Amino acid dipepetide frequency for Riemerella phage RAP44

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.26AlaAla: 1.26 ± 0.526
0.07AlaCys: 0.07 ± 0.065
3.569AlaAsp: 3.569 ± 0.418
5.528AlaGlu: 5.528 ± 0.673
2.099AlaPhe: 2.099 ± 0.592
3.079AlaGly: 3.079 ± 0.759
0.84AlaHis: 0.84 ± 0.221
4.829AlaIle: 4.829 ± 0.509
6.858AlaLys: 6.858 ± 1.053
5.668AlaLeu: 5.668 ± 0.668
1.4AlaMet: 1.4 ± 0.378
3.779AlaAsn: 3.779 ± 0.49
1.26AlaPro: 1.26 ± 0.262
2.659AlaGln: 2.659 ± 0.592
2.379AlaArg: 2.379 ± 0.366
2.099AlaSer: 2.099 ± 0.355
3.359AlaThr: 3.359 ± 0.724
2.799AlaVal: 2.799 ± 0.524
0.7AlaTrp: 0.7 ± 0.293
2.449AlaTyr: 2.449 ± 0.488
0.0AlaXaa: 0.0 ± 0.0
Cys
0.21CysAla: 0.21 ± 0.131
0.0CysCys: 0.0 ± 0.0
0.28CysAsp: 0.28 ± 0.127
0.77CysGlu: 0.77 ± 0.227
0.42CysPhe: 0.42 ± 0.169
0.63CysGly: 0.63 ± 0.221
0.28CysHis: 0.28 ± 0.147
0.21CysIle: 0.21 ± 0.176
0.42CysLys: 0.42 ± 0.155
0.77CysLeu: 0.77 ± 0.275
0.07CysMet: 0.07 ± 0.069
0.42CysAsn: 0.42 ± 0.22
0.28CysPro: 0.28 ± 0.141
0.07CysGln: 0.07 ± 0.056
0.56CysArg: 0.56 ± 0.23
0.42CysSer: 0.42 ± 0.173
0.49CysThr: 0.49 ± 0.174
0.49CysVal: 0.49 ± 0.187
0.21CysTrp: 0.21 ± 0.124
0.42CysTyr: 0.42 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
2.939AspAla: 2.939 ± 0.576
0.35AspCys: 0.35 ± 0.142
3.429AspAsp: 3.429 ± 0.514
5.388AspGlu: 5.388 ± 0.63
4.059AspPhe: 4.059 ± 0.623
2.729AspGly: 2.729 ± 0.451
0.49AspHis: 0.49 ± 0.186
4.339AspIle: 4.339 ± 0.583
4.549AspLys: 4.549 ± 0.694
6.088AspLeu: 6.088 ± 0.701
1.26AspMet: 1.26 ± 0.308
2.729AspAsn: 2.729 ± 0.467
0.91AspPro: 0.91 ± 0.243
0.56AspGln: 0.56 ± 0.18
2.379AspArg: 2.379 ± 0.519
2.379AspSer: 2.379 ± 0.359
2.729AspThr: 2.729 ± 0.363
3.639AspVal: 3.639 ± 0.4
1.12AspTrp: 1.12 ± 0.252
3.149AspTyr: 3.149 ± 0.443
0.0AspXaa: 0.0 ± 0.0
Glu
5.808GluAla: 5.808 ± 0.68
0.7GluCys: 0.7 ± 0.264
4.829GluAsp: 4.829 ± 0.543
7.138GluGlu: 7.138 ± 1.103
3.919GluPhe: 3.919 ± 0.487
3.989GluGly: 3.989 ± 0.547
0.63GluHis: 0.63 ± 0.222
8.607GluIle: 8.607 ± 0.73
9.097GluLys: 9.097 ± 1.005
8.467GluLeu: 8.467 ± 0.82
2.379GluMet: 2.379 ± 0.42
5.038GluAsn: 5.038 ± 0.578
1.749GluPro: 1.749 ± 0.336
4.199GluGln: 4.199 ± 0.563
4.409GluArg: 4.409 ± 0.555
3.289GluSer: 3.289 ± 0.497
3.639GluThr: 3.639 ± 0.461
5.318GluVal: 5.318 ± 0.76
0.84GluTrp: 0.84 ± 0.22
3.429GluTyr: 3.429 ± 0.52
0.0GluXaa: 0.0 ± 0.0
Phe
2.379PheAla: 2.379 ± 0.467
0.49PheCys: 0.49 ± 0.218
2.659PheAsp: 2.659 ± 0.395
4.129PheGlu: 4.129 ± 0.55
2.449PhePhe: 2.449 ± 0.454
3.009PheGly: 3.009 ± 0.456
0.7PheHis: 0.7 ± 0.198
3.429PheIle: 3.429 ± 0.553
3.919PheLys: 3.919 ± 0.506
3.499PheLeu: 3.499 ± 0.663
1.19PheMet: 1.19 ± 0.238
3.499PheAsn: 3.499 ± 0.466
1.54PhePro: 1.54 ± 0.284
1.61PheGln: 1.61 ± 0.388
2.379PheArg: 2.379 ± 0.363
2.869PheSer: 2.869 ± 0.468
2.729PheThr: 2.729 ± 0.436
2.869PheVal: 2.869 ± 0.457
0.42PheTrp: 0.42 ± 0.149
1.679PheTyr: 1.679 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
2.449GlyAla: 2.449 ± 0.366
0.28GlyCys: 0.28 ± 0.128
1.959GlyAsp: 1.959 ± 0.372
3.989GlyGlu: 3.989 ± 0.501
2.799GlyPhe: 2.799 ± 0.467
3.219GlyGly: 3.219 ± 0.611
0.84GlyHis: 0.84 ± 0.241
4.059GlyIle: 4.059 ± 0.617
5.388GlyLys: 5.388 ± 0.611
3.569GlyLeu: 3.569 ± 0.67
1.54GlyMet: 1.54 ± 0.385
4.829GlyAsn: 4.829 ± 0.563
0.21GlyPro: 0.21 ± 0.111
2.659GlyGln: 2.659 ± 0.523
2.379GlyArg: 2.379 ± 0.409
3.149GlySer: 3.149 ± 0.536
2.939GlyThr: 2.939 ± 0.425
3.289GlyVal: 3.289 ± 0.634
1.19GlyTrp: 1.19 ± 0.321
2.869GlyTyr: 2.869 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
0.63HisAla: 0.63 ± 0.187
0.21HisCys: 0.21 ± 0.123
0.28HisAsp: 0.28 ± 0.133
0.7HisGlu: 0.7 ± 0.264
0.7HisPhe: 0.7 ± 0.224
0.7HisGly: 0.7 ± 0.215
0.28HisHis: 0.28 ± 0.12
1.12HisIle: 1.12 ± 0.257
1.749HisLys: 1.749 ± 0.406
1.33HisLeu: 1.33 ± 0.382
0.07HisMet: 0.07 ± 0.08
0.84HisAsn: 0.84 ± 0.217
0.28HisPro: 0.28 ± 0.168
0.98HisGln: 0.98 ± 0.215
0.7HisArg: 0.7 ± 0.25
1.26HisSer: 1.26 ± 0.28
0.77HisThr: 0.77 ± 0.258
0.35HisVal: 0.35 ± 0.157
0.21HisTrp: 0.21 ± 0.124
0.77HisTyr: 0.77 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.129IleAla: 4.129 ± 0.481
0.56IleCys: 0.56 ± 0.181
5.038IleAsp: 5.038 ± 0.606
6.788IleGlu: 6.788 ± 0.704
3.009IlePhe: 3.009 ± 0.401
3.989IleGly: 3.989 ± 0.575
1.26IleHis: 1.26 ± 0.304
4.129IleIle: 4.129 ± 0.656
8.887IleLys: 8.887 ± 0.693
6.578IleLeu: 6.578 ± 0.918
1.749IleMet: 1.749 ± 0.329
4.549IleAsn: 4.549 ± 0.567
2.449IlePro: 2.449 ± 0.455
2.799IleGln: 2.799 ± 0.408
2.799IleArg: 2.799 ± 0.462
5.178IleSer: 5.178 ± 0.516
6.088IleThr: 6.088 ± 0.899
3.219IleVal: 3.219 ± 0.459
0.56IleTrp: 0.56 ± 0.261
2.029IleTyr: 2.029 ± 0.462
0.0IleXaa: 0.0 ± 0.0
Lys
6.858LysAla: 6.858 ± 1.248
0.56LysCys: 0.56 ± 0.164
5.948LysAsp: 5.948 ± 0.967
9.937LysGlu: 9.937 ± 0.818
3.709LysPhe: 3.709 ± 0.48
6.088LysGly: 6.088 ± 0.614
1.61LysHis: 1.61 ± 0.384
8.467LysIle: 8.467 ± 0.635
10.987LysLys: 10.987 ± 1.071
6.718LysLeu: 6.718 ± 0.638
2.169LysMet: 2.169 ± 0.459
6.508LysAsn: 6.508 ± 0.759
1.889LysPro: 1.889 ± 0.384
4.689LysGln: 4.689 ± 0.724
4.409LysArg: 4.409 ± 0.6
5.528LysSer: 5.528 ± 0.617
5.948LysThr: 5.948 ± 0.745
5.878LysVal: 5.878 ± 0.732
0.63LysTrp: 0.63 ± 0.238
4.409LysTyr: 4.409 ± 0.588
0.0LysXaa: 0.0 ± 0.0
Leu
4.969LeuAla: 4.969 ± 0.726
1.12LeuCys: 1.12 ± 0.296
5.178LeuAsp: 5.178 ± 0.522
7.278LeuGlu: 7.278 ± 0.809
4.479LeuPhe: 4.479 ± 0.425
3.779LeuGly: 3.779 ± 0.454
1.26LeuHis: 1.26 ± 0.29
6.368LeuIle: 6.368 ± 0.745
10.567LeuLys: 10.567 ± 1.155
7.138LeuLeu: 7.138 ± 0.728
2.239LeuMet: 2.239 ± 0.326
6.788LeuAsn: 6.788 ± 0.703
2.729LeuPro: 2.729 ± 0.47
3.989LeuGln: 3.989 ± 0.574
4.129LeuArg: 4.129 ± 0.494
6.298LeuSer: 6.298 ± 0.748
3.499LeuThr: 3.499 ± 0.451
3.079LeuVal: 3.079 ± 0.462
1.19LeuTrp: 1.19 ± 0.267
2.099LeuTyr: 2.099 ± 0.276
0.0LeuXaa: 0.0 ± 0.0
Met
1.33MetAla: 1.33 ± 0.213
0.07MetCys: 0.07 ± 0.069
0.84MetAsp: 0.84 ± 0.222
1.749MetGlu: 1.749 ± 0.361
1.12MetPhe: 1.12 ± 0.265
1.679MetGly: 1.679 ± 0.353
0.28MetHis: 0.28 ± 0.155
1.05MetIle: 1.05 ± 0.252
3.289MetLys: 3.289 ± 0.504
3.079MetLeu: 3.079 ± 0.464
0.49MetMet: 0.49 ± 0.17
1.33MetAsn: 1.33 ± 0.334
0.91MetPro: 0.91 ± 0.255
0.98MetGln: 0.98 ± 0.25
0.49MetArg: 0.49 ± 0.229
1.54MetSer: 1.54 ± 0.267
1.889MetThr: 1.889 ± 0.366
1.12MetVal: 1.12 ± 0.292
0.07MetTrp: 0.07 ± 0.062
0.7MetTyr: 0.7 ± 0.215
0.0MetXaa: 0.0 ± 0.0
Asn
4.759AsnAla: 4.759 ± 0.845
0.35AsnCys: 0.35 ± 0.153
3.359AsnAsp: 3.359 ± 0.487
5.388AsnGlu: 5.388 ± 0.625
2.169AsnPhe: 2.169 ± 0.401
4.689AsnGly: 4.689 ± 0.6
0.7AsnHis: 0.7 ± 0.279
4.549AsnIle: 4.549 ± 0.688
6.438AsnLys: 6.438 ± 0.654
7.068AsnLeu: 7.068 ± 0.687
1.54AsnMet: 1.54 ± 0.251
4.409AsnAsn: 4.409 ± 0.577
3.079AsnPro: 3.079 ± 0.523
3.079AsnGln: 3.079 ± 0.478
2.099AsnArg: 2.099 ± 0.398
3.639AsnSer: 3.639 ± 0.39
2.799AsnThr: 2.799 ± 0.359
2.589AsnVal: 2.589 ± 0.411
0.56AsnTrp: 0.56 ± 0.161
2.239AsnTyr: 2.239 ± 0.383
0.0AsnXaa: 0.0 ± 0.0
Pro
1.33ProAla: 1.33 ± 0.317
0.14ProCys: 0.14 ± 0.096
1.889ProAsp: 1.889 ± 0.352
2.519ProGlu: 2.519 ± 0.361
1.33ProPhe: 1.33 ± 0.332
0.21ProGly: 0.21 ± 0.117
0.49ProHis: 0.49 ± 0.173
2.659ProIle: 2.659 ± 0.418
2.729ProLys: 2.729 ± 0.497
1.959ProLeu: 1.959 ± 0.478
0.28ProMet: 0.28 ± 0.137
2.659ProAsn: 2.659 ± 0.623
0.56ProPro: 0.56 ± 0.207
0.98ProGln: 0.98 ± 0.206
0.91ProArg: 0.91 ± 0.272
1.679ProSer: 1.679 ± 0.319
2.379ProThr: 2.379 ± 0.437
0.77ProVal: 0.77 ± 0.242
0.0ProTrp: 0.0 ± 0.0
1.19ProTyr: 1.19 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
2.869GlnAla: 2.869 ± 0.727
0.42GlnCys: 0.42 ± 0.273
2.239GlnAsp: 2.239 ± 0.323
3.709GlnGlu: 3.709 ± 0.459
1.19GlnPhe: 1.19 ± 0.239
2.169GlnGly: 2.169 ± 0.473
0.42GlnHis: 0.42 ± 0.182
3.499GlnIle: 3.499 ± 0.511
4.059GlnLys: 4.059 ± 0.843
3.079GlnLeu: 3.079 ± 0.573
1.54GlnMet: 1.54 ± 0.276
2.939GlnAsn: 2.939 ± 0.457
0.63GlnPro: 0.63 ± 0.235
2.379GlnGln: 2.379 ± 0.369
1.61GlnArg: 1.61 ± 0.35
2.309GlnSer: 2.309 ± 0.397
2.519GlnThr: 2.519 ± 0.306
2.519GlnVal: 2.519 ± 0.453
0.84GlnTrp: 0.84 ± 0.271
1.61GlnTyr: 1.61 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
1.679ArgAla: 1.679 ± 0.329
0.28ArgCys: 0.28 ± 0.123
1.959ArgAsp: 1.959 ± 0.335
3.429ArgGlu: 3.429 ± 0.425
2.169ArgPhe: 2.169 ± 0.437
2.029ArgGly: 2.029 ± 0.511
0.56ArgHis: 0.56 ± 0.152
3.709ArgIle: 3.709 ± 0.404
4.619ArgLys: 4.619 ± 0.615
4.129ArgLeu: 4.129 ± 0.448
1.61ArgMet: 1.61 ± 0.347
1.889ArgAsn: 1.889 ± 0.295
0.98ArgPro: 0.98 ± 0.249
1.819ArgGln: 1.819 ± 0.461
1.959ArgArg: 1.959 ± 0.32
1.749ArgSer: 1.749 ± 0.367
1.889ArgThr: 1.889 ± 0.389
2.309ArgVal: 2.309 ± 0.401
0.49ArgTrp: 0.49 ± 0.189
2.029ArgTyr: 2.029 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
3.499SerAla: 3.499 ± 0.423
0.7SerCys: 0.7 ± 0.227
2.589SerAsp: 2.589 ± 0.466
4.899SerGlu: 4.899 ± 0.553
2.799SerPhe: 2.799 ± 0.374
3.149SerGly: 3.149 ± 0.69
0.91SerHis: 0.91 ± 0.191
5.178SerIle: 5.178 ± 0.706
6.088SerLys: 6.088 ± 0.52
4.479SerLeu: 4.479 ± 0.523
1.47SerMet: 1.47 ± 0.321
3.079SerAsn: 3.079 ± 0.466
0.91SerPro: 0.91 ± 0.293
2.029SerGln: 2.029 ± 0.315
1.679SerArg: 1.679 ± 0.328
2.309SerSer: 2.309 ± 0.422
3.009SerThr: 3.009 ± 0.387
2.869SerVal: 2.869 ± 0.445
0.35SerTrp: 0.35 ± 0.148
2.379SerTyr: 2.379 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
3.569ThrAla: 3.569 ± 0.519
0.21ThrCys: 0.21 ± 0.112
3.709ThrAsp: 3.709 ± 0.428
5.388ThrGlu: 5.388 ± 0.582
3.219ThrPhe: 3.219 ± 0.517
2.869ThrGly: 2.869 ± 0.419
0.84ThrHis: 0.84 ± 0.238
3.639ThrIle: 3.639 ± 0.39
4.129ThrLys: 4.129 ± 0.488
4.899ThrLeu: 4.899 ± 0.546
0.91ThrMet: 0.91 ± 0.232
3.219ThrAsn: 3.219 ± 0.421
3.009ThrPro: 3.009 ± 0.493
2.099ThrGln: 2.099 ± 0.304
1.749ThrArg: 1.749 ± 0.315
3.149ThrSer: 3.149 ± 0.428
3.289ThrThr: 3.289 ± 0.602
3.079ThrVal: 3.079 ± 0.507
0.28ThrTrp: 0.28 ± 0.14
2.029ThrTyr: 2.029 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
2.659ValAla: 2.659 ± 0.452
0.42ValCys: 0.42 ± 0.174
2.729ValAsp: 2.729 ± 0.402
4.479ValGlu: 4.479 ± 0.626
2.379ValPhe: 2.379 ± 0.462
2.589ValGly: 2.589 ± 0.449
0.7ValHis: 0.7 ± 0.217
3.149ValIle: 3.149 ± 0.399
4.969ValLys: 4.969 ± 0.63
5.108ValLeu: 5.108 ± 0.575
0.98ValMet: 0.98 ± 0.325
3.429ValAsn: 3.429 ± 0.457
1.679ValPro: 1.679 ± 0.327
2.379ValGln: 2.379 ± 0.423
1.54ValArg: 1.54 ± 0.302
3.149ValSer: 3.149 ± 0.421
2.939ValThr: 2.939 ± 0.409
1.889ValVal: 1.889 ± 0.401
0.91ValTrp: 0.91 ± 0.275
2.379ValTyr: 2.379 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
1.05TrpAla: 1.05 ± 0.238
0.14TrpCys: 0.14 ± 0.095
0.42TrpAsp: 0.42 ± 0.18
0.91TrpGlu: 0.91 ± 0.245
0.7TrpPhe: 0.7 ± 0.201
0.35TrpGly: 0.35 ± 0.158
0.28TrpHis: 0.28 ± 0.115
0.77TrpIle: 0.77 ± 0.26
1.05TrpLys: 1.05 ± 0.216
0.77TrpLeu: 0.77 ± 0.274
0.21TrpMet: 0.21 ± 0.114
0.7TrpAsn: 0.7 ± 0.187
0.0TrpPro: 0.0 ± 0.0
0.42TrpGln: 0.42 ± 0.16
0.56TrpArg: 0.56 ± 0.179
0.56TrpSer: 0.56 ± 0.215
0.35TrpThr: 0.35 ± 0.131
1.12TrpVal: 1.12 ± 0.283
0.14TrpTrp: 0.14 ± 0.118
0.56TrpTyr: 0.56 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.309TyrAla: 2.309 ± 0.368
0.35TyrCys: 0.35 ± 0.164
2.309TyrAsp: 2.309 ± 0.411
3.359TyrGlu: 3.359 ± 0.416
2.729TyrPhe: 2.729 ± 0.359
2.659TyrGly: 2.659 ± 0.451
0.56TyrHis: 0.56 ± 0.242
2.029TyrIle: 2.029 ± 0.384
3.219TyrLys: 3.219 ± 0.367
3.779TyrLeu: 3.779 ± 0.61
0.84TyrMet: 0.84 ± 0.215
2.869TyrAsn: 2.869 ± 0.435
1.61TyrPro: 1.61 ± 0.313
2.169TyrGln: 2.169 ± 0.381
2.169TyrArg: 2.169 ± 0.37
1.819TyrSer: 1.819 ± 0.326
2.029TyrThr: 2.029 ± 0.46
1.26TyrVal: 1.26 ± 0.268
0.35TyrTrp: 0.35 ± 0.141
1.749TyrTyr: 1.749 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (14291 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski