Etheostoma fonticola aquareovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Aquareovirus; unclassified Aquareovirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193BJ75|A0A193BJ75_9REOV Putative non-structural protein 23 OS=Etheostoma fonticola aquareovirus OX=1862978 PE=4 SV=1
MM1 pKa = 7.55GNVQTSTNVYY11 pKa = 9.81NVNGDD16 pKa = 3.65NNAFTPNSEE25 pKa = 4.32MVASASPAIDD35 pKa = 4.04LKK37 pKa = 11.17PGVLNPNGKK46 pKa = 9.99LYY48 pKa = 10.52QLEE51 pKa = 4.5SGSAPDD57 pKa = 4.0PSNLVIVVDD66 pKa = 3.65ATEE69 pKa = 4.77GDD71 pKa = 3.63FSYY74 pKa = 9.33ITNNTWEE81 pKa = 4.45TLNKK85 pKa = 10.09SALEE89 pKa = 4.21TNSWEE94 pKa = 3.97PLFAVTMTGCGPLKK108 pKa = 10.89LNDD111 pKa = 3.62YY112 pKa = 8.3TVTMSGYY119 pKa = 10.19VGASADD125 pKa = 3.6DD126 pKa = 4.2AFDD129 pKa = 3.97GGVVEE134 pKa = 5.89DD135 pKa = 4.7GMFISSRR142 pKa = 11.84RR143 pKa = 11.84LKK145 pKa = 10.61NFKK148 pKa = 10.69LMLSNRR154 pKa = 11.84CEE156 pKa = 5.54AIAKK160 pKa = 8.46WNTSLNQAMSLLTPDD175 pKa = 4.75LLAGSGSCKK184 pKa = 9.29WKK186 pKa = 10.79SILQYY191 pKa = 9.89MQATLPADD199 pKa = 3.76NEE201 pKa = 4.2VLQYY205 pKa = 10.55PDD207 pKa = 3.01EE208 pKa = 5.04FYY210 pKa = 10.68TVAVSKK216 pKa = 11.08YY217 pKa = 8.51PALKK221 pKa = 10.35PGASPNTPLPVAGPLGEE238 pKa = 4.19IASVMNAASSSVGLMNGSSALLTSAMDD265 pKa = 3.52TLAAKK270 pKa = 10.48NLDD273 pKa = 4.12LVCAEE278 pKa = 4.64APLPVATFAPSLVPRR293 pKa = 11.84DD294 pKa = 3.63YY295 pKa = 11.15RR296 pKa = 11.84PAFIKK301 pKa = 10.8DD302 pKa = 3.57ADD304 pKa = 3.93AHH306 pKa = 6.56WITSVDD312 pKa = 3.17PSAIIRR318 pKa = 11.84VTTALSGRR326 pKa = 11.84DD327 pKa = 3.44YY328 pKa = 10.82TVQLGPGATKK338 pKa = 10.71LIDD341 pKa = 3.44MNRR344 pKa = 11.84MVDD347 pKa = 4.19ADD349 pKa = 4.32LLLDD353 pKa = 3.77VSGMPINWLDD363 pKa = 3.59NPDD366 pKa = 3.52YY367 pKa = 9.62ATAVAAIVLLEE378 pKa = 4.25AKK380 pKa = 10.56VPATEE385 pKa = 4.15INVAADD391 pKa = 3.2ITGVSVVASSPLSIVNSTINVRR413 pKa = 11.84GQSYY417 pKa = 11.07LEE419 pKa = 4.01MLHH422 pKa = 6.76LRR424 pKa = 11.84TTFEE428 pKa = 4.24RR429 pKa = 11.84EE430 pKa = 3.91TIAGKK435 pKa = 9.62PYY437 pKa = 10.07IYY439 pKa = 10.83GFGCLMLLSPTTSSNTRR456 pKa = 11.84NPTLMDD462 pKa = 3.73GLLTVTPILLRR473 pKa = 11.84EE474 pKa = 3.97TTYY477 pKa = 10.6KK478 pKa = 11.06GEE480 pKa = 4.11IVEE483 pKa = 4.99EE484 pKa = 4.14IVPSDD489 pKa = 2.97ILGNHH494 pKa = 6.02TSEE497 pKa = 4.18EE498 pKa = 4.15MAVALANDD506 pKa = 3.71AVFLMEE512 pKa = 4.4NSLKK516 pKa = 10.48EE517 pKa = 3.86VAEE520 pKa = 4.68VIGSAVPIASDD531 pKa = 3.34IDD533 pKa = 4.01DD534 pKa = 3.97SATASVVSRR543 pKa = 11.84LAIAEE548 pKa = 4.44TISMRR553 pKa = 11.84ARR555 pKa = 11.84SGNYY559 pKa = 9.0RR560 pKa = 11.84AFPDD564 pKa = 6.04FGALWKK570 pKa = 9.74KK571 pKa = 10.56AKK573 pKa = 9.93RR574 pKa = 11.84AASLFVSNPKK584 pKa = 10.13SVLQVGVPVLASAGVLDD601 pKa = 4.79ALTSAVGTSVRR612 pKa = 11.84TGNIGKK618 pKa = 9.62GVQDD622 pKa = 3.29AVSILRR628 pKa = 11.84ARR630 pKa = 11.84NSVTKK635 pKa = 10.48LRR637 pKa = 11.84QGFFSKK643 pKa = 10.19IEE645 pKa = 4.03EE646 pKa = 4.39LWPVLDD652 pKa = 4.12GG653 pKa = 4.61

Molecular weight: 69.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193BJ76|A0A193BJ76_9REOV Putative non-structural protein 38 OS=Etheostoma fonticola aquareovirus OX=1862978 PE=4 SV=1
MM1 pKa = 7.71GNTISNTVQYY11 pKa = 9.88TVLQIDD17 pKa = 4.33RR18 pKa = 11.84SCCIKK23 pKa = 10.13TSLTATSEE31 pKa = 4.18ATSWAIPPIAICCCCCLCCAGGLYY55 pKa = 10.17LVHH58 pKa = 6.86SGRR61 pKa = 11.84LPSISRR67 pKa = 11.84RR68 pKa = 11.84LDD70 pKa = 3.01VLRR73 pKa = 11.84DD74 pKa = 3.51SRR76 pKa = 11.84STPEE80 pKa = 3.57HH81 pKa = 6.1KK82 pKa = 10.3VRR84 pKa = 11.84RR85 pKa = 11.84DD86 pKa = 3.06RR87 pKa = 11.84FTKK90 pKa = 10.09PRR92 pKa = 11.84IHH94 pKa = 7.26RR95 pKa = 11.84PRR97 pKa = 11.84LSSTSDD103 pKa = 3.15SSDD106 pKa = 3.32LTDD109 pKa = 6.21LEE111 pKa = 4.68LSWDD115 pKa = 3.79GFDD118 pKa = 4.21PLAYY122 pKa = 9.1PHH124 pKa = 6.97WSAVNSPRR132 pKa = 11.84ARR134 pKa = 11.84PAPSVHH140 pKa = 5.86SQGLIPLVTLQPRR153 pKa = 11.84AGLDD157 pKa = 3.05NGVVHH162 pKa = 6.37SQPSRR167 pKa = 11.84SPRR170 pKa = 11.84PHH172 pKa = 5.93QRR174 pKa = 11.84FEE176 pKa = 4.34DD177 pKa = 3.76WLQQAHH183 pKa = 7.19LLRR186 pKa = 11.84PGDD189 pKa = 3.72VPRR192 pKa = 11.84DD193 pKa = 3.7TNPFRR198 pKa = 11.84RR199 pKa = 11.84PYY201 pKa = 9.57PRR203 pKa = 11.84RR204 pKa = 11.84DD205 pKa = 3.35TEE207 pKa = 4.01HH208 pKa = 6.66

Molecular weight: 23.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7760

208

1297

646.7

70.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.013 ± 0.378

1.198 ± 0.187

5.528 ± 0.182

3.119 ± 0.357

3.363 ± 0.209

5.58 ± 0.311

2.255 ± 0.218

4.936 ± 0.29

2.255 ± 0.327

10.18 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.236

3.956 ± 0.326

8.222 ± 0.394

3.814 ± 0.268

5.799 ± 0.246

7.887 ± 0.519

7.384 ± 0.289

7.655 ± 0.361

1.559 ± 0.2

2.912 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski