Desulfitobacterium hafniense (strain Y51)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfitobacterium; Desulfitobacterium hafniense

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q24XF3|Q24XF3_DESHY ABC transmembrane type-1 domain-containing protein OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=DSY1500 PE=3 SV=1
MM1 pKa = 6.87TAEE4 pKa = 3.96IMEE7 pKa = 4.54KK8 pKa = 10.96AEE10 pKa = 3.9VLAAAIAKK18 pKa = 9.9SVEE21 pKa = 4.03LQNLRR26 pKa = 11.84STEE29 pKa = 3.93EE30 pKa = 3.71AMMADD35 pKa = 3.85EE36 pKa = 4.44QAQQIIADD44 pKa = 4.27FQNEE48 pKa = 3.88QQRR51 pKa = 11.84VYY53 pKa = 10.49EE54 pKa = 4.17LQAQGQEE61 pKa = 4.27LTDD64 pKa = 3.75EE65 pKa = 4.34VQQAIDD71 pKa = 3.25AMEE74 pKa = 4.57AKK76 pKa = 10.58VEE78 pKa = 4.27GYY80 pKa = 9.76PPIAAYY86 pKa = 9.79LQAQEE91 pKa = 4.81QFTKK95 pKa = 10.38MLDD98 pKa = 3.67TINGVLAQAIANDD111 pKa = 4.42PNGGGCSCDD120 pKa = 3.58TGCSGCGGSCSS131 pKa = 3.45

Molecular weight: 13.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q24NK3|Q24NK3_DESHY HTH arsR-type domain-containing protein OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=DSY4600 PE=4 SV=1
MM1 pKa = 7.46LRR3 pKa = 11.84YY4 pKa = 9.63LLNPKK9 pKa = 9.96YY10 pKa = 7.46PTPLNRR16 pKa = 11.84KK17 pKa = 8.72QSGCEE22 pKa = 3.96QKK24 pKa = 10.54SGPHH28 pKa = 4.86NRR30 pKa = 11.84VRR32 pKa = 11.84EE33 pKa = 4.08QSLSAAWVRR42 pKa = 11.84NRR44 pKa = 11.84LNLLLRR50 pKa = 11.84EE51 pKa = 4.53AAQSAPSDD59 pKa = 3.58ASLHH63 pKa = 5.82PLHH66 pKa = 6.8PRR68 pKa = 11.84HH69 pKa = 6.41RR70 pKa = 11.84PLACAEE76 pKa = 4.22TGSVVV81 pKa = 2.93

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5014

0

5014

1600940

35

3013

319.3

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.204 ± 0.035

1.193 ± 0.017

4.834 ± 0.023

7.081 ± 0.037

4.056 ± 0.024

7.682 ± 0.04

1.929 ± 0.015

7.016 ± 0.029

5.836 ± 0.028

10.437 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.019

3.77 ± 0.023

4.14 ± 0.025

3.921 ± 0.024

4.8 ± 0.029

5.822 ± 0.026

5.168 ± 0.031

6.833 ± 0.027

1.134 ± 0.012

3.411 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski