 0.025
1.946LysTyr: 1.946 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
8.583LeuAla: 8.583 ± 0.1
0.687LeuCys: 0.687 ± 0.025
4.895LeuAsp: 4.895 ± 0.067
5.952LeuGlu: 5.952 ± 0.085
5.266LeuPhe: 5.266 ± 0.092
6.201LeuGly: 6.201 ± 0.1
1.633LeuHis: 1.633 ± 0.045
7.302LeuIle: 7.302 ± 0.113
5.826LeuLys: 5.826 ± 0.083
11.104LeuLeu: 11.104 ± 0.164
2.628LeuMet: 2.628 ± 0.055
4.074LeuAsn: 4.074 ± 0.067
4.328LeuPro: 4.328 ± 0.07
4.12LeuGln: 4.12 ± 0.069
3.75LeuArg: 3.75 ± 0.07
7.12LeuSer: 7.12 ± 0.125
6.399LeuThr: 6.399 ± 0.087
6.626LeuVal: 6.626 ± 0.097
0.854LeuTrp: 0.854 ± 0.03
3.085LeuTyr: 3.085 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.208MetAla: 2.208 ± 0.051
0.14MetCys: 0.14 ± 0.011
1.451MetAsp: 1.451 ± 0.039
1.674MetGlu: 1.674 ± 0.045
0.936MetPhe: 0.936 ± 0.035
1.692MetGly: 1.692 ± 0.046
0.412MetHis: 0.412 ± 0.018
2.505MetIle: 2.505 ± 0.057
2.248MetLys: 2.248 ± 0.049
2.391MetLeu: 2.391 ± 0.054
0.9MetMet: 0.9 ± 0.034
1.552MetAsn: 1.552 ± 0.04
1.014MetPro: 1.014 ± 0.031
1.076MetGln: 1.076 ± 0.035
1.07MetArg: 1.07 ± 0.034
1.613MetSer: 1.613 ± 0.048
1.876MetThr: 1.876 ± 0.045
1.797MetVal: 1.797 ± 0.051
0.181MetTrp: 0.181 ± 0.015
0.691MetTyr: 0.691 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.73AsnAla: 2.73 ± 0.059
0.352AsnCys: 0.352 ± 0.019
2.315AsnAsp: 2.315 ± 0.051
3.319AsnGlu: 3.319 ± 0.056
1.814AsnPhe: 1.814 ± 0.042
3.26AsnGly: 3.26 ± 0.071
1.041AsnHis: 1.041 ± 0.037
2.809AsnIle: 2.809 ± 0.054
2.458AsnLys: 2.458 ± 0.054
3.865AsnLeu: 3.865 ± 0.064
1.118AsnMet: 1.118 ± 0.033
1.844AsnAsn: 1.844 ± 0.052
1.851AsnPro: 1.851 ± 0.041
2.688AsnGln: 2.688 ± 0.052
1.934AsnArg: 1.934 ± 0.057
2.23AsnSer: 2.23 ± 0.066
2.174AsnThr: 2.174 ± 0.053
2.782AsnVal: 2.782 ± 0.063
0.499AsnTrp: 0.499 ± 0.028
1.917AsnTyr: 1.917 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
2.638ProAla: 2.638 ± 0.059
0.153ProCys: 0.153 ± 0.013
1.921ProAsp: 1.921 ± 0.042
3.167ProGlu: 3.167 ± 0.056
1.972ProPhe: 1.972 ± 0.046
1.882ProGly: 1.882 ± 0.042
0.698ProHis: 0.698 ± 0.029
2.775ProIle: 2.775 ± 0.06
2.162ProLys: 2.162 ± 0.049
3.644ProLeu: 3.644 ± 0.071
0.909ProMet: 0.909 ± 0.032
1.585ProAsn: 1.585 ± 0.038
0.712ProPro: 0.712 ± 0.026
1.6ProGln: 1.6 ± 0.05
1.073ProArg: 1.073 ± 0.031
2.068ProSer: 2.068 ± 0.054
2.26ProThr: 2.26 ± 0.053
2.6ProVal: 2.6 ± 0.055
0.351ProTrp: 0.351 ± 0.019
1.379ProTyr: 1.379 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
3.765GlnAla: 3.765 ± 0.06
0.194GlnCys: 0.194 ± 0.014
1.9GlnAsp: 1.9 ± 0.04
3.65GlnGlu: 3.65 ± 0.066
1.743GlnPhe: 1.743 ± 0.043
2.524GlnGly: 2.524 ± 0.054
0.753GlnHis: 0.753 ± 0.028
3.182GlnIle: 3.182 ± 0.067
3.364GlnLys: 3.364 ± 0.054
5.185GlnLeu: 5.185 ± 0.09
1.348GlnMet: 1.348 ± 0.04
1.832GlnAsn: 1.832 ± 0.051
1.579GlnPro: 1.579 ± 0.05
2.943GlnGln: 2.943 ± 0.07
2.246GlnArg: 2.246 ± 0.058
2.548GlnSer: 2.548 ± 0.057
2.816GlnThr: 2.816 ± 0.051
3.039GlnVal: 3.039 ± 0.061
0.531GlnTrp: 0.531 ± 0.025
1.291GlnTyr: 1.291 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
2.274ArgAla: 2.274 ± 0.053
0.259ArgCys: 0.259 ± 0.019
1.758ArgAsp: 1.758 ± 0.043
2.832ArgGlu: 2.832 ± 0.064
2.173ArgPhe: 2.173 ± 0.051
2.257ArgGly: 2.257 ± 0.054
0.751ArgHis: 0.751 ± 0.03
3.192ArgIle: 3.192 ± 0.066
2.964ArgLys: 2.964 ± 0.059
4.304ArgLeu: 4.304 ± 0.07
1.248ArgMet: 1.248 ± 0.042
1.821ArgAsn: 1.821 ± 0.054
1.374ArgPro: 1.374 ± 0.038
2.073ArgGln: 2.073 ± 0.046
1.96ArgArg: 1.96 ± 0.052
2.42ArgSer: 2.42 ± 0.055
2.144ArgThr: 2.144 ± 0.042
2.417ArgVal: 2.417 ± 0.054
0.448ArgTrp: 0.448 ± 0.021
1.746ArgTyr: 1.746 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
4.241SerAla: 4.241 ± 0.098
0.408SerCys: 0.408 ± 0.023
3.097SerAsp: 3.097 ± 0.098
4.356SerGlu: 4.356 ± 0.143
3.339SerPhe: 3.339 ± 0.062
4.552SerGly: 4.552 ± 0.071
1.126SerHis: 1.126 ± 0.037
4.569SerIle: 4.569 ± 0.103
3.386SerLys: 3.386 ± 0.059
6.352SerLeu: 6.352 ± 0.125
1.606SerMet: 1.606 ± 0.048
2.541SerAsn: 2.541 ± 0.071
1.897SerPro: 1.897 ± 0.048
2.587SerGln: 2.587 ± 0.059
2.29SerArg: 2.29 ± 0.061
3.924SerSer: 3.924 ± 0.121
3.3SerThr: 3.3 ± 0.166
4.018SerVal: 4.018 ± 0.079
0.667SerTrp: 0.667 ± 0.027
2.354SerTyr: 2.354 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
4.531ThrAla: 4.531 ± 0.086
0.336ThrCys: 0.336 ± 0.02
3.145ThrAsp: 3.145 ± 0.06
3.831ThrGlu: 3.831 ± 0.064
2.792ThrPhe: 2.792 ± 0.059
4.101ThrGly: 4.101 ± 0.066
1.093ThrHis: 1.093 ± 0.035
5.204ThrIle: 5.204 ± 0.072
3.43ThrLys: 3.43 ± 0.061
5.492ThrLeu: 5.492 ± 0.074
1.373ThrMet: 1.373 ± 0.041
2.6ThrAsn: 2.6 ± 0.059
2.402ThrPro: 2.402 ± 0.056
1.986ThrGln: 1.986 ± 0.044
1.927ThrArg: 1.927 ± 0.045
3.542ThrSer: 3.542 ± 0.175
3.754ThrThr: 3.754 ± 0.078
4.346ThrVal: 4.346 ± 0.077
0.516ThrTrp: 0.516 ± 0.025
2.136ThrTyr: 2.136 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
5.636ValAla: 5.636 ± 0.098
0.535ValCys: 0.535 ± 0.025
3.679ValAsp: 3.679 ± 0.069
4.176ValGlu: 4.176 ± 0.071
3.102ValPhe: 3.102 ± 0.059
4.447ValGly: 4.447 ± 0.075
1.028ValHis: 1.028 ± 0.033
5.36ValIle: 5.36 ± 0.078
3.802ValLys: 3.802 ± 0.075
6.807ValLeu: 6.807 ± 0.09
1.788ValMet: 1.788 ± 0.045
2.656ValAsn: 2.656 ± 0.06
2.732ValPro: 2.732 ± 0.053
2.276ValGln: 2.276 ± 0.048
2.495ValArg: 2.495 ± 0.054
4.541ValSer: 4.541 ± 0.097
4.35ValThr: 4.35 ± 0.075
4.813ValVal: 4.813 ± 0.091
0.558ValTrp: 0.558 ± 0.023
2.318ValTyr: 2.318 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.547TrpAla: 0.547 ± 0.022
0.069TrpCys: 0.069 ± 0.01
0.493TrpAsp: 0.493 ± 0.022
0.577TrpGlu: 0.577 ± 0.025
0.487TrpPhe: 0.487 ± 0.027
0.649TrpGly: 0.649 ± 0.028
0.224TrpHis: 0.224 ± 0.016
0.667TrpIle: 0.667 ± 0.025
0.54TrpLys: 0.54 ± 0.025
1.235TrpLeu: 1.235 ± 0.041
0.286TrpMet: 0.286 ± 0.016
0.436TrpAsn: 0.436 ± 0.023
0.258TrpPro: 0.258 ± 0.018
0.817TrpGln: 0.817 ± 0.033
0.435TrpArg: 0.435 ± 0.02
0.607TrpSer: 0.607 ± 0.023
0.539TrpThr: 0.539 ± 0.025
0.578TrpVal: 0.578 ± 0.028
0.124TrpTrp: 0.124 ± 0.012
0.364TrpTyr: 0.364 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.28TyrAla: 2.28 ± 0.06
0.294TyrCys: 0.294 ± 0.019
2.113TyrAsp: 2.113 ± 0.055
2.278TyrGlu: 2.278 ± 0.051
1.99TyrPhe: 1.99 ± 0.046
2.508TyrGly: 2.508 ± 0.059
0.896TyrHis: 0.896 ± 0.035
2.067TyrIle: 2.067 ± 0.053
1.529TyrLys: 1.529 ± 0.039
4.19TyrLeu: 4.19 ± 0.084
0.758TyrMet: 0.758 ± 0.034
1.322TyrAsn: 1.322 ± 0.039
1.56TyrPro: 1.56 ± 0.041
2.526TyrGln: 2.526 ± 0.058
1.834TyrArg: 1.834 ± 0.048
2.196TyrSer: 2.196 ± 0.057
1.893TyrThr: 1.893 ± 0.053
2.202TyrVal: 2.202 ± 0.05
0.437TyrTrp: 0.437 ± 0.022
1.664TyrTyr: 1.664 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3882 proteins (911084 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski