Dishui Lake phycodnavirus 2
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 188 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XP48|A0A6G6XP48_9PHYC Uncharacterized protein OS=Dishui Lake phycodnavirus 2 OX=2704071 PE=4 SV=1
MM1 pKa = 7.1 VWSQYY6 pKa = 10.35 VYY8 pKa = 10.19 EE9 pKa = 4.66 ASAGLDD15 pKa = 3.01 VDD17 pKa = 4.57 APPAVLSSDD26 pKa = 5.06 DD27 pKa = 3.97 DD28 pKa = 4.67 AADD31 pKa = 3.35 QDD33 pKa = 4.01 VGLNICDD40 pKa = 3.48 WQLKK44 pKa = 9.25 YY45 pKa = 10.71 SEE47 pKa = 4.81 EE48 pKa = 4.06 LWDD51 pKa = 3.55 IWDD54 pKa = 3.91 LMKK57 pKa = 10.36 MLIRR61 pKa = 11.84 DD62 pKa = 3.72 AFLEE66 pKa = 4.39 HH67 pKa = 6.64 TLLTHH72 pKa = 7.75 DD73 pKa = 3.75 YY74 pKa = 8.72 CTYY77 pKa = 10.1 NDD79 pKa = 3.76 FVEE82 pKa = 5.16 FCYY85 pKa = 10.36 YY86 pKa = 10.77 DD87 pKa = 3.08 HH88 pKa = 7.05 YY89 pKa = 11.54 GVEE92 pKa = 4.95 EE93 pKa = 4.15 YY94 pKa = 10.12 TGPVPFRR101 pKa = 11.84 EE102 pKa = 3.89 NLRR105 pKa = 11.84 YY106 pKa = 9.76 IWMVVWNEE114 pKa = 3.66 MKK116 pKa = 10.91 YY117 pKa = 11.06 LDD119 pKa = 4.48 FAPGANFSHH128 pKa = 6.68 FVQWVVEE135 pKa = 3.97 HH136 pKa = 6.78 SEE138 pKa = 4.17 INNLTLL144 pKa = 4.26
Molecular weight: 17.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.911
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.745
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.859
Rodwell 3.783
Grimsley 3.656
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.266
Thurlkill 3.795
EMBOSS 3.872
Sillero 4.075
Patrickios 0.235
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|A0A6G6XNR6|A0A6G6XNR6_9PHYC Uncharacterized protein OS=Dishui Lake phycodnavirus 2 OX=2704071 PE=4 SV=1
MM1 pKa = 8.14 IEE3 pKa = 3.95 VKK5 pKa = 10.42 LIKK8 pKa = 10.55 SPSPKK13 pKa = 9.86 HH14 pKa = 5.58 KK15 pKa = 10.01 FRR17 pKa = 11.84 VIFRR21 pKa = 11.84 TGRR24 pKa = 11.84 YY25 pKa = 8.0 VDD27 pKa = 4.03 FGGRR31 pKa = 11.84 GYY33 pKa = 11.19 SDD35 pKa = 3.44 YY36 pKa = 10.35 TIHH39 pKa = 6.35 KK40 pKa = 7.79 TPEE43 pKa = 3.44 RR44 pKa = 11.84 MRR46 pKa = 11.84 LYY48 pKa = 10.5 VLRR51 pKa = 11.84 HH52 pKa = 5.68 GGRR55 pKa = 11.84 VPSGLWKK62 pKa = 8.21 EE63 pKa = 4.26 TRR65 pKa = 11.84 RR66 pKa = 11.84 TVIQRR71 pKa = 11.84 GMLSVNTSRR80 pKa = 11.84 KK81 pKa = 8.99 EE82 pKa = 3.75 LWTGAGVGTAGFWSRR97 pKa = 11.84 WLLWSYY103 pKa = 11.17 PSLDD107 pKa = 3.65 DD108 pKa = 3.89 AKK110 pKa = 11.21 KK111 pKa = 9.55 FMSRR115 pKa = 11.84 KK116 pKa = 8.88 FQIKK120 pKa = 7.77 FTNVV124 pKa = 2.36
Molecular weight: 14.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.287
IPC2_protein 10.116
IPC_protein 11.067
Toseland 11.184
ProMoST 11.184
Dawson 11.228
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.403
Grimsley 11.272
Solomon 11.491
Lehninger 11.433
Nozaki 11.155
DTASelect 11.038
Thurlkill 11.169
EMBOSS 11.608
Sillero 11.184
Patrickios 11.14
IPC_peptide 11.491
IPC2_peptide 10.131
IPC2.peptide.svr19 8.371
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
188
0
188
51000
54
2586
271.3
30.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.637 ± 0.373
1.739 ± 0.162
5.804 ± 0.198
6.131 ± 0.218
4.431 ± 0.166
6.324 ± 0.296
2.167 ± 0.113
5.649 ± 0.193
6.653 ± 0.421
8.224 ± 0.19
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.763 ± 0.139
5.608 ± 0.392
4.133 ± 0.172
3.52 ± 0.108
4.875 ± 0.213
6.094 ± 0.227
6.557 ± 0.313
7.308 ± 0.184
1.163 ± 0.078
4.222 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here