Roseovarius sp. A46

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius; unclassified Roseovarius

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2A2L6|A0A4Q2A2L6_9RHOB Flagellar motor switch protein FliM OS=Roseovarius sp. A46 OX=2109331 GN=C6W92_10615 PE=4 SV=1
MM1 pKa = 7.7AAPASAQEE9 pKa = 4.35WNMKK13 pKa = 8.87WGGFANTHH21 pKa = 5.31VGITDD26 pKa = 3.23VSGTGVTGADD36 pKa = 3.34FDD38 pKa = 4.55GVDD41 pKa = 3.28VYY43 pKa = 11.25TNSEE47 pKa = 4.3IIFSPSVTLDD57 pKa = 2.77NGMTFGFSVQMEE69 pKa = 4.11AMNNGGGAVGIDD81 pKa = 3.41EE82 pKa = 4.85SYY84 pKa = 9.74VTIEE88 pKa = 3.97SDD90 pKa = 3.45TLGKK94 pKa = 10.42VVLGSEE100 pKa = 3.98NSAGYY105 pKa = 10.9LLMGSVYY112 pKa = 9.54TPGVSSMAINSRR124 pKa = 11.84SLSAFIPVTGSGGFRR139 pKa = 11.84QAALSSLTEE148 pKa = 3.76VAGNNDD154 pKa = 3.51VQRR157 pKa = 11.84ITYY160 pKa = 7.29FTPSFNGLTLGASYY174 pKa = 10.88AATSAGNTTNNFGVNRR190 pKa = 11.84NAVGPVFDD198 pKa = 4.99IFDD201 pKa = 3.55IGAKK205 pKa = 9.97YY206 pKa = 9.8SQSFNGVDD214 pKa = 2.97VGLAARR220 pKa = 11.84WGTGDD225 pKa = 3.27TNTVGRR231 pKa = 11.84SDD233 pKa = 3.5PTTWGVGGTIGVSGFTFGATYY254 pKa = 10.99AEE256 pKa = 4.28NDD258 pKa = 3.12NGFAGGAGDD267 pKa = 3.76QEE269 pKa = 4.74GWSVGASYY277 pKa = 10.67DD278 pKa = 3.33IAGPWTVGLSTYY290 pKa = 10.29QGEE293 pKa = 4.39TSNGGGAPSSDD304 pKa = 3.42YY305 pKa = 10.87EE306 pKa = 4.57AYY308 pKa = 10.95LLGASRR314 pKa = 11.84SLGAGVSWDD323 pKa = 3.12VYY325 pKa = 8.28YY326 pKa = 10.99AYY328 pKa = 10.21MEE330 pKa = 4.48TNNGAVAGNPGIDD343 pKa = 3.59GNLLATAINLSFF355 pKa = 4.39

Molecular weight: 36.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1ZZA7|A0A4Q1ZZA7_9RHOB GTP 3' 8-cyclase OS=Roseovarius sp. A46 OX=2109331 GN=moaA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3369

0

3369

1039432

28

1788

308.5

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.874 ± 0.069

0.936 ± 0.015

6.061 ± 0.037

6.066 ± 0.039

3.538 ± 0.024

9.075 ± 0.039

2.197 ± 0.021

4.908 ± 0.031

2.63 ± 0.036

10.011 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.806 ± 0.022

2.308 ± 0.029

5.203 ± 0.029

2.847 ± 0.02

7.562 ± 0.042

4.614 ± 0.028

5.382 ± 0.029

7.39 ± 0.037

1.41 ± 0.018

2.182 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski