Chengkuizengella sp. YPA3-1-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Chengkuizengella

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N9Q360|A0A6N9Q360_9BACL SLC13 family permease OS=Chengkuizengella sp. YPA3-1-1 OX=2507566 GN=ERL59_09725 PE=4 SV=1
MM1 pKa = 8.31DD2 pKa = 6.26DD3 pKa = 3.53FNEE6 pKa = 4.67WIQWNANQNDD16 pKa = 4.0LEE18 pKa = 4.19QAIVRR23 pKa = 11.84NGGTYY28 pKa = 10.26NLEE31 pKa = 4.43GNTNLYY37 pKa = 10.86FFFNGGNTPEE47 pKa = 5.21SFDD50 pKa = 3.36QFINRR55 pKa = 11.84DD56 pKa = 2.95ISDD59 pKa = 4.02YY60 pKa = 10.53DD61 pKa = 3.66TLEE64 pKa = 4.17LVLKK68 pKa = 9.92GGSGGEE74 pKa = 3.67EE75 pKa = 3.79QYY77 pKa = 11.5INLVLNDD84 pKa = 4.63DD85 pKa = 4.55SNHH88 pKa = 5.87SLSLSNYY95 pKa = 9.32GNLTANYY102 pKa = 8.45STIQIPLADD111 pKa = 4.64FNANLTNVKK120 pKa = 9.99FIRR123 pKa = 11.84FEE125 pKa = 4.19GVGTAKK131 pKa = 10.12IVRR134 pKa = 11.84IDD136 pKa = 4.45DD137 pKa = 4.29MILKK141 pKa = 8.28YY142 pKa = 9.48TGISPTDD149 pKa = 3.52PTDD152 pKa = 3.59PTDD155 pKa = 3.84PTDD158 pKa = 3.84PTDD161 pKa = 3.84PTDD164 pKa = 3.77PTDD167 pKa = 3.62PTEE170 pKa = 4.48PTDD173 pKa = 3.61PTDD176 pKa = 4.2PGDD179 pKa = 3.74VPDD182 pKa = 5.09FPVGSGVMTFQLQNGTNGQYY202 pKa = 10.66SDD204 pKa = 5.6DD205 pKa = 4.14EE206 pKa = 4.86IYY208 pKa = 9.79WAIYY212 pKa = 9.62GYY214 pKa = 10.82NKK216 pKa = 8.71EE217 pKa = 4.4TGQLSYY223 pKa = 11.68VDD225 pKa = 4.32ANGNLIASSVSDD237 pKa = 3.5NDD239 pKa = 4.13APGHH243 pKa = 4.79LTKK246 pKa = 10.98NGEE249 pKa = 3.85NYY251 pKa = 10.87ANYY254 pKa = 8.71FNKK257 pKa = 9.65MSEE260 pKa = 4.29TDD262 pKa = 3.37WVSMPEE268 pKa = 3.8IDD270 pKa = 3.83SGRR273 pKa = 11.84MFISMGSPMYY283 pKa = 10.41IKK285 pKa = 10.82LNIAADD291 pKa = 3.79GRR293 pKa = 11.84VGFAGPDD300 pKa = 3.57LNNPTDD306 pKa = 4.35PNQDD310 pKa = 2.95IYY312 pKa = 11.27FEE314 pKa = 4.68WIEE317 pKa = 3.87FTIDD321 pKa = 2.47QWGYY325 pKa = 9.66HH326 pKa = 5.54GNTTRR331 pKa = 11.84VDD333 pKa = 3.42QFSFPITTRR342 pKa = 11.84LVASDD347 pKa = 4.02GYY349 pKa = 11.26DD350 pKa = 3.15RR351 pKa = 11.84IVGEE355 pKa = 4.61TYY357 pKa = 10.18TRR359 pKa = 11.84DD360 pKa = 3.48QIFTAFKK367 pKa = 11.04NEE369 pKa = 4.04MPTEE373 pKa = 4.03FQTLVEE379 pKa = 4.09EE380 pKa = 5.25PYY382 pKa = 10.63RR383 pKa = 11.84IVAPGKK389 pKa = 10.99GEE391 pKa = 4.11FKK393 pKa = 10.88PEE395 pKa = 4.16GIYY398 pKa = 10.98ANYY401 pKa = 9.33FDD403 pKa = 5.43NYY405 pKa = 9.92VNEE408 pKa = 3.76VWDD411 pKa = 4.9FYY413 pKa = 10.83RR414 pKa = 11.84TNEE417 pKa = 3.9LTFTAEE423 pKa = 3.71AGTFSGHH430 pKa = 5.19VVGDD434 pKa = 3.54DD435 pKa = 3.78FVFSKK440 pKa = 11.18NGGPYY445 pKa = 10.72DD446 pKa = 3.51LFIRR450 pKa = 11.84NKK452 pKa = 8.64PTTLQVFEE460 pKa = 5.34CSGPMDD466 pKa = 3.84TGSPDD471 pKa = 3.43EE472 pKa = 4.71KK473 pKa = 10.87VVEE476 pKa = 4.34AQVCAALNRR485 pKa = 11.84GMMYY489 pKa = 10.78DD490 pKa = 3.56PANWGNSDD498 pKa = 4.75FFYY501 pKa = 11.18QNNTANFYY509 pKa = 11.17AKK511 pKa = 10.03FWHH514 pKa = 7.41DD515 pKa = 3.48YY516 pKa = 10.93SIDD519 pKa = 3.24GLAYY523 pKa = 9.77GFAYY527 pKa = 10.48DD528 pKa = 4.64DD529 pKa = 3.55VRR531 pKa = 11.84NYY533 pKa = 9.29STLLEE538 pKa = 4.21HH539 pKa = 7.37HH540 pKa = 7.36DD541 pKa = 4.04PDD543 pKa = 3.84VLFINVGWW551 pKa = 3.67

Molecular weight: 62.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N9Q307|A0A6N9Q307_9BACL Membrane protein insertase YidC OS=Chengkuizengella sp. YPA3-1-1 OX=2507566 GN=yidC PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.72PTFQPNTRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84KK13 pKa = 8.71KK14 pKa = 8.74VHH16 pKa = 5.67GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MNSKK25 pKa = 10.17NGRR28 pKa = 11.84KK29 pKa = 9.32VLTARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.12GRR39 pKa = 11.84KK40 pKa = 8.67ILSAA44 pKa = 4.02

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3947

0

3947

1225611

18

5234

310.5

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.582 ± 0.042

0.773 ± 0.016

5.466 ± 0.036

7.522 ± 0.048

4.548 ± 0.036

6.362 ± 0.038

2.072 ± 0.02

8.69 ± 0.041

7.157 ± 0.044

9.467 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.018

5.45 ± 0.04

3.388 ± 0.021

3.877 ± 0.029

3.533 ± 0.027

6.485 ± 0.034

5.437 ± 0.036

6.653 ± 0.031

1.071 ± 0.016

3.828 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski