Streptococcus phage phiBHN167

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4KJ98|U4KJ98_9CAUD Phage GP6 protein OS=Streptococcus phage phiBHN167 OX=1267169 GN=phiBHN167_00160 PE=4 SV=1
MM1 pKa = 7.68ALYY4 pKa = 10.35KK5 pKa = 9.71ATKK8 pKa = 10.2NLFFEE13 pKa = 4.51QLNMDD18 pKa = 4.57VIVNDD23 pKa = 4.33IIEE26 pKa = 4.43LDD28 pKa = 3.17EE29 pKa = 5.98DD30 pKa = 3.97YY31 pKa = 11.46AKK33 pKa = 10.68EE34 pKa = 4.11VNKK37 pKa = 10.32KK38 pKa = 10.01LKK40 pKa = 10.48NAFPDD45 pKa = 3.51VKK47 pKa = 10.82NVLEE51 pKa = 4.45LVDD54 pKa = 4.28KK55 pKa = 11.37NGTLEE60 pKa = 4.47PEE62 pKa = 4.5DD63 pKa = 4.44APSVDD68 pKa = 4.54DD69 pKa = 4.69ASQATVEE76 pKa = 4.33DD77 pKa = 3.91

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4KJ87|U4KJ87_9CAUD Phage GP20 protein OS=Streptococcus phage phiBHN167 OX=1267169 GN=phiBHN167_00130 PE=4 SV=1
MM1 pKa = 7.5NIAIKK6 pKa = 10.66VDD8 pKa = 3.66LQKK11 pKa = 11.21AKK13 pKa = 10.59QKK15 pKa = 10.71LSNEE19 pKa = 3.7SMTRR23 pKa = 11.84GKK25 pKa = 10.05IAVASKK31 pKa = 10.45ILLDD35 pKa = 3.62NEE37 pKa = 4.16QYY39 pKa = 10.4IPLRR43 pKa = 11.84GGEE46 pKa = 4.01LRR48 pKa = 11.84ASGRR52 pKa = 11.84IVGQGDD58 pKa = 3.52AVVYY62 pKa = 7.1GTVYY66 pKa = 10.93ARR68 pKa = 11.84AQFYY72 pKa = 9.69GANGIVTFRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 5.87TTPGTGKK90 pKa = 10.24RR91 pKa = 11.84WDD93 pKa = 3.44QVATSKK99 pKa = 10.7HH100 pKa = 5.05AEE102 pKa = 3.36EE103 pKa = 4.01WARR106 pKa = 11.84AFVKK110 pKa = 10.83GMGLL114 pKa = 3.32

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10347

40

1093

211.2

23.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.142 ± 0.597

0.435 ± 0.075

6.514 ± 0.275

7.548 ± 0.502

4.252 ± 0.277

6.359 ± 0.422

1.508 ± 0.198

6.707 ± 0.301

8.592 ± 0.426

7.654 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.172

6.06 ± 0.309

2.735 ± 0.229

4.465 ± 0.287

4.127 ± 0.252

5.886 ± 0.482

6.214 ± 0.349

6.166 ± 0.31

1.285 ± 0.198

3.885 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski