Lewinellaceae bacterium SD302

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Lewinellaceae; unclassified Lewinellaceae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2C6CQR1|A0A2C6CQR1_9BACT Uncharacterized protein OS=Lewinellaceae bacterium SD302 OX=2014804 GN=CEQ90_07730 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TLTTFLFFLAALNFIAGQSFRR23 pKa = 11.84TTLVSPATVSRR34 pKa = 11.84QTSNSDD40 pKa = 3.45LNGDD44 pKa = 3.94GHH46 pKa = 7.0QDD48 pKa = 4.08LITVNGEE55 pKa = 4.0VGWLKK60 pKa = 10.32WVVGAHH66 pKa = 7.41AYY68 pKa = 9.6PEE70 pKa = 4.14YY71 pKa = 10.89RR72 pKa = 11.84KK73 pKa = 8.39ITPDD77 pKa = 2.99EE78 pKa = 4.58NYY80 pKa = 8.93THH82 pKa = 7.45ALTGDD87 pKa = 3.33VDD89 pKa = 4.15SDD91 pKa = 3.77GDD93 pKa = 3.6IDD95 pKa = 4.92IVALGDD101 pKa = 3.73DD102 pKa = 3.63RR103 pKa = 11.84VVLLKK108 pKa = 11.28NNDD111 pKa = 3.28GEE113 pKa = 4.36AGFWLEE119 pKa = 5.07SMIDD123 pKa = 3.72DD124 pKa = 4.9EE125 pKa = 5.35EE126 pKa = 5.16VNDD129 pKa = 4.85PDD131 pKa = 3.5WGALGDD137 pKa = 3.74IDD139 pKa = 5.83GDD141 pKa = 3.91GDD143 pKa = 4.68LDD145 pKa = 4.24LLVASRR151 pKa = 11.84YY152 pKa = 9.87GYY154 pKa = 10.31YY155 pKa = 10.41LGWYY159 pKa = 7.79EE160 pKa = 4.11NTDD163 pKa = 3.24GTGEE167 pKa = 4.0FQFYY171 pKa = 11.32AEE173 pKa = 5.63LPDD176 pKa = 3.88AQNARR181 pKa = 11.84SFGALVDD188 pKa = 4.38FDD190 pKa = 4.04QDD192 pKa = 2.76GDD194 pKa = 4.45LDD196 pKa = 4.04VLSAGCYY203 pKa = 9.8LDD205 pKa = 5.5CTLEE209 pKa = 3.92WYY211 pKa = 9.0EE212 pKa = 3.99NLDD215 pKa = 3.55GEE217 pKa = 5.01GEE219 pKa = 3.95FGIRR223 pKa = 11.84KK224 pKa = 9.39EE225 pKa = 4.7IYY227 pKa = 9.23FDD229 pKa = 4.33DD230 pKa = 4.25YY231 pKa = 11.48GIEE234 pKa = 4.02NMVIDD239 pKa = 4.31VGDD242 pKa = 3.56VDD244 pKa = 3.89GDD246 pKa = 4.0GYY248 pKa = 11.02EE249 pKa = 5.63DD250 pKa = 4.12IMTTSTYY257 pKa = 10.17YY258 pKa = 10.7KK259 pKa = 9.6VIRR262 pKa = 11.84WHH264 pKa = 6.71RR265 pKa = 11.84NLIGSGEE272 pKa = 4.17MGFAAAVDD280 pKa = 3.36ISDD283 pKa = 4.19FDD285 pKa = 3.62WARR288 pKa = 11.84SIEE291 pKa = 4.38LTDD294 pKa = 4.74LDD296 pKa = 4.61NDD298 pKa = 3.48NDD300 pKa = 3.96LDD302 pKa = 3.81IAVAGDD308 pKa = 4.5DD309 pKa = 3.89DD310 pKa = 3.9THH312 pKa = 8.55LIGWYY317 pKa = 9.21EE318 pKa = 3.92NWSGDD323 pKa = 4.0GLFSNLNIIDD333 pKa = 4.78DD334 pKa = 4.31EE335 pKa = 4.49TDD337 pKa = 2.62VDD339 pKa = 4.29YY340 pKa = 11.71SRR342 pKa = 11.84LICFDD347 pKa = 2.99IDD349 pKa = 3.69EE350 pKa = 5.36NGTQDD355 pKa = 3.31ILTAGTLNSHH365 pKa = 6.57TIGWYY370 pKa = 10.37ANEE373 pKa = 4.25EE374 pKa = 4.18NTGSFGRR381 pKa = 11.84VNEE384 pKa = 4.1LVHH387 pKa = 6.75PYY389 pKa = 10.04IADD392 pKa = 4.05EE393 pKa = 4.25IMLNDD398 pKa = 3.28VDD400 pKa = 5.41GDD402 pKa = 4.17GNTDD406 pKa = 2.95IVAGGSWYY414 pKa = 9.75TNSGYY419 pKa = 10.93DD420 pKa = 3.33YY421 pKa = 11.09LAFSNIRR428 pKa = 11.84MVDD431 pKa = 3.66EE432 pKa = 4.91EE433 pKa = 4.55GHH435 pKa = 6.29LSLVEE440 pKa = 4.9DD441 pKa = 4.44FTNDD445 pKa = 3.94GITDD449 pKa = 5.94LITLEE454 pKa = 4.16RR455 pKa = 11.84LPLSTHH461 pKa = 5.78KK462 pKa = 10.79KK463 pKa = 7.45IVLYY467 pKa = 10.17EE468 pKa = 3.84RR469 pKa = 11.84SGEE472 pKa = 4.0QFIFSSDD479 pKa = 2.77IVEE482 pKa = 4.15VDD484 pKa = 2.97NASIKK489 pKa = 10.88NLVSLDD495 pKa = 3.56YY496 pKa = 11.56DD497 pKa = 3.56NDD499 pKa = 3.75GDD501 pKa = 5.17LDD503 pKa = 3.96FVYY506 pKa = 9.05QTAYY510 pKa = 10.8DD511 pKa = 4.14VVWLCEE517 pKa = 4.0NLGSDD522 pKa = 3.77NGFAAPVEE530 pKa = 4.24IADD533 pKa = 4.24TEE535 pKa = 4.38VSVNNFEE542 pKa = 5.91LGDD545 pKa = 4.1LDD547 pKa = 3.78GDD549 pKa = 4.31GYY551 pKa = 11.74VDD553 pKa = 4.43ILIGTDD559 pKa = 2.53WNQRR563 pKa = 11.84MGWYY567 pKa = 9.96RR568 pKa = 11.84NEE570 pKa = 4.27AGSGTFSDD578 pKa = 4.39LAIISSGQPYY588 pKa = 9.68EE589 pKa = 3.95QLDD592 pKa = 3.51WDD594 pKa = 4.49DD595 pKa = 3.77QDD597 pKa = 5.26GDD599 pKa = 3.78GDD601 pKa = 4.46LDD603 pKa = 3.75LVYY606 pKa = 10.71VYY608 pKa = 10.6EE609 pKa = 4.19EE610 pKa = 4.28NILRR614 pKa = 11.84RR615 pKa = 11.84NNEE618 pKa = 3.7GNGVFSSEE626 pKa = 3.79EE627 pKa = 4.01VVYY630 pKa = 10.63HH631 pKa = 6.84DD632 pKa = 4.19PAGGHH637 pKa = 4.9SFRR640 pKa = 11.84RR641 pKa = 11.84IYY643 pKa = 11.16CNDD646 pKa = 3.35HH647 pKa = 6.74NDD649 pKa = 3.43DD650 pKa = 3.82GLRR653 pKa = 11.84DD654 pKa = 3.83FLFFDD659 pKa = 3.36QSSYY663 pKa = 9.4GWRR666 pKa = 11.84FLTAEE671 pKa = 3.97IEE673 pKa = 4.08GGYY676 pKa = 10.12LLSAEE681 pKa = 4.6AEE683 pKa = 4.52FIPSNNVEE691 pKa = 4.37PIKK694 pKa = 10.72FYY696 pKa = 11.3DD697 pKa = 4.24LDD699 pKa = 4.09NDD701 pKa = 4.32GDD703 pKa = 4.43DD704 pKa = 5.75DD705 pKa = 4.09MLFAQGDD712 pKa = 3.8KK713 pKa = 11.03LYY715 pKa = 11.17VSEE718 pKa = 4.65NLNSGKK724 pKa = 9.89VLQGLIYY731 pKa = 10.51YY732 pKa = 9.99DD733 pKa = 3.86EE734 pKa = 4.77NEE736 pKa = 4.11NGINDD741 pKa = 4.35DD742 pKa = 3.78SDD744 pKa = 4.24SPLVDD749 pKa = 3.17QLVSIDD755 pKa = 3.53PTQLFLHH762 pKa = 6.72SNTDD766 pKa = 3.5GLFSTVLGNGQYY778 pKa = 9.22EE779 pKa = 4.58VRR781 pKa = 11.84CDD783 pKa = 4.38PEE785 pKa = 5.13DD786 pKa = 3.16GWGFVGDD793 pKa = 4.54SIVNIQTSSDD803 pKa = 3.28ADD805 pKa = 4.31TIWVEE810 pKa = 4.24FPLINTDD817 pKa = 3.17TTSRR821 pKa = 11.84PSVEE825 pKa = 4.26VTSGPVRR832 pKa = 11.84CGFEE836 