Amino acid dipepetide frequency for Rhodobacteraceae bacterium WD3A24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.461AlaAla: 21.461 ± 0.264	1.104AlaCys: 1.104 ± 0.036	7.372AlaAsp: 7.372 ± 0.107	9.641AlaGlu: 9.641 ± 0.118	4.398AlaPhe: 4.398 ± 0.076	13.499AlaGly: 13.499 ± 0.149	2.738AlaHis: 2.738 ± 0.058	5.519AlaIle: 5.519 ± 0.087	2.292AlaLys: 2.292 ± 0.064	15.563AlaLeu: 15.563 ± 0.179	3.873AlaMet: 3.873 ± 0.061	2.276AlaAsn: 2.276 ± 0.05	7.573AlaPro: 7.573 ± 0.118	4.341AlaGln: 4.341 ± 0.065	12.99AlaArg: 12.99 ± 0.165	4.986AlaSer: 4.986 ± 0.087	6.055AlaThr: 6.055 ± 0.079	9.136AlaVal: 9.136 ± 0.117	1.657AlaTrp: 1.657 ± 0.047	2.307AlaTyr: 2.307 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.115CysAla: 1.115 ± 0.034	0.091CysCys: 0.091 ± 0.009	0.573CysAsp: 0.573 ± 0.026	0.467CysGlu: 0.467 ± 0.024	0.293CysPhe: 0.293 ± 0.018	0.936CysGly: 0.936 ± 0.039	0.267CysHis: 0.267 ± 0.015	0.337CysIle: 0.337 ± 0.02	0.121CysLys: 0.121 ± 0.011	0.763CysLeu: 0.763 ± 0.028	0.149CysMet: 0.149 ± 0.012	0.206CysAsn: 0.206 ± 0.017	0.503CysPro: 0.503 ± 0.023	0.168CysGln: 0.168 ± 0.013	0.656CysArg: 0.656 ± 0.026	0.377CysSer: 0.377 ± 0.022	0.436CysThr: 0.436 ± 0.021	0.569CysVal: 0.569 ± 0.026	0.103CysTrp: 0.103 ± 0.011	0.177CysTyr: 0.177 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.964AspAla: 7.964 ± 0.094	0.517AspCys: 0.517 ± 0.025	3.868AspAsp: 3.868 ± 0.09	3.834AspGlu: 3.834 ± 0.084	2.277AspPhe: 2.277 ± 0.054	5.695AspGly: 5.695 ± 0.107	1.396AspHis: 1.396 ± 0.039	3.009AspIle: 3.009 ± 0.06	1.188AspLys: 1.188 ± 0.037	5.763AspLeu: 5.763 ± 0.088	1.504AspMet: 1.504 ± 0.042	1.139AspAsn: 1.139 ± 0.035	3.889AspPro: 3.889 ± 0.067	1.446AspGln: 1.446 ± 0.044	4.584AspArg: 4.584 ± 0.078	2.322AspSer: 2.322 ± 0.049	2.781AspThr: 2.781 ± 0.06	3.85AspVal: 3.85 ± 0.066	1.266AspTrp: 1.266 ± 0.034	1.483AspTyr: 1.483 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	9.588GluAla: 9.588 ± 0.133	0.365GluCys: 0.365 ± 0.018	3.359GluAsp: 3.359 ± 0.068	3.993GluGlu: 3.993 ± 0.09	1.749GluPhe: 1.749 ± 0.041	5.821GluGly: 5.821 ± 0.093	1.243GluHis: 1.243 ± 0.036	3.697GluIle: 3.697 ± 0.062	1.605GluLys: 1.605 ± 0.047	5.616GluLeu: 5.616 ± 0.074	1.897GluMet: 1.897 ± 0.045	1.488GluAsn: 1.488 ± 0.038	2.902GluPro: 2.902 ± 0.065	1.869GluGln: 1.869 ± 0.048	5.54GluArg: 5.54 ± 0.078	2.694GluSer: 2.694 ± 0.052	3.954GluThr: 3.954 ± 0.07	4.435GluVal: 4.435 ± 0.075	0.792GluTrp: 0.792 ± 0.031	1.023GluTyr: 1.023 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.621PheAla: 4.621 ± 0.07	0.364PheCys: 0.364 ± 0.019	2.716PheAsp: 2.716 ± 0.051	2.325PheGlu: 2.325 ± 0.049	1.322PhePhe: 1.322 ± 0.044	3.448PheGly: 3.448 ± 0.071	0.754PheHis: 0.754 ± 0.03	1.508PheIle: 1.508 ± 0.046	0.569PheLys: 0.569 ± 0.025	3.251PheLeu: 3.251 ± 0.083	0.773PheMet: 0.773 ± 0.029	0.905PheAsn: 0.905 ± 0.033	1.543PhePro: 1.543 ± 0.04	0.811PheGln: 0.811 ± 0.03	2.434PheArg: 2.434 ± 0.046	1.819PheSer: 1.819 ± 0.048	1.934PheThr: 1.934 ± 0.046	2.525PheVal: 2.525 ± 0.054	0.542PheTrp: 0.542 ± 0.026	0.798PheTyr: 0.798 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	12.288GlyAla: 12.288 ± 0.137	0.906GlyCys: 0.906 ± 0.033	4.93GlyAsp: 4.93 ± 0.084	5.527GlyGlu: 5.527 ± 0.088	3.596GlyPhe: 3.596 ± 0.06	8.61GlyGly: 8.61 ± 0.118	2.096GlyHis: 2.096 ± 0.044	3.947GlyIle: 3.947 ± 0.081	2.223GlyLys: 2.223 ± 0.066	9.804GlyLeu: 9.804 ± 0.121	2.83GlyMet: 2.83 ± 0.061	1.797GlyAsn: 1.797 ± 0.043	4.268GlyPro: 4.268 ± 0.072	2.797GlyGln: 2.797 ± 0.056	7.942GlyArg: 7.942 ± 0.105	3.758GlySer: 3.758 ± 0.059	4.434GlyThr: 4.434 ± 0.072	6.884GlyVal: 6.884 ± 0.086	1.644GlyTrp: 1.644 ± 0.041	2.181GlyTyr: 2.181 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.716HisAla: 2.716 ± 0.059	0.222HisCys: 0.222 ± 0.014	1.467HisAsp: 1.467 ± 0.039	1.181HisGlu: 1.181 ± 0.033	0.8HisPhe: 0.8 ± 0.029	2.073HisGly: 2.073 ± 0.047	0.557HisHis: 0.557 ± 0.028	0.857HisIle: 0.857 ± 0.027	0.386HisLys: 0.386 ± 0.023	2.044HisLeu: 2.044 ± 0.054	0.5HisMet: 0.5 ± 0.023	0.426HisAsn: 0.426 ± 0.022	1.489HisPro: 1.489 ± 0.036	0.516HisGln: 0.516 ± 0.024	1.556HisArg: 1.556 ± 0.039	0.865HisSer: 0.865 ± 0.03	0.799HisThr: 0.799 ± 0.025	1.632HisVal: 1.632 ± 0.047	0.369HisTrp: 0.369 ± 0.019	0.542HisTyr: 0.542 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	7.026IleAla: 7.026 ± 0.093	0.487IleCys: 0.487 ± 0.023	3.339IleAsp: 3.339 ± 0.067	3.359IleGlu: 3.359 ± 0.065	1.524IlePhe: 1.524 ± 0.041	4.46IleGly: 4.46 ± 0.074	0.923IleHis: 0.923 ± 0.026	1.901IleIle: 1.901 ± 0.053	0.887IleLys: 0.887 ± 0.033	4.084IleLeu: 4.084 ± 0.079	0.936IleMet: 0.936 ± 0.036	1.139IleAsn: 1.139 ± 0.036	2.191IlePro: 2.191 ± 0.047	0.899IleGln: 0.899 ± 0.034	3.296IleArg: 3.296 ± 0.059	2.346IleSer: 2.346 ± 0.059	2.578IleThr: 2.578 ± 0.051	3.43IleVal: 3.43 ± 0.07	0.589IleTrp: 0.589 ± 0.026	0.994IleTyr: 0.994 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	2.68LysAla: 2.68 ± 0.061	0.136LysCys: 0.136 ± 0.011	1.015LysAsp: 1.015 ± 0.034	1.063LysGlu: 1.063 ± 0.041	0.606LysPhe: 0.606 ± 0.025	1.901LysGly: 1.901 ± 0.055	0.408LysHis: 0.408 ± 0.019	1.048LysIle: 1.048 ± 0.036	0.685LysLys: 0.685 ± 0.035	2.07LysLeu: 2.07 ± 0.055	0.556LysMet: 0.556 ± 0.027	0.47LysAsn: 0.47 ± 0.022	1.196LysPro: 1.196 ± 0.039	0.599LysGln: 0.599 ± 0.025	1.694LysArg: 1.694 ± 0.054	1.172LysSer: 1.172 ± 0.041	1.312LysThr: 1.312 ± 0.044	1.485LysVal: 1.485 ± 0.047	0.277LysTrp: 0.277 ± 0.016	0.434LysTyr: 0.434 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	14.524LeuAla: 14.524 ± 0.165	0.919LeuCys: 0.919 ± 0.033	6.602LeuAsp: 6.602 ± 0.099	5.926LeuGlu: 5.926 ± 0.086	3.339LeuPhe: 3.339 ± 0.074	9.089LeuGly: 9.089 ± 0.13	1.992LeuHis: 1.992 ± 0.046	4.666LeuIle: 4.666 ± 0.086	2.186LeuLys: 2.186 ± 0.054	8.839LeuLeu: 8.839 ± 0.131	2.622LeuMet: 2.622 ± 0.058	2.082LeuAsn: 2.082 ± 0.05	5.507LeuPro: 5.507 ± 0.072	2.376LeuGln: 2.376 ± 0.058	8.382LeuArg: 8.382 ± 0.108	5.921LeuSer: 5.921 ± 0.089	5.332LeuThr: 5.332 ± 0.088	6.907LeuVal: 6.907 ± 0.095	1.319LeuTrp: 1.319 ± 0.039	1.862LeuTyr: 1.862 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	3.575MetAla: 3.575 ± 0.064	0.158MetCys: 0.158 ± 0.013	1.276MetAsp: 1.276 ± 0.037	1.48MetGlu: 1.48 ± 0.037	0.685MetPhe: 0.685 ± 0.029	2.271MetGly: 2.271 ± 0.056	0.499MetHis: 0.499 ± 0.022	1.369MetIle: 1.369 ± 0.039	0.721MetLys: 0.721 ± 0.026	2.614MetLeu: 2.614 ± 0.05	0.704MetMet: 0.704 ± 0.032	0.702MetAsn: 0.702 ± 0.025	1.482MetPro: 1.482 ± 0.045	0.768MetGln: 0.768 ± 0.026	2.308MetArg: 2.308 ± 0.048	1.572MetSer: 1.572 ± 0.036	1.95MetThr: 1.95 ± 0.047	1.771MetVal: 1.771 ± 0.05	0.233MetTrp: 0.233 ± 0.017	0.336MetTyr: 0.336 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.745AsnAla: 2.745 ± 0.053	0.216AsnCys: 0.216 ± 0.016	1.141AsnAsp: 1.141 ± 0.033	1.029AsnGlu: 1.029 ± 0.033	0.793AsnPhe: 0.793 ± 0.033	1.847AsnGly: 1.847 ± 0.049	0.433AsnHis: 0.433 ± 0.017	1.156AsnIle: 1.156 ± 0.037	0.439AsnLys: 0.439 ± 0.023	2.068AsnLeu: 2.068 ± 0.043	0.578AsnMet: 0.578 ± 0.028	0.573AsnAsn: 0.573 ± 0.025	1.669AsnPro: 1.669 ± 0.04	0.537AsnGln: 0.537 ± 0.023	1.62AsnArg: 1.62 ± 0.041	0.912AsnSer: 0.912 ± 0.036	1.069AsnThr: 1.069 ± 0.035	1.524AsnVal: 1.524 ± 0.041	0.351AsnTrp: 0.351 ± 0.02	0.547AsnTyr: 0.547 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.53ProAla: 7.53 ± 0.108	0.328ProCys: 0.328 ± 0.018	4.313ProAsp: 4.313 ± 0.077	4.763ProGlu: 4.763 ± 0.069	1.878ProPhe: 1.878 ± 0.042	5.357ProGly: 5.357 ± 0.086	1.105ProHis: 1.105 ± 0.034	1.829ProIle: 1.829 ± 0.044	0.975ProLys: 0.975 ± 0.034	4.912ProLeu: 4.912 ± 0.082	1.271ProMet: 1.271 ± 0.037	1.048ProAsn: 1.048 ± 0.034	3.042ProPro: 3.042 ± 0.08	1.618ProGln: 1.618 ± 0.048	3.855ProArg: 3.855 ± 0.072	2.25ProSer: 2.25 ± 0.048	2.112ProThr: 2.112 ± 0.041	4.511ProVal: 4.511 ± 0.068	0.707ProTrp: 0.707 ± 0.028	1.083ProTyr: 1.083 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.827GlnAla: 3.827 ± 0.066	0.188GlnCys: 0.188 ± 0.014	1.337GlnAsp: 1.337 ± 0.034	1.586GlnGlu: 1.586 ± 0.041	0.902GlnPhe: 0.902 ± 0.031	2.489GlnGly: 2.489 ± 0.05	0.47GlnHis: 0.47 ± 0.02	1.61GlnIle: 1.61 ± 0.041	0.677GlnLys: 0.677 ± 0.024	2.343GlnLeu: 2.343 ± 0.054	0.857GlnMet: 0.857 ± 0.029	0.622GlnAsn: 0.622 ± 0.026	1.563GlnPro: 1.563 ± 0.041	0.895GlnGln: 0.895 ± 0.044	2.26GlnArg: 2.26 ± 0.049	1.408GlnSer: 1.408 ± 0.043	1.529GlnThr: 1.529 ± 0.041	1.979GlnVal: 1.979 ± 0.044	0.364GlnTrp: 0.364 ± 0.02	0.534GlnTyr: 0.534 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	11.592ArgAla: 11.592 ± 0.14	0.568ArgCys: 0.568 ± 0.025	4.824ArgAsp: 4.824 ± 0.071	5.125ArgGlu: 5.125 ± 0.087	3.03ArgPhe: 3.03 ± 0.053	6.234ArgGly: 6.234 ± 0.082	1.911ArgHis: 1.911 ± 0.047	4.147ArgIle: 4.147 ± 0.07	1.892ArgLys: 1.892 ± 0.054	9.443ArgLeu: 9.443 ± 0.133	2.271ArgMet: 2.271 ± 0.048	1.689ArgAsn: 1.689 ± 0.039	4.414ArgPro: 4.414 ± 0.083	2.32ArgGln: 2.32 ± 0.052	7.618ArgArg: 7.618 ± 0.121	3.191ArgSer: 3.191 ± 0.049	3.32ArgThr: 3.32 ± 0.055	5.73ArgVal: 5.73 ± 0.082	1.151ArgTrp: 1.151 ± 0.034	1.663ArgTyr: 1.663 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	5.687SerAla: 5.687 ± 0.084	0.383SerCys: 0.383 ± 0.023	2.833SerAsp: 2.833 ± 0.048	2.755SerGlu: 2.755 ± 0.058	1.93SerPhe: 1.93 ± 0.05	5.049SerGly: 5.049 ± 0.074	0.947SerHis: 0.947 ± 0.032	1.964SerIle: 1.964 ± 0.048	0.89SerLys: 0.89 ± 0.032	4.28SerLeu: 4.28 ± 0.073	1.128SerMet: 1.128 ± 0.032	1.055SerAsn: 1.055 ± 0.035	2.486SerPro: 2.486 ± 0.048	1.274SerGln: 1.274 ± 0.037	3.444SerArg: 3.444 ± 0.057	2.067SerSer: 2.067 ± 0.058	2.072SerThr: 2.072 ± 0.049	3.361SerVal: 3.361 ± 0.059	0.631SerTrp: 0.631 ± 0.028	1.086SerTyr: 1.086 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	6.23ThrAla: 6.23 ± 0.093	0.391ThrCys: 0.391 ± 0.019	2.877ThrAsp: 2.877 ± 0.051	2.786ThrGlu: 2.786 ± 0.054	1.684ThrPhe: 1.684 ± 0.047	5.383ThrGly: 5.383 ± 0.077	1.074ThrHis: 1.074 ± 0.032	2.316ThrIle: 2.316 ± 0.046	0.849ThrLys: 0.849 ± 0.033	5.716ThrLeu: 5.716 ± 0.091	1.125ThrMet: 1.125 ± 0.032	1.047ThrAsn: 1.047 ± 0.032	3.452ThrPro: 3.452 ± 0.062	1.249ThrGln: 1.249 ± 0.037	3.987ThrArg: 3.987 ± 0.063	2.201ThrSer: 2.201 ± 0.044	2.515ThrThr: 2.515 ± 0.056	3.896ThrVal: 3.896 ± 0.064	0.612ThrTrp: 0.612 ± 0.027	1.007ThrTyr: 1.007 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	9.593ValAla: 9.593 ± 0.109	0.621ValCys: 0.621 ± 0.026	3.829ValAsp: 3.829 ± 0.071	4.873ValGlu: 4.873 ± 0.077	2.84ValPhe: 2.84 ± 0.057	5.378ValGly: 5.378 ± 0.069	1.329ValHis: 1.329 ± 0.038	4.013ValIle: 4.013 ± 0.068	1.461ValLys: 1.461 ± 0.036	7.277ValLeu: 7.277 ± 0.095	2.056ValMet: 2.056 ± 0.047	1.757ValAsn: 1.757 ± 0.045	3.562ValPro: 3.562 ± 0.059	1.809ValGln: 1.809 ± 0.038	4.859ValArg: 4.859 ± 0.073	3.702ValSer: 3.702 ± 0.069	4.519ValThr: 4.519 ± 0.072	5.723ValVal: 5.723 ± 0.09	0.98ValTrp: 0.98 ± 0.031	1.386ValTyr: 1.386 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.595TrpAla: 1.595 ± 0.042	0.115TrpCys: 0.115 ± 0.01	0.641TrpAsp: 0.641 ± 0.03	0.718TrpGlu: 0.718 ± 0.026	0.488TrpPhe: 0.488 ± 0.024	1.015TrpGly: 1.015 ± 0.032	0.359TrpHis: 0.359 ± 0.016	0.627TrpIle: 0.627 ± 0.029	0.35TrpLys: 0.35 ± 0.021	1.829TrpLeu: 1.829 ± 0.047	0.404TrpMet: 0.404 ± 0.019	0.354TrpAsn: 0.354 ± 0.022	0.76TrpPro: 0.76 ± 0.026	0.612TrpGln: 0.612 ± 0.025	1.397TrpArg: 1.397 ± 0.035	0.747TrpSer: 0.747 ± 0.029	0.738TrpThr: 0.738 ± 0.029	0.917TrpVal: 0.917 ± 0.03	0.247TrpTrp: 0.247 ± 0.017	0.264TrpTyr: 0.264 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.426TyrAla: 2.426 ± 0.049	0.226TyrCys: 0.226 ± 0.014	1.464TyrAsp: 1.464 ± 0.041	1.197TyrGlu: 1.197 ± 0.04	0.83TyrPhe: 0.83 ± 0.033	1.905TyrGly: 1.905 ± 0.04	0.495TyrHis: 0.495 ± 0.025	0.867TyrIle: 0.867 ± 0.028	0.408TyrLys: 0.408 ± 0.019	2.113TyrLeu: 2.113 ± 0.052	0.439TyrMet: 0.439 ± 0.023	0.471TyrAsn: 0.471 ± 0.024	1.0TyrPro: 1.0 ± 0.034	0.534TyrGln: 0.534 ± 0.024	1.71TyrArg: 1.71 ± 0.047	1.015TyrSer: 1.015 ± 0.032	0.958TyrThr: 0.958 ± 0.034	1.303TyrVal: 1.303 ± 0.038	0.345TyrTrp: 0.345 ± 0.019	0.544TyrTyr: 0.544 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3201 proteins (994201 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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