Acinetobacter phage vB_AbaM_B09_Aci05
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 160 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386KB62|A0A386KB62_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_B09_Aci05 OX=2315458 GN=Aci05_147 PE=4 SV=1
MM1 pKa = 7.71 LYY3 pKa = 10.77 SLVLVVFLSNGSEE16 pKa = 4.49 DD17 pKa = 3.49 RR18 pKa = 11.84 TTLAKK23 pKa = 10.44 NYY25 pKa = 9.72 QDD27 pKa = 3.72 YY28 pKa = 11.42 SCIDD32 pKa = 3.07 SRR34 pKa = 11.84 DD35 pKa = 3.63 VVRR38 pKa = 11.84 SQLDD42 pKa = 3.55 YY43 pKa = 11.67 YY44 pKa = 11.7 NNLEE48 pKa = 4.22 SKK50 pKa = 11.09 GDD52 pKa = 3.58 VEE54 pKa = 5.21 FVDD57 pKa = 5.21 VEE59 pKa = 4.47 CEE61 pKa = 3.86 AQKK64 pKa = 11.33 DD65 pKa = 4.17 FII67 pKa = 5.94
Molecular weight: 7.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.837
IPC2_protein 4.113
IPC_protein 4.012
Toseland 3.808
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.732
Solomon 3.986
Lehninger 3.948
Nozaki 4.126
DTASelect 4.342
Thurlkill 3.872
EMBOSS 3.948
Sillero 4.126
Patrickios 1.952
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.035
Protein with the highest isoelectric point:
>tr|A0A386KC19|A0A386KC19_9CAUD Ribonucleoside-diphosphate reductase OS=Acinetobacter phage vB_AbaM_B09_Aci05 OX=2315458 GN=Aci05_081 PE=3 SV=1
MM1 pKa = 7.96 RR2 pKa = 11.84 NTSIVKK8 pKa = 10.06 RR9 pKa = 11.84 GEE11 pKa = 3.83 FKK13 pKa = 10.96 HH14 pKa = 6.38 RR15 pKa = 11.84 VQLPKK20 pKa = 10.7 GVNEE24 pKa = 4.31 RR25 pKa = 11.84 EE26 pKa = 3.68 KK27 pKa = 11.31 QMFIKK32 pKa = 10.44 FAKK35 pKa = 10.07 SVNQGLFKK43 pKa = 11.06 VV44 pKa = 3.96
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.897
IPC_protein 10.599
Toseland 11.374
ProMoST 11.272
Dawson 11.389
Bjellqvist 11.14
Wikipedia 11.652
Rodwell 11.74
Grimsley 11.418
Solomon 11.637
Lehninger 11.594
Nozaki 11.345
DTASelect 11.14
Thurlkill 11.345
EMBOSS 11.784
Sillero 11.345
Patrickios 11.506
IPC_peptide 11.652
IPC2_peptide 9.97
IPC2.peptide.svr19 8.873
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
160
0
160
30291
37
2223
189.3
21.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.107 ± 0.298
1.258 ± 0.123
6.646 ± 0.171
7.316 ± 0.261
4.404 ± 0.198
6.738 ± 0.145
2.017 ± 0.166
6.523 ± 0.143
7.943 ± 0.266
7.963 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.687 ± 0.136
5.157 ± 0.192
2.899 ± 0.114
3.463 ± 0.163
4.387 ± 0.147
6.309 ± 0.226
5.398 ± 0.25
7.081 ± 0.19
1.4 ± 0.079
4.305 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here