Capybara microvirus Cap3_SP_469
Average proteome isoelectric point is 5.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8P9|A0A4P8W8P9_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_469 OX=2585464 PE=4 SV=1
MM1 pKa = 7.37 SKK3 pKa = 10.61 KK4 pKa = 10.41 KK5 pKa = 10.42 VVNDD9 pKa = 3.64 LSYY12 pKa = 10.3 HH13 pKa = 4.53 YY14 pKa = 10.38 HH15 pKa = 6.64 VNTCDD20 pKa = 3.16 EE21 pKa = 5.08 GINEE25 pKa = 4.24 VKK27 pKa = 10.43 CACKK31 pKa = 10.21 LYY33 pKa = 11.14 NEE35 pKa = 4.53 GKK37 pKa = 8.5 YY38 pKa = 8.39 TFEE41 pKa = 4.05 EE42 pKa = 5.06 LILFLNTIKK51 pKa = 9.37 TQIDD55 pKa = 3.98 DD56 pKa = 4.86 DD57 pKa = 5.23 LDD59 pKa = 4.15 DD60 pKa = 6.18 LILEE64 pKa = 4.52 VICDD68 pKa = 4.19 RR69 pKa = 11.84 IEE71 pKa = 4.38 KK72 pKa = 10.17 IEE74 pKa = 4.25 SEE76 pKa = 4.65 VYY78 pKa = 10.73 KK79 pKa = 11.1 NN80 pKa = 3.27
Molecular weight: 9.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.521
IPC2_protein 4.685
IPC_protein 4.558
Toseland 4.406
ProMoST 4.673
Dawson 4.507
Bjellqvist 4.66
Wikipedia 4.393
Rodwell 4.406
Grimsley 4.317
Solomon 4.507
Lehninger 4.457
Nozaki 4.622
DTASelect 4.774
Thurlkill 4.418
EMBOSS 4.406
Sillero 4.673
Patrickios 3.3
IPC_peptide 4.507
IPC2_peptide 4.66
IPC2.peptide.svr19 4.63
Protein with the highest isoelectric point:
>tr|A0A4P8W549|A0A4P8W549_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_469 OX=2585464 PE=3 SV=1
MM1 pKa = 7.14 CTSPLKK7 pKa = 10.52 GFPYY11 pKa = 10.83 GKK13 pKa = 8.58 TKK15 pKa = 10.58 NGKK18 pKa = 9.25 DD19 pKa = 3.17 NFVITSYY26 pKa = 10.89 KK27 pKa = 9.68 CDD29 pKa = 3.95 HH30 pKa = 6.83 IEE32 pKa = 3.92 VNRR35 pKa = 11.84 NDD37 pKa = 3.81 VPIKK41 pKa = 10.1 IYY43 pKa = 10.58 EE44 pKa = 3.92 PTFIHH49 pKa = 6.62 SQDD52 pKa = 3.16 TKK54 pKa = 11.02 EE55 pKa = 4.24 IIRR58 pKa = 11.84 DD59 pKa = 4.41 FIEE62 pKa = 4.66 IPCGHH67 pKa = 6.86 CLEE70 pKa = 4.91 CRR72 pKa = 11.84 LDD74 pKa = 4.59 YY75 pKa = 10.96 SRR77 pKa = 11.84 QWANRR82 pKa = 11.84 CMLEE86 pKa = 4.07 AQSYY90 pKa = 7.16 KK91 pKa = 10.52 HH92 pKa = 6.32 NYY94 pKa = 8.68 FVTLTYY100 pKa = 11.11 DD101 pKa = 4.05 DD102 pKa = 4.4 LHH104 pKa = 8.53 LPMNEE109 pKa = 3.96 YY110 pKa = 10.92 VDD112 pKa = 3.7 VDD114 pKa = 3.77 GVLQLSPTLVKK125 pKa = 10.28 RR126 pKa = 11.84 DD127 pKa = 3.43 LQLFMKK133 pKa = 10.54 RR134 pKa = 11.84 LRR136 pKa = 11.84 KK137 pKa = 9.97 KK138 pKa = 10.27 FGDD141 pKa = 3.44 NIRR144 pKa = 11.84 FYY146 pKa = 11.58 ACGEE150 pKa = 4.29 YY151 pKa = 10.59 GSKK154 pKa = 7.16 THH156 pKa = 6.81 RR157 pKa = 11.84 PHH159 pKa = 5.64 YY160 pKa = 10.36 HH161 pKa = 6.98 LILFNFNPNDD171 pKa = 5.11 LIFLKK176 pKa = 10.91 QNFQGDD182 pKa = 4.44 LYY184 pKa = 8.88 YY185 pKa = 10.47 TSPGLEE191 pKa = 4.18 SCWKK195 pKa = 9.83 YY196 pKa = 10.38 GYY198 pKa = 11.62 SMIAEE203 pKa = 4.53 VNWNTCAYY211 pKa = 7.52 VARR214 pKa = 11.84 YY215 pKa = 8.62 IVKK218 pKa = 10.03 KK219 pKa = 10.64 QFGEE223 pKa = 3.81 NADD226 pKa = 3.63 YY227 pKa = 11.25 KK228 pKa = 10.66 KK229 pKa = 10.87 FNYY232 pKa = 9.77 EE233 pKa = 3.81 PEE235 pKa = 4.12 FTLMSRR241 pKa = 11.84 KK242 pKa = 9.57 PGIARR247 pKa = 11.84 DD248 pKa = 3.23 WYY250 pKa = 10.29 EE251 pKa = 3.63 NHH253 pKa = 7.3 KK254 pKa = 11.15 DD255 pKa = 3.39 NLYY258 pKa = 10.73 DD259 pKa = 3.6 YY260 pKa = 10.79 DD261 pKa = 5.68 AIYY264 pKa = 10.32 IKK266 pKa = 10.67 DD267 pKa = 4.61 GIPVKK272 pKa = 9.93 PSKK275 pKa = 11.1 YY276 pKa = 9.74 FDD278 pKa = 3.35 RR279 pKa = 11.84 LYY281 pKa = 10.86 DD282 pKa = 3.38 IDD284 pKa = 4.94 YY285 pKa = 9.04 SAQMEE290 pKa = 4.61 EE291 pKa = 3.87 IKK293 pKa = 10.52 KK294 pKa = 10.0 VRR296 pKa = 11.84 LEE298 pKa = 4.1 SMLHH302 pKa = 5.36 HH303 pKa = 6.89 KK304 pKa = 9.78 MLIAEE309 pKa = 4.54 MTSQTYY315 pKa = 9.9 EE316 pKa = 4.05 EE317 pKa = 4.23 QLSTRR322 pKa = 11.84 NNVIEE327 pKa = 4.46 EE328 pKa = 4.14 KK329 pKa = 9.98 TKK331 pKa = 9.84 KK332 pKa = 10.13 LKK334 pKa = 10.55 RR335 pKa = 11.84 KK336 pKa = 9.45 EE337 pKa = 3.68 II338 pKa = 3.99
Molecular weight: 40.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.023
IPC2_protein 7.322
IPC_protein 7.22
Toseland 6.971
ProMoST 7.761
Dawson 7.892
Bjellqvist 8.2
Wikipedia 7.819
Rodwell 7.907
Grimsley 7.044
Solomon 7.966
Lehninger 7.98
Nozaki 8.331
DTASelect 8.009
Thurlkill 8.053
EMBOSS 8.126
Sillero 8.346
Patrickios 3.834
IPC_peptide 7.951
IPC2_peptide 7.293
IPC2.peptide.svr19 7.383
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1444
80
552
240.7
27.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.055 ± 1.099
1.316 ± 0.584
5.886 ± 0.785
5.748 ± 1.157
4.571 ± 0.572
7.064 ± 1.283
1.87 ± 0.487
5.886 ± 0.87
5.956 ± 1.637
7.964 ± 0.45
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.77 ± 0.6
8.102 ± 1.357
4.155 ± 0.802
3.463 ± 0.393
3.67 ± 0.709
8.657 ± 1.706
5.471 ± 0.264
5.956 ± 0.6
1.108 ± 0.248
5.332 ± 0.784
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here