Amino acid dipepetide frequency for Nocardioides sp. Root1257

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.237AlaAla: 19.237 ± 0.169	0.933AlaCys: 0.933 ± 0.024	8.743AlaAsp: 8.743 ± 0.086	7.607AlaGlu: 7.607 ± 0.086	3.501AlaPhe: 3.501 ± 0.05	12.367AlaGly: 12.367 ± 0.091	2.471AlaHis: 2.471 ± 0.038	4.658AlaIle: 4.658 ± 0.057	2.549AlaLys: 2.549 ± 0.054	13.067AlaLeu: 13.067 ± 0.122	2.719AlaMet: 2.719 ± 0.046	2.077AlaAsn: 2.077 ± 0.038	6.128AlaPro: 6.128 ± 0.071	3.444AlaGln: 3.444 ± 0.047	8.91AlaArg: 8.91 ± 0.091	6.644AlaSer: 6.644 ± 0.073	8.131AlaThr: 8.131 ± 0.106	11.792AlaVal: 11.792 ± 0.102	2.022AlaTrp: 2.022 ± 0.039	2.694AlaTyr: 2.694 ± 0.039	0.001AlaXaa: 0.001 ± 0.001
Cys	0.853CysAla: 0.853 ± 0.025	0.082CysCys: 0.082 ± 0.006	0.475CysAsp: 0.475 ± 0.018	0.337CysGlu: 0.337 ± 0.014	0.23CysPhe: 0.23 ± 0.013	0.796CysGly: 0.796 ± 0.023	0.179CysHis: 0.179 ± 0.009	0.192CysIle: 0.192 ± 0.01	0.1CysLys: 0.1 ± 0.009	0.687CysLeu: 0.687 ± 0.023	0.108CysMet: 0.108 ± 0.008	0.137CysAsn: 0.137 ± 0.009	0.402CysPro: 0.402 ± 0.017	0.17CysGln: 0.17 ± 0.009	0.495CysArg: 0.495 ± 0.019	0.466CysSer: 0.466 ± 0.017	0.482CysThr: 0.482 ± 0.018	0.583CysVal: 0.583 ± 0.019	0.119CysTrp: 0.119 ± 0.01	0.149CysTyr: 0.149 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.28AspAla: 8.28 ± 0.089	0.356AspCys: 0.356 ± 0.014	4.917AspAsp: 4.917 ± 0.066	4.487AspGlu: 4.487 ± 0.058	1.731AspPhe: 1.731 ± 0.031	6.745AspGly: 6.745 ± 0.079	1.579AspHis: 1.579 ± 0.036	1.938AspIle: 1.938 ± 0.036	1.123AspLys: 1.123 ± 0.03	7.58AspLeu: 7.58 ± 0.074	0.854AspMet: 0.854 ± 0.023	1.054AspAsn: 1.054 ± 0.024	4.729AspPro: 4.729 ± 0.058	2.043AspGln: 2.043 ± 0.037	4.973AspArg: 4.973 ± 0.057	2.59AspSer: 2.59 ± 0.042	3.124AspThr: 3.124 ± 0.046	6.35AspVal: 6.35 ± 0.066	1.055AspTrp: 1.055 ± 0.03	1.289AspTyr: 1.289 ± 0.026	0.001AspXaa: 0.001 ± 0.001
Glu	6.622GluAla: 6.622 ± 0.077	0.297GluCys: 0.297 ± 0.013	3.027GluAsp: 3.027 ± 0.054	3.052GluGlu: 3.052 ± 0.06	1.459GluPhe: 1.459 ± 0.034	3.919GluGly: 3.919 ± 0.048	1.599GluHis: 1.599 ± 0.031	2.478GluIle: 2.478 ± 0.044	1.232GluLys: 1.232 ± 0.034	6.496GluLeu: 6.496 ± 0.078	1.068GluMet: 1.068 ± 0.03	0.85GluAsn: 0.85 ± 0.025	3.099GluPro: 3.099 ± 0.059	2.205GluGln: 2.205 ± 0.045	4.907GluArg: 4.907 ± 0.072	2.729GluSer: 2.729 ± 0.045	3.015GluThr: 3.015 ± 0.042	5.291GluVal: 5.291 ± 0.06	0.818GluTrp: 0.818 ± 0.025	1.002GluTyr: 1.002 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.605PheAla: 3.605 ± 0.054	0.268PheCys: 0.268 ± 0.013	2.154PheAsp: 2.154 ± 0.036	1.605PheGlu: 1.605 ± 0.034	0.931PhePhe: 0.931 ± 0.025	3.022PheGly: 3.022 ± 0.043	0.601PheHis: 0.601 ± 0.018	0.824PheIle: 0.824 ± 0.025	0.517PheLys: 0.517 ± 0.02	2.641PheLeu: 2.641 ± 0.048	0.429PheMet: 0.429 ± 0.018	0.6PheAsn: 0.6 ± 0.02	1.312PhePro: 1.312 ± 0.03	0.715PheGln: 0.715 ± 0.021	1.706PheArg: 1.706 ± 0.032	1.519PheSer: 1.519 ± 0.034	1.908PheThr: 1.908 ± 0.034	2.684PheVal: 2.684 ± 0.033	0.437PheTrp: 0.437 ± 0.017	0.635PheTyr: 0.635 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	10.544GlyAla: 10.544 ± 0.098	0.794GlyCys: 0.794 ± 0.025	5.607GlyAsp: 5.607 ± 0.066	4.693GlyGlu: 4.693 ± 0.054	2.994GlyPhe: 2.994 ± 0.046	8.13GlyGly: 8.13 ± 0.098	2.117GlyHis: 2.117 ± 0.038	3.869GlyIle: 3.869 ± 0.061	2.167GlyLys: 2.167 ± 0.045	9.486GlyLeu: 9.486 ± 0.081	2.005GlyMet: 2.005 ± 0.035	1.76GlyAsn: 1.76 ± 0.036	4.494GlyPro: 4.494 ± 0.061	2.642GlyGln: 2.642 ± 0.042	6.873GlyArg: 6.873 ± 0.068	5.607GlySer: 5.607 ± 0.069	6.128GlyThr: 6.128 ± 0.089	8.163GlyVal: 8.163 ± 0.086	1.766GlyTrp: 1.766 ± 0.035	2.28GlyTyr: 2.28 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.469HisAla: 2.469 ± 0.043	0.179HisCys: 0.179 ± 0.01	1.562HisAsp: 1.562 ± 0.036	1.24HisGlu: 1.24 ± 0.03	0.604HisPhe: 0.604 ± 0.018	2.215HisGly: 2.215 ± 0.036	0.69HisHis: 0.69 ± 0.022	0.557HisIle: 0.557 ± 0.02	0.285HisLys: 0.285 ± 0.013	2.413HisLeu: 2.413 ± 0.038	0.286HisMet: 0.286 ± 0.014	0.321HisAsn: 0.321 ± 0.016	1.581HisPro: 1.581 ± 0.035	0.665HisGln: 0.665 ± 0.022	1.843HisArg: 1.843 ± 0.04	0.839HisSer: 0.839 ± 0.022	1.107HisThr: 1.107 ± 0.029	1.927HisVal: 1.927 ± 0.036	0.338HisTrp: 0.338 ± 0.015	0.463HisTyr: 0.463 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	5.289IleAla: 5.289 ± 0.074	0.276IleCys: 0.276 ± 0.013	2.75IleAsp: 2.75 ± 0.039	2.302IleGlu: 2.302 ± 0.042	0.905IlePhe: 0.905 ± 0.027	4.042IleGly: 4.042 ± 0.058	0.638IleHis: 0.638 ± 0.019	1.062IleIle: 1.062 ± 0.031	0.763IleLys: 0.763 ± 0.023	2.667IleLeu: 2.667 ± 0.047	0.452IleMet: 0.452 ± 0.015	0.798IleAsn: 0.798 ± 0.023	1.807IlePro: 1.807 ± 0.033	0.834IleGln: 0.834 ± 0.025	2.246IleArg: 2.246 ± 0.038	2.011IleSer: 2.011 ± 0.036	2.383IleThr: 2.383 ± 0.044	3.33IleVal: 3.33 ± 0.05	0.453IleTrp: 0.453 ± 0.02	0.653IleTyr: 0.653 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	2.574LysAla: 2.574 ± 0.047	0.09LysCys: 0.09 ± 0.008	1.169LysAsp: 1.169 ± 0.028	0.929LysGlu: 0.929 ± 0.027	0.478LysPhe: 0.478 ± 0.018	1.551LysGly: 1.551 ± 0.032	0.436LysHis: 0.436 ± 0.017	0.85LysIle: 0.85 ± 0.026	0.761LysLys: 0.761 ± 0.031	1.723LysLeu: 1.723 ± 0.034	0.368LysMet: 0.368 ± 0.014	0.447LysAsn: 0.447 ± 0.018	1.254LysPro: 1.254 ± 0.038	0.697LysGln: 0.697 ± 0.023	1.318LysArg: 1.318 ± 0.032	1.113LysSer: 1.113 ± 0.031	1.216LysThr: 1.216 ± 0.031	2.085LysVal: 2.085 ± 0.044	0.216LysTrp: 0.216 ± 0.011	0.424LysTyr: 0.424 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	14.637LeuAla: 14.637 ± 0.122	0.708LeuCys: 0.708 ± 0.023	7.384LeuAsp: 7.384 ± 0.081	5.216LeuGlu: 5.216 ± 0.069	2.453LeuPhe: 2.453 ± 0.042	9.649LeuGly: 9.649 ± 0.09	2.034LeuHis: 2.034 ± 0.041	3.016LeuIle: 3.016 ± 0.052	1.692LeuLys: 1.692 ± 0.037	10.51LeuLeu: 10.51 ± 0.118	1.673LeuMet: 1.673 ± 0.033	1.596LeuAsn: 1.596 ± 0.034	5.684LeuPro: 5.684 ± 0.061	2.321LeuGln: 2.321 ± 0.039	7.462LeuArg: 7.462 ± 0.088	5.444LeuSer: 5.444 ± 0.058	6.662LeuThr: 6.662 ± 0.06	10.597LeuVal: 10.597 ± 0.105	1.213LeuTrp: 1.213 ± 0.031	1.6LeuTyr: 1.6 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.21MetAla: 2.21 ± 0.043	0.138MetCys: 0.138 ± 0.009	0.942MetAsp: 0.942 ± 0.025	0.749MetGlu: 0.749 ± 0.026	0.49MetPhe: 0.49 ± 0.019	1.375MetGly: 1.375 ± 0.028	0.379MetHis: 0.379 ± 0.017	0.729MetIle: 0.729 ± 0.024	0.444MetLys: 0.444 ± 0.017	1.853MetLeu: 1.853 ± 0.037	0.353MetMet: 0.353 ± 0.015	0.41MetAsn: 0.41 ± 0.015	1.114MetPro: 1.114 ± 0.027	0.475MetGln: 0.475 ± 0.016	1.424MetArg: 1.424 ± 0.029	1.518MetSer: 1.518 ± 0.029	1.777MetThr: 1.777 ± 0.033	1.54MetVal: 1.54 ± 0.033	0.206MetTrp: 0.206 ± 0.011	0.29MetTyr: 0.29 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.262AsnAla: 2.262 ± 0.041	0.136AsnCys: 0.136 ± 0.009	1.107AsnAsp: 1.107 ± 0.026	0.837AsnGlu: 0.837 ± 0.024	0.473AsnPhe: 0.473 ± 0.019	1.769AsnGly: 1.769 ± 0.039	0.383AsnHis: 0.383 ± 0.015	0.664AsnIle: 0.664 ± 0.021	0.375AsnLys: 0.375 ± 0.016	1.851AsnLeu: 1.851 ± 0.038	0.286AsnMet: 0.286 ± 0.014	0.438AsnAsn: 0.438 ± 0.017	1.387AsnPro: 1.387 ± 0.029	0.588AsnGln: 0.588 ± 0.021	1.222AsnArg: 1.222 ± 0.028	0.808AsnSer: 0.808 ± 0.026	1.049AsnThr: 1.049 ± 0.03	1.543AsnVal: 1.543 ± 0.035	0.259AsnTrp: 0.259 ± 0.012	0.407AsnTyr: 0.407 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.223ProAla: 7.223 ± 0.082	0.269ProCys: 0.269 ± 0.011	4.561ProAsp: 4.561 ± 0.057	3.772ProGlu: 3.772 ± 0.057	1.522ProPhe: 1.522 ± 0.03	5.598ProGly: 5.598 ± 0.069	1.125ProHis: 1.125 ± 0.026	1.759ProIle: 1.759 ± 0.034	1.046ProLys: 1.046 ± 0.029	4.73ProLeu: 4.73 ± 0.052	1.093ProMet: 1.093 ± 0.025	0.879ProAsn: 0.879 ± 0.023	2.812ProPro: 2.812 ± 0.052	1.428ProGln: 1.428 ± 0.026	3.361ProArg: 3.361 ± 0.048	3.152ProSer: 3.152 ± 0.052	3.825ProThr: 3.825 ± 0.058	5.191ProVal: 5.191 ± 0.062	0.921ProTrp: 0.921 ± 0.024	1.22ProTyr: 1.22 ± 0.031	0.001ProXaa: 0.001 ± 0.001
Gln	3.507GlnAla: 3.507 ± 0.054	0.161GlnCys: 0.161 ± 0.009	1.4GlnAsp: 1.4 ± 0.028	1.42GlnGlu: 1.42 ± 0.031	0.736GlnPhe: 0.736 ± 0.023	2.113GlnGly: 2.113 ± 0.034	0.7GlnHis: 0.7 ± 0.021	1.099GlnIle: 1.099 ± 0.023	0.588GlnLys: 0.588 ± 0.018	3.134GlnLeu: 3.134 ± 0.047	0.558GlnMet: 0.558 ± 0.019	0.446GlnAsn: 0.446 ± 0.016	1.654GlnPro: 1.654 ± 0.036	1.256GlnGln: 1.256 ± 0.035	2.324GlnArg: 2.324 ± 0.04	1.285GlnSer: 1.285 ± 0.031	1.525GlnThr: 1.525 ± 0.031	3.019GlnVal: 3.019 ± 0.045	0.443GlnTrp: 0.443 ± 0.017	0.53GlnTyr: 0.53 ± 0.018	0.001GlnXaa: 0.001 ± 0.001
Arg	8.517ArgAla: 8.517 ± 0.088	0.475ArgCys: 0.475 ± 0.017	4.441ArgAsp: 4.441 ± 0.061	4.136ArgGlu: 4.136 ± 0.056	2.18ArgPhe: 2.18 ± 0.036	5.33ArgGly: 5.33 ± 0.057	1.735ArgHis: 1.735 ± 0.03	3.075ArgIle: 3.075 ± 0.041	1.401ArgLys: 1.401 ± 0.031	7.857ArgLeu: 7.857 ± 0.079	1.675ArgMet: 1.675 ± 0.034	1.267ArgAsn: 1.267 ± 0.027	4.043ArgPro: 4.043 ± 0.057	2.074ArgGln: 2.074 ± 0.04	6.819ArgArg: 6.819 ± 0.099	4.158ArgSer: 4.158 ± 0.051	4.807ArgThr: 4.807 ± 0.063	6.242ArgVal: 6.242 ± 0.066	1.324ArgTrp: 1.324 ± 0.031	1.576ArgTyr: 1.576 ± 0.031	0.001ArgXaa: 0.001 ± 0.001
Ser	6.689SerAla: 6.689 ± 0.073	0.374SerCys: 0.374 ± 0.016	3.264SerAsp: 3.264 ± 0.043	2.516SerGlu: 2.516 ± 0.046	1.756SerPhe: 1.756 ± 0.031	5.873SerGly: 5.873 ± 0.072	1.005SerHis: 1.005 ± 0.024	2.0SerIle: 2.0 ± 0.04	1.043SerLys: 1.043 ± 0.032	4.995SerLeu: 4.995 ± 0.064	1.288SerMet: 1.288 ± 0.027	1.009SerAsn: 1.009 ± 0.026	3.054SerPro: 3.054 ± 0.051	1.374SerGln: 1.374 ± 0.03	3.61SerArg: 3.61 ± 0.047	3.334SerSer: 3.334 ± 0.055	3.771SerThr: 3.771 ± 0.054	4.666SerVal: 4.666 ± 0.064	1.016SerTrp: 1.016 ± 0.025	1.45SerTyr: 1.45 ± 0.038	0.001SerXaa: 0.001 ± 0.001
