Phascolarctobacterium faecium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6I9W5|R6I9W5_9FIRM Pyridine nucleotide-disulfide oxidoreductase OS=Phascolarctobacterium faecium OX=33025 GN=BN533_01142 PE=4 SV=1
MM1 pKa = 6.99KK2 pKa = 9.81TFRR5 pKa = 11.84EE6 pKa = 4.03QIVADD11 pKa = 3.62NTAAFINFLEE21 pKa = 4.58FAEE24 pKa = 4.08EE25 pKa = 4.15HH26 pKa = 6.1NLNGILCDD34 pKa = 5.4AIIQDD39 pKa = 3.53VSIAEE44 pKa = 4.24SLSTGNGADD53 pKa = 3.16QTYY56 pKa = 10.71AGIYY60 pKa = 9.95GSRR63 pKa = 11.84LQINCLAEE71 pKa = 4.37ALPEE75 pKa = 4.1LPVYY79 pKa = 10.28GQLFGIDD86 pKa = 3.39DD87 pKa = 3.76KK88 pKa = 11.68QYY90 pKa = 11.18LVEE93 pKa = 4.45SCADD97 pKa = 3.34DD98 pKa = 3.58MGILTIQLVANDD110 pKa = 3.71RR111 pKa = 3.81

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G9H5N2|A0A3G9H5N2_9FIRM HIT domain-containing protein OS=Phascolarctobacterium faecium OX=33025 GN=hinT PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.78RR14 pKa = 11.84THH16 pKa = 6.12GFRR19 pKa = 11.84EE20 pKa = 3.88RR21 pKa = 11.84MKK23 pKa = 10.27TLGGRR28 pKa = 11.84QVLKK32 pKa = 10.37RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.99KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2228

0

2228

694653

29

1597

311.8

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.078 ± 0.06

1.37 ± 0.024

5.262 ± 0.037

6.557 ± 0.06

3.993 ± 0.039

7.613 ± 0.061

1.726 ± 0.022

7.039 ± 0.042

6.299 ± 0.051

9.99 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.027

4.251 ± 0.053

3.538 ± 0.036

3.466 ± 0.032

4.397 ± 0.041

5.416 ± 0.043

5.266 ± 0.038

7.342 ± 0.045

0.93 ± 0.018

3.535 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski