Synechococcus phage S-CBS3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4YCT0|F4YCT0_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBS3 OX=753085 GN=S-CBS3_gp17 PE=4 SV=1
MM1 pKa = 7.53SLNISDD7 pKa = 4.75PLSLLAYY14 pKa = 10.09QSGLADD20 pKa = 3.98PPLVEE25 pKa = 5.11AAAQAADD32 pKa = 4.85DD33 pKa = 3.97LTAQQRR39 pKa = 11.84AYY41 pKa = 10.02KK42 pKa = 10.19IGDD45 pKa = 3.4PVPIVFCRR53 pKa = 11.84RR54 pKa = 11.84VSGNGGVMVAPGATEE69 pKa = 4.3ARR71 pKa = 11.84YY72 pKa = 10.14QNDD75 pKa = 3.42GTTNALTVSTMVVLSEE91 pKa = 4.67GEE93 pKa = 4.03LPQIDD98 pKa = 4.6LADD101 pKa = 4.16CFVGPCRR108 pKa = 11.84QGTWNQTYY116 pKa = 10.47DD117 pKa = 3.39RR118 pKa = 11.84RR119 pKa = 11.84AGTWTPGNFVTTVADD134 pKa = 3.9KK135 pKa = 11.19DD136 pKa = 3.72PWSCPYY142 pKa = 10.85YY143 pKa = 10.36CGTSGRR149 pKa = 11.84YY150 pKa = 8.93EE151 pKa = 4.65DD152 pKa = 4.48MTTMSCVNTFVDD164 pKa = 5.23GSEE167 pKa = 3.88RR168 pKa = 11.84WEE170 pKa = 4.19HH171 pKa = 4.92QLHH174 pKa = 5.17VFVRR178 pKa = 11.84EE179 pKa = 4.89GIQITRR185 pKa = 11.84IIDD188 pKa = 3.63STLGPSNNVIDD199 pKa = 4.17LALYY203 pKa = 11.15LMDD206 pKa = 3.96KK207 pKa = 10.28SGRR210 pKa = 11.84IPSTLIDD217 pKa = 3.86STQMLAAANFTEE229 pKa = 4.54TNGLHH234 pKa = 6.16FNGVFQEE241 pKa = 4.1SLNLDD246 pKa = 3.16EE247 pKa = 4.86WLEE250 pKa = 4.12QISNDD255 pKa = 3.26YY256 pKa = 10.91LLRR259 pKa = 11.84LVEE262 pKa = 4.33LNGKK266 pKa = 9.73FGFRR270 pKa = 11.84PRR272 pKa = 11.84LPVNVDD278 pKa = 2.96HH279 pKa = 7.06TIKK282 pKa = 8.14TTAIDD287 pKa = 3.07WDD289 pKa = 4.02YY290 pKa = 11.2TFTEE294 pKa = 4.39DD295 pKa = 5.18HH296 pKa = 6.81LLPDD300 pKa = 4.08GFEE303 pKa = 3.71IQYY306 pKa = 10.4IPLADD311 pKa = 5.69RR312 pKa = 11.84IPVTLQMMWRR322 pKa = 11.84QQPEE326 pKa = 3.8SDD328 pKa = 2.83IGFPRR333 pKa = 11.84TTEE336 pKa = 3.33ISYY339 pKa = 10.68SGEE342 pKa = 3.8AADD345 pKa = 6.44GPFEE349 pKa = 4.29QYY351 pKa = 10.91DD352 pKa = 3.56LSGYY356 pKa = 7.51CTSEE360 pKa = 3.57THH362 pKa = 5.58AVKK365 pKa = 10.75VGAYY369 pKa = 9.28RR370 pKa = 11.84LARR373 pKa = 11.84RR374 pKa = 11.84KK375 pKa = 9.96FITHH379 pKa = 5.66TLRR382 pKa = 11.84LKK384 pKa = 10.44VRR386 pKa = 11.84PANYY390 pKa = 10.26NSVLTQGDD398 pKa = 3.62IVRR401 pKa = 11.84VRR403 pKa = 11.84LRR405 pKa = 11.84RR406 pKa = 11.84EE407 pKa = 3.49TALAALDD414 pKa = 3.76YY415 pKa = 11.16HH416 pKa = 6.99DD417 pKa = 5.13FLYY420 pKa = 10.71EE421 pKa = 3.55IEE423 pKa = 5.16RR424 pKa = 11.84IEE426 pKa = 4.09KK427 pKa = 8.06TASGACIFDD436 pKa = 3.79LTHH439 pKa = 6.8FPIDD443 pKa = 3.4AQGRR447 pKa = 11.84SLVALEE453 pKa = 4.29VAAATAPGVVIASGRR468 pKa = 11.84SDD470 pKa = 3.7YY471 pKa = 11.72SCDD474 pKa = 4.17DD475 pKa = 3.56NSSTPGTSVGSGGIDD490 pKa = 3.51FPAFDD495 pKa = 5.0DD496 pKa = 4.54TPGLDD501 pKa = 3.52DD502 pKa = 3.54TTVDD506 pKa = 4.35FNVPTDD512 pKa = 4.28DD513 pKa = 3.19EE514 pKa = 4.68WATGGYY520 pKa = 9.73SPIGPDD526 pKa = 3.13VSQPAGEE533 pKa = 4.14PSGGQTPIGGWDD545 pKa = 3.53NPADD549 pKa = 4.12PLEE552 pKa = 4.46STLDD556 pKa = 3.58QSGVGYY562 pKa = 8.54LTGGTGTGGAPQSGDD577 pKa = 3.51TLSVADD583 pKa = 4.66TDD585 pKa = 4.38FTCAGQVCWSKK596 pKa = 10.82INKK599 pKa = 7.82DD600 pKa = 3.15TGVQTDD606 pKa = 4.01ISCQDD611 pKa = 3.47EE612 pKa = 4.97PISGSWDD619 pKa = 3.27LSITTDD625 pKa = 3.47EE626 pKa = 3.96VDD628 pKa = 3.4HH629 pKa = 6.73FIVATGRR636 pKa = 11.84CKK638 pKa = 10.87DD639 pKa = 3.39PSTASGWGPPQSLGQTAAVLPPAVPSCGDD668 pKa = 3.57LVSVTIKK675 pKa = 10.11WKK677 pKa = 10.0MSIAGYY683 pKa = 8.94TSACVPSTSICSAFGDD699 pKa = 3.74NQYY702 pKa = 11.36SKK704 pKa = 9.82TITSVPRR711 pKa = 11.84GTQIDD716 pKa = 3.72FGGQINAGAGTCADD730 pKa = 4.93KK731 pKa = 11.09INAAISYY738 pKa = 6.97WKK740 pKa = 10.27CIGGVATYY748 pKa = 8.53VTEE751 pKa = 4.3ALGTPGCSYY760 pKa = 10.84NVSAGYY766 pKa = 7.74TQTPYY771 pKa = 10.89DD772 pKa = 3.99YY773 pKa = 10.55EE774 pKa = 4.2AVGDD778 pKa = 3.86VTYY781 pKa = 10.12TGPP784 pKa = 3.79

Molecular weight: 84.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4YCU6|F4YCU6_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBS3 OX=753085 GN=S-CBS3_gp33 PE=4 SV=1
MM1 pKa = 7.75TDD3 pKa = 3.23PVLQEE8 pKa = 3.76LEE10 pKa = 3.87RR11 pKa = 11.84LRR13 pKa = 11.84EE14 pKa = 3.75YY15 pKa = 10.67RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ADD20 pKa = 2.96AKK22 pKa = 10.37RR23 pKa = 11.84RR24 pKa = 11.84DD25 pKa = 3.76RR26 pKa = 11.84QVQARR31 pKa = 11.84LPPKK35 pKa = 10.03LAQQFTAYY43 pKa = 8.64MQAHH47 pKa = 7.23GLNQNQAIVAILYY60 pKa = 7.61TFFNN64 pKa = 4.09

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

9963

40

836

216.6

23.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.312 ± 0.479

1.174 ± 0.194

6.072 ± 0.253

5.37 ± 0.317

3.433 ± 0.259

8.301 ± 0.421

1.787 ± 0.211

5.099 ± 0.279

3.192 ± 0.253

8.15 ± 0.401

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.201

2.901 ± 0.155

5.179 ± 0.312

4.487 ± 0.194

6.243 ± 0.405

6.635 ± 0.306

7.488 ± 0.525

6.213 ± 0.27

1.887 ± 0.172

2.69 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski