Stenotrophomonas daejeonensis
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2805 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R0EC49|A0A0R0EC49_9GAMM Glucose dehydrogenase OS=Stenotrophomonas daejeonensis OX=659018 GN=ABB34_00055 PE=4 SV=1
MM1 pKa = 7.73 SDD3 pKa = 3.12 ASASTFRR10 pKa = 11.84 TWMCVVCGFIYY21 pKa = 10.55 SEE23 pKa = 4.21 ADD25 pKa = 3.27 GLPEE29 pKa = 3.94 EE30 pKa = 5.59 GIAAGTRR37 pKa = 11.84 WADD40 pKa = 3.95 VPDD43 pKa = 3.6 TWTCPDD49 pKa = 3.84 CGATKK54 pKa = 10.71 DD55 pKa = 3.72 DD56 pKa = 4.2 FEE58 pKa = 4.49 MVEE61 pKa = 4.23 LDD63 pKa = 3.29
Molecular weight: 6.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 3.63
IPC_protein 3.554
Toseland 3.35
ProMoST 3.668
Dawson 3.567
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.389
Grimsley 3.274
Solomon 3.516
Lehninger 3.478
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.541
Sillero 3.681
Patrickios 0.604
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A0R0DXW9|A0A0R0DXW9_9GAMM Histidinol dehydrogenase OS=Stenotrophomonas daejeonensis OX=659018 GN=hisD PE=3 SV=1
MM1 pKa = 6.79 NTVTQGLDD9 pKa = 3.09 RR10 pKa = 11.84 PASPRR15 pKa = 11.84 LPGLLLAAAIAGLSAWLGNLPPLQAHH41 pKa = 7.47 GIGTLTLAIALGIAAGNAFPDD62 pKa = 4.02 ALSSRR67 pKa = 11.84 ASAGVDD73 pKa = 3.91 FAKK76 pKa = 9.87 GTLLRR81 pKa = 11.84 AGIVLYY87 pKa = 9.89 GLRR90 pKa = 11.84 LTLQDD95 pKa = 3.42 VAQVGLRR102 pKa = 11.84 GVLVDD107 pKa = 5.05 ALVLCSTFALACWLGCRR124 pKa = 11.84 VLKK127 pKa = 10.69 LEE129 pKa = 4.94 RR130 pKa = 11.84 GTALLIGAGSSICGAAAVMATAPVVKK156 pKa = 10.34 GRR158 pKa = 11.84 AEE160 pKa = 3.82 QAAVAVATVVVFGTLAMFAYY180 pKa = 9.42 PLLYY184 pKa = 9.98 RR185 pKa = 11.84 WGVGAGWMPLDD196 pKa = 3.6 EE197 pKa = 5.97 AGFGVFTGATVHH209 pKa = 6.47 EE210 pKa = 4.72 VAQVVAAGRR219 pKa = 11.84 AIGPQAADD227 pKa = 3.05 AAVIAKK233 pKa = 9.21 LVRR236 pKa = 11.84 VMMLAPFLLGLSLLLARR253 pKa = 11.84 GRR255 pKa = 11.84 KK256 pKa = 6.9 TVDD259 pKa = 3.13 GPRR262 pKa = 11.84 PAITVPWFAFGFIGMVAFNSLRR284 pKa = 11.84 LLPAPLVEE292 pKa = 4.57 ALVGLDD298 pKa = 3.4 TALLAMAMAALGLTTRR314 pKa = 11.84 ASALRR319 pKa = 11.84 RR320 pKa = 11.84 AGPRR324 pKa = 11.84 PMLLAALLFAWLLGGGMLINLAIHH348 pKa = 7.02 ALPP351 pKa = 4.46
Molecular weight: 35.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.458
IPC_protein 10.335
Toseland 10.526
ProMoST 10.277
Dawson 10.628
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 10.716
Grimsley 10.687
Solomon 10.774
Lehninger 10.73
Nozaki 10.555
DTASelect 10.365
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.584
Patrickios 10.452
IPC_peptide 10.774
IPC2_peptide 9.692
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2805
0
2805
929062
36
2910
331.2
35.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.18 ± 0.066
0.812 ± 0.015
5.801 ± 0.035
5.431 ± 0.041
3.347 ± 0.028
8.784 ± 0.044
2.297 ± 0.026
3.971 ± 0.035
2.701 ± 0.041
11.201 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.223 ± 0.024
2.542 ± 0.029
5.333 ± 0.035
3.974 ± 0.03
7.689 ± 0.048
4.807 ± 0.035
4.618 ± 0.035
7.452 ± 0.037
1.578 ± 0.021
2.259 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here