Curvibacter phage P26059A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384V795|A0A384V795_9CAUD Thiol reductase thioredoxin OS=Curvibacter phage P26059A OX=1983783 GN=P26059A_0036 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84LGTMSPAAIRR13 pKa = 11.84AVFSPEE19 pKa = 4.0SDD21 pKa = 3.01SDD23 pKa = 4.51LYY25 pKa = 10.91MLLTIYY31 pKa = 10.77DD32 pKa = 3.85PSNPSNVIARR42 pKa = 11.84LSDD45 pKa = 3.85GYY47 pKa = 7.28TQRR50 pKa = 11.84ISEE53 pKa = 4.11TDD55 pKa = 3.28DD56 pKa = 3.42DD57 pKa = 4.27VVYY60 pKa = 10.62GVVSRR65 pKa = 11.84GEE67 pKa = 4.15SFVFLPLNISLPTEE81 pKa = 4.36SDD83 pKa = 3.44DD84 pKa = 4.69QAPTCSITISDD95 pKa = 3.82VTRR98 pKa = 11.84YY99 pKa = 9.86IMPLIRR105 pKa = 11.84QLNGPPSIKK114 pKa = 10.52LEE116 pKa = 4.19LVLSSTPNVVEE127 pKa = 4.33ASFSEE132 pKa = 5.26FYY134 pKa = 10.27LTGITYY140 pKa = 10.22NAEE143 pKa = 4.21SVSGNLSMINYY154 pKa = 9.18DD155 pKa = 3.8LEE157 pKa = 4.46GFPTHH162 pKa = 6.76SFTPFLFPGLFF173 pKa = 3.44

Molecular weight: 19.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384UWT0|A0A384UWT0_9CAUD Uncharacterized protein OS=Curvibacter phage P26059A OX=1983783 GN=P26059A_0019 PE=4 SV=1
MM1 pKa = 7.28YY2 pKa = 10.11LQRR5 pKa = 11.84YY6 pKa = 7.53NPKK9 pKa = 8.71QVYY12 pKa = 8.83VKK14 pKa = 10.36RR15 pKa = 11.84LKK17 pKa = 10.58KK18 pKa = 10.41VLDD21 pKa = 3.86YY22 pKa = 11.17YY23 pKa = 11.16RR24 pKa = 11.84STGCRR29 pKa = 11.84NSRR32 pKa = 11.84EE33 pKa = 3.94YY34 pKa = 11.23AQGKK38 pKa = 9.15SKK40 pKa = 11.13

Molecular weight: 4.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

124

0

124

25026

37

1644

201.8

22.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.703 ± 0.402

1.223 ± 0.144

5.638 ± 0.174

6.234 ± 0.334

3.928 ± 0.144

6.601 ± 0.307

1.918 ± 0.144

5.526 ± 0.144

6.725 ± 0.374

7.896 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.118

5.071 ± 0.17

3.249 ± 0.194

4.248 ± 0.2

4.391 ± 0.164

7.24 ± 0.25

6.537 ± 0.255

7.181 ± 0.222

1.331 ± 0.108

3.936 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski