Streptococcus phage Javan132

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AUQ5|A0A4D6AUQ5_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan132 OX=2547996 GN=Javan132_0058 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.52RR3 pKa = 11.84GLFGTFDD10 pKa = 3.62YY11 pKa = 11.26DD12 pKa = 3.68RR13 pKa = 11.84DD14 pKa = 3.93YY15 pKa = 11.4LQPEE19 pKa = 4.41PEE21 pKa = 4.11RR22 pKa = 11.84EE23 pKa = 3.91EE24 pKa = 4.35RR25 pKa = 11.84DD26 pKa = 3.18PDD28 pKa = 2.91EE29 pKa = 3.78WLFRR33 pKa = 11.84GGQWIYY39 pKa = 11.19VGDD42 pKa = 3.89YY43 pKa = 10.77

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AQZ3|A0A4D6AQZ3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan132 OX=2547996 GN=Javan132_0065 PE=4 SV=1
MM1 pKa = 7.41TEE3 pKa = 3.98SKK5 pKa = 10.56RR6 pKa = 11.84VGRR9 pKa = 11.84PKK11 pKa = 10.57SEE13 pKa = 3.53NSKK16 pKa = 9.71NRR18 pKa = 11.84KK19 pKa = 5.67VTVRR23 pKa = 11.84MTEE26 pKa = 3.9QQFHH30 pKa = 6.27KK31 pKa = 10.64LEE33 pKa = 4.13RR34 pKa = 11.84VANKK38 pKa = 10.04VNLTKK43 pKa = 10.6TEE45 pKa = 4.26TILRR49 pKa = 11.84GIDD52 pKa = 3.63LQDD55 pKa = 3.39TEE57 pKa = 4.27QQ58 pKa = 3.82

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12695

40

1307

186.7

21.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.837 ± 0.667

0.559 ± 0.12

6.593 ± 0.311

7.42 ± 0.272

3.75 ± 0.241

6.239 ± 0.286

1.339 ± 0.124

7.05 ± 0.311

9.311 ± 0.292

8.885 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.153

5.916 ± 0.231

2.67 ± 0.181

4.072 ± 0.191

4.214 ± 0.194

6.128 ± 0.292

5.931 ± 0.229

5.908 ± 0.25

1.119 ± 0.097

3.702 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski