Ferruginivarius sediminum
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3868 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369T671|A0A369T671_9PROT DUF1491 family protein OS=Ferruginivarius sediminum OX=2661937 GN=DRB17_15935 PE=4 SV=1
MM1 pKa = 7.84 AEE3 pKa = 3.92 QPGTGGLAEE12 pKa = 4.14 TDD14 pKa = 3.44 EE15 pKa = 4.68 AALDD19 pKa = 3.48 IDD21 pKa = 4.14 VTKK24 pKa = 10.71 VCYY27 pKa = 9.84 IVVKK31 pKa = 10.64 ARR33 pKa = 11.84 EE34 pKa = 3.73 LDD36 pKa = 3.36 ARR38 pKa = 11.84 EE39 pKa = 4.28 DD40 pKa = 3.85 VVEE43 pKa = 4.57 PDD45 pKa = 3.66 YY46 pKa = 11.65 ASNAADD52 pKa = 3.66 DD53 pKa = 3.57 QCRR56 pKa = 11.84 QVLEE60 pKa = 5.14 AYY62 pKa = 10.42 ADD64 pKa = 3.71 DD65 pKa = 4.28 PVFDD69 pKa = 4.61 EE70 pKa = 5.08 LKK72 pKa = 10.67 QAIDD76 pKa = 3.64 DD77 pKa = 4.59 LNWDD81 pKa = 3.81 EE82 pKa = 4.21 QCTVVALAWVGRR94 pKa = 11.84 GTYY97 pKa = 10.59 AKK99 pKa = 10.39 GDD101 pKa = 3.46 WAEE104 pKa = 4.19 ALQAAQDD111 pKa = 3.66 EE112 pKa = 4.96 HH113 pKa = 6.58 TDD115 pKa = 3.44 NTSLYY120 pKa = 9.74 LLGMPLLADD129 pKa = 3.68 YY130 pKa = 11.16 LEE132 pKa = 4.82 EE133 pKa = 4.77 GLAAFDD139 pKa = 4.73 LSCEE143 pKa = 4.06 DD144 pKa = 4.44 FEE146 pKa = 4.94 MGHH149 pKa = 6.74 LL150 pKa = 3.8
Molecular weight: 16.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.681
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 4.012
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.795
Patrickios 1.036
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A369T7C9|A0A369T7C9_9PROT MerR family transcriptional regulator OS=Ferruginivarius sediminum OX=2661937 GN=DRB17_16420 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLVRR12 pKa = 11.84 KK13 pKa = 9.31 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.43 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.96 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3868
0
3868
1237093
28
9591
319.8
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.306 ± 0.063
0.924 ± 0.015
6.02 ± 0.048
6.52 ± 0.042
3.429 ± 0.03
8.911 ± 0.048
2.149 ± 0.019
4.477 ± 0.03
2.869 ± 0.036
10.076 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.025
2.476 ± 0.025
5.155 ± 0.03
3.158 ± 0.021
7.865 ± 0.053
4.878 ± 0.032
5.058 ± 0.032
7.7 ± 0.03
1.336 ± 0.016
2.296 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here