Lewinellaceae bacterium SD302
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4052 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2C6CQR1|A0A2C6CQR1_9BACT Uncharacterized protein OS=Lewinellaceae bacterium SD302 OX=2014804 GN=CEQ90_07730 PE=4 SV=1
MM1 pKa = 7.38 RR2 pKa = 11.84 TLTTFLFFLAALNFIAGQSFRR23 pKa = 11.84 TTLVSPATVSRR34 pKa = 11.84 QTSNSDD40 pKa = 3.45 LNGDD44 pKa = 3.94 GHH46 pKa = 7.0 QDD48 pKa = 4.08 LITVNGEE55 pKa = 4.0 VGWLKK60 pKa = 10.32 WVVGAHH66 pKa = 7.41 AYY68 pKa = 9.6 PEE70 pKa = 4.14 YY71 pKa = 10.89 RR72 pKa = 11.84 KK73 pKa = 8.39 ITPDD77 pKa = 2.99 EE78 pKa = 4.58 NYY80 pKa = 8.93 THH82 pKa = 7.45 ALTGDD87 pKa = 3.33 VDD89 pKa = 4.15 SDD91 pKa = 3.77 GDD93 pKa = 3.6 IDD95 pKa = 4.92 IVALGDD101 pKa = 3.73 DD102 pKa = 3.63 RR103 pKa = 11.84 VVLLKK108 pKa = 11.28 NNDD111 pKa = 3.28 GEE113 pKa = 4.36 AGFWLEE119 pKa = 5.07 SMIDD123 pKa = 3.72 DD124 pKa = 4.9 EE125 pKa = 5.35 EE126 pKa = 5.16 VNDD129 pKa = 4.85 PDD131 pKa = 3.5 WGALGDD137 pKa = 3.74 IDD139 pKa = 5.83 GDD141 pKa = 3.91 GDD143 pKa = 4.68 LDD145 pKa = 4.24 LLVASRR151 pKa = 11.84 YY152 pKa = 9.87 GYY154 pKa = 10.31 YY155 pKa = 10.41 LGWYY159 pKa = 7.79 EE160 pKa = 4.11 NTDD163 pKa = 3.24 GTGEE167 pKa = 4.0 FQFYY171 pKa = 11.32 AEE173 pKa = 5.63 LPDD176 pKa = 3.88 AQNARR181 pKa = 11.84 SFGALVDD188 pKa = 4.38 FDD190 pKa = 4.04 QDD192 pKa = 2.76 GDD194 pKa = 4.45 LDD196 pKa = 4.04 VLSAGCYY203 pKa = 9.8 LDD205 pKa = 5.5 CTLEE209 pKa = 3.92 WYY211 pKa = 9.0 EE212 pKa = 3.99 NLDD215 pKa = 3.55 GEE217 pKa = 5.01 GEE219 pKa = 3.95 FGIRR223 pKa = 11.84 KK224 pKa = 9.39 EE225 pKa = 4.7 IYY227 pKa = 9.23 FDD229 pKa = 4.33 DD230 pKa = 4.25 YY231 pKa = 11.48 GIEE234 pKa = 4.02 NMVIDD239 pKa = 4.31 VGDD242 pKa = 3.56 VDD244 pKa = 3.89 GDD246 pKa = 4.0 GYY248 pKa = 11.02 EE249 pKa = 5.63 DD250 pKa = 4.12 IMTTSTYY257 pKa = 10.17 YY258 pKa = 10.7 KK259 pKa = 9.6 VIRR262 pKa = 11.84 WHH264 pKa = 6.71 RR265 pKa = 11.84 NLIGSGEE272 pKa = 4.17 MGFAAAVDD280 pKa = 3.36 ISDD283 pKa = 4.19 FDD285 pKa = 3.62 WARR288 pKa = 11.84 SIEE291 pKa = 4.38 LTDD294 pKa = 4.74 LDD296 pKa = 4.61 NDD298 pKa = 3.48 NDD300 pKa = 3.96 LDD302 pKa = 3.81 IAVAGDD308 pKa = 4.5 DD309 pKa = 3.89 DD310 pKa = 3.9 THH312 pKa = 8.55 LIGWYY317 pKa = 9.21 EE318 pKa = 3.92 NWSGDD323 pKa = 4.0 GLFSNLNIIDD333 pKa = 4.78 DD334 pKa = 4.31 EE335 pKa = 4.49 TDD337 pKa = 2.62 VDD339 pKa = 4.29 YY340 pKa = 11.71 SRR342 pKa = 11.84 LICFDD347 pKa = 2.99 IDD349 pKa = 3.69 EE350 pKa = 5.36 NGTQDD355 pKa = 3.31 ILTAGTLNSHH365 pKa = 6.57 TIGWYY370 pKa = 10.37 ANEE373 pKa = 4.25 EE374 pKa = 4.18 NTGSFGRR381 pKa = 11.84 VNEE384 pKa = 4.1 LVHH387 pKa = 6.75 PYY389 pKa = 10.04 IADD392 pKa = 4.05 EE393 pKa = 4.25 IMLNDD398 pKa = 3.28 VDD400 pKa = 5.41 GDD402 pKa = 4.17 GNTDD406 pKa = 2.95 IVAGGSWYY414 pKa = 9.75 TNSGYY419 pKa = 10.93 DD420 pKa = 3.33 YY421 pKa = 11.09 LAFSNIRR428 pKa = 11.84 MVDD431 pKa = 3.66 EE432 pKa = 4.91 EE433 pKa = 4.55 GHH435 pKa = 6.29 LSLVEE440 pKa = 4.9 DD441 pKa = 4.44 FTNDD445 pKa = 3.94 GITDD449 pKa = 5.94 LITLEE454 pKa = 4.16 RR455 pKa = 11.84 LPLSTHH461 pKa = 5.78 KK462 pKa = 10.79 KK463 pKa = 7.45 IVLYY467 pKa = 10.17 EE468 pKa = 3.84 RR469 pKa = 11.84 SGEE472 pKa = 4.0 QFIFSSDD479 pKa = 2.77 IVEE482 pKa = 4.15 VDD484 pKa = 2.97 NASIKK489 pKa = 10.88 NLVSLDD495 pKa = 3.56 YY496 pKa = 11.56 DD497 pKa = 3.56 NDD499 pKa = 3.75 GDD501 pKa = 5.17 LDD503 pKa = 3.96 FVYY506 pKa = 9.05 QTAYY510 pKa = 10.8 DD511 pKa = 4.14 VVWLCEE517 pKa = 4.0 NLGSDD522 pKa = 3.77 NGFAAPVEE530 pKa = 4.24 IADD533 pKa = 4.24 TEE535 pKa = 4.38 VSVNNFEE542 pKa = 5.91 LGDD545 pKa = 4.1 LDD547 pKa = 3.78 GDD549 pKa = 4.31 GYY551 pKa = 11.74 VDD553 pKa = 4.43 ILIGTDD559 pKa = 2.53 WNQRR563 pKa = 11.84 MGWYY567 pKa = 9.96 RR568 pKa = 11.84 NEE570 pKa = 4.27 AGSGTFSDD578 pKa = 4.39 LAIISSGQPYY588 pKa = 9.68 EE589 pKa = 3.95 QLDD592 pKa = 3.51 WDD594 pKa = 4.49 DD595 pKa = 3.77 QDD597 pKa = 5.26 GDD599 pKa = 3.78 GDD601 pKa = 4.46 LDD603 pKa = 3.75 LVYY606 pKa = 10.71 VYY608 pKa = 10.6 EE609 pKa = 4.19 EE610 pKa = 4.28 NILRR614 pKa = 11.84 RR615 pKa = 11.84 NNEE618 pKa = 3.7 GNGVFSSEE626 pKa = 3.79 EE627 pKa = 4.01 VVYY630 pKa = 10.63 HH631 pKa = 6.84 DD632 pKa = 4.19 PAGGHH637 pKa = 4.9 SFRR640 pKa = 11.84 RR641 pKa = 11.84 IYY643 pKa = 11.16 CNDD646 pKa = 3.35 HH647 pKa = 6.74 NDD649 pKa = 3.43 DD650 pKa = 3.82 GLRR653 pKa = 11.84 DD654 pKa = 3.83 FLFFDD659 pKa = 3.36 QSSYY663 pKa = 9.4 GWRR666 pKa = 11.84 FLTAEE671 pKa = 3.97 IEE673 pKa = 4.08 GGYY676 pKa = 10.12 LLSAEE681 pKa = 4.6 AEE683 pKa = 4.52 FIPSNNVEE691 pKa = 4.37 PIKK694 pKa = 10.72 FYY696 pKa = 11.3 DD697 pKa = 4.24 LDD699 pKa = 4.09 NDD701 pKa = 4.32 GDD703 pKa = 4.43 DD704 pKa = 5.75 DD705 pKa = 4.09 MLFAQGDD712 pKa = 3.8 KK713 pKa = 11.03 LYY715 pKa = 11.17 VSEE718 pKa = 4.65 NLNSGKK724 pKa = 9.89 VLQGLIYY731 pKa = 10.51 YY732 pKa = 9.99 DD733 pKa = 3.86 EE734 pKa = 4.77 NEE736 pKa = 4.11 NGINDD741 pKa = 4.35 DD742 pKa = 3.78 SDD744 pKa = 4.24 SPLVDD749 pKa = 3.17 QLVSIDD755 pKa = 3.53 PTQLFLHH762 pKa = 6.72 SNTDD766 pKa = 3.5 GLFSTVLGNGQYY778 pKa = 9.22 EE779 pKa = 4.58 VRR781 pKa = 11.84 CDD783 pKa = 4.38 PEE785 pKa = 5.13 DD786 pKa = 3.16 GWGFVGDD793 pKa = 4.54 SIVNIQTSSDD803 pKa = 3.28 ADD805 pKa = 4.31 TIWVEE810 pKa = 4.24 FPLINTDD817 pKa = 3.17 TTSRR821 pKa = 11.84 PSVEE825 pKa = 4.26 VTSGPVRR832 pKa = 11.84 CGFEE836 pKa = 4.09 VPFWLTVNNDD846 pKa = 2.99 GYY848 pKa = 11.59 VPEE851 pKa = 4.57 TGDD854 pKa = 3.34 FYY856 pKa = 11.33 FYY858 pKa = 10.97 LDD860 pKa = 3.99 SLVALAIDD868 pKa = 4.42 PADD871 pKa = 3.95 HH872 pKa = 7.18 PNLFVDD878 pKa = 3.82 AEE880 pKa = 4.31 GRR882 pKa = 11.84 TGLFWEE888 pKa = 5.12 QIDD891 pKa = 3.78 ISPGARR897 pKa = 11.84 WQTQVLLQMPSVDD910 pKa = 5.05 FIGEE914 pKa = 3.84 ALGFNGIFEE923 pKa = 4.49 YY924 pKa = 10.86 AVGINEE930 pKa = 4.03 LQSNGEE936 pKa = 3.88 YY937 pKa = 10.27 TRR939 pKa = 11.84 VLNCSYY945 pKa = 11.14 DD946 pKa = 3.75 PNDD949 pKa = 3.54 KK950 pKa = 10.75 SVEE953 pKa = 4.16 EE954 pKa = 4.42 NLPGYY959 pKa = 9.16 PGYY962 pKa = 11.73 SMVGDD967 pKa = 3.65 SLEE970 pKa = 3.89 YY971 pKa = 10.62 LIRR974 pKa = 11.84 FQNLGSDD981 pKa = 3.33 TAINIKK987 pKa = 10.46 VSDD990 pKa = 3.8 QLSEE994 pKa = 3.95 HH995 pKa = 6.74 FDD997 pKa = 3.39 RR998 pKa = 11.84 SKK1000 pKa = 11.32 LRR1002 pKa = 11.84 ILSASHH1008 pKa = 6.94 NYY1010 pKa = 10.07 RR1011 pKa = 11.84 MVLTDD1016 pKa = 4.08 DD1017 pKa = 3.66 NRR1019 pKa = 11.84 LNVYY1023 pKa = 9.93 FDD1025 pKa = 4.5 NIYY1028 pKa = 10.86 LPDD1031 pKa = 3.99 AMTDD1035 pKa = 3.29 EE1036 pKa = 5.05 AGSHH1040 pKa = 5.74 GFIKK1044 pKa = 10.39 FRR1046 pKa = 11.84 VGMLPGSTDD1055 pKa = 2.85 GDD1057 pKa = 3.96 VIEE1060 pKa = 4.8 NQADD1064 pKa = 3.42 IYY1066 pKa = 11.19 FDD1068 pKa = 4.28 FNPPITTNVVYY1079 pKa = 8.95 NTILEE1084 pKa = 4.14 RR1085 pKa = 11.84 LIYY1088 pKa = 8.85 PAQILAPKK1096 pKa = 9.98 CFEE1099 pKa = 4.35 FSDD1102 pKa = 4.25 GSISVEE1108 pKa = 3.81 EE1109 pKa = 4.21 VVNGVDD1115 pKa = 3.84 YY1116 pKa = 10.13 FWNGISSSNHH1126 pKa = 5.54 IEE1128 pKa = 4.02 NLSAGTNTLEE1138 pKa = 4.7 VYY1140 pKa = 10.56 RR1141 pKa = 11.84 SDD1143 pKa = 4.07 GKK1145 pKa = 10.7 ILADD1149 pKa = 3.4 TSYY1152 pKa = 11.14 QITDD1156 pKa = 3.58 PDD1158 pKa = 3.64 ILEE1161 pKa = 4.06 VTAEE1165 pKa = 3.97 IEE1167 pKa = 4.05 PASFANSGSIIATPTGGTAPYY1188 pKa = 9.4 TYY1190 pKa = 10.29 VWQQFPEE1197 pKa = 4.07 QQGNEE1202 pKa = 4.09 IGDD1205 pKa = 4.08 LAAGNYY1211 pKa = 9.09 DD1212 pKa = 4.01 LQVIDD1217 pKa = 5.18 ANGCIIDD1224 pKa = 3.86 AVFIVPEE1231 pKa = 4.21 VVSTAEE1237 pKa = 4.25 HH1238 pKa = 6.47 LAKK1241 pKa = 10.66 SPLSLRR1247 pKa = 11.84 IYY1249 pKa = 9.3 PNPARR1254 pKa = 11.84 ALEE1257 pKa = 4.05 FVNIRR1262 pKa = 11.84 FSEE1265 pKa = 4.29 VKK1267 pKa = 10.55 NEE1269 pKa = 4.07 SEE1271 pKa = 4.38 LLVYY1275 pKa = 10.83 DD1276 pKa = 3.47 NLKK1279 pKa = 10.2 RR1280 pKa = 11.84 IVVRR1284 pKa = 11.84 RR1285 pKa = 11.84 SIAPGSYY1292 pKa = 10.29 EE1293 pKa = 4.13 LTDD1296 pKa = 4.11 LKK1298 pKa = 10.69 LAPGNYY1304 pKa = 9.27 FLVLISGGKK1313 pKa = 8.11 HH1314 pKa = 4.32 ALGRR1318 pKa = 11.84 LVIIGSS1324 pKa = 3.66
Molecular weight: 147.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.579
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.923
Patrickios 1.087
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A2C6BUT0|A0A2C6BUT0_9BACT Uncharacterized protein OS=Lewinellaceae bacterium SD302 OX=2014804 GN=CEQ90_19755 PE=4 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 HH3 pKa = 5.52 RR4 pKa = 11.84 QLPADD9 pKa = 4.01 TSAASVTYY17 pKa = 9.85 RR18 pKa = 11.84 RR19 pKa = 11.84 HH20 pKa = 4.45 QFRR23 pKa = 11.84 IFDD26 pKa = 4.01 PSRR29 pKa = 11.84 LKK31 pKa = 10.57 IGSCVGAPVLYY42 pKa = 7.97 NTPGKK47 pKa = 10.23 NGQLPISEE55 pKa = 4.23 IAIMILL61 pKa = 3.33
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.736
IPC_protein 10.613
Toseland 10.496
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.804
Grimsley 10.73
Solomon 10.774
Lehninger 10.745
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.906
Sillero 10.584
Patrickios 10.657
IPC_peptide 10.774
IPC2_peptide 9.575
IPC2.peptide.svr19 8.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4052
0
4052
1596819
38
4969
394.1
43.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.856 ± 0.042
0.985 ± 0.027
6.346 ± 0.045
6.531 ± 0.039
4.713 ± 0.029
7.698 ± 0.037
1.717 ± 0.022
6.041 ± 0.03
4.233 ± 0.053
10.026 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.027 ± 0.02
4.875 ± 0.038
4.42 ± 0.03
3.661 ± 0.023
5.235 ± 0.05
6.207 ± 0.03
5.904 ± 0.063
6.545 ± 0.031
1.221 ± 0.017
3.758 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here