Candidatus Rhabdochlamydia sp. T3358
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1685 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A486XKV8|A0A486XKV8_9BACT Uncharacterized protein OS=Candidatus Rhabdochlamydia sp. T3358 OX=2099795 GN=RHT_00759 PE=4 SV=1
MM1 pKa = 7.49 IGAINSNLRR10 pKa = 11.84 TYY12 pKa = 10.87 GAGPSSEE19 pKa = 4.7 ADD21 pKa = 3.31 TSEE24 pKa = 4.15 STAQTSLHH32 pKa = 6.56 SEE34 pKa = 4.47 DD35 pKa = 3.92 NTDD38 pKa = 3.53 ATNQSNEE45 pKa = 4.04 TNTEE49 pKa = 3.5 VDD51 pKa = 3.57 NQTCSYY57 pKa = 10.48 NDD59 pKa = 3.88 SITNNDD65 pKa = 3.62 YY66 pKa = 11.1 GCQHH70 pKa = 6.63 NGQAGVSAWDD80 pKa = 3.38 IAEE83 pKa = 4.68 HH84 pKa = 6.38 IGNGEE89 pKa = 3.89 WGSAARR95 pKa = 11.84 DD96 pKa = 3.63 VFEE99 pKa = 5.82 SSDD102 pKa = 3.51 TTGSNNSDD110 pKa = 2.93 NTTADD115 pKa = 3.33 NASATEE121 pKa = 4.06 KK122 pKa = 11.01
Molecular weight: 12.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A486XL47|A0A486XL47_9BACT Oligopeptide-binding protein OppA OS=Candidatus Rhabdochlamydia sp. T3358 OX=2099795 GN=oppA_5 PE=4 SV=1
MM1 pKa = 6.91 VKK3 pKa = 10.24 KK4 pKa = 9.57 RR5 pKa = 11.84 TTKK8 pKa = 9.36 KK9 pKa = 8.2 TGARR13 pKa = 11.84 KK14 pKa = 6.71 TAKK17 pKa = 8.97 RR18 pKa = 11.84 TARR21 pKa = 11.84 KK22 pKa = 7.42 TGARR26 pKa = 11.84 KK27 pKa = 6.09 TTAKK31 pKa = 9.29 RR32 pKa = 11.84 TVRR35 pKa = 11.84 KK36 pKa = 7.27 TARR39 pKa = 11.84 KK40 pKa = 6.02 TARR43 pKa = 11.84 RR44 pKa = 11.84 TTKK47 pKa = 10.2 RR48 pKa = 11.84 ATARR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 9.63
Molecular weight: 6.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.518
IPC2_protein 11.359
IPC_protein 12.925
Toseland 13.1
ProMoST 13.583
Dawson 13.1
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.896
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.618
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1685
0
1685
544409
29
2825
323.1
36.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.627 ± 0.05
1.499 ± 0.019
4.521 ± 0.039
6.617 ± 0.06
5.105 ± 0.054
5.519 ± 0.079
2.374 ± 0.027
7.888 ± 0.049
7.412 ± 0.061
11.26 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.108 ± 0.025
4.31 ± 0.047
4.039 ± 0.039
4.595 ± 0.039
4.149 ± 0.044
7.074 ± 0.051
5.0 ± 0.047
5.353 ± 0.043
1.145 ± 0.023
3.406 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here