pKa = 4.09VPFWLTVNNDD846 pKa = 2.99GYY848 pKa = 11.59VPEE851 pKa = 4.57TGDD854 pKa = 3.34FYY856 pKa = 11.33FYY858 pKa = 10.97LDD860 pKa = 3.99SLVALAIDD868 pKa = 4.42PADD871 pKa = 3.95HH872 pKa = 7.18PNLFVDD878 pKa = 3.82AEE880 pKa = 4.31GRR882 pKa = 11.84TGLFWEE888 pKa = 5.12QIDD891 pKa = 3.78ISPGARR897 pKa = 11.84WQTQVLLQMPSVDD910 pKa = 5.05FIGEE914 pKa = 3.84ALGFNGIFEE923 pKa = 4.49YY924 pKa = 10.86AVGINEE930 pKa = 4.03LQSNGEE936 pKa = 3.88YY937 pKa = 10.27TRR939 pKa = 11.84VLNCSYY945 pKa = 11.14DD946 pKa = 3.75PNDD949 pKa = 3.54KK950 pKa = 10.75SVEE953 pKa = 4.16EE954 pKa = 4.42NLPGYY959 pKa = 9.16PGYY962 pKa = 11.73SMVGDD967 pKa = 3.65SLEE970 pKa = 3.89YY971 pKa = 10.62LIRR974 pKa = 11.84FQNLGSDD981 pKa = 3.33TAINIKK987 pKa = 10.46VSDD990 pKa = 3.8QLSEE994 pKa = 3.95HH995 pKa = 6.74FDD997 pKa = 3.39RR998 pKa = 11.84SKK1000 pKa = 11.32LRR1002 pKa = 11.84ILSASHH1008 pKa = 6.94NYY1010 pKa = 10.07RR1011 pKa = 11.84MVLTDD1016 pKa = 4.08DD1017 pKa = 3.66NRR1019 pKa = 11.84LNVYY1023 pKa = 9.93FDD1025 pKa = 4.5NIYY1028 pKa = 10.86LPDD1031 pKa = 3.99AMTDD1035 pKa = 3.29EE1036 pKa = 5.05AGSHH1040 pKa = 5.74GFIKK1044 pKa = 10.39FRR1046 pKa = 11.84VGMLPGSTDD1055 pKa = 2.85GDD1057 pKa = 3.96VIEE1060 pKa = 4.8NQADD1064 pKa = 3.42IYY1066 pKa = 11.19FDD1068 pKa = 4.28FNPPITTNVVYY1079 pKa = 8.95NTILEE1084 pKa = 4.14RR1085 pKa = 11.84LIYY1088 pKa = 8.85PAQILAPKK1096 pKa = 9.98CFEE1099 pKa = 4.35FSDD1102 pKa = 4.25GSISVEE1108 pKa = 3.81EE1109 pKa = 4.21VVNGVDD1115 pKa = 3.84YY1116 pKa = 10.13FWNGISSSNHH1126 pKa = 5.54IEE1128 pKa = 4.02NLSAGTNTLEE1138 pKa = 4.7VYY1140 pKa = 10.56RR1141 pKa = 11.84SDD1143 pKa = 4.07GKK1145 pKa = 10.7ILADD1149 pKa = 3.4TSYY1152 pKa = 11.14QITDD1156 pKa = 3.58PDD1158 pKa = 3.64ILEE1161 pKa = 4.06VTAEE1165 pKa = 3.97IEE1167 pKa = 4.05PASFANSGSIIATPTGGTAPYY1188 pKa = 9.4TYY1190 pKa = 10.29VWQQFPEE1197 pKa = 4.07QQGNEE1202 pKa = 4.09IGDD1205 pKa = 4.08LAAGNYY1211 pKa = 9.09DD1212 pKa = 4.01LQVIDD1217 pKa = 5.18ANGCIIDD1224 pKa = 3.86AVFIVPEE1231 pKa = 4.21VVSTAEE1237 pKa = 4.25HH1238 pKa = 6.47LAKK1241 pKa = 10.66SPLSLRR1247 pKa = 11.84IYY1249 pKa = 9.3PNPARR1254 pKa = 11.84ALEE1257 pKa = 4.05FVNIRR1262 pKa = 11.84FSEE1265 pKa = 4.29VKK1267 pKa = 10.55NEE1269 pKa = 4.07SEE1271 pKa = 4.38LLVYY1275 pKa = 10.83DD1276 pKa = 3.47NLKK1279 pKa = 10.2RR1280 pKa = 11.84IVVRR1284 pKa = 11.84RR1285 pKa = 11.84SIAPGSYY1292 pKa = 10.29EE1293 pKa = 4.13LTDD1296 pKa = 4.11LKK1298 pKa = 10.69LAPGNYY1304 pKa = 9.27FLVLISGGKK1313 pKa = 8.11HH1314 pKa = 4.32ALGRR1318 pKa = 11.84LVIIGSS1324 pKa = 3.66

Molecular weight:
147.2 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2C6BUT0|A0A2C6BUT0_9BACT Uncharacterized protein OS=Lewinellaceae bacterium SD302 OX=2014804 GN=CEQ90_19755 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84HH3 pKa = 5.52RR4 pKa = 11.84QLPADD9 pKa = 4.01TSAASVTYY17 pKa = 9.85RR18 pKa = 11.84RR19 pKa = 11.84HH20 pKa = 4.45QFRR23 pKa = 11.84IFDD26 pKa = 4.01PSRR29 pKa = 11.84LKK31 pKa = 10.57IGSCVGAPVLYY42 pKa = 7.97NTPGKK47 pKa = 10.23NGQLPISEE55 pKa = 4.23IAIMILL61 pKa = 3.33

Molecular weight:
6.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4052

0

4052

1596819

38

4969

394.1

43.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.856 ± 0.042

0.985 ± 0.027

6.346 ± 0.045

6.531 ± 0.039

4.713 ± 0.029

7.698 ± 0.037

1.717 ± 0.022

6.041 ± 0.03

4.233 ± 0.053

10.026 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.02

4.875 ± 0.038

4.42 ± 0.03

3.661 ± 0.023

5.235 ± 0.05

6.207 ± 0.03

5.904 ± 0.063

6.545 ± 0.031

1.221 ± 0.017

3.758 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski