Amino acid dipepetide frequency for Streptococcus phage phiBHN167

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.543AlaAla: 4.543 ± 1.171
0.483AlaCys: 0.483 ± 0.202
4.639AlaAsp: 4.639 ± 0.727
6.573AlaGlu: 6.573 ± 0.837
2.9AlaPhe: 2.9 ± 0.482
3.866AlaGly: 3.866 ± 0.675
0.773AlaHis: 0.773 ± 0.231
5.509AlaIle: 5.509 ± 0.662
5.606AlaLys: 5.606 ± 0.831
6.379AlaLeu: 6.379 ± 1.114
2.223AlaMet: 2.223 ± 0.616
3.866AlaAsn: 3.866 ± 0.678
1.45AlaPro: 1.45 ± 0.352
2.706AlaGln: 2.706 ± 0.673
3.093AlaArg: 3.093 ± 0.689
4.35AlaSer: 4.35 ± 0.83
4.833AlaThr: 4.833 ± 0.796
4.833AlaVal: 4.833 ± 0.761
0.58AlaTrp: 0.58 ± 0.252
2.223AlaTyr: 2.223 ± 0.539
0.0AlaXaa: 0.0 ± 0.0
Cys
0.387CysAla: 0.387 ± 0.203
0.097CysCys: 0.097 ± 0.088
0.193CysAsp: 0.193 ± 0.136
0.58CysGlu: 0.58 ± 0.217
0.29CysPhe: 0.29 ± 0.16
0.29CysGly: 0.29 ± 0.16
0.0CysHis: 0.0 ± 0.0
0.097CysIle: 0.097 ± 0.1
0.387CysLys: 0.387 ± 0.196
0.29CysLeu: 0.29 ± 0.139
0.097CysMet: 0.097 ± 0.083
0.0CysAsn: 0.0 ± 0.0
0.387CysPro: 0.387 ± 0.181
0.193CysGln: 0.193 ± 0.125
0.0CysArg: 0.0 ± 0.0
0.193CysSer: 0.193 ± 0.136
0.097CysThr: 0.097 ± 0.106
0.58CysVal: 0.58 ± 0.21
0.193CysTrp: 0.193 ± 0.154
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.06AspAla: 4.06 ± 0.636
0.29AspCys: 0.29 ± 0.166
4.06AspAsp: 4.06 ± 0.844
4.06AspGlu: 4.06 ± 0.568
4.446AspPhe: 4.446 ± 0.693
6.283AspGly: 6.283 ± 0.835
0.58AspHis: 0.58 ± 0.185
5.123AspIle: 5.123 ± 0.607
6.283AspLys: 6.283 ± 0.665
4.543AspLeu: 4.543 ± 0.778
1.546AspMet: 1.546 ± 0.305
3.48AspAsn: 3.48 ± 0.542
1.353AspPro: 1.353 ± 0.425
1.45AspGln: 1.45 ± 0.349
2.803AspArg: 2.803 ± 0.507
2.61AspSer: 2.61 ± 0.624
3.576AspThr: 3.576 ± 0.491
4.06AspVal: 4.06 ± 0.637
0.483AspTrp: 0.483 ± 0.27
4.06AspTyr: 4.06 ± 0.65
0.0AspXaa: 0.0 ± 0.0
Glu
4.253GluAla: 4.253 ± 0.796
0.097GluCys: 0.097 ± 0.1
4.35GluAsp: 4.35 ± 0.799
6.863GluGlu: 6.863 ± 1.052
2.9GluPhe: 2.9 ± 0.567
2.513GluGly: 2.513 ± 0.492
0.967GluHis: 0.967 ± 0.266
6.283GluIle: 6.283 ± 0.865
6.476GluLys: 6.476 ± 0.82
8.409GluLeu: 8.409 ± 0.955
2.706GluMet: 2.706 ± 0.541
4.156GluAsn: 4.156 ± 0.673
1.74GluPro: 1.74 ± 0.516
5.026GluGln: 5.026 ± 0.662
4.06GluArg: 4.06 ± 0.809
2.9GluSer: 2.9 ± 0.635
3.673GluThr: 3.673 ± 0.586
4.543GluVal: 4.543 ± 0.74
0.387GluTrp: 0.387 ± 0.183
3.19GluTyr: 3.19 ± 0.622
0.0GluXaa: 0.0 ± 0.0
Phe
2.416PheAla: 2.416 ± 0.53
0.097PheCys: 0.097 ± 0.097
4.929PheAsp: 4.929 ± 0.604
4.833PheGlu: 4.833 ± 0.885
1.643PhePhe: 1.643 ± 0.48
2.223PheGly: 2.223 ± 0.462
0.677PheHis: 0.677 ± 0.286
2.513PheIle: 2.513 ± 0.451
2.513PheLys: 2.513 ± 0.559
1.933PheLeu: 1.933 ± 0.354
1.257PheMet: 1.257 ± 0.348
2.803PheAsn: 2.803 ± 0.414
0.87PhePro: 0.87 ± 0.234
2.223PheGln: 2.223 ± 0.576
1.546PheArg: 1.546 ± 0.404
3.093PheSer: 3.093 ± 0.44
3.286PheThr: 3.286 ± 0.698
1.933PheVal: 1.933 ± 0.41
0.193PheTrp: 0.193 ± 0.139
1.546PheTyr: 1.546 ± 0.467
0.0PheXaa: 0.0 ± 0.0
Gly
4.446GlyAla: 4.446 ± 0.909
0.29GlyCys: 0.29 ± 0.166
2.61GlyAsp: 2.61 ± 0.505
3.286GlyGlu: 3.286 ± 0.466
3.19GlyPhe: 3.19 ± 0.739
5.123GlyGly: 5.123 ± 0.803
1.257GlyHis: 1.257 ± 0.315
4.35GlyIle: 4.35 ± 0.609
4.639GlyLys: 4.639 ± 0.686
4.639GlyLeu: 4.639 ± 0.889
2.223GlyMet: 2.223 ± 0.506
4.06GlyAsn: 4.06 ± 0.773
0.29GlyPro: 0.29 ± 0.17
2.416GlyGln: 2.416 ± 0.548
3.093GlyArg: 3.093 ± 0.416
4.833GlySer: 4.833 ± 1.065
4.156GlyThr: 4.156 ± 0.74
5.316GlyVal: 5.316 ± 0.844
1.257GlyTrp: 1.257 ± 0.536
2.126GlyTyr: 2.126 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
1.063HisAla: 1.063 ± 0.331
0.193HisCys: 0.193 ± 0.14
0.773HisAsp: 0.773 ± 0.297
0.87HisGlu: 0.87 ± 0.306
0.58HisPhe: 0.58 ± 0.22
1.063HisGly: 1.063 ± 0.391
0.29HisHis: 0.29 ± 0.17
1.257HisIle: 1.257 ± 0.429
1.16HisLys: 1.16 ± 0.337
0.87HisLeu: 0.87 ± 0.331
0.29HisMet: 0.29 ± 0.164
0.58HisAsn: 0.58 ± 0.24
0.773HisPro: 0.773 ± 0.302
0.29HisGln: 0.29 ± 0.143
0.483HisArg: 0.483 ± 0.212
1.74HisSer: 1.74 ± 0.545
1.16HisThr: 1.16 ± 0.359
0.677HisVal: 0.677 ± 0.34
0.29HisTrp: 0.29 ± 0.176
0.677HisTyr: 0.677 ± 0.321
0.0HisXaa: 0.0 ± 0.0
Ile
5.219IleAla: 5.219 ± 0.702
0.29IleCys: 0.29 ± 0.177
4.639IleAsp: 4.639 ± 0.654
7.346IleGlu: 7.346 ± 1.033
1.836IlePhe: 1.836 ± 0.404
4.06IleGly: 4.06 ± 0.551
0.967IleHis: 0.967 ± 0.276
4.35IleIle: 4.35 ± 0.818
7.732IleLys: 7.732 ± 0.995
3.77IleLeu: 3.77 ± 0.681
1.643IleMet: 1.643 ± 0.381
4.06IleAsn: 4.06 ± 0.454
2.32IlePro: 2.32 ± 0.575
2.416IleGln: 2.416 ± 0.369
2.03IleArg: 2.03 ± 0.481
4.35IleSer: 4.35 ± 0.53
3.866IleThr: 3.866 ± 0.672
3.77IleVal: 3.77 ± 0.549
0.677IleTrp: 0.677 ± 0.233
1.74IleTyr: 1.74 ± 0.457
0.0IleXaa: 0.0 ± 0.0
Lys
6.283LysAla: 6.283 ± 1.146
0.387LysCys: 0.387 ± 0.202
4.156LysAsp: 4.156 ± 0.758
6.476LysGlu: 6.476 ± 0.923
2.803LysPhe: 2.803 ± 0.555
5.703LysGly: 5.703 ± 0.954
1.546LysHis: 1.546 ± 0.438
5.509LysIle: 5.509 ± 0.799
7.153LysLys: 7.153 ± 1.131
7.442LysLeu: 7.442 ± 1.175
1.643LysMet: 1.643 ± 0.348
6.089LysAsn: 6.089 ± 0.824
2.126LysPro: 2.126 ± 0.501
3.866LysGln: 3.866 ± 0.69
3.48LysArg: 3.48 ± 0.547
5.026LysSer: 5.026 ± 0.683
5.799LysThr: 5.799 ± 0.77
5.703LysVal: 5.703 ± 0.704
1.45LysTrp: 1.45 ± 0.363
3.286LysTyr: 3.286 ± 0.567
0.0LysXaa: 0.0 ± 0.0
Leu
6.573LeuAla: 6.573 ± 0.909
0.29LeuCys: 0.29 ± 0.168
5.703LeuAsp: 5.703 ± 0.677
6.283LeuGlu: 6.283 ± 0.96
2.996LeuPhe: 2.996 ± 0.697
4.446LeuGly: 4.446 ± 0.661
1.257LeuHis: 1.257 ± 0.386
2.803LeuIle: 2.803 ± 0.679
8.216LeuLys: 8.216 ± 0.965
4.929LeuLeu: 4.929 ± 0.71
1.353LeuMet: 1.353 ± 0.33
5.509LeuAsn: 5.509 ± 0.865
3.286LeuPro: 3.286 ± 0.55
3.77LeuGln: 3.77 ± 0.87
2.9LeuArg: 2.9 ± 0.621
4.253LeuSer: 4.253 ± 0.644
5.316LeuThr: 5.316 ± 0.811
3.963LeuVal: 3.963 ± 0.888
0.677LeuTrp: 0.677 ± 0.226
2.03LeuTyr: 2.03 ± 0.301
0.0LeuXaa: 0.0 ± 0.0
Met
2.61MetAla: 2.61 ± 0.582
0.193MetCys: 0.193 ± 0.137
1.353MetAsp: 1.353 ± 0.289
1.546MetGlu: 1.546 ± 0.354
0.677MetPhe: 0.677 ± 0.256
1.063MetGly: 1.063 ± 0.28
0.097MetHis: 0.097 ± 0.104
1.45MetIle: 1.45 ± 0.45
2.03MetLys: 2.03 ± 0.428
1.257MetLeu: 1.257 ± 0.287
0.58MetMet: 0.58 ± 0.168
1.74MetAsn: 1.74 ± 0.401
0.773MetPro: 0.773 ± 0.252
1.45MetGln: 1.45 ± 0.358
2.03MetArg: 2.03 ± 0.403
1.643MetSer: 1.643 ± 0.41
2.126MetThr: 2.126 ± 0.362
1.16MetVal: 1.16 ± 0.287
0.29MetTrp: 0.29 ± 0.164
0.58MetTyr: 0.58 ± 0.24
0.0MetXaa: 0.0 ± 0.0
Asn
4.929AsnAla: 4.929 ± 0.616
0.29AsnCys: 0.29 ± 0.224
4.639AsnAsp: 4.639 ± 0.645
3.77AsnGlu: 3.77 ± 0.604
2.513AsnPhe: 2.513 ± 0.668
5.026AsnGly: 5.026 ± 0.914
1.063AsnHis: 1.063 ± 0.31
3.866AsnIle: 3.866 ± 0.626
4.833AsnLys: 4.833 ± 0.581
4.929AsnLeu: 4.929 ± 1.011
1.74AsnMet: 1.74 ± 0.426
4.156AsnAsn: 4.156 ± 0.768
2.03AsnPro: 2.03 ± 0.567
2.706AsnGln: 2.706 ± 0.487
2.03AsnArg: 2.03 ± 0.526
3.576AsnSer: 3.576 ± 0.569
2.9AsnThr: 2.9 ± 0.467
2.61AsnVal: 2.61 ± 0.573
0.967AsnTrp: 0.967 ± 0.313
2.03AsnTyr: 2.03 ± 0.613
0.0AsnXaa: 0.0 ± 0.0
Pro
1.546ProAla: 1.546 ± 0.364
0.193ProCys: 0.193 ± 0.141
2.03ProAsp: 2.03 ± 0.504
2.223ProGlu: 2.223 ± 0.366
1.45ProPhe: 1.45 ± 0.448
0.87ProGly: 0.87 ± 0.307
0.387ProHis: 0.387 ± 0.205
2.03ProIle: 2.03 ± 0.425
2.416ProLys: 2.416 ± 0.675
1.74ProLeu: 1.74 ± 0.467
0.29ProMet: 0.29 ± 0.198
1.933ProAsn: 1.933 ± 0.564
0.773ProPro: 0.773 ± 0.302
1.45ProGln: 1.45 ± 0.373
0.967ProArg: 0.967 ± 0.286
1.933ProSer: 1.933 ± 0.404
1.74ProThr: 1.74 ± 0.428
1.74ProVal: 1.74 ± 0.444
0.29ProTrp: 0.29 ± 0.197
1.353ProTyr: 1.353 ± 0.418
0.0ProXaa: 0.0 ± 0.0
Gln
3.48GlnAla: 3.48 ± 0.629
0.193GlnCys: 0.193 ± 0.156
1.74GlnAsp: 1.74 ± 0.414
3.383GlnGlu: 3.383 ± 0.626
1.74GlnPhe: 1.74 ± 0.45
2.803GlnGly: 2.803 ± 0.839
0.773GlnHis: 0.773 ± 0.287
2.803GlnIle: 2.803 ± 0.431
5.123GlnLys: 5.123 ± 0.858
2.996GlnLeu: 2.996 ± 0.468
1.257GlnMet: 1.257 ± 0.36
2.416GlnAsn: 2.416 ± 0.425
1.063GlnPro: 1.063 ± 0.277
2.9GlnGln: 2.9 ± 0.851
2.126GlnArg: 2.126 ± 0.465
2.32GlnSer: 2.32 ± 0.57
1.836GlnThr: 1.836 ± 0.545
3.286GlnVal: 3.286 ± 0.592
0.483GlnTrp: 0.483 ± 0.204
1.933GlnTyr: 1.933 ± 0.445
0.0GlnXaa: 0.0 ± 0.0
Arg
3.576ArgAla: 3.576 ± 0.642
0.097ArgCys: 0.097 ± 0.117
2.32ArgAsp: 2.32 ± 0.461
2.61ArgGlu: 2.61 ± 0.653
1.836ArgPhe: 1.836 ± 0.358
2.513ArgGly: 2.513 ± 0.469
0.677ArgHis: 0.677 ± 0.284
2.61ArgIle: 2.61 ± 0.554
3.286ArgLys: 3.286 ± 0.645
4.06ArgLeu: 4.06 ± 0.679
0.967ArgMet: 0.967 ± 0.266
2.61ArgAsn: 2.61 ± 0.545
1.546ArgPro: 1.546 ± 0.504
1.933ArgGln: 1.933 ± 0.299
1.643ArgArg: 1.643 ± 0.358
1.546ArgSer: 1.546 ± 0.344
2.223ArgThr: 2.223 ± 0.462
2.513ArgVal: 2.513 ± 0.432
0.58ArgTrp: 0.58 ± 0.265
2.03ArgTyr: 2.03 ± 0.456
0.0ArgXaa: 0.0 ± 0.0
Ser
4.35SerAla: 4.35 ± 0.681
0.193SerCys: 0.193 ± 0.131
4.35SerAsp: 4.35 ± 0.753
4.35SerGlu: 4.35 ± 0.963
2.416SerPhe: 2.416 ± 0.586
3.866SerGly: 3.866 ± 1.09
1.45SerHis: 1.45 ± 0.498
2.803SerIle: 2.803 ± 0.476
3.963SerLys: 3.963 ± 0.754
4.639SerLeu: 4.639 ± 0.666
1.933SerMet: 1.933 ± 0.394
3.19SerAsn: 3.19 ± 0.568
1.836SerPro: 1.836 ± 0.388
2.61SerGln: 2.61 ± 0.468
2.223SerArg: 2.223 ± 0.397
3.963SerSer: 3.963 ± 0.93
3.673SerThr: 3.673 ± 0.496
4.156SerVal: 4.156 ± 0.68
1.063SerTrp: 1.063 ± 0.27
1.836SerTyr: 1.836 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
3.673ThrAla: 3.673 ± 1.137
0.387ThrCys: 0.387 ± 0.147
4.446ThrAsp: 4.446 ± 0.627
4.156ThrGlu: 4.156 ± 0.569
2.996ThrPhe: 2.996 ± 0.411
4.736ThrGly: 4.736 ± 0.803
0.483ThrHis: 0.483 ± 0.205
5.606ThrIle: 5.606 ± 0.683
5.026ThrLys: 5.026 ± 0.675
5.703ThrLeu: 5.703 ± 1.008
0.967ThrMet: 0.967 ± 0.283
4.156ThrAsn: 4.156 ± 0.566
2.223ThrPro: 2.223 ± 0.351
2.126ThrGln: 2.126 ± 0.474
2.126ThrArg: 2.126 ± 0.405
2.513ThrSer: 2.513 ± 0.344
3.673ThrThr: 3.673 ± 0.566
4.253ThrVal: 4.253 ± 0.699
1.063ThrTrp: 1.063 ± 0.301
1.836ThrTyr: 1.836 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
5.026ValAla: 5.026 ± 0.674
0.193ValCys: 0.193 ± 0.128
4.446ValAsp: 4.446 ± 0.697
3.673ValGlu: 3.673 ± 0.601
2.223ValPhe: 2.223 ± 0.453
4.156ValGly: 4.156 ± 0.645
1.16ValHis: 1.16 ± 0.373
5.219ValIle: 5.219 ± 0.658
5.219ValLys: 5.219 ± 0.64
3.093ValLeu: 3.093 ± 0.512
0.773ValMet: 0.773 ± 0.229
2.996ValAsn: 2.996 ± 0.566
0.773ValPro: 0.773 ± 0.246
2.706ValGln: 2.706 ± 0.491
1.836ValArg: 1.836 ± 0.385
4.543ValSer: 4.543 ± 0.651
5.509ValThr: 5.509 ± 0.662
4.35ValVal: 4.35 ± 0.758
1.16ValTrp: 1.16 ± 0.403
2.61ValTyr: 2.61 ± 0.548
0.0ValXaa: 0.0 ± 0.0
Trp
1.353TrpAla: 1.353 ± 0.315
0.0TrpCys: 0.0 ± 0.0
0.58TrpAsp: 0.58 ± 0.255
0.483TrpGlu: 0.483 ± 0.18
0.58TrpPhe: 0.58 ± 0.229
0.387TrpGly: 0.387 ± 0.174
0.387TrpHis: 0.387 ± 0.211
0.773TrpIle: 0.773 ± 0.289
1.353TrpLys: 1.353 ± 0.343
1.16TrpLeu: 1.16 ± 0.33
0.29TrpMet: 0.29 ± 0.176
0.483TrpAsn: 0.483 ± 0.17
0.193TrpPro: 0.193 ± 0.19
0.773TrpGln: 0.773 ± 0.253
0.387TrpArg: 0.387 ± 0.19
0.677TrpSer: 0.677 ± 0.184
0.58TrpThr: 0.58 ± 0.29
0.773TrpVal: 0.773 ± 0.218
0.29TrpTrp: 0.29 ± 0.164
1.353TrpTyr: 1.353 ± 0.672
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.643TyrAla: 1.643 ± 0.386
0.097TyrCys: 0.097 ± 0.106
3.19TyrAsp: 3.19 ± 0.545
2.126TyrGlu: 2.126 ± 0.473
2.513TyrPhe: 2.513 ± 0.562
2.61TyrGly: 2.61 ± 0.467
0.29TyrHis: 0.29 ± 0.147
2.126TyrIle: 2.126 ± 0.386
2.513TyrLys: 2.513 ± 0.546
3.77TyrLeu: 3.77 ± 0.748
0.773TyrMet: 0.773 ± 0.357
2.32TyrAsn: 2.32 ± 0.504
1.546TyrPro: 1.546 ± 0.41
1.643TyrGln: 1.643 ± 0.371
2.416TyrArg: 2.416 ± 0.52
2.803TyrSer: 2.803 ± 0.458
2.126TyrThr: 2.126 ± 0.51
1.45TyrVal: 1.45 ± 0.34
0.483TyrTrp: 0.483 ± 0.253
2.416TyrTyr: 2.416 ± 0.747
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (10347 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski