Candidatus Syntrophoarchaeum caldarius
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1784 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1F2PBC4|A0A1F2PBC4_9EURY Ribonuclease P protein component 4 OS=Candidatus Syntrophoarchaeum caldarius OX=1838285 GN=rnp4 PE=3 SV=1
MM1 pKa = 7.23 SVKK4 pKa = 10.32 SGEE7 pKa = 4.23 SSTITVTVTSEE18 pKa = 3.87 GNTIADD24 pKa = 3.67 ANVILSSTDD33 pKa = 3.83 GIFSPDD39 pKa = 3.59 SGITDD44 pKa = 3.38 AGGIFTAIYY53 pKa = 7.04 TAPSVSSDD61 pKa = 2.78 ATYY64 pKa = 10.76 AIAVDD69 pKa = 3.99 VTKK72 pKa = 10.8 VGYY75 pKa = 7.29 TQGSSSIDD83 pKa = 3.02 ITVEE87 pKa = 3.43 ADD89 pKa = 3.05 EE90 pKa = 5.12 GEE92 pKa = 3.98 KK93 pKa = 10.96 GMRR96 pKa = 11.84 DD97 pKa = 2.91 NYY99 pKa = 10.58 YY100 pKa = 10.34 RR101 pKa = 11.84 GGGAPRR107 pKa = 11.84 DD108 pKa = 3.89 SDD110 pKa = 3.57 GDD112 pKa = 4.05 GYY114 pKa = 11.64 SDD116 pKa = 3.68 IQEE119 pKa = 4.58 MIAGTDD125 pKa = 3.38 PDD127 pKa = 5.19 DD128 pKa = 4.27 PSSYY132 pKa = 10.42 PGVLPEE138 pKa = 4.72 TPTPTITATPAPSPATITTATPTPTPTQTPTATPTSTPGQPIPISWVVIGIILLVLLGIIAAYY201 pKa = 9.64 IYY203 pKa = 9.99 IQRR206 pKa = 11.84 RR207 pKa = 3.33
Molecular weight: 21.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A1F2PDB9|A0A1F2PDB9_9EURY Metal-dependent phosphoesterase OS=Candidatus Syntrophoarchaeum caldarius OX=1838285 GN=SCAL_000312 PE=4 SV=1
MM1 pKa = 7.16 YY2 pKa = 10.23 NRR4 pKa = 11.84 SAVEE8 pKa = 4.74 RR9 pKa = 11.84 FNAWIEE15 pKa = 4.12 SFKK18 pKa = 10.82 RR19 pKa = 11.84 ILVRR23 pKa = 11.84 FEE25 pKa = 3.86 RR26 pKa = 11.84 LAVCFMAAVNLACIRR41 pKa = 11.84 MVLRR45 pKa = 11.84 VLKK48 pKa = 10.75
Molecular weight: 5.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.677
IPC_protein 10.804
Toseland 10.935
ProMoST 11.052
Dawson 10.979
Bjellqvist 10.818
Wikipedia 11.301
Rodwell 10.994
Grimsley 11.023
Solomon 11.257
Lehninger 11.199
Nozaki 10.935
DTASelect 10.804
Thurlkill 10.921
EMBOSS 11.374
Sillero 10.95
Patrickios 10.833
IPC_peptide 11.272
IPC2_peptide 10.248
IPC2.peptide.svr19 8.834
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1784
0
1784
510543
34
2615
286.2
31.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.227 ± 0.068
1.246 ± 0.029
6.205 ± 0.046
8.02 ± 0.069
3.907 ± 0.04
7.691 ± 0.047
1.777 ± 0.026
8.694 ± 0.056
5.866 ± 0.07
9.312 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.031
3.514 ± 0.07
3.914 ± 0.032
1.937 ± 0.023
5.525 ± 0.056
5.749 ± 0.055
5.196 ± 0.067
7.304 ± 0.044
0.924 ± 0.021
3.228 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here