Thr	7.907ThrAla: 7.907 ± 0.097	0.483ThrCys: 0.483 ± 0.019	3.985ThrAsp: 3.985 ± 0.058	3.059ThrGlu: 3.059 ± 0.045	1.969ThrPhe: 1.969 ± 0.039	6.347ThrGly: 6.347 ± 0.08	1.192ThrHis: 1.192 ± 0.028	2.548ThrIle: 2.548 ± 0.042	1.273ThrLys: 1.273 ± 0.03	5.942ThrLeu: 5.942 ± 0.073	1.131ThrMet: 1.131 ± 0.031	1.21ThrAsn: 1.21 ± 0.03	3.962ThrPro: 3.962 ± 0.061	1.587ThrGln: 1.587 ± 0.03	3.855ThrArg: 3.855 ± 0.045	3.852ThrSer: 3.852 ± 0.056	4.583ThrThr: 4.583 ± 0.072	6.219ThrVal: 6.219 ± 0.083	1.153ThrTrp: 1.153 ± 0.029	1.649ThrTyr: 1.649 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	12.841ValAla: 12.841 ± 0.11	0.7ValCys: 0.7 ± 0.022	6.591ValAsp: 6.591 ± 0.08	5.494ValGlu: 5.494 ± 0.069	2.489ValPhe: 2.489 ± 0.039	8.113ValGly: 8.113 ± 0.087	1.968ValHis: 1.968 ± 0.041	3.284ValIle: 3.284 ± 0.056	1.661ValLys: 1.661 ± 0.036	10.016ValLeu: 10.016 ± 0.11	1.531ValMet: 1.531 ± 0.031	1.776ValAsn: 1.776 ± 0.038	5.16ValPro: 5.16 ± 0.067	2.245ValGln: 2.245 ± 0.037	6.939ValArg: 6.939 ± 0.078	4.945ValSer: 4.945 ± 0.063	6.229ValThr: 6.229 ± 0.089	10.869ValVal: 10.869 ± 0.12	1.16ValTrp: 1.16 ± 0.03	1.505ValTyr: 1.505 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.626TrpAla: 1.626 ± 0.032	0.155TrpCys: 0.155 ± 0.01	0.886TrpAsp: 0.886 ± 0.024	0.687TrpGlu: 0.687 ± 0.021	0.556TrpPhe: 0.556 ± 0.019	1.09TrpGly: 1.09 ± 0.027	0.39TrpHis: 0.39 ± 0.015	0.606TrpIle: 0.606 ± 0.02	0.33TrpLys: 0.33 ± 0.015	1.813TrpLeu: 1.813 ± 0.035	0.309TrpMet: 0.309 ± 0.015	0.36TrpAsn: 0.36 ± 0.015	0.768TrpPro: 0.768 ± 0.022	0.541TrpGln: 0.541 ± 0.021	1.294TrpArg: 1.294 ± 0.031	1.083TrpSer: 1.083 ± 0.027	1.079TrpThr: 1.079 ± 0.03	1.379TrpVal: 1.379 ± 0.032	0.391TrpTrp: 0.391 ± 0.015	0.318TrpTyr: 0.318 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.803TyrAla: 2.803 ± 0.041	0.166TyrCys: 0.166 ± 0.01	1.83TyrAsp: 1.83 ± 0.03	1.027TyrGlu: 1.027 ± 0.025	0.659TyrPhe: 0.659 ± 0.021	2.076TyrGly: 2.076 ± 0.036	0.36TyrHis: 0.36 ± 0.017	0.453TyrIle: 0.453 ± 0.018	0.35TyrLys: 0.35 ± 0.016	2.089TyrLeu: 2.089 ± 0.036	0.232TyrMet: 0.232 ± 0.013	0.41TyrAsn: 0.41 ± 0.019	1.042TyrPro: 1.042 ± 0.024	0.598TyrGln: 0.598 ± 0.024	1.552TyrArg: 1.552 ± 0.031	0.993TyrSer: 0.993 ± 0.027	1.103TyrThr: 1.103 ± 0.028	2.043TyrVal: 2.043 ± 0.035	0.35TyrTrp: 0.35 ± 0.016	0.474TyrTyr: 0.474 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 5063 proteins (1661918 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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