Paenibacillus hemerocallicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C4SV45|A0A5C4SV45_9BACL IS3 family transposase OS=Paenibacillus hemerocallicola OX=1172614 GN=FE784_40510 PE=4 SV=1
MM1 pKa = 7.38PAIVGNITILSVGPSSIVHH20 pKa = 6.38IGDD23 pKa = 5.4AIQLAPQSTSKK34 pKa = 9.6TFAGAGSFNTGDD46 pKa = 3.62AVQTYY51 pKa = 10.11NAVSSTNTYY60 pKa = 10.62DD61 pKa = 5.12ADD63 pKa = 3.7IADD66 pKa = 4.32TNAVSANGAGVLL78 pKa = 4.0

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C4SXA0|A0A5C4SXA0_9BACL PNP_UDP_1 domain-containing protein OS=Paenibacillus hemerocallicola OX=1172614 GN=FE784_35730 PE=4 SV=1
MM1 pKa = 7.51FNRR4 pKa = 11.84NVRR7 pKa = 11.84VTFFSGFNFTRR18 pKa = 11.84RR19 pKa = 11.84SLTFRR24 pKa = 11.84RR25 pKa = 11.84GVAVSNLGLFGFNNIISSFRR45 pKa = 11.84LRR47 pKa = 11.84NVVIPSQVTLVLFSGRR63 pKa = 11.84NFTGNFRR70 pKa = 11.84TFRR73 pKa = 11.84GSQNISDD80 pKa = 3.43LRR82 pKa = 11.84AFNFNNVTSSFILVGFRR99 pKa = 11.84ITTSQIRR106 pKa = 11.84TIQRR110 pKa = 11.84TGRR113 pKa = 11.84MPSGISKK120 pKa = 10.35LL121 pKa = 3.71

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7848

0

7848

2694558

19

3154

343.3

38.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.8 ± 0.033

0.828 ± 0.01

5.367 ± 0.017

6.495 ± 0.032

4.07 ± 0.018

7.869 ± 0.027

2.104 ± 0.015

6.02 ± 0.021

5.04 ± 0.025

9.698 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.013

3.646 ± 0.022

4.403 ± 0.018

3.507 ± 0.016

5.827 ± 0.03

6.244 ± 0.02

5.404 ± 0.028

7.044 ± 0.023

1.343 ± 0.011

3.566 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski