Amino acid dipepetide frequency for Synechococcus phage S-CBS3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.057AlaAla: 15.057 ± 2.114
1.004AlaCys: 1.004 ± 0.264
7.529AlaAsp: 7.529 ± 0.831
6.525AlaGlu: 6.525 ± 0.956
4.216AlaPhe: 4.216 ± 0.65
7.529AlaGly: 7.529 ± 1.168
1.205AlaHis: 1.205 ± 0.363
5.822AlaIle: 5.822 ± 0.761
4.015AlaLys: 4.015 ± 0.604
8.231AlaLeu: 8.231 ± 1.143
3.614AlaMet: 3.614 ± 0.624
2.61AlaAsn: 2.61 ± 0.533
5.923AlaPro: 5.923 ± 0.883
4.919AlaGln: 4.919 ± 0.782
6.324AlaArg: 6.324 ± 0.866
7.729AlaSer: 7.729 ± 1.036
8.432AlaThr: 8.432 ± 1.163
7.328AlaVal: 7.328 ± 0.896
1.706AlaTrp: 1.706 ± 0.449
3.413AlaTyr: 3.413 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
1.405CysAla: 1.405 ± 0.362
0.201CysCys: 0.201 ± 0.143
0.703CysAsp: 0.703 ± 0.226
0.402CysGlu: 0.402 ± 0.216
0.301CysPhe: 0.301 ± 0.156
1.104CysGly: 1.104 ± 0.324
0.703CysHis: 0.703 ± 0.234
0.703CysIle: 0.703 ± 0.242
0.301CysLys: 0.301 ± 0.177
0.703CysLeu: 0.703 ± 0.284
0.502CysMet: 0.502 ± 0.221
0.201CysAsn: 0.201 ± 0.143
0.502CysPro: 0.502 ± 0.224
0.201CysGln: 0.201 ± 0.143
1.305CysArg: 1.305 ± 0.345
0.903CysSer: 0.903 ± 0.243
0.602CysThr: 0.602 ± 0.176
0.803CysVal: 0.803 ± 0.286
0.201CysTrp: 0.201 ± 0.109
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.119AspAla: 5.119 ± 0.662
0.903AspCys: 0.903 ± 0.335
3.313AspAsp: 3.313 ± 0.563
2.71AspGlu: 2.71 ± 0.444
3.011AspPhe: 3.011 ± 0.637
5.923AspGly: 5.923 ± 0.807
1.104AspHis: 1.104 ± 0.374
2.008AspIle: 2.008 ± 0.426
1.606AspLys: 1.606 ± 0.398
5.421AspLeu: 5.421 ± 0.767
1.305AspMet: 1.305 ± 0.321
1.606AspAsn: 1.606 ± 0.33
4.919AspPro: 4.919 ± 0.675
2.811AspGln: 2.811 ± 0.516
4.116AspArg: 4.116 ± 0.686
3.313AspSer: 3.313 ± 0.58
4.517AspThr: 4.517 ± 0.826
3.915AspVal: 3.915 ± 0.553
0.903AspTrp: 0.903 ± 0.274
2.208AspTyr: 2.208 ± 0.458
0.0AspXaa: 0.0 ± 0.0
Glu
6.525GluAla: 6.525 ± 0.721
0.502GluCys: 0.502 ± 0.247
2.51GluAsp: 2.51 ± 0.493
1.907GluGlu: 1.907 ± 0.491
1.807GluPhe: 1.807 ± 0.566
4.216GluGly: 4.216 ± 0.671
1.606GluHis: 1.606 ± 0.349
2.811GluIle: 2.811 ± 0.465
1.807GluLys: 1.807 ± 0.346
5.521GluLeu: 5.521 ± 0.687
1.405GluMet: 1.405 ± 0.348
0.402GluAsn: 0.402 ± 0.17
3.714GluPro: 3.714 ± 0.466
2.409GluGln: 2.409 ± 0.358
4.015GluArg: 4.015 ± 0.548
3.313GluSer: 3.313 ± 0.706
3.313GluThr: 3.313 ± 0.682
4.116GluVal: 4.116 ± 0.761
1.004GluTrp: 1.004 ± 0.344
0.803GluTyr: 0.803 ± 0.29
0.0GluXaa: 0.0 ± 0.0
Phe
2.409PheAla: 2.409 ± 0.64
0.803PheCys: 0.803 ± 0.21
2.309PheAsp: 2.309 ± 0.411
2.208PheGlu: 2.208 ± 0.479
0.602PhePhe: 0.602 ± 0.243
3.011PheGly: 3.011 ± 0.467
0.502PheHis: 0.502 ± 0.253
1.706PheIle: 1.706 ± 0.33
0.903PheLys: 0.903 ± 0.288
1.606PheLeu: 1.606 ± 0.375
0.803PheMet: 0.803 ± 0.232
1.305PheAsn: 1.305 ± 0.402
1.405PhePro: 1.405 ± 0.414
1.104PheGln: 1.104 ± 0.378
2.61PheArg: 2.61 ± 0.529
2.71PheSer: 2.71 ± 0.557
4.417PheThr: 4.417 ± 0.771
2.71PheVal: 2.71 ± 0.626
0.502PheTrp: 0.502 ± 0.231
0.703PheTyr: 0.703 ± 0.238
0.0PheXaa: 0.0 ± 0.0
Gly
8.834GlyAla: 8.834 ± 1.427
0.903GlyCys: 0.903 ± 0.29
6.023GlyAsp: 6.023 ± 0.6
3.614GlyGlu: 3.614 ± 0.463
3.212GlyPhe: 3.212 ± 0.625
7.83GlyGly: 7.83 ± 1.255
0.803GlyHis: 0.803 ± 0.218
4.116GlyIle: 4.116 ± 0.522
2.61GlyLys: 2.61 ± 0.507
7.529GlyLeu: 7.529 ± 1.118
1.205GlyMet: 1.205 ± 0.382
3.413GlyAsn: 3.413 ± 0.427
3.112GlyPro: 3.112 ± 0.547
3.413GlyGln: 3.413 ± 0.523
4.316GlyArg: 4.316 ± 0.548
7.127GlySer: 7.127 ± 1.544
5.923GlyThr: 5.923 ± 1.129
4.618GlyVal: 4.618 ± 0.562
1.606GlyTrp: 1.606 ± 0.407
2.811GlyTyr: 2.811 ± 0.599
0.0GlyXaa: 0.0 ± 0.0
His
1.506HisAla: 1.506 ± 0.412
0.201HisCys: 0.201 ± 0.132
0.602HisAsp: 0.602 ± 0.234
1.205HisGlu: 1.205 ± 0.364
1.004HisPhe: 1.004 ± 0.348
1.205HisGly: 1.205 ± 0.387
0.502HisHis: 0.502 ± 0.203
0.703HisIle: 0.703 ± 0.262
0.602HisLys: 0.602 ± 0.205
1.907HisLeu: 1.907 ± 0.5
0.502HisMet: 0.502 ± 0.222
0.402HisAsn: 0.402 ± 0.2
1.004HisPro: 1.004 ± 0.257
1.506HisGln: 1.506 ± 0.421
1.405HisArg: 1.405 ± 0.497
0.703HisSer: 0.703 ± 0.293
1.405HisThr: 1.405 ± 0.389
0.502HisVal: 0.502 ± 0.213
0.402HisTrp: 0.402 ± 0.233
0.602HisTyr: 0.602 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
4.818IleAla: 4.818 ± 0.916
0.502IleCys: 0.502 ± 0.209
4.417IleAsp: 4.417 ± 0.719
3.915IleGlu: 3.915 ± 0.551
1.004IlePhe: 1.004 ± 0.33
4.618IleGly: 4.618 ± 0.615
0.703IleHis: 0.703 ± 0.263
2.51IleIle: 2.51 ± 0.526
1.706IleLys: 1.706 ± 0.302
2.811IleLeu: 2.811 ± 0.533
0.502IleMet: 0.502 ± 0.214
1.807IleAsn: 1.807 ± 0.342
2.008IlePro: 2.008 ± 0.558
2.208IleGln: 2.208 ± 0.425
3.915IleArg: 3.915 ± 0.62
3.313IleSer: 3.313 ± 0.663
5.722IleThr: 5.722 ± 0.831
2.71IleVal: 2.71 ± 0.561
0.803IleTrp: 0.803 ± 0.378
1.004IleTyr: 1.004 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
4.618LysAla: 4.618 ± 0.623
0.201LysCys: 0.201 ± 0.127
2.008LysAsp: 2.008 ± 0.517
1.706LysGlu: 1.706 ± 0.486
1.305LysPhe: 1.305 ± 0.32
2.51LysGly: 2.51 ± 0.851
0.301LysHis: 0.301 ± 0.195
1.706LysIle: 1.706 ± 0.341
0.703LysLys: 0.703 ± 0.284
3.513LysLeu: 3.513 ± 0.886
0.502LysMet: 0.502 ± 0.195
0.703LysAsn: 0.703 ± 0.271
2.108LysPro: 2.108 ± 0.574
1.506LysGln: 1.506 ± 0.33
2.108LysArg: 2.108 ± 0.496
1.205LysSer: 1.205 ± 0.388
2.51LysThr: 2.51 ± 0.52
1.807LysVal: 1.807 ± 0.465
0.602LysTrp: 0.602 ± 0.205
0.301LysTyr: 0.301 ± 0.178
0.0LysXaa: 0.0 ± 0.0
Leu
8.532LeuAla: 8.532 ± 0.974
0.903LeuCys: 0.903 ± 0.32
5.621LeuAsp: 5.621 ± 0.789
4.718LeuGlu: 4.718 ± 0.618
2.309LeuPhe: 2.309 ± 0.528
6.424LeuGly: 6.424 ± 0.951
2.108LeuHis: 2.108 ± 0.426
4.216LeuIle: 4.216 ± 0.712
4.216LeuLys: 4.216 ± 0.829
6.324LeuLeu: 6.324 ± 1.019
2.108LeuMet: 2.108 ± 0.468
2.61LeuAsn: 2.61 ± 0.506
3.313LeuPro: 3.313 ± 0.576
4.919LeuGln: 4.919 ± 0.595
4.618LeuArg: 4.618 ± 0.933
5.22LeuSer: 5.22 ± 0.547
5.421LeuThr: 5.421 ± 0.768
4.718LeuVal: 4.718 ± 0.606
1.606LeuTrp: 1.606 ± 0.315
1.606LeuTyr: 1.606 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
4.015MetAla: 4.015 ± 0.56
0.201MetCys: 0.201 ± 0.144
1.506MetAsp: 1.506 ± 0.301
1.606MetGlu: 1.606 ± 0.561
0.502MetPhe: 0.502 ± 0.255
1.506MetGly: 1.506 ± 0.362
0.301MetHis: 0.301 ± 0.176
0.803MetIle: 0.803 ± 0.302
0.502MetLys: 0.502 ± 0.207
2.409MetLeu: 2.409 ± 0.404
0.602MetMet: 0.602 ± 0.189
1.104MetAsn: 1.104 ± 0.298
2.008MetPro: 2.008 ± 0.436
1.004MetGln: 1.004 ± 0.404
0.803MetArg: 0.803 ± 0.243
0.803MetSer: 0.803 ± 0.319
2.008MetThr: 2.008 ± 0.59
1.506MetVal: 1.506 ± 0.349
0.602MetTrp: 0.602 ± 0.228
0.1MetTyr: 0.1 ± 0.096
0.0MetXaa: 0.0 ± 0.0
Asn
2.61AsnAla: 2.61 ± 0.491
0.1AsnCys: 0.1 ± 0.106
2.008AsnAsp: 2.008 ± 0.311
0.903AsnGlu: 0.903 ± 0.251
0.903AsnPhe: 0.903 ± 0.284
3.112AsnGly: 3.112 ± 0.46
0.502AsnHis: 0.502 ± 0.173
1.305AsnIle: 1.305 ± 0.287
0.803AsnLys: 0.803 ± 0.259
1.907AsnLeu: 1.907 ± 0.404
0.803AsnMet: 0.803 ± 0.316
0.502AsnAsn: 0.502 ± 0.171
1.706AsnPro: 1.706 ± 0.359
1.606AsnGln: 1.606 ± 0.495
1.706AsnArg: 1.706 ± 0.401
2.208AsnSer: 2.208 ± 0.434
2.208AsnThr: 2.208 ± 0.506
2.008AsnVal: 2.008 ± 0.441
1.004AsnTrp: 1.004 ± 0.374
1.104AsnTyr: 1.104 ± 0.308
0.0AsnXaa: 0.0 ± 0.0
Pro
6.826ProAla: 6.826 ± 0.82
0.502ProCys: 0.502 ± 0.197
3.614ProAsp: 3.614 ± 0.591
2.409ProGlu: 2.409 ± 0.47
2.409ProPhe: 2.409 ± 0.462
4.517ProGly: 4.517 ± 0.757
0.903ProHis: 0.903 ± 0.377
2.61ProIle: 2.61 ± 0.608
1.205ProLys: 1.205 ± 0.381
3.814ProLeu: 3.814 ± 0.682
1.305ProMet: 1.305 ± 0.435
1.205ProAsn: 1.205 ± 0.35
1.907ProPro: 1.907 ± 0.587
1.807ProGln: 1.807 ± 0.475
2.309ProArg: 2.309 ± 0.401
3.814ProSer: 3.814 ± 0.63
3.915ProThr: 3.915 ± 0.834
4.216ProVal: 4.216 ± 0.489
1.104ProTrp: 1.104 ± 0.264
1.405ProTyr: 1.405 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
5.22GlnAla: 5.22 ± 0.679
0.301GlnCys: 0.301 ± 0.176
1.807GlnAsp: 1.807 ± 0.385
2.108GlnGlu: 2.108 ± 0.506
1.205GlnPhe: 1.205 ± 0.291
2.61GlnGly: 2.61 ± 0.475
0.803GlnHis: 0.803 ± 0.307
3.614GlnIle: 3.614 ± 0.633
0.703GlnLys: 0.703 ± 0.282
4.417GlnLeu: 4.417 ± 0.909
0.803GlnMet: 0.803 ± 0.333
0.903GlnAsn: 0.903 ± 0.304
3.112GlnPro: 3.112 ± 0.69
3.212GlnGln: 3.212 ± 0.692
3.614GlnArg: 3.614 ± 0.538
2.409GlnSer: 2.409 ± 0.507
3.212GlnThr: 3.212 ± 0.66
2.61GlnVal: 2.61 ± 0.519
1.104GlnTrp: 1.104 ± 0.428
1.104GlnTyr: 1.104 ± 0.341
0.0GlnXaa: 0.0 ± 0.0
Arg
6.826ArgAla: 6.826 ± 0.853
1.004ArgCys: 1.004 ± 0.297
3.313ArgAsp: 3.313 ± 0.6
3.915ArgGlu: 3.915 ± 0.649
1.706ArgPhe: 1.706 ± 0.433
4.618ArgGly: 4.618 ± 0.793
1.606ArgHis: 1.606 ± 0.448
2.51ArgIle: 2.51 ± 0.479
2.51ArgLys: 2.51 ± 0.602
5.822ArgLeu: 5.822 ± 0.623
1.104ArgMet: 1.104 ± 0.338
1.706ArgAsn: 1.706 ± 0.339
3.112ArgPro: 3.112 ± 0.662
3.313ArgGln: 3.313 ± 0.578
5.119ArgArg: 5.119 ± 0.956
3.313ArgSer: 3.313 ± 0.649
3.513ArgThr: 3.513 ± 0.6
3.011ArgVal: 3.011 ± 0.581
1.807ArgTrp: 1.807 ± 0.495
2.51ArgTyr: 2.51 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
6.826SerAla: 6.826 ± 0.885
1.004SerCys: 1.004 ± 0.457
3.413SerAsp: 3.413 ± 0.719
3.313SerGlu: 3.313 ± 0.617
2.409SerPhe: 2.409 ± 0.575
8.633SerGly: 8.633 ± 1.059
1.405SerHis: 1.405 ± 0.333
3.313SerIle: 3.313 ± 0.577
1.907SerLys: 1.907 ± 0.421
4.818SerLeu: 4.818 ± 0.722
1.405SerMet: 1.405 ± 0.33
2.51SerAsn: 2.51 ± 0.388
3.614SerPro: 3.614 ± 0.6
1.706SerGln: 1.706 ± 0.369
3.313SerArg: 3.313 ± 0.694
4.718SerSer: 4.718 ± 0.652
4.818SerThr: 4.818 ± 0.802
4.015SerVal: 4.015 ± 0.862
1.004SerTrp: 1.004 ± 0.258
2.208SerTyr: 2.208 ± 0.484
0.0SerXaa: 0.0 ± 0.0
Thr
9.135ThrAla: 9.135 ± 1.15
0.903ThrCys: 0.903 ± 0.332
3.814ThrAsp: 3.814 ± 0.752
4.116ThrGlu: 4.116 ± 0.766
3.212ThrPhe: 3.212 ± 0.654
6.525ThrGly: 6.525 ± 1.022
1.405ThrHis: 1.405 ± 0.376
4.919ThrIle: 4.919 ± 0.747
2.208ThrLys: 2.208 ± 0.534
5.621ThrLeu: 5.621 ± 0.811
1.506ThrMet: 1.506 ± 0.413
2.208ThrAsn: 2.208 ± 0.43
3.915ThrPro: 3.915 ± 0.62
2.409ThrGln: 2.409 ± 0.465
2.61ThrArg: 2.61 ± 0.52
5.421ThrSer: 5.421 ± 0.661
5.621ThrThr: 5.621 ± 1.03
5.421ThrVal: 5.421 ± 0.556
1.205ThrTrp: 1.205 ± 0.309
2.71ThrTyr: 2.71 ± 0.675
0.0ThrXaa: 0.0 ± 0.0
Val
8.532ValAla: 8.532 ± 1.036
0.703ValCys: 0.703 ± 0.247
3.112ValAsp: 3.112 ± 0.558
3.614ValGlu: 3.614 ± 0.602
1.907ValPhe: 1.907 ± 0.422
4.015ValGly: 4.015 ± 0.48
0.903ValHis: 0.903 ± 0.382
3.915ValIle: 3.915 ± 0.664
1.807ValLys: 1.807 ± 0.388
5.019ValLeu: 5.019 ± 0.844
2.409ValMet: 2.409 ± 0.553
2.108ValAsn: 2.108 ± 0.417
2.61ValPro: 2.61 ± 0.649
2.71ValGln: 2.71 ± 0.445
4.316ValArg: 4.316 ± 0.717
4.116ValSer: 4.116 ± 0.643
5.019ValThr: 5.019 ± 0.71
3.413ValVal: 3.413 ± 0.48
0.903ValTrp: 0.903 ± 0.293
1.004ValTyr: 1.004 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
2.008TrpAla: 2.008 ± 0.453
0.301TrpCys: 0.301 ± 0.177
1.606TrpAsp: 1.606 ± 0.402
0.903TrpGlu: 0.903 ± 0.276
0.502TrpPhe: 0.502 ± 0.195
1.104TrpGly: 1.104 ± 0.275
0.301TrpHis: 0.301 ± 0.164
0.703TrpIle: 0.703 ± 0.283
0.803TrpLys: 0.803 ± 0.242
1.305TrpLeu: 1.305 ± 0.329
0.602TrpMet: 0.602 ± 0.209
0.803TrpAsn: 0.803 ± 0.279
0.803TrpPro: 0.803 ± 0.329
1.004TrpGln: 1.004 ± 0.351
1.807TrpArg: 1.807 ± 0.488
1.506TrpSer: 1.506 ± 0.285
0.703TrpThr: 0.703 ± 0.275
1.205TrpVal: 1.205 ± 0.34
0.301TrpTrp: 0.301 ± 0.182
0.602TrpTyr: 0.602 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.309TyrAla: 2.309 ± 0.542
0.602TyrCys: 0.602 ± 0.257
1.506TyrAsp: 1.506 ± 0.332
1.907TyrGlu: 1.907 ± 0.39
0.803TyrPhe: 0.803 ± 0.283
2.008TyrGly: 2.008 ± 0.481
0.201TyrHis: 0.201 ± 0.122
1.004TyrIle: 1.004 ± 0.275
1.004TyrLys: 1.004 ± 0.312
2.811TyrLeu: 2.811 ± 0.608
0.803TyrMet: 0.803 ± 0.262
0.903TyrAsn: 0.903 ± 0.307
1.004TyrPro: 1.004 ± 0.315
1.104TyrGln: 1.104 ± 0.312
2.108TyrArg: 2.108 ± 0.509
2.51TyrSer: 2.51 ± 0.479
1.606TyrThr: 1.606 ± 0.374
1.506TyrVal: 1.506 ± 0.325
0.502TyrTrp: 0.502 ± 0.191
0.703TyrTyr: 0.703 ± 0.27
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (9963 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski