Pararhodobacter marinus
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4216 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2CIX1|A0A2U2CIX1_9RHOB Lytic murein transglycosylase OS=Pararhodobacter marinus OX=2184063 GN=C4N9_02075 PE=4 SV=1
MM1 pKa = 7.55 APDD4 pKa = 5.67 GYY6 pKa = 10.75 QLSDD10 pKa = 3.29 MPLTAEE16 pKa = 4.5 HH17 pKa = 6.91 LLDD20 pKa = 3.54 ATIYY24 pKa = 10.77 GPTGDD29 pKa = 5.24 AIGDD33 pKa = 3.67 VHH35 pKa = 8.33 DD36 pKa = 6.03 LILGEE41 pKa = 4.81 AGHH44 pKa = 7.23 SDD46 pKa = 3.76 MQHH49 pKa = 6.48 DD50 pKa = 3.69 PAMAEE55 pKa = 4.02 GAMDD59 pKa = 4.68 GATSEE64 pKa = 4.04 DD65 pKa = 3.54 MAMGEE70 pKa = 4.53 TEE72 pKa = 4.31 GAMTDD77 pKa = 3.74 PVDD80 pKa = 3.67 QTEE83 pKa = 4.5 TGASAGVEE91 pKa = 4.08 MTNPPDD97 pKa = 3.27 MDD99 pKa = 4.84 AEE101 pKa = 4.16 NTMDD105 pKa = 4.78 DD106 pKa = 3.96 EE107 pKa = 4.88 PRR109 pKa = 11.84 AADD112 pKa = 3.75 LGEE115 pKa = 4.28 TATTATPGHH124 pKa = 6.68 GGQDD128 pKa = 3.44 GAEE131 pKa = 4.24 TLSHH135 pKa = 7.43 AILDD139 pKa = 3.49 IGGFLGLGEE148 pKa = 4.59 HH149 pKa = 6.34 RR150 pKa = 11.84 VAVPVSDD157 pKa = 3.57 LQIYY161 pKa = 9.7 QNADD165 pKa = 3.26 DD166 pKa = 4.01 VRR168 pKa = 11.84 VYY170 pKa = 10.73 LPWTEE175 pKa = 4.08 EE176 pKa = 3.77 QLEE179 pKa = 4.17 EE180 pKa = 4.02 LPEE183 pKa = 4.01 YY184 pKa = 11.21 VEE186 pKa = 5.92 GDD188 pKa = 3.05 MATYY192 pKa = 10.18 GRR194 pKa = 11.84 SLTPRR199 pKa = 11.84 NTYY202 pKa = 10.08
Molecular weight: 21.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.681
IPC_protein 3.681
Toseland 3.478
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.808
Patrickios 1.1
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.717
Protein with the highest isoelectric point:
>tr|A0A2U2C5X0|A0A2U2C5X0_9RHOB Sarcosine oxidase subunit gamma OS=Pararhodobacter marinus OX=2184063 GN=C4N9_17985 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.9 KK14 pKa = 9.66 RR15 pKa = 11.84 HH16 pKa = 4.49 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.35 SGQKK29 pKa = 9.48 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4216
0
4216
1328425
24
2108
315.1
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.252 ± 0.063
0.868 ± 0.012
5.947 ± 0.031
5.71 ± 0.036
3.54 ± 0.024
8.964 ± 0.043
2.046 ± 0.021
4.838 ± 0.027
2.429 ± 0.032
10.457 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.775 ± 0.017
2.256 ± 0.018
5.555 ± 0.029
3.104 ± 0.019
7.391 ± 0.037
4.856 ± 0.023
5.325 ± 0.022
7.173 ± 0.026
1.446 ± 0.016
2.068 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here