Nocardioides sp. Root1257

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7BHV4|A0A0Q7BHV4_9ACTN Chaperone protein DnaJ OS=Nocardioides sp. Root1257 OX=1736439 GN=dnaJ PE=3 SV=1
MM1 pKa = 7.18TMSDD5 pKa = 3.24EE6 pKa = 4.52SNNFDD11 pKa = 3.08EE12 pKa = 4.54TRR14 pKa = 11.84PIEE17 pKa = 4.76RR18 pKa = 11.84SPQPPAYY25 pKa = 9.87DD26 pKa = 3.83PYY28 pKa = 10.88GNPYY32 pKa = 9.01QDD34 pKa = 3.93PYY36 pKa = 11.46GGPFQQAAPHH46 pKa = 6.21PAPVRR51 pKa = 11.84AARR54 pKa = 11.84SGWSWRR60 pKa = 11.84SSLAGAVAGGVIAASVAVPVSWALASGGGQSTVADD95 pKa = 4.44APATAPQQLPEE106 pKa = 5.25PGDD109 pKa = 3.81SGSSGQAPDD118 pKa = 3.39TGQQGGTDD126 pKa = 3.6PFGYY130 pKa = 9.87GAPGGQLGGSTSNQTGDD147 pKa = 3.44QTDD150 pKa = 3.29ASADD154 pKa = 3.48QSQGVVLIDD163 pKa = 3.55TEE165 pKa = 4.65TTAGEE170 pKa = 3.92AAGTGLVLDD179 pKa = 3.99SSGIVLTNYY188 pKa = 9.94HH189 pKa = 5.37VVEE192 pKa = 4.87GSTSVKK198 pKa = 9.0VTIATTGATYY208 pKa = 9.98DD209 pKa = 3.8AKK211 pKa = 11.29VLGHH215 pKa = 6.24DD216 pKa = 3.69QEE218 pKa = 6.52ADD220 pKa = 3.49VALLQLDD227 pKa = 4.42GASGLATVKK236 pKa = 10.62LDD238 pKa = 4.46DD239 pKa = 5.62DD240 pKa = 4.81GDD242 pKa = 4.03PAVSDD247 pKa = 3.55EE248 pKa = 4.17VTAVGNAQGQGYY260 pKa = 9.8LSASTGSVVDD270 pKa = 4.76LDD272 pKa = 4.18QSIDD276 pKa = 3.67TQSEE280 pKa = 4.53GTVEE284 pKa = 4.45GEE286 pKa = 4.09HH287 pKa = 5.69LTGLIEE293 pKa = 4.0TNAYY297 pKa = 9.36VVGGYY302 pKa = 9.85SGGALLDD309 pKa = 3.82SEE311 pKa = 5.11GEE313 pKa = 4.39VVGITTAASSGGVAQSYY330 pKa = 9.54AVPIEE335 pKa = 4.56DD336 pKa = 4.18ALQVVQKK343 pKa = 10.52IEE345 pKa = 4.76DD346 pKa = 3.63GSEE349 pKa = 3.85TDD351 pKa = 3.73GVQVGASPYY360 pKa = 10.18LGIAISQDD368 pKa = 3.0ATGTSGGVEE377 pKa = 4.03VGQVQSGGAAAQAGIEE393 pKa = 4.09AGATITGIDD402 pKa = 3.42GTAITSYY409 pKa = 11.38DD410 pKa = 3.63VLKK413 pKa = 10.16STLATYY419 pKa = 10.49QPGDD423 pKa = 3.64SVTLRR428 pKa = 11.84WADD431 pKa = 3.4TSGATHH437 pKa = 6.79SAQVTLGEE445 pKa = 4.44SPVNN449 pKa = 3.57

Molecular weight: 44.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7BPE0|A0A0Q7BPE0_9ACTN Uncharacterized protein OS=Nocardioides sp. Root1257 OX=1736439 GN=ASC77_12140 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.24GRR40 pKa = 11.84KK41 pKa = 8.84SLAVV45 pKa = 3.3

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5063

0

5063

1661918

35

2517

328.2

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.969 ± 0.05

0.704 ± 0.008

6.68 ± 0.028

5.517 ± 0.035

2.811 ± 0.019

9.212 ± 0.033

2.158 ± 0.018

3.566 ± 0.028

1.97 ± 0.029

10.155 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.842 ± 0.015

1.785 ± 0.022

5.549 ± 0.03

2.79 ± 0.018

7.313 ± 0.048

5.409 ± 0.028

6.391 ± 0.043

9.552 ± 0.033

1.566 ± 0.014

2.061 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski