Corynebacterium phage PeteyPab
Average proteome isoelectric point is 5.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3MB87|A0A3G3MB87_9CAUD Uncharacterized protein OS=Corynebacterium phage PeteyPab OX=2483663 GN=69 PE=4 SV=1
MM1 pKa = 8.02 PDD3 pKa = 3.07 FDD5 pKa = 4.63 SFHH8 pKa = 6.6 MEE10 pKa = 3.73 NDD12 pKa = 3.53 VVDD15 pKa = 4.01 TSTNLAYY22 pKa = 10.55 LDD24 pKa = 3.75 YY25 pKa = 11.23 LNRR28 pKa = 4.36
Molecular weight: 3.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.859
IPC_protein 3.681
Toseland 3.49
ProMoST 3.935
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.757
Rodwell 3.541
Grimsley 3.427
Solomon 3.668
Lehninger 3.63
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.834
Patrickios 1.914
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A3G3MA78|A0A3G3MA78_9CAUD WhiB family transcription factor OS=Corynebacterium phage PeteyPab OX=2483663 GN=41 PE=4 SV=1
MM1 pKa = 7.61 GKK3 pKa = 9.83 LVWPNYY9 pKa = 9.37 GPPRR13 pKa = 11.84 YY14 pKa = 10.0 DD15 pKa = 3.92 IINKK19 pKa = 9.82 GYY21 pKa = 10.71 LKK23 pKa = 10.75 ARR25 pKa = 11.84 LEE27 pKa = 4.27 AQSSHH32 pKa = 5.11 KK33 pKa = 10.38 HH34 pKa = 5.15 PVYY37 pKa = 10.76 SSDD40 pKa = 2.46 WFSFQEE46 pKa = 4.22 SVLNNNPYY54 pKa = 9.84 VEE56 pKa = 4.11 RR57 pKa = 11.84 ALFKK61 pKa = 10.53 LAQKK65 pKa = 10.74 AVFALKK71 pKa = 10.46 RR72 pKa = 11.84 EE73 pKa = 4.05 IPIGDD78 pKa = 3.63 NSKK81 pKa = 10.96 DD82 pKa = 2.97 GHH84 pKa = 6.61 LRR86 pKa = 11.84 NKK88 pKa = 8.91 VKK90 pKa = 10.5 AYY92 pKa = 9.73 RR93 pKa = 11.84 VRR95 pKa = 11.84 NGGLAKK101 pKa = 10.52 DD102 pKa = 3.38 RR103 pKa = 11.84 TVYY106 pKa = 9.65 EE107 pKa = 4.13 VHH109 pKa = 6.98 AGGHH113 pKa = 4.57 TKK115 pKa = 9.9 EE116 pKa = 5.51 DD117 pKa = 3.33 RR118 pKa = 11.84 SKK120 pKa = 9.85 WHH122 pKa = 6.12 AALYY126 pKa = 10.1 RR127 pKa = 11.84 STYY130 pKa = 10.89 SSAEE134 pKa = 3.86 DD135 pKa = 3.45 YY136 pKa = 11.15 KK137 pKa = 11.22 NRR139 pKa = 11.84 KK140 pKa = 7.4 LTEE143 pKa = 3.97 TGDD146 pKa = 3.7 RR147 pKa = 11.84 IGWLRR152 pKa = 11.84 SAEE155 pKa = 3.95 RR156 pKa = 11.84 RR157 pKa = 11.84 LSDD160 pKa = 3.23 GG161 pKa = 3.62
Molecular weight: 18.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.151
IPC2_protein 9.414
IPC_protein 9.472
Toseland 9.984
ProMoST 9.706
Dawson 10.218
Bjellqvist 9.882
Wikipedia 10.394
Rodwell 10.599
Grimsley 10.292
Solomon 10.233
Lehninger 10.204
Nozaki 9.955
DTASelect 9.882
Thurlkill 10.058
EMBOSS 10.394
Sillero 10.116
Patrickios 10.043
IPC_peptide 10.233
IPC2_peptide 8.434
IPC2.peptide.svr19 8.451
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
91
0
91
19975
23
2143
219.5
24.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.856 ± 0.446
0.626 ± 0.093
6.909 ± 0.22
7.94 ± 0.324
3.835 ± 0.191
7.384 ± 0.421
1.697 ± 0.153
5.817 ± 0.146
5.126 ± 0.183
7.845 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.603 ± 0.15
4.245 ± 0.209
4.375 ± 0.165
3.094 ± 0.13
5.902 ± 0.234
6.418 ± 0.198
5.537 ± 0.262
6.763 ± 0.213
1.632 ± 0.107
3.394 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here