Salix brachista

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Salix

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N5L4Z0|A0A5N5L4Z0_9ROSI Lipoxygenase domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_015350 PE=4 SV=1
MM1 pKa = 8.43IMEE4 pKa = 4.09VHH6 pKa = 6.37EE7 pKa = 4.73MLFCAPKK14 pKa = 8.97IYY16 pKa = 9.98FYY18 pKa = 11.22EE19 pKa = 4.64FVHH22 pKa = 6.03PQFGGIGCGDD32 pKa = 4.13LEE34 pKa = 4.54EE35 pKa = 4.38VWVTILNITGPLSSWSFADD54 pKa = 5.0NILPDD59 pKa = 3.94PEE61 pKa = 4.48IVDD64 pKa = 4.35GGPPSYY70 pKa = 10.11MLRR73 pKa = 11.84LSGTTQANWTFWLEE87 pKa = 3.96ASSSDD92 pKa = 3.66DD93 pKa = 3.26LRR95 pKa = 11.84VEE97 pKa = 4.23VAVVDD102 pKa = 3.84QVLDD106 pKa = 4.0DD107 pKa = 3.72EE108 pKa = 4.77VEE110 pKa = 4.04RR111 pKa = 11.84LKK113 pKa = 11.45GLFPDD118 pKa = 3.54WADD121 pKa = 3.06VATYY125 pKa = 10.66SSFMSSYY132 pKa = 11.3NFF134 pKa = 3.46

Molecular weight: 15.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N5LYA9|A0A5N5LYA9_9ROSI Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_011038 PE=4 SV=1
MM1 pKa = 7.36GSVVSQAANGIGGVVGNAFAVPIKK25 pKa = 9.75TLFGVSCEE33 pKa = 4.49DD34 pKa = 3.57VCSGPWDD41 pKa = 4.44LICFIEE47 pKa = 4.31HH48 pKa = 6.63LCVSDD53 pKa = 4.26LLKK56 pKa = 11.2LLMIFALSYY65 pKa = 8.15LTLMFFYY72 pKa = 11.16LLFKK76 pKa = 11.01VGICQCIGKK85 pKa = 8.45SLCKK89 pKa = 10.02ICWAGCEE96 pKa = 4.32AYY98 pKa = 9.19WFALEE103 pKa = 6.28DD104 pKa = 3.33ITCFLWHH111 pKa = 6.88KK112 pKa = 10.26LKK114 pKa = 9.24NTKK117 pKa = 9.64RR118 pKa = 11.84VNRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84FRR126 pKa = 11.84DD127 pKa = 2.84IEE129 pKa = 4.14GGYY132 pKa = 8.69TSSSEE137 pKa = 4.08SDD139 pKa = 3.22SSEE142 pKa = 4.43DD143 pKa = 3.44YY144 pKa = 10.91HH145 pKa = 8.77HH146 pKa = 7.47LGRR149 pKa = 11.84KK150 pKa = 8.5NKK152 pKa = 10.36SGMEE156 pKa = 3.85RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84TRR161 pKa = 11.84SRR163 pKa = 11.84SWRR166 pKa = 11.84SLHH169 pKa = 5.88PSSRR173 pKa = 11.84FGSRR177 pKa = 11.84NNHH180 pKa = 5.61HH181 pKa = 6.36RR182 pKa = 11.84HH183 pKa = 4.84HH184 pKa = 6.72VRR186 pKa = 11.84LKK188 pKa = 7.81TRR190 pKa = 11.84EE191 pKa = 3.7ISVHH195 pKa = 4.88VKK197 pKa = 9.95GGSRR201 pKa = 11.84RR202 pKa = 11.84PRR204 pKa = 11.84NSRR207 pKa = 11.84QLQLRR212 pKa = 11.84LRR214 pKa = 11.84NPRR217 pKa = 11.84RR218 pKa = 11.84NMGMFKK224 pKa = 10.38RR225 pKa = 11.84KK226 pKa = 9.6RR227 pKa = 11.84LGG229 pKa = 3.18

Molecular weight: 26.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29803

0

29803

13053624

51

5590

438.0

48.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.727 ± 0.012

1.924 ± 0.006

5.261 ± 0.009

6.44 ± 0.017

4.28 ± 0.009

6.593 ± 0.011

2.378 ± 0.005

5.261 ± 0.009

6.066 ± 0.014

9.784 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.005

4.431 ± 0.008

4.9 ± 0.012

3.69 ± 0.009

5.257 ± 0.01

9.167 ± 0.016

4.846 ± 0.009

6.494 ± 0.01

1.289 ± 0.005

2.746 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski