Rhodobacteraceae bacterium WD3A24

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8ERE2|A0A2S8ERE2_9RHOB Cytochrome c1 OS=Rhodobacteraceae bacterium WD3A24 OX=2086263 GN=C2I36_12535 PE=4 SV=1
MM1 pKa = 8.0KK2 pKa = 10.5YY3 pKa = 9.95PIIAAGVLVAGMAHH17 pKa = 6.25AQSEE21 pKa = 4.71QPTLTVYY28 pKa = 9.88TYY30 pKa = 11.17EE31 pKa = 4.62SFVSDD36 pKa = 3.98WGPGPVIEE44 pKa = 4.94EE45 pKa = 3.91NFEE48 pKa = 4.52AICACDD54 pKa = 3.6LRR56 pKa = 11.84LVGVGDD62 pKa = 3.72GAEE65 pKa = 3.87LLGRR69 pKa = 11.84LRR71 pKa = 11.84LEE73 pKa = 4.69GPRR76 pKa = 11.84TDD78 pKa = 3.49ADD80 pKa = 3.76VVLGLDD86 pKa = 3.35TNLIARR92 pKa = 11.84AEE94 pKa = 4.34GTGLFAPHH102 pKa = 7.12GIEE105 pKa = 4.71APEE108 pKa = 3.79FDD110 pKa = 5.5MPVDD114 pKa = 3.31WDD116 pKa = 3.9NPTFLPYY123 pKa = 10.58DD124 pKa = 3.12WGYY127 pKa = 10.21FAFVYY132 pKa = 10.66DD133 pKa = 3.85SDD135 pKa = 4.71VVFDD139 pKa = 4.21PPEE142 pKa = 4.26SFGEE146 pKa = 4.6LIDD149 pKa = 4.31SDD151 pKa = 4.61LSVVIQDD158 pKa = 4.46PRR160 pKa = 11.84SSTPGLGLLMWVKK173 pKa = 10.36AAYY176 pKa = 10.2GDD178 pKa = 3.96DD179 pKa = 4.23APEE182 pKa = 3.82IWEE185 pKa = 4.21GLAPRR190 pKa = 11.84VLTVTQGWSEE200 pKa = 4.28AYY202 pKa = 10.31GLFTEE207 pKa = 5.91GEE209 pKa = 3.84ADD211 pKa = 3.25MALSYY216 pKa = 7.64TTSPAYY222 pKa = 10.24HH223 pKa = 7.32LIAEE227 pKa = 5.09DD228 pKa = 3.99DD229 pKa = 3.88PSYY232 pKa = 11.01AAAVFEE238 pKa = 4.84EE239 pKa = 4.26GHH241 pKa = 5.37YY242 pKa = 9.51MQVEE246 pKa = 4.25VAGRR250 pKa = 11.84IAASDD255 pKa = 3.73QPEE258 pKa = 4.07LAQEE262 pKa = 4.5FLRR265 pKa = 11.84FMLTDD270 pKa = 4.23GFQGVIPTTNWMYY283 pKa = 10.52PAVTPEE289 pKa = 3.86GGLPEE294 pKa = 4.6GFEE297 pKa = 4.3TLADD301 pKa = 4.41PDD303 pKa = 3.96TALLFSAQEE312 pKa = 3.79AAEE315 pKa = 4.27LRR317 pKa = 11.84DD318 pKa = 3.5AALDD322 pKa = 3.38EE323 pKa = 4.29WLNALSRR330 pKa = 3.92

Molecular weight: 35.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8EW26|A0A2S8EW26_9RHOB Acetolactate synthase OS=Rhodobacteraceae bacterium WD3A24 OX=2086263 GN=C2I36_04165 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.56ILNARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.5RR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.74LSAA44 pKa = 4.0

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3201

0

3201

994201

27

1832

310.6

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.878 ± 0.062

0.831 ± 0.014

5.978 ± 0.037

6.363 ± 0.048

3.496 ± 0.029

9.246 ± 0.041

2.1 ± 0.019

4.639 ± 0.029

2.08 ± 0.034

10.039 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.018

2.125 ± 0.023

5.449 ± 0.029

2.744 ± 0.022

8.213 ± 0.048

4.532 ± 0.026

5.04 ± 0.029

7.157 ± 0.036

1.418 ± 0.022

2.025 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski