Rhodovulum sp. ES.010

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum; unclassified Rhodovulum

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6KTD0|A0A1N6KTD0_9RHOB dTDP-glucose 4 6-dehydratase OS=Rhodovulum sp. ES.010 OX=1882821 GN=SAMN05444722_3775 PE=3 SV=1
MM1 pKa = 7.58TSLKK5 pKa = 10.32YY6 pKa = 10.25LVAGAVFAASAGVASAAPVLYY27 pKa = 10.44ADD29 pKa = 5.22AVLDD33 pKa = 4.17VNKK36 pKa = 8.76GTCVAQAGIGPLDD49 pKa = 4.08GDD51 pKa = 3.61NCAVNRR57 pKa = 11.84DD58 pKa = 3.98DD59 pKa = 5.31PLAALGAPDD68 pKa = 3.86GDD70 pKa = 4.61FYY72 pKa = 12.02ALGFGGDD79 pKa = 3.12ITLGFSGSPFPGGEE93 pKa = 3.99TTVYY97 pKa = 10.42EE98 pKa = 4.37VTFDD102 pKa = 3.24RR103 pKa = 11.84TVDD106 pKa = 3.13HH107 pKa = 7.62DD108 pKa = 4.23EE109 pKa = 4.63AVDD112 pKa = 4.52LYY114 pKa = 11.44SVLGGVATYY123 pKa = 10.61VATIFNSPTGTGTALISGDD142 pKa = 3.49FDD144 pKa = 4.65SIKK147 pKa = 10.78LVDD150 pKa = 3.47VTADD154 pKa = 3.29YY155 pKa = 10.34FAIEE159 pKa = 4.27NDD161 pKa = 3.4GDD163 pKa = 3.95PATNLSFDD171 pKa = 4.0GFDD174 pKa = 3.24VAAVGLAPIPLPAAGLMLLAGLGGLGALRR203 pKa = 11.84RR204 pKa = 11.84KK205 pKa = 10.11SSS207 pKa = 2.96

Molecular weight: 20.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6JDY4|A0A1N6JDY4_9RHOB Nitric oxide reductase NorQ protein OS=Rhodovulum sp. ES.010 OX=1882821 GN=SAMN05444722_2065 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3606

0

3606

1120362

25

3334

310.7

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.719 ± 0.069

0.877 ± 0.016

5.966 ± 0.034

6.21 ± 0.04

3.573 ± 0.029

9.075 ± 0.04

2.016 ± 0.019

4.554 ± 0.026

2.557 ± 0.032

10.267 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.021

2.108 ± 0.016

5.485 ± 0.032

2.714 ± 0.02

7.72 ± 0.049

4.476 ± 0.027

5.206 ± 0.027

7.391 ± 0.035

1.4 ± 0.02

2.093 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski