Riemerella phage RAP44

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5A3C5|F5A3C5_9CAUD Phage head morphogenesis protein OS=Riemerella phage RAP44 OX=936152 PE=4 SV=1
MM1 pKa = 7.67GNLEE5 pKa = 3.83KK6 pKa = 10.82SKK8 pKa = 10.89NINEE12 pKa = 4.58IIEE15 pKa = 4.37STSFRR20 pKa = 11.84EE21 pKa = 4.29EE22 pKa = 3.35IEE24 pKa = 4.02EE25 pKa = 4.54AIEE28 pKa = 4.13SGMFTDD34 pKa = 4.04YY35 pKa = 11.17AYY37 pKa = 10.52DD38 pKa = 4.18GEE40 pKa = 4.81DD41 pKa = 3.91EE42 pKa = 4.61YY43 pKa = 10.95PVDD46 pKa = 3.79EE47 pKa = 4.96FDD49 pKa = 6.38SIFALDD55 pKa = 3.82AVIKK59 pKa = 9.99VIKK62 pKa = 10.26KK63 pKa = 7.31YY64 pKa = 10.81FCTEE68 pKa = 3.75EE69 pKa = 3.95NTNTT73 pKa = 3.65

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5A3G3|F5A3G3_9CAUD Uncharacterized protein OS=Riemerella phage RAP44 OX=936152 PE=4 SV=1
MM1 pKa = 7.23TAEE4 pKa = 3.66QLIPIFRR11 pKa = 11.84TQLTLVCDD19 pKa = 4.02PKK21 pKa = 10.36EE22 pKa = 4.15TEE24 pKa = 4.5KK25 pKa = 10.35IHH27 pKa = 6.82RR28 pKa = 11.84WMLGVAEE35 pKa = 4.61SKK37 pKa = 10.5EE38 pKa = 4.13SRR40 pKa = 11.84KK41 pKa = 9.92KK42 pKa = 10.25RR43 pKa = 11.84LHH45 pKa = 6.83AMFDD49 pKa = 3.14SKK51 pKa = 10.95EE52 pKa = 3.97RR53 pKa = 11.84YY54 pKa = 9.18KK55 pKa = 10.41IYY57 pKa = 9.9PKK59 pKa = 10.15PKK61 pKa = 8.45RR62 pKa = 11.84KK63 pKa = 9.32RR64 pKa = 11.84RR65 pKa = 11.84KK66 pKa = 9.12KK67 pKa = 10.58ALL69 pKa = 3.48

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14291

22

1393

178.6

20.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.668 ± 0.429

0.742 ± 0.113

5.521 ± 0.199

8.747 ± 0.348

4.744 ± 0.225

5.36 ± 0.366

1.392 ± 0.143

7.578 ± 0.231

10.3 ± 0.368

8.817 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.136

6.55 ± 0.26

2.666 ± 0.221

4.254 ± 0.284

4.128 ± 0.152

5.437 ± 0.239

5.528 ± 0.263

5.045 ± 0.227

1.106 ± 0.106

4.017 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski