Salegentibacter echinorum
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3479 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5FDQ3|A0A1M5FDQ3_9FLAO Acyl carrier protein phosphodiesterase OS=Salegentibacter echinorum OX=1073325 GN=SAMN05444483_103148 PE=4 SV=1
MM1 pKa = 7.35 NKK3 pKa = 9.44 YY4 pKa = 10.05 HH5 pKa = 6.93 SLPTGPNFRR14 pKa = 11.84 YY15 pKa = 9.57 LPKK18 pKa = 10.57 GLYY21 pKa = 8.97 PLLFIFFFLLKK32 pKa = 10.29 SGVGFGQEE40 pKa = 3.65 NCIITSTATDD50 pKa = 3.7 PTEE53 pKa = 4.06 TSPIPLNMKK62 pKa = 10.3 FEE64 pKa = 4.37 GSINRR69 pKa = 11.84 QLDD72 pKa = 3.36 EE73 pKa = 5.5 DD74 pKa = 4.6 DD75 pKa = 5.54 FIVSNGNIGNFEE87 pKa = 4.21 RR88 pKa = 11.84 GVPEE92 pKa = 3.91 FTKK95 pKa = 10.52 SRR97 pKa = 11.84 EE98 pKa = 4.18 DD99 pKa = 3.32 IEE101 pKa = 4.47 IEE103 pKa = 3.89 NFQVEE108 pKa = 4.75 GSAFEE113 pKa = 4.15 IFIKK117 pKa = 10.66 GISEE121 pKa = 3.99 FLAEE125 pKa = 4.26 EE126 pKa = 4.26 FGDD129 pKa = 3.84 AVISIAHH136 pKa = 4.66 YY137 pKa = 9.68 TKK139 pKa = 11.13 DD140 pKa = 3.14 NLFYY144 pKa = 10.84 LSYY147 pKa = 10.97 SNGVKK152 pKa = 10.25 SLNLVTEE159 pKa = 4.82 NKK161 pKa = 9.84 QSTSITGDD169 pKa = 3.69 DD170 pKa = 4.34 LNSPLDD176 pKa = 3.6 IEE178 pKa = 5.04 ASLSGVDD185 pKa = 3.44 QRR187 pKa = 11.84 LYY189 pKa = 10.99 LADD192 pKa = 3.77 KK193 pKa = 10.73 NKK195 pKa = 10.0 QQVNVYY201 pKa = 10.67 NNATVKK207 pKa = 10.73 LDD209 pKa = 3.59 DD210 pKa = 4.51 FKK212 pKa = 11.82 VPNYY216 pKa = 10.37 DD217 pKa = 2.89 SSNDD221 pKa = 3.48 VGPTGIVTDD230 pKa = 4.5 DD231 pKa = 4.3 DD232 pKa = 4.52 GNIYY236 pKa = 8.7 VTVAFTPDD244 pKa = 2.63 GTYY247 pKa = 10.64 DD248 pKa = 3.4 VEE250 pKa = 4.15 YY251 pKa = 11.06 DD252 pKa = 3.21 KK253 pKa = 11.7 VAVYY257 pKa = 10.47 KK258 pKa = 10.37 PGNYY262 pKa = 9.67 NNPTVIPADD271 pKa = 3.56 NSGLQLDD278 pKa = 4.21 RR279 pKa = 11.84 PYY281 pKa = 10.87 RR282 pKa = 11.84 IAVDD286 pKa = 3.45 KK287 pKa = 11.09 NGRR290 pKa = 11.84 IYY292 pKa = 10.47 IADD295 pKa = 4.0 GGGSTPNGRR304 pKa = 11.84 VLVFDD309 pKa = 4.0 EE310 pKa = 5.03 NYY312 pKa = 10.58 NHH314 pKa = 6.73 IHH316 pKa = 6.29 TIEE319 pKa = 4.21 GTQDD323 pKa = 3.06 KK324 pKa = 10.26 IGAPGSIIVDD334 pKa = 3.51 DD335 pKa = 4.6 FGYY338 pKa = 10.26 IYY340 pKa = 10.16 IIDD343 pKa = 4.06 YY344 pKa = 11.19 QNDD347 pKa = 2.91 ITFNGIFQNPEE358 pKa = 4.03 DD359 pKa = 4.78 LLGEE363 pKa = 3.97 YY364 pKa = 10.52 DD365 pKa = 4.37 KK366 pKa = 11.06 IISTSYY372 pKa = 10.41 SVNVYY377 pKa = 10.56 DD378 pKa = 4.4 SNANFEE384 pKa = 4.53 YY385 pKa = 9.95 VTEE388 pKa = 4.76 FNQGLNLPIDD398 pKa = 4.15 LEE400 pKa = 3.94 IDD402 pKa = 3.44 YY403 pKa = 10.98 CGNILVNNLKK413 pKa = 10.7 LSGPAPEE420 pKa = 4.5 VLTGIDD426 pKa = 3.29 INFDD430 pKa = 3.55 FQLKK434 pKa = 8.48 TFTRR438 pKa = 11.84 HH439 pKa = 6.73 DD440 pKa = 3.73 NFTAEE445 pKa = 4.06 VTPNGEE451 pKa = 3.99 GLVEE455 pKa = 4.13 VSLNPEE461 pKa = 3.96 NEE463 pKa = 4.21 FFPCDD468 pKa = 3.82 PQPEE472 pKa = 4.37 CSFSIEE478 pKa = 4.44 YY479 pKa = 8.48 KK480 pKa = 10.27 TEE482 pKa = 4.02 AAPDD486 pKa = 3.79 PTFDD490 pKa = 4.58 CPIEE494 pKa = 4.5 SEE496 pKa = 4.25 IDD498 pKa = 3.23 PLFYY502 pKa = 11.16 GNNCSVSVPDD512 pKa = 4.12 YY513 pKa = 10.66 EE514 pKa = 4.79 ISNPQHH520 pKa = 6.38 FEE522 pKa = 3.72 SDD524 pKa = 3.24 PFLVQTYY531 pKa = 8.11 TEE533 pKa = 4.39 NEE535 pKa = 3.9 NSVSVLLKK543 pKa = 10.88 VYY545 pKa = 10.58 DD546 pKa = 4.03 GEE548 pKa = 4.9 NGDD551 pKa = 6.22 LVDD554 pKa = 3.65 EE555 pKa = 5.11 CNFEE559 pKa = 4.78 VDD561 pKa = 5.54 LIDD564 pKa = 3.95 NKK566 pKa = 11.14 APVISDD572 pKa = 4.12 CPPEE576 pKa = 4.45 NIQYY580 pKa = 8.54 TIQEE584 pKa = 4.67 GEE586 pKa = 4.07 THH588 pKa = 6.83 ILDD591 pKa = 5.28 DD592 pKa = 4.0 YY593 pKa = 10.93 TDD595 pKa = 3.77 QISVNDD601 pKa = 3.85 NCDD604 pKa = 3.62 SNPTIVQDD612 pKa = 4.01 PPAGTEE618 pKa = 3.53 ITADD622 pKa = 3.36 QSVTLIVTDD631 pKa = 3.62 EE632 pKa = 4.4 ADD634 pKa = 4.94 NEE636 pKa = 4.61 TEE638 pKa = 4.34 CNFKK642 pKa = 11.16 VEE644 pKa = 4.17 ITEE647 pKa = 4.16 VAVPDD652 pKa = 3.93 PTFDD656 pKa = 4.36 CPVEE660 pKa = 4.26 NNLDD664 pKa = 3.69 TIYY667 pKa = 11.3 YY668 pKa = 10.37 DD669 pKa = 4.71 DD670 pKa = 3.97 NCSYY674 pKa = 11.17 NVPKK678 pKa = 10.88 YY679 pKa = 10.55 KK680 pKa = 10.17 ISNPQHH686 pKa = 6.69 FDD688 pKa = 3.33 PEE690 pKa = 4.5 PYY692 pKa = 10.4 LIQSHH697 pKa = 5.72 TEE699 pKa = 3.78 NGDD702 pKa = 3.87 SVSVTIKK709 pKa = 10.44 VYY711 pKa = 10.42 NGEE714 pKa = 3.96 GGEE717 pKa = 4.46 LVDD720 pKa = 3.66 ICDD723 pKa = 3.99 FEE725 pKa = 4.82 VALEE729 pKa = 4.24 DD730 pKa = 3.46 NQAPVISGCPPEE742 pKa = 5.48 NIQFTIQEE750 pKa = 4.64 GEE752 pKa = 4.07 THH754 pKa = 6.89 ILDD757 pKa = 5.17 DD758 pKa = 3.82 YY759 pKa = 10.65 TGQISVNDD767 pKa = 3.78 NCDD770 pKa = 3.69 SNPSIVQDD778 pKa = 3.89 PPAGTEE784 pKa = 3.52 ITADD788 pKa = 3.5 RR789 pKa = 11.84 LVTFTVTDD797 pKa = 3.48 KK798 pKa = 11.44 AGNEE802 pKa = 4.27 TEE804 pKa = 4.35 CNFMVEE810 pKa = 4.15 ITEE813 pKa = 4.36 EE814 pKa = 4.31 EE815 pKa = 4.83 PNTAPVAADD824 pKa = 3.19 NTYY827 pKa = 10.84 SIDD830 pKa = 3.61 QNEE833 pKa = 4.76 VLTIPAPGILQNDD846 pKa = 3.89 TDD848 pKa = 4.79 SEE850 pKa = 4.47 DD851 pKa = 4.39 DD852 pKa = 3.88 NLTASLVTNVSNGTLTFNADD872 pKa = 2.77 GSFTYY877 pKa = 10.09 TPNTGFIGSDD887 pKa = 2.81 SFTYY891 pKa = 10.03 KK892 pKa = 10.96 ANDD895 pKa = 3.75 GEE897 pKa = 4.48 LDD899 pKa = 3.87 SNIATVTINVNSITAPNTAPVAADD923 pKa = 3.19 NTYY926 pKa = 10.84 SIDD929 pKa = 3.61 QNEE932 pKa = 4.76 VLTIPAPGILQNDD945 pKa = 3.79 TDD947 pKa = 4.37 SEE949 pKa = 4.33 EE950 pKa = 4.91 DD951 pKa = 3.53 NLTASLDD958 pKa = 3.74 SDD960 pKa = 4.02 VSNGTLTFNADD971 pKa = 2.81 GSFTYY976 pKa = 10.25 IPNSDD981 pKa = 3.88 FKK983 pKa = 11.34 GQDD986 pKa = 2.92 SFTYY990 pKa = 10.01 RR991 pKa = 11.84 ANDD994 pKa = 3.19 GKK996 pKa = 10.8 LYY998 pKa = 11.05 SNIATVTITVNSTTAPNTAPVAVDD1022 pKa = 3.08 NTYY1025 pKa = 10.91 SIDD1028 pKa = 3.63 QNEE1031 pKa = 4.76 VLTIPAPGILQNDD1044 pKa = 3.79 TDD1046 pKa = 4.37 SEE1048 pKa = 4.33 EE1049 pKa = 4.91 DD1050 pKa = 3.53 NLTASLDD1057 pKa = 3.74 SDD1059 pKa = 4.02 VSNGTLTLNADD1070 pKa = 3.4 GSFTYY1075 pKa = 10.51 EE1076 pKa = 3.87 PDD1078 pKa = 3.54 LGYY1081 pKa = 9.36 TGQDD1085 pKa = 2.86 SFTYY1089 pKa = 8.71 TANDD1093 pKa = 3.69 GTDD1096 pKa = 3.54 DD1097 pKa = 4.37 SNVATVTITVNSTTAPNTAPVAEE1120 pKa = 4.31 NDD1122 pKa = 3.82 TYY1124 pKa = 11.67 AVDD1127 pKa = 3.58 QDD1129 pKa = 3.75 EE1130 pKa = 4.79 VLPISSPGILQNDD1143 pKa = 3.43 TDD1145 pKa = 4.47 ADD1147 pKa = 4.12 GDD1149 pKa = 4.01 NLTASLVSDD1158 pKa = 3.92 VSNGTLILNADD1169 pKa = 3.64 GSFTYY1174 pKa = 9.84 TPNSGYY1180 pKa = 10.62 VGQDD1184 pKa = 2.61 SFTYY1188 pKa = 8.73 TANDD1192 pKa = 3.69 GTDD1195 pKa = 3.54 DD1196 pKa = 4.37 SNVATVTITVNSTTAPNTAPVAEE1219 pKa = 4.31 NDD1221 pKa = 3.82 TYY1223 pKa = 11.67 AVDD1226 pKa = 3.58 QDD1228 pKa = 3.75 EE1229 pKa = 4.79 VLPISSPGILQNDD1242 pKa = 3.8 TDD1244 pKa = 4.4 SEE1246 pKa = 4.5 GDD1248 pKa = 3.46 NLTASLVSDD1257 pKa = 4.02 VFNGTLTFNADD1268 pKa = 2.56 GSFIYY1273 pKa = 10.34 EE1274 pKa = 4.09 PSSGFTGQDD1283 pKa = 2.78 SFTYY1287 pKa = 10.04 RR1288 pKa = 11.84 ANDD1291 pKa = 3.35 GKK1293 pKa = 10.88 LDD1295 pKa = 4.06 SNIATVTITVTKK1307 pKa = 9.91 NDD1309 pKa = 3.7 PAAPEE1314 pKa = 4.27 INCLNHH1320 pKa = 7.08 VIFLDD1325 pKa = 3.86 KK1326 pKa = 10.87 NGKK1329 pKa = 9.95 ASLEE1333 pKa = 4.01 PKK1335 pKa = 9.58 TIYY1338 pKa = 10.85 NGDD1341 pKa = 3.69 RR1342 pKa = 11.84 ADD1344 pKa = 4.78 LEE1346 pKa = 4.82 LKK1348 pKa = 10.7 LSQTNFDD1355 pKa = 4.25 CSNLGSNTVTLIATDD1370 pKa = 4.16 PEE1372 pKa = 4.55 TGLSSLCTAHH1382 pKa = 7.97 IEE1384 pKa = 4.15 VLDD1387 pKa = 4.36 VISPEE1392 pKa = 3.85 ASCISGYY1399 pKa = 8.59 VLEE1402 pKa = 5.07 LGEE1405 pKa = 5.77 DD1406 pKa = 3.96 GTASLAAAAIDD1417 pKa = 4.02 NNSTDD1422 pKa = 2.93 NCSIVSRR1429 pKa = 11.84 SVSRR1433 pKa = 11.84 QNFTRR1438 pKa = 11.84 DD1439 pKa = 3.54 DD1440 pKa = 3.64 IGDD1443 pKa = 3.44 VSVRR1447 pKa = 11.84 LTVRR1451 pKa = 11.84 DD1452 pKa = 3.29 AAGNEE1457 pKa = 4.55 GSCEE1461 pKa = 4.04 SVIKK1465 pKa = 11.04 VIDD1468 pKa = 3.64 KK1469 pKa = 8.05 QTSDD1473 pKa = 3.81 FQCKK1477 pKa = 9.17 EE1478 pKa = 4.01 KK1479 pKa = 11.19 VSVSLNEE1486 pKa = 4.1 NGNATLAVEE1495 pKa = 4.17 EE1496 pKa = 5.69 LYY1498 pKa = 10.78 TGDD1501 pKa = 3.37 ASGINFDD1508 pKa = 3.83 KK1509 pKa = 11.22 NNYY1512 pKa = 8.64 QFSCADD1518 pKa = 4.12 LGTKK1522 pKa = 8.9 TIQFNYY1528 pKa = 9.41 SGRR1531 pKa = 11.84 IEE1533 pKa = 4.36 GSCSIKK1539 pKa = 10.8 VEE1541 pKa = 4.43 VKK1543 pKa = 10.89 DD1544 pKa = 4.18 EE1545 pKa = 4.07 LAPTINTNLINASLDD1560 pKa = 3.44 EE1561 pKa = 5.42 DD1562 pKa = 4.05 GFFYY1566 pKa = 10.93 LDD1568 pKa = 3.53 EE1569 pKa = 4.51 NRR1571 pKa = 11.84 VEE1573 pKa = 5.95 AEE1575 pKa = 4.36 DD1576 pKa = 3.65 NCNTPLQITLEE1587 pKa = 4.24 KK1588 pKa = 10.3 TVFTCEE1594 pKa = 4.65 DD1595 pKa = 3.2 VGQNSILVEE1604 pKa = 4.2 AEE1606 pKa = 3.83 DD1607 pKa = 3.76 ASGIKK1612 pKa = 8.92 TSKK1615 pKa = 9.92 EE1616 pKa = 3.21 ITVIVSGAPCEE1627 pKa = 4.01 PSIEE1631 pKa = 4.23 FNDD1634 pKa = 3.48 IRR1636 pKa = 11.84 ISPNPSAGIFKK1647 pKa = 10.37 IGTPTDD1653 pKa = 3.1 IKK1655 pKa = 10.66 INEE1658 pKa = 4.17 VKK1660 pKa = 10.88 VFDD1663 pKa = 3.51 SRR1665 pKa = 11.84 GRR1667 pKa = 11.84 FITQKK1672 pKa = 10.35 DD1673 pKa = 3.74 YY1674 pKa = 11.84 NMNFRR1679 pKa = 11.84 FYY1681 pKa = 11.22 KK1682 pKa = 10.74 LNLEE1686 pKa = 4.24 SLQEE1690 pKa = 4.02 SVYY1693 pKa = 10.22 TLQIFTNNGEE1703 pKa = 4.07 FVKK1706 pKa = 10.7 RR1707 pKa = 11.84 IIIKK1711 pKa = 10.21 RR1712 pKa = 3.59
Molecular weight: 186.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.706
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.884
Patrickios 0.935
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A1M5C4P9|A0A1M5C4P9_9FLAO Probable transaldolase OS=Salegentibacter echinorum OX=1073325 GN=tal PE=3 SV=1
MM1 pKa = 7.12 FQFDD5 pKa = 3.71 QEE7 pKa = 4.27 TWVYY11 pKa = 10.11 IILIAVFLAYY21 pKa = 9.27 FIWNSRR27 pKa = 11.84 RR28 pKa = 11.84 AKK30 pKa = 10.25 KK31 pKa = 9.86 NRR33 pKa = 11.84 KK34 pKa = 8.15 ARR36 pKa = 11.84 KK37 pKa = 8.53 NRR39 pKa = 11.84 NFRR42 pKa = 11.84 KK43 pKa = 9.61 RR44 pKa = 11.84 YY45 pKa = 6.27 MEE47 pKa = 4.0 RR48 pKa = 11.84 RR49 pKa = 11.84 EE50 pKa = 4.11 EE51 pKa = 3.91 QDD53 pKa = 2.89 KK54 pKa = 9.47 TT55 pKa = 3.68
Molecular weight: 7.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.911
IPC_protein 10.789
Toseland 10.921
ProMoST 10.657
Dawson 10.994
Bjellqvist 10.716
Wikipedia 11.228
Rodwell 11.228
Grimsley 11.038
Solomon 11.14
Lehninger 11.111
Nozaki 10.877
DTASelect 10.716
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.921
Patrickios 10.979
IPC_peptide 11.155
IPC2_peptide 9.56
IPC2.peptide.svr19 8.452
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3479
0
3479
1135832
29
2388
326.5
36.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.598 ± 0.034
0.665 ± 0.012
5.459 ± 0.034
7.459 ± 0.042
5.237 ± 0.03
6.415 ± 0.039
1.766 ± 0.019
7.579 ± 0.039
8.208 ± 0.05
9.466 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.021
5.895 ± 0.039
3.464 ± 0.021
3.422 ± 0.025
3.812 ± 0.025
6.229 ± 0.034
5.151 ± 0.029
5.909 ± 0.03
1.073 ± 0.014
3.976 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here