Amantichitinum ursilacus
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4368 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N0XJM5|A0A0N0XJM5_9NEIS Ion-translocating oxidoreductase complex subunit B OS=Amantichitinum ursilacus OX=857265 GN=rnfB PE=3 SV=1
MM1 pKa = 7.12 NAVAEE6 pKa = 4.4 MPSPFVFTDD15 pKa = 3.64 SAAGKK20 pKa = 9.37 VRR22 pKa = 11.84 EE23 pKa = 4.49 LIEE26 pKa = 4.51 EE27 pKa = 4.25 EE28 pKa = 4.39 GNPDD32 pKa = 2.85 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.94 GFTFDD54 pKa = 4.81 EE55 pKa = 4.64 ITNEE59 pKa = 4.36 DD60 pKa = 3.45 DD61 pKa = 3.59 TAVLKK66 pKa = 11.14 SGVTLLVDD74 pKa = 3.69 PMSYY78 pKa = 10.34 QYY80 pKa = 11.57 LVGAEE85 pKa = 3.69 IDD87 pKa = 3.85 YY88 pKa = 11.28 VEE90 pKa = 4.54 SLEE93 pKa = 4.67 GSQFTIKK100 pKa = 10.63 NPNATSTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.732
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.151
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A0N0XK06|A0A0N0XK06_9NEIS Epimerase domain-containing protein OS=Amantichitinum ursilacus OX=857265 GN=WG78_06245 PE=4 SV=1
MM1 pKa = 7.08 TFKK4 pKa = 10.98 LATRR8 pKa = 11.84 PVPLATAAALFWAGNFVAGRR28 pKa = 11.84 AINGEE33 pKa = 4.24 LSPLSLSFGRR43 pKa = 11.84 WILALLCLAPLALPRR58 pKa = 11.84 LRR60 pKa = 11.84 GAWPQLKK67 pKa = 8.53 QHH69 pKa = 5.55 WRR71 pKa = 11.84 TVLVLGLLGVTLCNTLIYY89 pKa = 10.3 RR90 pKa = 11.84 GVAHH94 pKa = 5.92 TTAVNAVMLNAFVPIMVLLAGRR116 pKa = 11.84 MFLGQSMSWMQCIGSALSFCGVAVILTHH144 pKa = 6.59 GAPSQLLSLNLNPGDD159 pKa = 3.58 LWVFAAVACWALYY172 pKa = 9.7 TLTLRR177 pKa = 11.84 KK178 pKa = 10.02 VPTGIDD184 pKa = 3.51 KK185 pKa = 10.69 IAMLFATILVGTLMLAPAVIWEE207 pKa = 4.28 WAHH210 pKa = 5.77 QGVPTLSVHH219 pKa = 5.85 SASIVLFLGIFPSVLAYY236 pKa = 10.55 LFYY239 pKa = 10.96 NIAVAQLGAARR250 pKa = 11.84 ASSFMHH256 pKa = 7.09 LIPAFGAMLAMLLLGEE272 pKa = 4.63 HH273 pKa = 5.39 VQWWHH278 pKa = 6.02 LAGLANVLAGVALSNGMLPRR298 pKa = 11.84 LNMPRR303 pKa = 11.84 TALRR307 pKa = 3.64
Molecular weight: 33.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.706
IPC_protein 10.599
Toseland 10.496
ProMoST 10.57
Dawson 10.657
Bjellqvist 10.438
Wikipedia 10.891
Rodwell 10.774
Grimsley 10.716
Solomon 10.76
Lehninger 10.716
Nozaki 10.555
DTASelect 10.409
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.599
Patrickios 10.496
IPC_peptide 10.76
IPC2_peptide 9.823
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4368
0
4368
1441360
30
4351
330.0
35.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.476 ± 0.063
0.848 ± 0.01
5.422 ± 0.025
4.807 ± 0.043
3.524 ± 0.026
7.886 ± 0.044
2.328 ± 0.019
4.798 ± 0.027
3.291 ± 0.034
10.867 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.019
3.203 ± 0.036
5.087 ± 0.035
4.577 ± 0.028
6.062 ± 0.038
5.47 ± 0.035
5.548 ± 0.039
7.358 ± 0.029
1.542 ± 0.02
2.56 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here